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All Journal HAYATI Journal of Biosciences Majalah Kedokteran Bandung Pharmaciana: Jurnal Kefarmasian Journal of Mathematical and Fundamental Sciences Indonesian Journal of Biotechnology Global Medical and Health Communication Science and Technology Indonesia Molecular and Cellular Biomedical Sciences (MCBS) Journal Of Vocational Health Studies Pharmaceutical Sciences and Research (PSR) Folia Medica Indonesiana
Hanna Sari Widya Kusuma, Hanna Sari Widya
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Journal : Science and Technology Indonesia

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Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Hadiprasetyo, Dhanar Septyawan; Surakusumah, Wahyu
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.228-237

Abstract

Leukemia, characterized by abnormal leukocyte proliferation, ranks ninth in Indonesia as the most common cancer. While treatments such as chemotherapy and radiation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing various parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milling and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment. Cytotoxicity was evaluated using WST-8 assays, ROS levels were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT-PCR. MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology. MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions. The findings suggest potential implications for MPN in targeting leukemia cells. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.
Potential of Bitter Melon (Momordica charantia L.) Extract for Chronic Kidney Disease Based on In Vitro Study via TGF/SMADs Signaling, Antioxidant, Antiinflammation, Apoptosis Inducer Activities Prahastuti, Sijani; Rahardja, Fanny; Wargasetia, Teresa Liliana; Zahiroh, Fadhilah Haifa; Sabrina, Adilah Hafizha Nur; Kusuma, Hanna Sari Widya; Azis, Rizal; Hadiprasetyo, Dhanar Septyawan; Ningrum, Siti Ratu Rahayu; Widowati, Wahyu; Sarwono, Sylvie
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.538-551

Abstract

Chronic kidney disease (CKD) is a physiological abnormality in the kidneys whose prevalence is expected to continue to increase. On the other hand, Bitter melon (Momordica charantia L.) is known to have the potential to manage CKD. This study explores the compound content of M. charantia ethanol extract (MCEE) and its potential for CKD based on in vitro assays. To model chronic kidney disease (CKD), SV40 MES-13 (mouse glomerular mesangial) cells were exposed for 3 days to 20 mM glucose. After glucose induction, the cells were subjected with different concentrations of MCEE (Momordica charantia L. ethanolic extract). The chemical profile of MCEE was analyzed using LC/MS-MS. Cell viability was examined through the WST assay, while intracellular ROS and apoptosis levels were measured by flowcytometry. Colorimetry was used to analyze SOD, MDA, and CAT levels. ELISA was used to analyze inflammatory proteins (TGF-β 1, IL-6, TNF-α, IL-1β ) levels. Meanwhile, the relative gene expression of SMAD-2, SMAD-3, SMAD-4, SMAD-7 was examined through qRT-PCR. The results exhibited that MCEE contains cucurbitane p-coumaric, ferulic acid, caffeic acid, gallic acid, chlorogenic acid, and epicatechin. MCEE was also known to be non-toxic to SV40 MES-13 cells. In addition, MCEE reduced intracellular ROS levels, MDA, necrosis levels, and inflammatory proteins, while also regulating SMAD-2, SMAD-3, and SMAD-4 gene expression. MCEE increased levels of CAT, and SOD, and regulated SMAD-7 gene expression in the CKD cells model. The most effective MCEE is MCEE 50 μg/mL. MCEE demonstrated potential as a CKD treatment based on in vitro studies through TGF/SMADs signaling activity, antioxidant, anti-inflammatory, and apoptosis inducer.
Co-Authors Afifah Bambang Sutjiatmo, Afifah Bambang Agung Novianto, Agung Arif, Julio Arinta, Yukko Arrahmani, Betharie Cendera Aryenti, Aryenti Azis, Rizal Batubara, Lilian Damayanti, Ndaru Andri Dewi Nurul, Dewi Dewi, Intan Keumala Dewi, Nindia Salsabila Mia Djaja Rusmana Edriayani, Novi Edwin Setiabudi Edy Fachrial Eltania, The Fransiska Endah Purnamasari Fahrauk, M. Fanny Rahardja Fauziyah, Nida Firyal Ferdinand, Sahna Gunadi, Michael Sebastian Gunanegara, Rimonta Febby Hadiprasetyo, Dhanar Septyawan Halim, Nathanael Hanum, Nur Adiba Hartini Tiono Irfan Syarif, Irfan Ismalayani, Ismalayani Jo Suherman Johan Lucianus Julio Arif Lister, I Nyoman Ehrich Lusiana Darsono Malau, Reghita Claudia Marisca Evalina Gondokesumo, Marisca Evalina Marpaung, Hans Hotma Mars, Nicholas Mulyasari, Tira Endang Nafik, Said Nasution, Ali Napiah Nindia Salsabila Mia Dewi Nindya, Faradhina Salfa Ningrum, Siti Ratu Rahayu Novilla, Arina Nunung Ainur Rahmah Obeng, Selonan Pastari, Marta Philips Onggowidjaja Purwoko, Agus Rahmad Aswin Juliansyah, Rahmad Aswin Rita Tjokropranoto Rizal Rizal Sabrina, Adilah Hafizha Nur Sachrowardi, Qomariyah Romadhiyani Salfa Nindya, Faradhina Santiadi, Sherly Sarwono, Sylvie Sijani Prahastuti Siregar, Irma Dolsyeria Solihat, Ananda Khairunisa Sosiawan, Insan Sri Utami Suci Nar Vikasari, Suci Nar Sukandar, Elin Y. Susi Endrini Sutjiatmo, Afifah B Syokumawena, Syokumawena Teddy Marcus Zakaria Teresa Liliana Wargasetia Triharsiwi, Dwi Nur Utomo, Herry S vera vera, vera Wahyu Surakusumah WAHYU WIDOWATI Zahiroh, Fadhilah Haifa