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All Journal Jurnal Pelita Pendidikan Saintifika Jurnal Biologi dan Pembelajarannya (JBP) Biota Jurnal Pendidikan Biologi Indonesia Bio Ilmi: Jurnal Pendidikan Pedagogi Hayati Jurnal Pendidikan Biologi Biosfer: Jurnal Pendidikan Biologi Jurnal SOLMA Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Assimilation: Indonesian Journal of Biology Education JPM PAMBUDI JPBIO (Jurnal Pendidikan Biologi) Jurnal Biotek BIOSFER : Jurnal Biologi dan Pendidikan Biologi Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Bioscientist : Jurnal Ilmiah Biologi IJORER : International Journal of Recent Educational Research Proceeding ISETH (International Summit on Science, Technology, and Humanity) SAINTIFIKA JIPI (Jurnal IPA dan Pembelajaran IPA) JPM Pambudi KOPEMAS Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Trio Ageng Prayitno
Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Rhizosphere Bacteria Plumeria acuminata Increases Growth of Zea mays After Root Inoculation Insani, Qori Tsaniyah Ainun; Rahayu, Triastuti; Widianto, Donny; Listiawati, Vina; Mumpuni, Kistantia Elok; Prayitno, Trio Ageng; Sidiq*, Yasir
Jurnal IPA & Pembelajaran IPA Vol 7, No 4 (2023): DECEMBER 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jipi.v7i4.34658

Abstract

The bacteria found in the rhizosphere are called plant growth promoting rhizobacteria (PGPR), namely bacteria that can stimulate plant growth by producing growth hormones, one of which is the hormone indole acetic acid (IAA) which plays a role in regulating plant growth and development. Five isolates of rhizosphere bacteria were isolated on frangipani roots (P26, P31, P36, P37 and P38). Previous research showed that five bacterial isolates were capable of producing the hormone IAA. However, evidence of its potential as a PGPR has not been obtained. Therefore, the aim of this study was to test the effect of inoculation of rhizosphere bacteria on maize plants. The research method uses a quantitative experimental method, namely 15 repetitions for each treatment. Data collection was carried out 1 day after planting (HTS) until the 14th day. Data were analyzed using a 95% confidence student t-test or with a significance level of 5% (a=0.05). This inoculation begins with germinating maize seeds and observing the roots. The germinated maize seeds are then soaked in the bacterial suspension for one hour at room temperature. The results showed that inoculation with isolates P36, P37 and P38 significantly increased the height of maize plants, while isolate P37 significantly increased the number of leaves and root length of corn plants, isolate P38 could significantly increase the number of roots of corn plants.Thus, these results support the evidence that rhizosphere bacteria from frangipani have an effect on increasing the growth of maize plants
Development of primers amplifying DNA barcoding genes matK and rbcL of Legundi (Vitex trifolia) Hartanto, Nadhifah Riski; Sari , Banuwati Kartika; Rahayu, Triasuti; Munawaroh , Rima; Fawwaz , Muammar; Purwono , Bambang; Zulperi, Dzarifah; Prayitno, Trio Ageng; Sidiq, Yasir
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1497

Abstract

Legundi (Vitex trifolia) is one of the crucial ethno-pharmacological plants. However, the genetic exploration of these plants in Indonesia remains limited. Moreover, a set of primers can be an initial important step to explore the genetics of V. trifolia as well as to molecularly identify the species of Legundi. This study aimed to develop the two pairs of primers of maturase-K (matK) and ribulose bisphosphate carboxylase large subunit (rbcL) genes. This type of research is exploratory research using a quantitative approach. The research sampling are the leaves of the V. trifolia species, which were collected from Makassar, Indonesia, and the purposive sampling method. The data were obtained through DNA barcoding, and the data were analyzed using bioinformatics analysis. This study found two pairs of developed primers, matK and rbcL, successfully amplified both matK and rbcL target genes of V. trifolia. The newly developed species-specific primers successfully amplified the matK and rbcL genes of V. trifolia, and sequence analysis revealed high similarity values in BLAST and BOLD databases ranging from 99.2 to 100%, with PCR amplification of the matK marker showing particularly high DNA concentration and specificity for species-level identification. This study supports the genetic exploration and identification of useful ethno-pharmacy plants, V. trifolia. The conclusion of the study shows that two primer pairs of maturase-K (matK) and ribulose bisphosphate carboxylase large subunit (rbcL) genes have been developed from V. trifolia plants. This study supports the genetic exploration and identification of ethnopharmaceutical plants of V. trifolia and the role of bioinformatics tools in molecular studies of medicinal plants.
Pelatihan Penyusunan Bahan Ajar Bermutu Berbantuan Artificial Intelligence pada Guru Sekolah Dasar Kota Madiun Parji, Parji; Yuhanna, Wachidatul Linda; Prayitno, Trio Ageng
Jurnal SOLMA Vol. 15 No. 1 (2026)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v15i1.21233

