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The Abundance of Bacteria Associated with Intertidal Seaweeds Sargassum sp. and Kappaphycus alvarezii in Ekas Bay Muahiddah, Nuri; Cokrowati, Nunik; Affandi, Rangga Idris; Junaidi, Muhammad; Sumsanto, Muhammad; Dwiyanti, Septiana; Asri, Yuliana; Lumbessy, Salnida Yuniarti
Journal of Fish Health Vol. 4 No. 1 (2024): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v4i1.4898

Abstract

This study investigates the abundance and diversity of bacteria associated with two intertidal seaweeds, Sargassum sp. and Kappaphycus alvarezii, in Ekas Bay. Intertidal zones are dynamic environments where seaweeds and their associated microbial communities play crucial roles in ecological processes. Seaweeds host a variety of bacteria that contribute to nutrient cycling, seaweed health, and overall ecosystem function. The primary variable measured in this study was the bacterial abundance, expressed as Colony Forming Units per milliliter (CFU/mL). The conclusion of these findings has positive implications for the environmental health around the floating raft, base stakes, Sargassum sp. and Kappaphycus alverazii. The absence of harmful bacteria supports the sustainability of aquatic ecosystems and can provide positive benefits for the species inhabiting those areas.
Natural Feed Nitzschia sp. Culture on Laboratory Scale Susianti, Lena; Affandi, Rangga Idris
Journal of Fish Health Vol. 4 No. 2 (2024): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v4i2.5515

Abstract

Natural feed is fish feed that has the form of microscopic organisms found in waters. Commonly used natural feeds are phytoplankton, zooplankton, protozoa, microcrustaceans, microscopic invertebrates, and others. The need for natural feed is growing in line with the increasing fisheries cultivation efforts. Phytoplankton has a very important role in waters, its ecological function as a primary producer and the beginning of the food chain causes phytoplankton to often be used as a measure of the fertility of waters. One of the phytoplankton that is abundant in waters because of its ability to survive even in extreme conditions and has a fat content is Nitzschia sp., a member of the Bacillariophyceae class. Nitzschia sp. plays an important role as a primary producer and is widely used as natural feed for marine organism larvae such as crustaceans, bivalves, and fish. The purpose of this study was to determine the laboratory-scale Nitzschia sp. natural feed culture technique to support cultivation activities. This research was conducted from March to April 2024 at the Marine Aquaculture Center (BPBL) Lombok, West Sekotong, West Lombok Regency, West Nusa Tenggara. The research methods used are descriptive methods and field observations (surveys). The results that the laboratory scale Nitzschia sp. natural feed culture technique includes equipment sterilization activities, culture preparation, seed distribution, density calculation and harvesting. Factors that affect the growth of Nitzschia sp. are culture media that are free from contamination, initial stocking density, light intensity of 2000-5000 Lux, water quality (pH, salinity, and temperature).
Effectiveness of Octopus (Octopus sp.) Ink Exctract on the Growth of Catfish (Clarias sp.) Infected with Aeromonas hydrophila Saputra, Marta Ade; Scabra, Andre Rachmat; Affandi, Rangga Idris
Journal of Fish Health Vol. 5 No. 1 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i1.6102

Abstract

One material that can be used to improve feed quality is octopus ink. Octopus ink can serve as an alternative nutrient source to optimize catfish growth. Ink produced by cephalopods such as squid, octopus, and cuttlefish contains various components, including fat, protein, ash, and carbohydrates. The aim of this research is to determine the effect of administering octopus ink extract (Octopus sp.) on the growth of catfish (Clarias sp.) infected with Aeromonas hydrophila bacteria. The method used in this research was experimental, involving five treatments with three replications: treatment 1 (Control/P1), treatment 2/P2 (40 ml/kg), treatment 3/P3 (80 ml/kg), treatment 4/P4 (120 ml/kg), and treatment 5/P5 (160 ml/kg). The research steps included octopus ink extraction, a Lethal Concentration 50 (LC50) test, preparation of tools and materials, supplementation of octopus ink extract in feed, application of test treatments, challenge tests with Aeromonas hydrophila, and maintenance. The results of this research showed that treatment 5 (160 ml/kg) yielded the best outcomes in growth parameters, including an absolute weight of 1.26 grams, a specific growth rate of 0.57%/day, a feed convertion ratio (FCR) of 1.66, and a survival rate of 90%.
Efficiency of Using Kappa-Carrageenan as an Immunostimulant Agent in the Non-Specific Immune System of Vannamei (Litopenaeus vannamei) Infected with AHPND (Acute Hepatopancreatic Necrosis Disease) Azhar, Fariq; Scabra, Andre Rachmat; Muahiddah, Nuri; Affandi, Rangga Idris
Journal of Fish Health Vol. 5 No. 1 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i1.6358

