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Saputro, Amung Logam
Laboratorium Klinik, Departemen Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus-C Unair Jln. Mulyorejo, Surabaya, Jawa Timur, Indonesia, 60115
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary Other

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Isolation, Identification and Risk Factors of Staphylococcus aureus Bacteria in Dairy Goat Milk with Subclinical Mastitis in Siliragung District, Banyuwangi Regency Faizah, Ayu Nur; Setiawan, Boedi; Saputro, Amung Logam; Warsito, Sunaryo Hadi; Praja, Ratih Novita; Fikri, Faisal
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.48012

Abstract

Mastitis is an inflammation of the mammary glands that can cause a sharp reduction in milk production and income for farmers. Subclinical mastitis is mastitis without clinical symptoms but can result in a decrease in the quantity and quality of milk production. One of the main pathogens that cause mastitis namely Staphyloccocus aureus. S. aureus become the biggest problem of mastitis because the cure rate is classified as very low. The risk factors for the occurrence of mastitis, both clinical and subclinical mastitis, can cause a decrease in milk production caused by several factors, including genetic, feed and environmental factors. This study aims to determine the presence of subclinical mastitis in milk of dairy goats with sufferers in Siliragung District, Banyuwangi Regency. Sample testing was carried out at the SIKIA Instrument Laboratory, Airlangga University. Isolation was carried out using MSA (Mannitol Salt Agar) media for further identification consisting of Gram staining and biochemical tests. Some of the biochemical tests used are the catalase test, the coagulase test and the VP test. The results of isolation, observation of characteristics and identification of bacteria in the research that has been carried out show that 64.51% (40/62) of the samples examined were positively identified S. aureus. Results showed that the risk factors for causing subclinical mastitis included cleaning the cage, the number of lactating goats, washing hands before milking, manure and waste management, weaning age and lactation age.
Risk Factors Causing Repeat Breeding Reproductive Disorders in Beef Cattle in Klabang Sub-District, Bondowoso Regency Rofiqi, Mohammad Miftahur; Saputro, Amung Logam; Praja, Ratih Novita
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.48037

Abstract

This study aims to determine the relationship between factors and cases of repeated breeding in beef cattle in Klabang District, Bondowoso Regency. Beef cattle farming in Klabang District, Bondowoso Regency is currently experiencing problems in meeting the demand for beef due to low productivity. These problems occur due to cases of repeated breeding and decreased livestock efficiency and productivity. This case is suspected due to the lack of sanitation from farmers. This descriptive study used 69 cows with a simple random sampling method. Primary data was obtained from direct observation of livestock, followed by interviews with farmers using an in-depth study system. Meanwhile, secondary data was obtained from inseminator records which included the period of cultivation, the farmer's ability to detect the estrous cycle, and the farmer's knowledge of reproduction, housing, feeding, and copulation in beef cattle. The collected data were analyzed using descriptive statistics and then processed using SPSS 22.0. The Chi-Square test (X2) is used to determine the relationship between causative factors and repeated breeding. The results of this study indicate that the incidence of repeated breeding in Klabang District, Bondowoso Regency is 100% of the sample. All factors such as length of breeding, farmer's ability to detect estrous cycles, and farmer's knowledge about reproduction, housing, feed, age of livestock, and copulation in beef cattle correlate with the incidence of repeated breeding.
Optimization of Sustainable Goat Farm Productivity Improvement through Complete Feed Introduction Technology in Kaliploso Village, Banyuwangi Saputro, Amung Logam; Agustono, Bodhi; Prastiya, Ragil Angga; Mumtazi, Fauzan; Tito, Muhammad
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.48248

Abstract

Increasing the level of the community economy through the livestock sector is increasingly in demand. The livestock sector is experiencing an increase in interest in one of them is goat farming. Not much different from the success factor of increasing the productivity of livestock, the success of goat livestock is also influenced by several factors including genetics, feed, and maintenance management. The role of farmers is necessary to achieve the desired level of productivity success. Breeders in the group Banyuwangi Goat Breeder must be prepared to be a breeder who is aware of the mechanism of raising a good right. Therefore, the education of farmers to be related to the way or technique of raising goats is good and right, goat cultivation management in the form of types of goats, goat reproduction, goat health to the manufacture of complete feed that is right must be understood by farmers. The purpose of this activity is to improve farmers ' understanding of the importance of understanding the basic aspects of breeding including genetic, feed, and maintenance management. In addition to aiming to improve farmers ' understanding of goat breeding techniques, this extension is expected to also be a solution in solving economic and social problems, especially in the field of goat farming, and independently farmers can make complete feed independently. However, the sustainability of coaching is expected to improve the quality of farmers in raising livestock and as a mechanism for evaluating the complete feed until the formation of a commercial and viable complete feed. Feed model as a source of livestock nutrition.
Detection Brucella sp. Antibody in Sapera (Saanen í— Peranakan Etawa) Goat using Rose Bengal Test (RBT) Method in Siliragung Subdistrict Banyuwangi Regency Putri, Lusi Rosita Sartono; Lamid, Mirni; Lokapirnasari, Widya Paramita; Saputro, Amung Logam; Wibawati, Prima Ayu; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.49356