Abstract

Background: Improving the quality of learning in elementary schools depends heavily on teachers' ability to develop high-quality, innovative, and student-centered teaching materials. Observations indicate that many elementary school teachers in Madiun City still face challenges in developing high-quality teaching materials, due to limited time, creativity, and a lack of understanding of the ideal standards for developing such materials. Elementary school teachers in Madiun City require comprehensive training. The purpose of this training is to implement and analyze the effectiveness of a training program for developing high-quality AI-assisted teaching materials that meet the needs of elementary school teachers. Method: This community service program is implemented by UNIPMA lecturers. The target participants are 50 elementary school teachers in Madiun City. This community service program uses a two-month participatory approach through four stages: outreach, training in developing teaching materials, training in using AI, and monitoring and evaluation. Results: The data showed that 96% of teachers understood the concept of developing high-quality teaching materials, 94% could use AI applications to create digital textbooks, and 92% expressed satisfaction with the community service team's service. Conclusions: The implementation of AI-assisted training to develop high-quality teaching materials was effective in improving teachers' competence while also encouraging more creative, adaptive learning aligned with the demands of 21st-century education.
Pemanfaatan Jamur Metarhizium anisopliae sebagai Pengendalian Hayati Larva Nyamuk Culex sp. trio ageng prayitno; dwi wahyuni
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 2 No 1 (2015): Jurnal Biologi dan Pembelajarannya
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v2i1.325

Abstract

Penggunaan insektisida kimia yang berlebihan sejauh ini banyak menimbulkan dampak negatif bagi lingkungan. Salah satu solusi pengendalian hayati larva nyamuk Culex sp. ialah menggunakan jamur Metarhizium anisopliae yang ramah lingkungan. Pengendalian hayati merupakan salah satu upaya pemberantasan hama dengan memanfaatkan mikroorganisme atau tumbuhan. Tujuan penelitian ini adalah untuk menganalisis LC50 dan LC90 larva instar III nyamuk Culex sp. yang diperlakukan dengan beberapa macam serial konsentrasi jamur Metarhizium anisopliae. Penelitian ini merupakan jenis penelitian laboratoris. Variabel bebas pada penelitian adalah beberapa macam serial konsentrasi jamur Metarhizium anisopliae, sedangkan variabel terikatnya ialah mortalitas larva instar III nyamuk Culex sp.. Data hasil penelitian ini adalah mortalitas larva instar III nyamuk Culex sp. yang diamati selama 48 jam, selanjutnya data tersebut diuji dengan menggunakan analisis Probit. Hasil penelitian menunjukkan bahwa LC50 dan LC90 larva instar III nyamuk Culex sp. yang diperlakukan dengan beberapa macam serial konsentrasi jamur Metarhizium anisopliae berturut-turut adalah 8,782% dan 14,674%.
Efektivitas Pembelajaran Science Environment Technology and Society pada Materi Sistem Kardiovaskular di Tingkat Sarjana Nuril Hidayati; Trio Ageng Prayitno
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 9 No 1 (2022): APRIL 2022
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v9i1.18037

Abstract

The weakness of online learning in human anatomy and physiology courses is that the expected final ability cannot be achieved which has an impact on the quality of learning that is less and less than optimal output. There needs to be a learning process in which there is the use of integrated learning media, innovative learning approaches, one of which is the approach of science, environment, technology, and society. The purpose of this study was to determine the effect of learning SETS integrated with learning media on students' critical thinking skills. The research method used a quasi-experimental design with a pretest-posttest non-equivalent control group design. The results of the study showed that there was an effect of learning SETS integrated learning media on students' thinking skills with a significance value of anaova test of 0.00. The conclusion of this study is that there is a positive influence between the use of SETS learning integrated with learning media on students' critical thinking skills.
Pengaruh Bahan Ajar Mikrobiologi Multimedia Terintegrasi STEM (M-STEM) terhadap Kemampuan Berpikir Kritis dan Komunikasi Mahasiswa Trio Ageng Prayitno; Nuril Hidayati
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 10 No 1 (2023): APRIL 2023
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v10i1.20089

