This study aims to analyze the impact of transformational leadership and culture on safety behavior through the motivation of crew members at PT. Pelayaran Karana Line branch in Pulau Batam. Using a quantitative research method, data were collected through questionnaires distributed to the crew members. The results of the study indicate that transformational leadership and organizational culture have a positive and significant impact on safety behavior, mediated by the motivation of the crew. These findings provide practical implications for the company's management in enhancing workplace safety onboard ships.