Abstract

Vibrio parahaemolyticus is one of the bacteria that can attack whiteleg shrimp and cause Acute Hepatopancreatic Necrosis Disease (AHPND) or Early Mortality Syndrome (EMS) in shrimp. One alternative that can be used is the use of seaweed-based immunostimulants, namely k-carrageenan. The purpose of this study was to evaluate the effectiveness of k-carrageenan mixed in feed with different doses on increasing the immune system of whiteleg shrimp infected with AHPND. This study was conducted for 60 days with an experimental method in the form of a Completely Randomized Design (CRD) consisting of 5 treatments and 3 replications, namely, P1 (Positive Control): Commercial Feed + Vibrio parahaemolyticus Infection; P2 (Negative Control): Commercial Feed + 0.9% NaCl Infection; P3: Commercial Feed + Kappa-carrageenan 8 g / kg + Vibrio parahaemolyticus Infection; P4: Commercial Feed + Kappa-carrageenan 10 g/kg + Vibrio parahaemolyticus Infection; P5: Commercial Feed + Kappa-carrageenan 12 g/kg + Vibrio parahaemolyticus Infection. The results of this study indicate that the addition of 12 g/kg of k-carrageenan to the feed affects the survival rate and immune system of whiteleg shrimp infected with Vibrio parahaemolyticus.
Optimization of Hatchery Techniques to Enhance Larval Survival and Business Feasibility of Guppy (Poecilia reticulata) Setyono, Bagus Dwi Hari; Ula, Nurtia Nikmatul; Affandi, Rangga Idris
Journal of Fish Health Vol. 5 No. 2 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i2.7093

Abstract

Optimizing hatchery techniques for guppy (Poecilia reticulata) production is essential for improving reproductive efficiency and economic viability in small-scale ornamental aquaculture. This study evaluated the effectiveness of a simplified spawning system using sterilized aquaria (40×60×40 cm) with aeration and aquatic vegetation to create a semi-natural environment. Broodstock selection based on gonadal maturity and a male-to-female ratio of 2:4 resulted in high spawning success, with courtship behavior observed by day three. A staged feeding strategy—infusoria and egg yolk (days 1–3), Artemia nauplii (days 4–10), and Tubifex (days 11–21)—supported consistent larval growth from 0.5 cm to 1.6 cm over three weeks. Larval survival reached 91.1%, with 162 of 168 fry surviving by day 21. Stable water quality parameters (24–26°C, 7.6–7.9 mg/L dissolved oxygen, pH 6.7–7.1) were maintained through regular siphoning and water renewal. Economic analysis indicated system feasibility, with a production cost of IDR 1,369,250, revenue of IDR 1,620,000, and net profit of IDR 250,750. The benefit-cost ratio (R/C) was 1.18, the break-even point was achieved at 136.93 fry, and the payback period was 17.7 months. These findings highlight the biological and financial viability of low-cost guppy hatchery systems suitable for rural aquaculture settings. Future research should focus on scaling potential, feed alternatives, and digital monitoring to improve system sustainability and profitability.
Climate-Resilient Biofloc Aquaculture: Energy Efficiency, System Robustness, and Operational Adaptations under Changing Environmental Conditions (2020–2025) Setyono, Bagus Dwi Hari; Abidin, Zaenal; Affandi, Rangga Idris
Journal of Fish Health Vol. 5 No. 4 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i4.8259

