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Efek Ekstrak Metanol Daun Ketapang (Terminalia catappa L.) Terhadap Kepadatan Kolagen dalam Penyembuhan Luka Bakar Derajat II pada Tikus Putih (Rattus norvegicus) Yulianto, Rangga; Triakoso, Nusdianto; Saputro, Amung Logam; Setiawan, Boedi; Yudhana, Aditya; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.591 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.82-88

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh ekstrak metanol daun ketapang (Terminalia catappa L.) untuk meningkatkan kepadatan serabut kolagen dalam penyembuhan luka bakar derajat II pada tikus putih (Rattus norvegicus). Sebanyak dua puluh ekor tikus putih (Rattus norvegicus) jantan dibagi ke dalam lima kelompok perlakuan yaitu K- luka dengan pemberian basis salep, K+ luka dengan pemberian Bioplacenton®, P1 luka bakar dengan pemberian salep ekstrak daun ketapang 25%, P2 luka bakar dengan pemberian salep ekstrak daun ketapang 50% dan P3 luka bakar dengan pemberian salep ekstrak daun ketapang 100%. Terapi diberikan sehari sekali selama tujuh hari. Hasil data rata-rata jumlah kepadatan serabut kolagen menunjukkan K- 83.57%, K+ 84.52%, P1 90.32%, P2 86.77% dan P3 83.36%. Kelompok P1 dan P2 tidak memiliki perbedaan yang nyata, tetapi berbeda nyata dengan kelompok K+, K- dan P3. Kesimpulan dari penelitian ini ekstrak metanol daun ketapang berpengaruh dalam meningkatkan kepadatan serabut kolagen pada proses penyembuhan luka bakar derajat II.
Pengaruh Kitosan Udang Secara Topikal Terhadap Kepadatan Kolagen dalam Penyembuhan Luka Eksisi pada Tikus Putih Prastika, Dinda Dwi; Setiawan, Boedi; Saputro, Amung Logam; Yudaniayanti, Ira Sari; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (839.412 KB) | DOI: 10.20473/jmv.vol3.iss1.2020.101-107

Abstract

Luka eksisi adalah luka yang permukaan kulit dan lapisan bawahnya akan terpotong sampai kedalaman yang bervariasi dapat terjadi secara sengaja atau tidak sengaja. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh chitosan kulit udang terhadap kepadatan kolagen dalam proses penyembuhan luka eksisi pada tikus putih. Sebanyak dua puluh ekor tikus putih (Rattus norvegicus) jantan dibagi ke dalam lima kelompok perlakuan yaitu K- luka eksisi dengan pemberian basis salep, K+ (luka eksisi+10% povidone iodine), P1 (luka eksisi+salep chitosan kulit udang 1.5%), P2 (luka eksisi+udang salep kulit kitosan 2.5%), dan P3 (luka eksisi+kitosan kulit udang 5%). Hasil perhitungan persentase kepadatan kolagen dianalisis oleh Kruskal-Wallis yang menunjukkan perbedaan signifikan (p<0.05) dan dilanjutkan dengan uji Mann-Whitney U. Kelompok K+, K- dan P1 memiliki perbedaan signifikan dalam kepadatan kolagen pada P2, dan kelompok P3 (p<0.05). Kesimpulan dari penelitian ini kitosan berpengaruh terhadap kepadatan kolagen dengan dosis 5%.
Effectiveness of Clove Flower Extract (Syzygium Aromaticum L.) as Analgesic on Licking Time Reaction in Male Mice with Formalin Induction Puspitasari, Vina; Wahjuni, Retno Sri; Saputro, Amung Logam; Hamid, Iwan Sahrial; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.226-230

