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All Journal Jurnal Veteriner Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Public Health Research and Community Health Development (JPH RECODE) Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Health Dynamics

Yudhana, Aditya

Universitas Airlangga

Author-ID : 6083891

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Karakterisasi Protein VirB4 Brucella abortus Isolat Lokal dengan Teknik Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Ratih Novita Praja; Didik Handijatno; Setiawan Koesdarto; Aditya Yudhana
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Brucellosis is a zoonotic disease cause by pathogenic bacteria of the genus Brucella. The disease infects livestock mammals such as cows, goats, pigs, and including humans. Brucella abortus has several potential virulence factors, i.e. Proteins VirB. Type IV secretion system (T4SS) which is a combination of 12 proteins from VirB1-VirB11 and VirD4. Brucella can survive for long periods in the environment despite the limitations of nutrients and oxygen. This study aims to characterize the protein VirB4 of local isolate of B. abortus using SDS PAGE (Sodium Dodecly sulfate-polyacrylamide gel electrophoresis). The results showed that the protein contained 10 protein bands with a molecular weight of 158.93; 110.89; 99.931; 70.60; 64.61; 59.25; 45.32; 42.35; 23.63; and 16.70 kDa, respectively. Protein VirB4 of the local isolate of B. abortus have a molecular weight of 59.25 kDa. ABSTRAK Brucellosis merupakan salah satu penyakit zoonosis yang disebabkan oleh bakteri patogen genus Brucella. Penyakit ini menyerang hewan ternak mamalia seperti sapi, kambing, babi, dan dapat menular ke manusia. Bakteri Brucella abortus memiliki faktor virulensi potensial yaitu protein VirB. Type IV Secretion System (T4SS) merupakan gabungan dari 12 protein yaitu VirB1–VirB11 dan VirD4. Brucella dapat bertahan hidup lama di dalam lingkungan meskipun memiliki keterbatasan nutrisi dan oksigen. Penelitian ini dilakukan untuk karakterisasi protein VirB4 B. abortus isolat lokal dengan metode Sodium Dodecly Sulfate-Polyacrylamide Gel Electrophoresis (SDS PAGE). Hasil karakterisasi protein B. abortus isolat lokal dengan teknik SDS-PAGE terdapat 10 pita protein dengan bobot molekul 158,93; 110,89; 99,931; 70,60; 64,61; 59,25; 45,32; 42,35; 23,63; dan 16,70 kDa. Simpulan penelitian ini adalah terdapat protein VirB4 B. abortus isolat lokal yang mempunyai bobot molekul 59,25 kDa.

Deteksi Gen Resisten Insektisida Organofosfat pada Aedes aegypti di Banyuwangi, Jawa Timur Menggunakan Polymerase Chain Reaction Aditya Yudhana; Ratih Novita Praja; Maya Nurwartanti Yunita
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Banyuwangi is still listed as areas not completely free from hemorrhagic dengue fever outbreak. Changes in the genome of Aedes aegypti mosquito as the main vector of dengue virus is estimated to be the cause of difficulty in controlling the disease. Expression of Voltage Gated Sodium Channel (VGSC) genes can be used as an important indication of Aedes aegypti mosquitoes resistancy to certain groups insecticides. The purpose of this study was to detect the Voltage Gated Sodium Channel (VGSC) genes coding in Aedes aegypti using Polymerase Chain Reaction (PCR); as it is related to the mechanism of resistance to organophosphate insecticides groups. Results of resistency by susceptibility test showed that Aedes aegypti mosquito samples from three district ie. Tegaldlimo, Purwoharjo, and Banyuwangi were resistant to malathion 0.8% insecticide (organophosphates group). PCR results detected gen band with 250 bp length, which confirmed that insecticide resistance gene has been detected in the tested samples. Based on this study results, it is recommended to use alternative insecticides apart from organophosphate group for dengue vector control in Banyuwangi. ABSTRAK Kabupaten Banyuwangi masih tercatat sebagai daerah yang belum sepenuhnya bebas dari wabah demam berdarah dengue (DBD). Perubahan genom nyamuk Aedes aegypti sebagai vektor utama virus dengue diperkirakan menjadi penyebab sulitnya pengendalian penyakit DBD. Ekspresi gen Voltage Gated Sodium Channel (VGSC) dapat dijadikan indikasi penting sejauh mana perkembangan nyamuk Aedes aegypti resisten terhadap insektisida golongan tertentu. Tujuan penelitian ini adalah untuk mendeteksi gen penyandi VGSC pada nyamuk A. aegypti yang berkaitan dengan mekanisme resistensi terhadap insektisida golongan organofosfat menggunakan teknik Polymerase Chain Reaction (PCR). Hasil uji resistensi susceptibility test menunjukkan sampel nyamuk A. aegypti dari tiga kecamatan di Kabupaten Banyuwangi yaitu Kecamatan Tegaldlimo, Purwoharjo, dan Banyuwangi resisten terhadap insektisida malathion 0,8 % (kelompok organofosfat). Hasil deteksi gen melalui PCR didapatkan band yang muncul dengan panjang 250 bp. Hal tersebut menunjukkan bahwa gen pembawa resistensi insektisida (VGSC) telah terdeteksi pada sampel yang diuji. Berdasarkan hasil penelitian, disarankan untuk pengendalian vektor DBD di Kabupaten Banyuwangi dengan menggunakan alternatif insektisida selain dari golongan organofosfat.