Abstract

This study detected the presence of Brucella sp. antibodies in Sapera goats in Siliragung sub-district, Banyuwangi District using serological tests. The screening test used in this study was the Rose Bengal Test (RBT). Brucellosis causes goats to experience abortion and disruptive of reproduction system. The method used in this research is purposive sampling method using the criteria of sapera goats that have never been vaccinated. Using the blood serum of Sapera goats taken at the Siliragung subdistrict farm, Banyuwangi. The results of the study did not show any samples that were considered positive for Brucellosis in the Rose Bengal Test. In this study, no Brucella sp. antibodies were found in Sapera goats in Siliragung sub-district, Banyuwangi District.
Isolation, Identification and Risk Factors of Escherichia coli Bacteria in Dairy Goat's Milk Subclinical Mastitis in Siliragung District, Banyuwangi Regency Lestari, Dewi Sri; Setiawan, Boedi; Saputro, Amung Logam; Mafruchati , Maslichah; Praja , Ratih Novita; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.49401

Abstract

Mastitis is an inflammation of the mammary gland which can cause a decrease in milk production and income for farmers. Subclinical mastitis is mastitis without clinical symptoms but can result in a decrease in the quality of milk production. One of the main pathogens that cause mastitis is Escherichia coli. The risk factors for the occurrence of mastitis, both clinical and subclinical mastitis, can cause a decrease in milk production caused by several factors, including genetic, feed, and environmental factors. This study aims to determine the presence of subclinical mastitis in dairy goat milk in Siliragung District, Banyuwangi Regency. Sample testing was carried out at the SIKIA Instrument Laboratory, Airlangga University. Isolation was carried out using EMBA media (Eosin Methylene Blue Agar) for further identification consisting of Gram staining and biochemical tests. One of the biochemical tests used is the IMViC (Indole Methyl red Voges-Proskauer Citrate Test). The results of isolation, observation of characteristics, and identification of bacteria in the research that was carried out showed that 8% (5/62) of the samples tested were positively identified as Escherichia coli. The results showed that the risk factors for causing subclinical mastitis included cleaning the cage bedding, washing hands before milking, milking out, teat dipping, and medication.
Prevalence and Risk Factors of Gastrointestinal Helminth Infection in Pet Cats in Wonogiri District, Central Java Sekarsari, Dara Ayu Pramesti; Saputro, Amung Logam; Agustono, Bodhi; Yunita, Maya Nurwartanti; Praja, Ratih Novita; Solikhah, Tridiganita Intan
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.49559

Abstract

Gastrointestinal helminth infection is the most common and the easiest infection to infect wild cats or pet cats but is often ignored by the cat owners because sometimes there are no symptoms or a lack of attention from the cat owner to their pet cat. The aim of this study was to estimate the prevalence and risk factors of gastrointestinal helminth infection in pet cats in Wonogiri District, Central Java. A total of 100 cat samples were evaluated using a questionnaire, then fecal microscopic examination using simple native method, sedimentation, and floatation methods. Data obtained from this study were presented descriptively and analyzed using the Chi-Square test. The result showed that 37 out of 100 fecal samples were found positive infected with gastrointestinal helminth including Toxocara cati and Dipylidium caninum or the prevalence of gastrointestinal helminth infection can be calculated at 37%. The result of the analysis of significant risk factors were breed, number of pet cat owners, grooming, deworming, and vaccination.
Inovasi Microchip Sebagai Sarana Optimalisasi Pabrik Cempe Dengan Data Recording Pada Peternakan Kambing Perah Di Banyuwangi Berbasis Aplikasi Saputro, Amung Logam; Agustono, Bodhi; Angga Prastiya, Ragil; Atikah Madarina Hisyam, Mirza; Milladiya Khuriyah Zahra, Reyza; Ashilla Afandik, Nadya; Nurina Naqsmagita, Salsabilla; Hidayatuh Sholihah, Alfi; Gufron, Muhammad
LOSARI: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2024): Desember 2024
Publisher : LOSARI DIGITAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53860/losari.v6i2.385