Abstract

Climate change poses a significant threat to the sustainability of global aquaculture, including biofloc-based aquaculture systems (BFT). While BFT improves nutrient use efficiency and reduces water consumption, energy efficiency remains a key challenge, especially in terms of energy intensity and oxygen transfer (OTE/SOTE). This study conducts a systematic literature review (SLR) using the PRISMA 2020 method, focusing on energy efficiency in tilapia cultivation with BFT and adaptation strategies to climate variability. Key findings emphasize the importance of aeration, design optimization, and C/N management in minimizing energy consumption without compromising production. Additionally, integrating technologies like IoT/AI for adaptive DO control shows great potential in reducing energy intensity. This research contributes a systematic framework that links energy metrics with aeration design and operational adaptation strategies, offering guidance for developing more efficient and climate-resilient BFT systems. The study also recommends implementing renewable energy and smart water quality monitoring technologies to enhance aquaculture sustainability.
Evaluation of Alginate Use as an Immunostimulant in Litopenaeus vannamei Shrimp Aquaculture (Review) Muahiddah, Nuri; Effendi, Irzal; Supriyono, Eddy; Affandi, Rangga Idris
Journal of Fish Health Vol. 5 No. 4 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i4.8402

Abstract

The Pacific white shrimp (Litopenaeus vannamei) is one of the most important species in global aquaculture but remains highly vulnerable to bacterial, viral, and environmental stressors. Conventional reliance on antibiotics and chemicals has raised concerns over resistance and sustainability, underscoring the need for eco-friendly alternatives. Alginate, a natural polysaccharide extracted from brown seaweeds such as Sargassum, has emerged as a promising immunostimulant and functional feed additive. Experimental studies show that alginate supplementation enhances non-specific immune responses, including total haemocyte count, phenoloxidase, superoxide dismutase, phagocytic activity, and total plasma protein. At the molecular level, alginate upregulates immune-related genes (e.g., Toll, LGBP, proPO), resulting in higher resistance against Vibrio spp. and White Spot Syndrome Virus (WSSV). In addition, low molecular weight sodium alginate improves antioxidant activity and stress tolerance under cadmium exposure, while dietary supplementation supports growth performance and feed utilization. From a sustainability perspective, converting Sargassum biomass into alginate aligns with circular economy principles by transforming an abundant, underutilized seaweed into high-value aquafeed additives. This reduces dependency on antibiotics and synthetic chemicals while generating added value for coastal communities. Overall, alginate represents a multifunctional and cost-effective strategy to strengthen shrimp immunity, enhance resilience to pathogens and stressors, and promote environmentally responsible aquaculture production.
Feed production techniques using local raw materials for mud crab farmers in Madak Belek Hamlet, Sekotong District Diamahesa, Wastu Ayu; Lumbessy, Salnida Yuniarti; Azhar, Fariq; Abidin, Zaenal; Badrun, Badrun; Affandi, Rangga Idris; Sumsanto, Muhammad; Diniariwisan, Damai; Rahmadani, Thoy Batun Citra; Setyono, Bagus Dwi Hari
Penamas: Journal of Community Service Vol. 5 No. 4 (2025): Penamas: Journal of Community Service
Publisher : Nur Science Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53088/penamas.v5i4.2284

Abstract

This community service activity focused on developing feed production techniques using local raw materials for mud crab farmers in Madak Belek Hamlet, Sekotong District. The main challenge faced by farmers is the high cost of commercial feed, which reduces profit margins. In fact, the area is rich in underutilized nutrient-rich local resources such as trash fish and seafood processing by-products. A participatory approach was applied, including observation, socialization, demonstration, hands-on training, and evaluation. The training emphasized feed formulation and the application of 5% wheat gluten as a binder, which had been proven in preliminary studies to enhance feed stability in water. The results showed an improvement in participants’ knowledge and skills in processing local ingredients into quality feed. The expected impacts include reducing dependence on commercial feed, lowering production costs, and improving the efficiency and sustainability of mud crab farming. For further optimization, feed performance testing on crab growth and economic feasibility analysis are required.
SOSIALISASI DAN PENDAMPINGAN TEKNIS KARANTINA BENIH LOBSTER PASIR Panulirus homarus DI KUB SEGARE LAUQ DUSUN UJUNG BETOK JEROWARU LOMBOK TIMUR Asri, Yuliana; Hizbulloh, Lalu; Diniariwisan, Damai; Sumsanto, Muhammad; Affandi, Rangga Idris; Dwiyanti, Septiana; Farizi, Muh. Rizawan
Jurnal Pepadu Vol 6 No 3 (2025): Jurnal Pepadu
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v6i3.8474