Co-Authors Agustono, Bodhi Al-Madinah, Wardatul Qoryah Aliyyah Revinda Bima Putri Amanda, Nadya Amelia, Ezanti Nur Anastasya, Anastasya Andini, Zahrotul Andriyani, Anita APRIL HARI WARDHANA Ardy, Carolina Arifin, Khoirul Arimbi Audina Putri Geraldine Boedi Setiawan Chaerunisa, Nadya Amanda Chaerunissa, Nadya Amanda Chusniati, Sri Citra Kurnia Putri Danuarta, Muhammad Fernanda Didik Handijatno Didik Handijatno Dini, Sayyida Kamila Endang Suprihati Erma Safitri Fachrur Rozi Faisal Fikri Farezi, Reza Adrio Farhan, Muhammad Riesta Febriana, Melati Octavia Febriyatin, Fierdausi Nur’Aini Fiqih, Adam Fahmi Firmansyah, Januar Fransiska Okta Zania Fransiska, Eunike Melanda Haditanojo , Wiyanto Haditanojo, Wiyanto Hairi, Farizah Mohd Hamonangan, Jonathan Mark Hani Plumeriastuti Herdiansyah, Akbar Dimas Ichi Arum Galerani Insani, Alivia Khairina Ira Sari Yudaniayanti Iwan Sahrial Hamid Jayanti Dian Eka Sari, Jayanti Dian Karami, Farhan Kenconojati, Hapsari Khairunnisa, Hanifa Khansa Koesdarto , Setiawan Kristina, Silvia Martha Kurnianto, Muhammad Aqil Kusnoto Kusnoto, Kusnoto Kusuma, Widya Ayu Kusumarini, Shelly Kusumastuti, Imbi Mandagi, Ayik Mirayanti Maulana, Muhammad Rizal Megawati, Neneng Ismi Mufasirin Muhammad Thohawi Elziyad Purnama Mumtazi, Fauzan Muslimah, Bintang Mustofa Helmi Effendi Nor, Roslindawani Md Nurfaizah, Diza Ulya Nurhidayah, Nanis Nurin Ardhiani Nurwartanti, Maya Nusdianto Triakoso Oktaviana, Vivi Panjaitan, Cahaya Poedji Hastutiek Pradika, Adhitya Yhoga Praja, Ratih Novita Praja, Shifa Salsabilla Pramesti, Nugraheni Anggara Prameswari, Febriana Hawa Prameswari, Nindya Pradnya Prameswari, Ratna Ajeng Prasetyo, Dhenatra Rifqy Prastiya, Ragil Angga Pribadi, Adinda Dilla Puspikawati, Septa Indra Putri, Firrial Eksa Maulidania Putri, Valerie Brilianda Rahmadani Rahmadani Rahmadhani, Nurmitasari Rahmahani, Jola Rahmawati, Anlisia Ramadhan, Alfian Dzaka Fadhil Ramadhanny, Syahrul Novenda Romadhona, Ma'rifatunnisa' Ryanka Edila Saksono, Bayu Salahuddin, Muhammad Soleh Saliluddin, Suhainizam Bin Muhamad Salsabila Putri, Elma Samosir, Sabrina Hatoguan Sandy, Berliana Dwi Nandita Santosa, Gusti Ayu Illiyin Putri Saputro, Amung Logam Sarudji, Suryanie Setiawan Koesdarto Setiawati, Puji Aris Soeharsono Soeharsono Soetojo Soetojo Suherni Susilowati Sukarno, Gerda Sulaeman, Nur Sa'adah Sunarso, Agus Suroiyah, Fifi Anik Susilo, Rahadian Indarto Suwarno Suwarno Tanjung, Aldi Gusnizar Rizaldy Tatik Hernawati Tiara Wandira Hariyanto Tjuk Imam Restiadi Tyasningsih, Wiwiek Umama, Ayu Riza Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Wijaya, Nanik Sianita Wisesa, Ida Bagus Gde Rama Wiwik Misaco Yuniarti Yeni Dhamayanti Yesica, Reza Yulianti, Yunika Tri Yulianto, Rangga Yumna, Raissa Amelia Yunita, Maya Nurwartanti Zahrudin, Elham Zulianti, Brillia

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