Abstract

The use of multimedia microbiology teaching materials integrated with STEM to empower students' critical thinking and communication skills has not been carried out optimally. The aim of the study was to determine the effect of STEM (M-STEM) integrated multimedia microbiology teaching materials on students' critical thinking and communication skills. The study method is quasi-experimental through a non-equivalent control group design pretest-posttest research design. The independent variable of the study is M-STEM microbiology teaching materials, and the dependent variable of the study is students' critical thinking and communication skills. The research sample was students from the Biology Education Study Program at IKIP Budi Utomo who were taking a Microbiology course. Class A is the research experimental class and class B is the control class. The research instrument was a multiple-choice type test for pretest and posttest questions. The research data are pre-test and post-test scores from the experimental class and control class which will be tested for prerequisites (normality and homogeneity) first and then will be tested with the ANAKOVA test with SPSS. The results of this study indicate that there is an effect of STEM (M-STEM) integrated multimedia microbiology teaching materials on students' critical thinking skills (sig<0.05) and student communication (sig<0.05). The research concludes that multimedia-integrated STEM (M-STEM) microbiology teaching materials have a positive effect on students' critical thinking skills and communication.
Pengembangan Buku Ajar Biologi Berbasis STEM education-PjBL pada Materi Plantae Trio Ageng Prayitno; Nuril Hidayati; Noventianus Umbu Ranja Uma
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 11 No 1 (2024): APRIL 2024
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v11i1.23022

Abstract

STEM education (Science, Technology, Engineering, and Mathematics) based textbooks and Project Based Learning (PjBL) learning models have never been developed by school educators. STEM education-PjBL can provide meaningful learning for students in understanding concepts and exploring through project activities. The aim of the research is to produce a biology textbook based on STEM education-PjBL on Plantae material that meets valid criteria. This type of research is development research and uses the Thiagarajan 4D development model consisting of define, design, develop, and disseminate. However, this research is limited to the develop stage. The research instruments used were a needs analysis questionnaire, a material expert validation questionnaire, and a learning media expert validation questionnaire. The results of the research show that the STEM education-PjBL-based biology textbook on Plantae material is declared very valid and appropriate in terms of material (91%) and learning media (99%). This STEM education-PjBL based biology textbook can be recommended as a learning resource in biology learning and a reference for teachers and other researchers if they want to develop a book by integrating learning approaches and models where the learning approach or model is integrated with the subject matter.
Pengembangan E-LKPD Interaktif Berbasis Games Berbantuan Website Wizer.me pada Materi Sistem Pencernaan Manusia Trio Ageng Prayitno Trio; Nuril Hidayati; Firda Eka Jauharoh
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 11 No 2 (2024): Oktober 2024
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v11i2.23933

Abstract

Interactive e-LKPD based on games assisted by the Wizer.me website has never been developed by educators in schools. Interactive e-LKPD based on games assisted by the Wizer.me website is thought to increase students' motivation to learn. The purpose of the study was to produce interactive e-LKPD based on games assisted by the Wizer.me website on the human digestive system material. The type of research is development research with the Thiagarajan development model consisting of define, design, develop, and disseminate. However, this study is limited to the develop stage. The research instruments are a needs analysis questionnaire, an evaluation expert validation questionnaire, and a learning media expert validation questionnaire. Interactive e-LKPD based on games assisted by the Wizer.me website on the human digestive system material is declared very valid and feasible in terms of evaluation questions (98%) and learning media (97%). Interactive e-LKPD based on games assisted by the Wizer.me website can be recommended as one of the tools to develop student skills, especially Generation Z where teachers can create more interesting and dynamic learning experiences through the use of technology and digital tools.
Pengembangan Game Edukasi Kahoot Berbantuan Augmented Reality (AR) Sketchfab untuk Meningkatkan Minat dan Motivasi Belajar Siswa Eka Astuti; Trio Ageng Prayitno; Nuril Hidayati
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 12 No 2 (2025): OKTOBER 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v12i2.26195

Abstract

Penelitian ini bertujuan untuk mengembangkan game edukasi Kahoot berbantuan Augmented Reality (AR) Sketchfab untuk meningkatkan minat dan motivasi belajar siswa pada materi sistem pernapasan manusia di kelas VIII SMP. Penelitian ini menggunakan metode Research and Development (R&D) dengan model pengembangan ADDIE yang meliputi tahapan analisis, desain, pengembangan, implementasi, dan evaluasi. Instrumen penelitian terdiri dari wawancara, angket validasi ahli materi dan evaluasi, angket kepraktisan, kuesioner, serta pretest dan posttest. Data dianalisis secara deskriptif kualitatif dan deskriptif kuantitatif dengan bantuan SPSS. Hasil penelitian menunjukkan bahwa game edukasi Kahoot berbantuan AR Sketchfab memenuhi kriteria valid dan layak digunakan dari aspek materi (93,68), aspek evaluasi Pendidikan (94,67), dan uji coba kepraktisan (91,2%). Nilai signifikansi adalah kurang dari 0,001, jauh di bawah batas signifikansi 0,05 artinya terdapat perbedaan yang sangat signifikan antara skor pretest dan posttest, serta efektif dalam meningkatkan minat (91,05%) dan motivasi (89,90%) belajar siswa. Dengan demikian, media ini dapat menjadi solusi inovatif dalam pembelajaran IPA untuk menciptakan suasana belajar yang lebih interaktif dan menarik.
Pengaruh Kombinasi Ekstrak Daun Ocimum basilicum dan Tithonia diversifolia terhadap Mortalitas Wereng Coklat (Nilapavarta lugens) pada Tanaman Capsicum frutescens Nila Nuzilatul Fradiana; Trio Ageng Prayitno
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 12 No 2 (2025): OKTOBER 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v12i2.26264