Abstract

This research is about testing the effectiveness of clove flower extract (Syzygium aromaticum L.) as an analgesic against the beating time caused by formalin male mice. This research is experimental with the Complete Random Design formula producing four times repetition (N = 4) and divided into five treatment groups (t = 5). Data were analyzed using ANAVA factorial test then followed by Duncan test. All tests are carried out with Product Solutions and Service Statistics (SPSS). The results of research and statistical analysis prove that clove flower extract at a dose of 1% is an effective dose compared to other treatment groups, seen from the amount of time licking male mice  which is smaller than other treatment groups. It can be concluded that the administration of clove flower extract has analgesic effectiveness against the reaction of male mice induced by formalin. The concentration of clove flower extract 1% is the most effective in reducing licking time.
Capillaria spp. in a Reticulated Python (Python reticulatus) in Banyuwangi Reptile Community Dini, Sayyida Kamila; Triakoso, Nusdianto; Saputro, Amung Logam; Yudhana, Aditya
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.119-123

Abstract

Reptiles as exotic pets become more commonly desired by society, one of which is the reticulated python. Pythons kept as pets also have a risk of disease infection including helminthiasis. Worm parasite infects snake by the oral route. Some snake feed including frogs and mice can act as an intermediate host for worm parasites. A male reticulated python was kept by a member of the Banyuwangi reptile community and was presented with healthy and normal eating habits. This case study was to determine the worm parasite in the wild-caught reticulated python kept as an exotic pet. Fresh fecal samples were stored in a container with 70% alcohol added as a preservative. The sample was then evaluated in the laboratory and examined using the native method, floatation method, and simple sedimentation method. As result, under the microscope to be the Capillaria spp. the egg was observed.
Service per Conception, Conception Rate, Calving Rate and Non-Return Rate in Beef Cattle in Kalipuro, Banyuwangi Dinul, Ahmad Rajul; Restiadi, Tjuk Imam; Wibawati, Prima Ayu; Ratnani, Hermin; Saputro, Amung Logam; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.54-61

Abstract

This study aimed to determine the number of reproductive efficiency parameters including service per conception, conception rate, calving rate, and non-return rate for broilers in Kalipuro District, Banyuwangi Regency. The secondary data was collected from the Banyuwangi District Agriculture Office. This research is also intended to dissect factors related to the S/C, CR, CvR, and NRR figures with descriptive analysis, including bio reproduction mastery factors, service factors, and feed factors. The primary data was collected from beef cattle breeders in Kalipuro District, Banyuwangi Regency. In addition, there were IB implementation factors whose data were obtained from questionnaires to inseminators in Kalipuro District, Banyuwangi Regency. The number of S/C broilers in Kalipuro Subdistrict, Banyuwangi Regency was good maintenance, in January-March 2020 of 1.71, 1.99, and 1.74, meanwhile, the number of CR were 58%, 50% 57%, CvR for January-March were 44%, 84%, 53% and NRR for January, February and March were 41%, 49%, and 46%, respectively, were below the standard rate.
Difference in Lairage Time Before Slaughtering on Malondialdehyde (MDA) Levels in Landrace Pig Blood Serum Muhandis, Putri Jauza Aulia; Triakoso, Nusdianto; Saputro, Amung Logam
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.16-20

Abstract

Lairage time before slaughtering a pig can recover the stress caused by transportation from the farm to the abattoir. Stress conditions can overcome the imbalance between Reactive Oxygen Species (ROS) and the ability of the system to be easily detoxified. Malondialdehyde (MDA) is a specific product of lipid peroxidation, it is often used as a biomarker in research. A total of 18 pigs were divided into two groups, pigs with a lairage time of 6-8 hours and a rest period of 12-24 hours, MDA levels were tested using the Thiobarbituric Acid Reactive Substance (TBARS) method and then analyzed. There was a significant difference (p<0.05) between the group lairage time length of 6-8 hours (18.10 ± 11.616) with a rest period of 12-24 hours (6,80 ± 4,852), there was a decrease in stress levels and was marked by a decreased in levels of MDA in pigs that were rested for 12-24 hours before being slaughtered.
The Effectiveness of Time Equilibration Before Freezing in Sapera Goat Spermatozoa After Electric Separating Sperm Saputro, Amung Logam; Prastiya, Ragil Angga; Ulinuha, Meidina Zulva; Widayani, Prasita
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.1-8