Abstract

Abstract Pesanggaran Subdistrict in Banyuwangi Regency, East Java, has significant livestock farming potential, with populations of livestock such as beef cattle, goats, and sheep. However, livestock management, particularly in terms of data recording, is still not optimal. This community service program aims to improve livestock management through digital technology by implementing two main programs: SEMAR (Setaun Manak Loro) and DISFAR (Digital Smart Farming). SEMAR focuses on education regarding dairy goat reproduction management, while DISFAR provides digital recording tools in the form of chips/ear tags to monitor estrus periods, mating, birth predictions, and livestock health records. The methods used in this program include counseling, training, evaluation, and cadre development over a 10-month period. The outcomes expected from this program include the publication of articles in reputable community service journals, popular articles in the local media, Radar Banyuwangi, a digital recording guidebook, and tutorial videos on YouTube. The implementation of this program is expected to increase the efficiency and productivity of livestock farming in Pesanggaran through the proper use of digital technology. Abstrak Kecamatan Pesanggaran di Kabupaten Banyuwangi, Jawa Timur, memiliki potensi peternakan yang signifikan dengan populasi ternak seperti sapi potong, kambing, dan domba. Manajemen peternak, khususnya dalam hal pencatatan data (recording), untuk saat ini masih belum optimal. Program pengabdian masyarakat ini bertujuan untuk meningkatkan manajemen ternak melalui teknologi digital dengan mengimplementasikan dua program utama SEMAR (Setahun Manak Loro) dan DISFAR (Digital Smart Farming). SEMAR fokus pada penyuluhan manajemen reproduksi kambing perah, sedangkan DISFAR menyediakan sarana recording digital berupa chip/ear tag untuk memantau masa birahi, kawin, prediksi kelahiran, dan catatan kesehatan ternak. Metode yang digunakan dalam program ini meliputi penyuluhan, pelatihan aplikasi, evaluasi, dan pembinaan kader dalam komunitas peternakan. Hasil dari program ini diharapkan mencakup publikasi artikel di jurnal layanan masyarakat bereputasi, artikel populer di media massa, buku pedoman recording berbasis digital, serta video tutorial di YouTube. Implementasi program ini diharapkan dapat meningkatkan efisiensi dan produktivitas peternakan di Pesanggaran dengan penggunaan teknologi digital yang memadai.
Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) iqbal dwi warsito; Suherni Susilowati; Erma Safitri; Ragil Angga Prastiya; Maya Nurwartanti Yunita; Amung Logam Saputro
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.16-24

Abstract

This study aimed to evaluate the protective effects of α-tocopherol and zinc sulfate on oxidative stress parameters in ovarian granulosa cells of female Wistar rats (Rattus norvegicus) exposed to lead acetate (Pb(C₂H₃O₂)₂). A total of 25 rats were randomly divided into five groups: Control (C), lead exposure only (T0), α-tocopherol + lead (T1), zinc sulfate + lead (T2), and α-tocopherol + zinc sulfate + lead (T3). Treatments were administered orally for 21 days. Superoxide dismutase (SOD) levels were measured spectrophotometrically, and p53 expression was analyzed using immunohistochemistry. The results showed that SOD levels significantly decreased in group T0 compared to the control (p <0.05), while treatment with either α-tocopherol (T1), zinc sulfate (T2), or their combination (T3) significantly improved SOD levels compared to T0. Moreover, p53 expression was markedly elevated in T0, indicating enhanced oxidative stress and potential apoptosis, whereas all antioxidant-treated groups showed reduced p53 expression, with T3 demonstrating values comparable to the control group. In conclusion, the combination of α-tocopherol and zinc sulfate provided a synergistic antioxidant effect, effectively enhancing SOD levels and suppressing p53 expression in granulosa cells of lead-exposed rats. This suggested a promising therapeutic potential of these compounds in mitigating lead-induced ovarian toxicity.
Identifikasi Salmonella sp. pada Susu Segar di Peternakan Sapi Perah Kecamatan Licin Kabupaten Banyuwangi Satria, Rafif Galih; Hamid, Iwan Sahrial; Wibawati, Prima Ayu; Estoepangestie, Agnes Theresia Soelih; Saputro, Amung Logam; Praja, Ratih Novita
Media Kedokteran Hewan Vol. 32 No. 3 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i3.2021.114-118