Abstract

Diperlukan alternatif penanggulangan hama tanaman dengan menggunakan pestisida nabati yang bersifat ramah lingkungan dan dapat di produksi secara lokal.Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi ekstrak daun Ocimum basilicum dan Tithonia diversifolia terhadap mortalitas (Nilaparvata lugens) pada (Capsicum frutescens). Penelitian dilaksanakan menggunakan Rancangan Acak Lengkap (RAL) dengan 9 konsentrasi perlakuan (5%–45%) dan satu kontrol, masing-masing diulang sebanyak tiga kali. Parameter yang diamati adalah persentase mortalitas wereng setelah 72 jam aplikasi. Data dianalisis menggunakan uji ANOVA, dilanjutkan dengan uji LSD pada taraf 5%. Hasil menunjukkan bahwa kombinasi ekstrak ekstrak O. basilicum dan T. diversifolia memberikan pengaruh yang signifikan terhadap mortalitas wereng coklat (p < 0,05). Mortalitas tertinggi tercatat pada konsentrasi 45%, yaitu sebesar 90%, yang berbeda nyata dibandingkan dengan konsentrasi lebih rendah.. Penelitian ini menunjukkan bahwa kombinasi ekstrak O. basilicum dan T. diversifolia berpotensi sebagai insektisida nabati yang efektif dan ramah lingkungan dalam pengendalian hama wereng coklat.  
Co-Authors Ade Imas Rismayati Ahmad Ali Amang Fathurrohman Ardian Anjar Pangestuti As'ad Syamsul Arifin Cicilia Novi Primiani Cicilia Novi Primiani Dewi, Aryani Kadarwati Dian Fitri Argarini, Dian Fitri Diana Vivanti Sigit Diyah Ayu Widyaningrum Donny Widianto Dwi Budiono Dwi Wahyuni Dyah Ayu Sulistyaning Cipta Egista Dinka Eka Astuti Eka Suhardi Elfi Anis Saati Endik Deni Nugroho Era Dewi Kartika Fadilah, Fari Fari Fadilah Farizha Irmawati Firda Eka Jauharoh Firda Setiowati Hartanto, Nadhifah Riski Herlambang, Alexander Hermawati, Farah Muthi Ibnu Mujib Iis Nur Asyiah Ilmi Zajuli Ichsan Insani, Qori Tsaniyah Ainun Iriani, Enin Jauharoh, Firda Eka Jazuli Jazuli Khoirun Nisa Khusnia Agustin Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kistantia Elok Mumpuni Listy Hasti Mandiri M, Mukarom Mandiri, Listy Hasti Matrani Mieke Miarsyah Mika Ambarawati, Mika MISTIANAH, MISTIANAH Mohammad Arfi Setiawan Muammar Fawwaz Nikmatul Iza Nikmatul Iza Nila Kartika Sari Nila Nuzilatul Fradiana Noventianus Umbu Ranja Uma Novia El Savada Misdaligo Nur Fadli Hazhar Fachrial Nur Lina Safitri NUR LINA SAFITRI Nur'aini, Afifah Diyah Nurcholis Sunuyeko, Nurcholis Nuril Hidayati Nuril Hidayati Nuril Hidayati Nuril Hidayati Nuril Hidayati Nuril Hidayati NURIL HIDAYATI Nuril Hidayati Nuril Hidayati Nuril Hidayati NURIL HIDAYATI Nuril Hidayati Nuril Hidayati Nuril Hidayati Parji Parji Pasaribu, Aswin Primadya Anantyarta Pujiati , Pujiati Purwono , Bambang Putri Agustina Putri Agustina Qomariah, Ismi Nurul Rahayu, Triasuti Reza Ardiansyah Rima Munawaroh Rita Istiana Rita Retnowati Rivera, Alice Tonido Riyanto Riyanto Riyanto Riyanto Roisatul Ainiyah Sari , Banuwati Kartika Setiawan, Dwi Candra Suci Putri Lestari Sulifah Aprilya Hariani Susandi Susandi Syarafina Syarafina Titik Wijayanti Triastuti Rahayu Triyandana , Arga Vina Listiawati Wachidatul Linda Yuhanna Wijayanti, Titik Wiwin Pramita Arif Wiwin Pramita Arif Yasir Sidiq Yeni Widiyawati yohamintin, yohamintin Yuskil Mushofi Yuskil Mushofi Zainul Mustofa Zulperi, Dzarifah