Abstract

The purpose of this study was to determine the effectiveness of time equilibration before freezing sapera goat spermatozoa after ESS (Electric Separating Sperm) on motility, viability, membrane integrity, normality and abnormality of spermatozoa. Sapera goat spermatozoa were collected using an artificial vagina and then separated using the ESS method. This study used a completely randomized formula with three treatments, which are the equilibration time of P1 (1 hour), P2 (3 hours), and P3 (4 hours), with six replications. The analysis in this study used the ANOVA test followed by the Duncan test to determine the real difference between treatments. The conclusion of this study showed the highest percentage of motility, viability, membrane integrity, normality of sapera goat spermatozoa at 1-hour treatment, for the lowest percentage of abnormalities at 1-hour treatment. Significantly different results (p <0.05) on the anode and cathode sides in all equilibration time treatments.
Effect of Addition of Green Tea Extract (Camellia sinensis) in Egg Yolk Tris Diluter on Spermatozoa Quality in Bali Cattle (Bos sondaicus) After Freezing Wijayanti, Ani; Suprayogi, Tri Wahyu; Prastiya, Ragil Angga; Hernawati, Tatik; Sardjito, Trilas; Saputro, Amung Logam; Amaliya, Anny; Sulistyowati, Deny
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.66-74

Abstract

This study aimed to determine the effect of adding 96% ethanol extract of green tea (Camellia sinensis) in egg yolk tris diluter on the quality of spermatozoa of Bali cattle (Bos sondaicus) after freezing. The samples were divided into four different treatment groups, which contained semen sample + 13% glycerol + each egg yolk tris diluter + green tea extract (P0) 0 mg/100 ml, (P1) 0,05 mg/100 ml, ( P2) 0,10 mg/100 ml, and (P3) 0,15 mg/100 ml. The variables observed included motility, viability, and spermatozoa abnormalities. Data were analyzed using OneWay ANOVA and continued with Duncan's test (p<0,05). The results showed that the P3 group had motility of 49,00 ± 2,15, viability of 61,92 ± 1,70, and abnormality of 4,24 ± 2,16 significantly compared to the other groups. In conclusion, the P3 group with the addition of 0,15 mg/100 ml of green tea could increase the motility, viability, and abnormal spermatozoa of Bali cattle.
Seroprevalence Brucellosis in Etawah Crossbreed goat in District Siliragung, Banyuwangi Saputro, Amung Logam; Praja, Ratih Novita; Yudhana, Aditya; Mumtazi, Fauzan; Romadhona, Ma'rifatunnisa'; Anastasya, Anastasya; Farhan, Muhammad Riesta
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.138-144

Abstract

Brucellosis is a zoonotic bacterial disease that is endemic in many countries, especially developing countries such as Indonesia. Livestock centers in Indonesia have not been free from Brucellosis disease, which reaches 40% and spread almost throughout Indonesia. This study aimed to detect the presence of Brucellosis antibodies in Etawah Crossbreed at Siliragung District, Banyuwangi using the Rose Bengal Test (RBT) method. The data that will be obtained later was expected to provide benefits in reducing the possibility of Brucellosis spread and can be used as a reference for Brucellosis control programs in goats. The results showed that the RBT examination in the sample population of Etawah Crossbreed in Siliragung District which was taken showed negative results in all samples. The conclusion of this study was that Brucella abortus antibodies were not found in Etawah Crossbreed in Siliragung District, Banyuwangi.
Isolation, Identification and Risk Factors of Staphylococcus aureus Bacteria in Dairy Goat Milk with Subclinical Mastitis in Siliragung District, Banyuwangi Regency Faizah, Ayu Nur; Setiawan, Boedi; Saputro, Amung Logam; Warsito, Sunaryo Hadi; Praja, Ratih Novita; Fikri, Faisal
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.48012