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui tingkat cemaran bakteri Salmonella sp. pada susu segar dari peternakan sapi perah di kecamatan licin kabupaten banyuwangi. Sampel susu segar di ambil pada april 2021 sebanyak 16 sampel dari KPSP Ijen makmur di kecamatan Licin Kabupaten Banyuwangi. Sampel diambil sebanyak 25 ml dari masing-masing peternak. Hasil yang di dapat setelah melakukan isolasi dan identifikasi menggunakan media Salmonella Shigella Agar (SSA), dan dilanjutkan uji identifikasi menggunakan TSIA dan Urea didapatkan  dari ke 16 sampel semua dinyatakn negatif bakteri Salmonella sp.. Hal ini dapat terjadi karena para peternak sapi perah di Kecamatan licin sudah mulai menyadari pentingnya kebersihan kandang dan sanitasi kandang yang baik sehingga tingkat cemaran bakteri Salmonella sp. dapat dicegah.
The addition of vitamin C in tris–egg yolk extender maintained Sapera goat semen quality in 5° C storage Pahlevy, Jolly Rifqi; Ratnani, Hermin; Restiadi, Tjuk Imam; Fikri, Faisal; Saputro, Amung Logam; Agustono, Bodhi
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.1-8

Abstract

Goats are small ruminants that are reared by the rural community for financial income and nutrition. This study was aimed to determine the effect of vitamin C addition in tris–egg yolk extender on the lifespan of Sapera goat spermatozoa stored in 5o C. Semen was collected from 1.5 years old Sapera male goat. The ejaculates were diluted in Tris-egg yolk (T-EY) extender added with 0, 0.2, 0.3, and 0.4 g vitamin C/mL extender for T0, T1, T2, and T3 groups, respectively. The spermatozoa motility, viability, and morphological abnormality were assessed along with storage at 5° C. The result showed that spermatozoa motility was the highest (p <0.05) in T1 compared to other groups and qualified for AI use for up to 72 hours. In 24 hours storage, the spermatozoa viability was not significantly different (p >0.05) among the groups. The spermatozoa viability in the T1 group was the highest (p <0.05) among the groups along 48-96 hours of storage. The spermatozoa morphological abnormalities of the T1 group was the lowest (p <0.05) compared to other treatment groups in the range of 24-72 hour storage. In the control group (T0), the less than 5% spermatozoa morphological abnormalities (qualified for artificial insemination) were only in the 24 hours storage, while those of the T1 group were up to 72 hours. It could be concluded that the addition of 0.2 g vitamin C/100 mL T-EY extender maintained the quality of Sapera goat semen for 72 hours at 5°C.
Co-Authors Abdul Samik Agustono, Bodhi Amaliya, Anny Anastasya, Anastasya Ani Wijayanti Ashari, Lukman Ashilla Afandik, Nadya Atikah Madarina Hisyam, Mirza Boedi Setiawan Cahya, Rizka Wulan Dini, Sayyida Kamila Dinul, Ahmad Rajul ERMA SAFITRI Erma Safitri Estoepangestie, Agnes Theresia Soelih Faisal Fikri Faizah, Ayu Nur Farhan, Muhammad Riesta Haditanojo, Wiyanto Hamid, Iwan Sharial Hani Plumeriastuti Hermin Ratnani Hidajati, Nove Hidayatuh Sholihah, Alfi IMAM MUSTOFA Imam Mustofa iqbal dwi warsito Ira Sari Yudaniayanti Ivanalee, Angela Swasti Iwan Sahrial Hamid Iwan Sahrial Hamid Khafsah, Khafsah Lailiyah, Fahlevi Lake, Werenfridus Kono Lestari, Dewi Sri Luqman, Epy Muhammad Mafruchati, Maslichah Masyitoh, Herlina Milladiya Khuriyah Zahra, Reyza Mirni Lamid Muhammad Gufron Muhammad Thohawi Elziyad Purnama Muhandis, Putri Jauza Aulia Mumtazi, Fauzan Murjianingsih, Fitriani Nurina Naqsmagita, Salsabilla Nusdianto Triakoso Pahlevy, Jolly Rifqi Pasha, Zehansyah Algifari Poedji Hastutiek Praja, Ratih Novita Prastika, Dinda Dwi Prastiya, Ragil Angga Primarizky, Hardany Pudji Srianto Puspitasari, Vina Putri, Lusi Rosita Sartono Rachmanita, Rona Taufiqul Rahmadhani, Nurmitasari Rofiqi, Mohammad Miftahur Romadhona, Ma'rifatunnisa' Safitri, Ainun Merta Santoso, Kuncoro Puguh Sarudji, Suryanie Satria, Rafif Galih Sekarsari, Dara Ayu Pramesti Setyawan, Firman Solikhah, Tridiganita Intan Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulaeman, Nur Sa'adah Sulistyowati, Deny Sunarso, Agus Tatik Hernawati Tito, Muhammad Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Trisnawati, Sharfina Habibah Tyasningsih, Wiwiek Ulinuha, Meidina Zulva Umama, Ayu Riza Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widayani, Prasita Widya Paramita Lokapirnasari Wisudawati, Feby Dwi Yudhana, Aditya Yulianto, Rangga Yunita, Maya Nurwartanti Yustinasari, Lita Rakhma