Abstract

Lobster pasir (Panulirus homarus) salah satu komoditas perikanan dengan nilai ekonomi tinggi yang memiliki permintaan besar di pasar lokal maupun internasional. Peningkatan kebutuhan tersebut menjadikan budidaya lobster pasir sebagai peluang usaha yang potensial dan menguntungkan bagi masyarakat pesisir di Dusun Ujung Betok. Dusun Ujung Betok merupakan wilayah pesisir yang memiliki potensi sumber daya laut dan wilayah yang ditetapkan sebagai kawasan budidaya lobster pasir di NTB. Masyarakat nelayannya memiliki sebuah Kelompok Usaha Bersama (KUB) yang bernama Segare Lauq yang bergerak dibidang perikanan dan hasil budidaya laut terutama lobster. Kendala utama yang dihadapi oleh pembudidaya adalah kondisi benih tidak 100% sehat dan siap dibudidayakan. Hampir 50% benih yang didatangkan dari luar atau ditangkap dari alam, dalam satu hingga dua hari pemeliharaan akan mengalami kematian. Permasalahan tersebut dapat diatasi dengan cara penanganan (karantina) benih di dalam sebuah wadah yang disebut wadah karantina sebelum ditebar. Tim pengabdian melakukan sosialisasi dan pendampingan teknis dalam pembuatan wadah karantina dan pemanfaatannya. Wadah karantina didisain berbentuk tabung dengan ketinggian 1,5 m, diameter 1 m dan ukuran mata jaring 1 inch. Melalui penyediaan wadah karantina diharapkan mampu mendukung kegiatan budidaya lobster untuk menghasilkan benih yang lebih sehat, seragam dan siap dibudidayakan. Selain itu wadah karantina berfungsi untuk menampung lobster yang baru dipanen, sehingga dapat memudahkan pembudidaya dalam sortasi dan  mengurangi stress pada lobster yang siap kirim. Kegiatan program pengabdian melalui skema Pengabdian Karya Dosen yang Dimanfaatkan Masyarakat ini juga diharapkan bisa meningkatkan kinerja yaitu pada IKU 5, hasil kerja dosen dapat digunakan dan dimanfaatkan oleh masyarakat.
IMPROVING THE HEALTH OF VANNAMEI SHRIMP (Penaeus Vanname) MAINTAINED IN LOW-SALINITY MEDIA WITH POTASSIUM MINERAL SUPPLEMENTATION Scabra, Andre Rachmat; Azhar, Fariq; Affandi, Rangga Idris; Iskandar, M Sopiyan
Jurnal Perikanan Unram Vol 15 No 6 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i6.1056