Abstract

Mastitis is an inflammation of the mammary glands that can cause a sharp reduction in milk production and income for farmers. Subclinical mastitis is mastitis without clinical symptoms but can result in a decrease in the quantity and quality of milk production. One of the main pathogens that cause mastitis namely Staphyloccocus aureus. S. aureus become the biggest problem of mastitis because the cure rate is classified as very low. The risk factors for the occurrence of mastitis, both clinical and subclinical mastitis, can cause a decrease in milk production caused by several factors, including genetic, feed and environmental factors. This study aims to determine the presence of subclinical mastitis in milk of dairy goats with sufferers in Siliragung District, Banyuwangi Regency. Sample testing was carried out at the SIKIA Instrument Laboratory, Airlangga University. Isolation was carried out using MSA (Mannitol Salt Agar) media for further identification consisting of Gram staining and biochemical tests. Some of the biochemical tests used are the catalase test, the coagulase test and the VP test. The results of isolation, observation of characteristics and identification of bacteria in the research that has been carried out show that 64.51% (40/62) of the samples examined were positively identified S. aureus. Results showed that the risk factors for causing subclinical mastitis included cleaning the cage, the number of lactating goats, washing hands before milking, manure and waste management, weaning age and lactation age.
Co-Authors 'Amiroh, Ade Abdul Samik Agnes Theresia Soelih Estoepangestie Agung, Arya Agustono, Bodhi Akmal, Abid Baihaqi Bahrul Al arif, Mohammad Anam Amalia Sofi Ramadanti Amaliya, Anny Amborika, Nadia Anastasya, Anastasya Ani Wijayanti Annerda Yurika Aras, Danish Wildan Ashari, Lukman Ashilla Afandik, Nadya Atikah Madarina Hisyam, Mirza Azmi, Hana Ardelia Boedi Setiawan Cahya, Rizka Wulan Dilla Chelsea Aziizahrani Santoso Dini, Sayyida Kamila Dinul, Ahmad Rajul Diwanty, Rafif Fazila Diyanah, Khuliyah Candaraning Erma Safitri ERMA SAFITRI Evelyn Zalfa Winni Kusuma Faisal Fikri Faizah, Ayu Nur Farhan, Muhammad Riesta Fitriani Sultan Habib, Mohammad Nabil El Haditanojo, Wiyanto Hamid, Iwan Sharial Hani Plumeriastuti Hermin Ratnani Hidayatuh Sholihah, Alfi Imam Mustofa IMAM MUSTOFA iqbal dwi warsito Ira Sari Yudaniayanti Ivanalee, Angela Swasti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Jihan Annisa Kana Maulida Khafsah, Khafsah Lailiyah, Fahlevi Lake, Werenfridus Kono Lestari, Dewi Sri Lita Rakhma Yustinasari Mafruchati, Maslichah Marshanda, Nawang Brilliant Masyitoh, Herlina Maxdhameta, Styuderil Imaniro Milladiya Khuriyah Zahra, Reyza Mirni Lamid Mu'afi, Muhammad Firdaus Muhammad Gufron Muhammad Ivan Maulana Muhammad Thohawi Elziyad Purnama Muhandis, Putri Jauza Aulia Mukti, Ibrahim Mumtazi, Fauzan Murjianingsih, Fitriani Nove Hidajati Nurina Naqsmagita, Salsabilla Nusdianto Triakoso Pahlevy, Jolly Rifqi Pasha, Zehansyah Algifari Poedji Hastutiek Praja, Ratih Novita Prastika, Dinda Dwi Prastiya, Ragil Angga Primarizky, Hardany Pudji Srianto Puspitasari, Vina Putri, Lusi Rosita Sartono Rachmanita, Rona Taufiqul Rahmadhani, Nurmitasari Rimayanti Rofiqi, Mohammad Miftahur Romadhona, Ma'rifatunnisa' Safitri, Ainun Merta Santoso, Kuncoro Puguh Sasi, Samira Musa Satria, Rafif Galih Sekarsari, Dara Ayu Pramesti Setyawan, Firman Shifa Salsabilla Praja Solikhah, Tridiganita Intan Sri Pantja Madyawati Suherni Susilowati Sulaeman, Nur Sa'adah Sulistiyawati, Elis Sulistyowati, Deny Sunarso, Agus Suryanie Sarudji Tatik Hernawati Tito, Muhammad Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Tyasningsih, Wiwiek Ulinuha, Meidina Zulva Umama, Ayu Riza Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wayan Ari wijaya Wibawati, Prima Ayu Widayani, Prasita Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wira Tirta Jaladara Wisudawati, Feby Dwi Yudhana, Aditya Yudianti, Novi Putri Yulianto, Rangga Yunita, Maya Nurwartanti