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First case report of Salmonella spp. infection in Homalopsis buccata snake: Laporan Pertama Kasus Infeksi Salmonella spp. pada Ular Homalopsis buccata Tiara Wandira Hariyanto; Amelia, Ezanti Nur; Tyasningsih, Wiwiek; Rahmahani, Jola; Praja, Ratih Novita; Yudhana, Aditya; Nurwartanti, Maya; Fikri, Faisal
Media Kedokteran Hewan Vol. 34 No. 2 (2023): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v34i2.2023.128-134

Abstract

Reptiles are a source of transmission of pathogenic bacteria to humans. Salmonella spp. are Gram-negative bacteria that are commonly found in the digestive tract of snakes. This study was conducted on Homalopsis buccata snakes with samples obtained by oral swabs and cloacal swabs on snakes. Isolation of Salmonella spp. carried out on Tetrathionate Broth media enrichment and will be inoculated on specific media in the form of Salmonella Shigella Agar media. Identification of bacteria was carried out by Gam staining and Indole, Methyl Red, Voges Proskauer and Citrate test. This study revealed the presence of Salmonella spp. in the snake Homalopsis buccata.
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Yudhana, Aditya; Praja, Ratih Novita; Yunita, Maya Nurwartanti
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.7625

Abstract

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).
CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Praja, Ratih Novita; Handijatno, Didik; Koesdarto, Setiawan; Yudhana, Aditya
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
Pentahelix Approach in Community Empowerment-Based Ecotourism: Turtle Conservation and Waste Management on Santen Island Beach, Banyuwangi Jayanti Dian Eka Sari; Aditya Yudhana; Melati Octavia Febriana; Syahrul Novenda Ramadhanny; Firrial Eksa Maulidania Putri; Suhainizam Bin Muhamad Saliluddin; Ratih Novita Praja
Health Dynamics Vol 1, No 12 (2024): December 2024
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd11205

Abstract

Background: Santen Island Beach, Banyuwangi, faces significant challenges from waste mismanagement and its impact on marine ecosystems, particularly the endangered sea turtles. This study addresses these issues through a community empowerment program grounded in the Pentahelix framework, engaging stakeholders from government, academia, businesses, communities, and media. The program, active since 2022, aims to integrate sustainable waste management and turtle conservation into community development. Methods: The study employed a community empowerment approach, incorporating waste segregation, educational tourism initiatives, and turtle conservation training. Collaborations with local organizations like the Banyuwangi Sea Turtle Foundation and waste management entities ensured resource and expertise mobilization. Activities included waste alms programs and sea turtle egg relocation to sandless hatching boxes, supplemented by training and social media campaigns. Results: The program achieved key milestones, such as forming empowered cadres for waste management and turtle conservation, introducing effective waste segregation practices, and increasing hatchling survival rates through innovative relocation techniques. Outputs included community-led eco-tourism branding, scientific and media publications, and economic benefits for local stakeholders. Conclusion: This initiative highlights the potential of integrating environmental conservation and economic empowerment within a sustainable development framework. By leveraging the Pentahelix model, the program fosters local ownership and long-term impact, addressing SDG goals related to responsible consumption and marine ecosystem preservation. Continued efforts, such as enhancing cadre competencies and optimizing ecotourism branding, are recommended for sustained outcomes.
Co-Authors Agustono, Bodhi Al-Madinah, Wardatul Qoryah Aliyyah Revinda Bima Putri Amanda, Nadya Amelia, Ezanti Nur Anastasya, Anastasya Andini, Zahrotul Andriyani, Anita APRIL HARI WARDHANA Ardy, Carolina Arifin, Khoirul Arimbi Audina Putri Geraldine Boedi Setiawan Chaerunisa, Nadya Amanda Chaerunissa, Nadya Amanda Chusniati, Sri Citra Kurnia Putri Danuarta, Muhammad Fernanda Didik Handijatno Dini, Sayyida Kamila Endang Suprihati Erma Safitri Fachrur Rozi Faisal Fikri Farezi, Reza Adrio Farhan, Muhammad Riesta Febriyatin, Fierdausi Nur’Aini Fiqih, Adam Fahmi Firmansyah, Januar Firrial Eksa Maulidania Putri Fransiska Okta Zania Fransiska, Eunike Melanda Haditanojo , Wiyanto Haditanojo, Wiyanto Hairi, Farizah Mohd Hamonangan, Jonathan Mark Hani Plumeriastuti Herdiansyah, Akbar Dimas Ichi Arum Galerani Insani, Alivia Khairina Ira Sari Yudaniayanti Iwan Sahrial Hamid Jayanti Dian Eka Sari, Jayanti Dian Karami, Farhan Kenconojati, Hapsari Khairunnisa, Hanifa Khansa Koesdarto , Setiawan Kristina, Silvia Martha Kurnianto, Muhammad Aqil Kusnoto Kusnoto, Kusnoto Kusuma, Widya Ayu Kusumarini, Shelly Kusumastuti, Imbi Mandagi, Ayik Mirayanti Maulana, Muhammad Rizal Megawati, Neneng Ismi Melati Octavia Febriana Mufasirin Muhammad Thohawi Elziyad Purnama Mumtazi, Fauzan Muslimah, Bintang Mustofa Helmi Effendi Nor, Roslindawani Md Nurfaizah, Diza Ulya Nurhidayah, Nanis Nurin Ardhiani Nurwartanti, Maya Nusdianto Triakoso Oktaviana, Vivi Panjaitan, Cahaya Poedji Hastutiek Pradika, Adhitya Yhoga Praja, Ratih Novita Praja, Shifa Salsabilla Pramesti, Nugraheni Anggara Prameswari, Febriana Hawa Prameswari, Nindya Pradnya Prameswari, Ratna Ajeng Prasetyo, Dhenatra Rifqy Prastiya, Ragil Angga Pribadi, Adinda Dilla Puspikawati, Septa Indra Putri, Valerie Brilianda Rahmadani Rahmadani Rahmadhani, Nurmitasari Rahmahani, Jola Rahmawati, Anlisia Ramadhan, Alfian Dzaka Fadhil Romadhona, Ma'rifatunnisa' Ryanka Edila Saksono, Bayu Salahuddin, Muhammad Soleh Salsabila Putri, Elma Samosir, Sabrina Hatoguan Sandy, Berliana Dwi Nandita Santosa, Gusti Ayu Illiyin Putri Saputro, Amung Logam Setiawan Koesdarto Setiawati, Puji Aris Soeharsono Soeharsono Soetojo Soetojo Suhainizam Bin Muhamad Saliluddin Suherni Susilowati Sukarno, Gerda Sulaeman, Nur Sa'adah Sunarso, Agus Suroiyah, Fifi Anik Suryanie Sarudji Susilo, Rahadian Indarto Suwarno Suwarno Syahrul Novenda Ramadhanny Tanjung, Aldi Gusnizar Rizaldy Tatik Hernawati Tiara Wandira Hariyanto Tjuk Imam Restiadi Tyasningsih, Wiwiek Umama, Ayu Riza Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Wijaya, Nanik Sianita Wisesa, Ida Bagus Gde Rama Wiwik Misaco Yuniarti Yeni Dhamayanti Yesica, Reza Yulianti, Yunika Tri Yulianto, Rangga Yumna, Raissa Amelia Yunita, Maya Nurwartanti Zahrudin, Elham Zulianti, Brillia