Abstract

Penambahan berbagai jenis mineral pada kegiatan budidaya udang vannamei media air tawar diketahui menghasilkan pertumbuhan udang vannamei yang lebih tinggi. Namun demikian, pertumbuhan udang vannamei pada habitat aslinya, yaitu air laut, masih menghasilkan pertumbuhan yang masih lebih tinggi. Penelitian ini bertujuan untuk meningkatkan pertumbuhan dan menganalisa respon imun udang vannamei L. vannamei yang dipelihara pada media air tawar dengan penambahan mineral kalium dan diuji tantang dengan bakteri vibrio parahaemoliticus. Informasi tentang respon imun tersebut menjadi dasar pengembangan formulasi mineral kalium yang dapat mendukung kesehatan udang sehingga pertumbuhannya dapat berjalan dengan maksimal. Metode penelitian yang digunakan adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan dan 3 ulangan (P1 : Pemeliharaan media air laut (30 ppt); P2 : Suplementasi Kalium 0 mg/L + injeksi vibrio sp; P3 : Suplementasi Kalium 10 mg/L + injeksi vibrio sp; P4 : Suplementasi Kalium 20 mg/L + injeksi vibrio sp; P5 : Suplementasi Kalium 30 mg/L + injeksi vibrio sp.). Berdasarkan penelitian yang telah dilakukan, dapat disimpulkan bahwa dosis penambahan kalium terbaik terjadi pada perlakuan P5 yaitu sebesar Kalium 45 mg/L. Pada dosis tersebut, perlakuan memberikan pengaruh nyata terhadap nilai SR, pertumbuhan, efisiensi pakan, dan sistem imun (THC, DHC, AF). Pada P5, nilai Survival Rate 50%, Pertumbuhan bobot mutlak  34 g, Efisiensi pakan 57%, THC 28 × 106, AF 58 %.
Co-Authors A Rahman Abd Saddam Mujib Abidin, Z Abidin, Zaenal Adriana Monica Sahidu Agustin, Pradita Ayu Aini, Kuratul Andi Maria Ulfa Andre Rachmat Scabra Angga, Prayogi Dwina Anggraini, Irika Devi Annisa Azzahra, Annisa ANNISA, KARIMA NUR Anton Anton Arning Wilujeng Ekawati Asri, Yuliana Atmawinata, Lalu Mukhtar Auliah, Jami'atul Awan Dermawan Ayu Diamahesa, Wastu Badrun Badrun Baehaqi BAGUS DWI HARI SETYONO Bagus Dwi Hari Setyono Bagus Dwi Hari Setyono Bagus Dwi Hari Setyono Baihaqi, Lalu Wirahadi Al Baiq Triska Saomadia BETARI ATHIYAH IRAWATI Chen, Peter Damai Diniariwisan Damai Diniariwisan Dewi Putri Lestari Dewi Putri Lestari, Dewi Putri Diamahesa, Wastu Ayu Diniariwisan, Damai Dwi Budi Santoso Dwiyanti, Septiana Eddy Supriyono Fariq Azhar Farizi, Muh. Rizawan Hafizah, Imroatul Hizbulloh, Lalu I Gusti Lanang Parta Tanaya Irzal Effendi Iskandar, M Sopiyan Isnaini, Siti Kaswadi, Hendri Khairul Umam Laily Fitriani Laily Fitriani Mulyani Laily Fitriani Mulyani Lalu Mukhtar Atmawinata Lalu Mukhtar Atmawinata Lalu Wirahadi Al Baihaqi Lumbessy, Salnida Yuniarti M Junaidi, M M. Yogi Riyantama Isjoni Malik, Muhammad Dzakiy Marno, Septhian MAS’UD, NURYADIN IBNU Maudina, Finda Maulana, Rona MOHAMAD FADJAR Mohamad Fadjar Muhammad Amin Muhammad Junaidi Muhammad Junaidi Muhammad Marzuki Muhammad Marzuki Muhammad Sumsanto Mulyani, Laily Fitriani Nanda Diniarti Nunik Cokrowati Nur Rahmawati Nuri Muahiddah OLIVIA NINGSIH Panosa, Alief Erwanda Pebrianti, Ni Luh Mutiara Prapti, Az-Zahra Nandia Purwati, Siva Putri, Anesya Wandining Rahmadani, Thoy Batun Citra Rahmadhani, Thoy Batun Citra Retno Palupi Rhojim Wahyudi Rizky Regina Kawirian RR. Ella Evrita Hestiandari Rusmin Hari Nuryadin Rusmin Nuryadin Sahrul Alim Saomadia, Baiq Triska Sapto Andriyono Saputra, Marta Ade Septiana Dwiyanti Septiana Dwiyanti Septiana Dwiyanti Soenarky, Wiwin Iky Suhardinata Sukartono Sukartono Susianti, Lena Syawalina Fitria Syawalina Fitria Syawalina Hari Fitria Thoy Batun Citra Rahmadani Thoy Batun Citra Rahmadani Thoy Batun Citra Rahmadani Thoy Batun Citra Rahmadani Ula, Nurtia Nikmatul Wiwik Handayani Wiwin Iky Soenarky Wulandari, Witri Yanti, Septiana Dwi Yatin, Nur Yuliana Asri Yuliana Asri Yuliana Asri Yuliana Asri Zaenal Abidin Zaenal Abidin