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All Journal Media Kesehatan Masyarakat Indonesia Jurnal Kebidanan Midwiferia Science and Technology Indonesia Pendas : Jurnah Ilmiah Pendidikan Dasar Jurnal Keperawatan Muhammadiyah Aptisi Transactions on Technopreneurship (ATT) Medical and Health Science Journal Jurnal Ilmu Kesehatan Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Jurnal Kebidanan Mutiara Mahakam Jurnal Penelitian Fisika dan Terapannya (JUPITER) Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Psikiatri Surabaya (Surabaya Psychiatry Journal) JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Pena : Jurnal Ilmu Pengetahuan dan Teknologi Adz Dzahab : Jurnal Ekonomi dan Bisnis Islam Indian Journal of Forensic Medicine & Toxicology Medical Journal of Al-Qodiri : Jurnal Keperawatan dan Kebidanan PROFESSIONAL HEALTH JOURNAL Buguh: Jurnal Pengabdian Kepada Masyarakat Denta: Jurnal Kedokteran Gigi Jurnal Medika: Karya Ilmiah Kesehatan Jurnal Kedokteran Gigi Terpadu Staatsrecht: Jurnal Hukum Kenegaraan dan Politik Islam Sriwijaya Journal of Dentistry Jurnal Pengabdian Kepada Masyarakat: Kesehatan (JPKMK) Pro Health Jurnal Ilmiah Kesehatan IHSAN : Jurnal Pendidikan Islam Jurnal Abdimas Indonesia Glens: Global English Insights Journal Jurnal Media Akademik (JMA) AB-JOIEC: Al-Bahjah Journal of Islamic Economics Jurnal As-Syar’i: Jurnal Bimbingan & Konseling Keluarga Gudang Jurnal Pengabdian Masyarakat Jurnal Kesehatan Masyarakat Indonesia (JKMI) Konsensus: Jurnal Ilmu Pertahanan, Hukum dan Ilmu Komunikasi
Sulistiawati
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Journal : Science and Technology Indonesia

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Development of a Gelatin-Based Genomic Reference Material for Halal Authentication Using Real-Time PCR Rahma, Anisa Aula; Meilani, Nanda Diva; Sulistiawati; Ainaputri, Aliza Salsabila; Damara, Dandy Satria; Malau, Jekmal
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.27-42

Abstract

Indonesia, home to over 270 million people, has the largest Muslim population globally, with approximately 87.18% adhering to Islam, driving significant demand for halal products, particularly in the food and pharmaceutical sectors. Gelatin, commonly used in medicinal capsules, often originates from porcine sources, necessitating precise halal authentication methods. This study presents the development of a novel genomic DNA-based Reference Material (RM) for gelatin, specifically for porcine DNA detection, employing Real-Time Polymerase Chain Reaction (qPCR) techniques. The methodology encompassed in-silico primer design, sample extraction optimization, DNA quality and quantity analysis, linearity assessment, limit of detection (LoD) and quantification (LoQ) determination, and RM characterization. Results indicated that the designed primers could reliably and efficiently detect porcine DNA, with optimal annealing at 58°C and primer concentration at 500 nM, achieving a PCR efficiency of 96.74%. The LoD and LoQ for pork meat samples were determined to be 0.02 pg/uL and 0.004 pg/uL, respectively, while the LoD for porcine gelatin was 0.27 ng/uL. The RMs exhibited robust homogeneity (Sig. 0.052), significant intergroup differences (Sig. 0.000), and low variation (CV 0.96%). Short-term storage at -80°C and -20°C preserved Ct value stability and consistency. Conclusively, this study successfully developed a novel gelatin-based genomic DNA RM for halal authentication, offering a scientifically validated tool that strengthens the halal assurance system, addressing Indonesian consumers’ demand for porcine-free products. These findings hold substantial implications for regulatory authorities, especially in Indonesia, and could inform the development of standardized qPCR RMs for porcine DNA detection in halal compliance testing.
Co-Authors Ainaputri, Aliza Salsabila Akhyar Dyni Zakyah Alfan Afandi Alfian Ananda Putra Alif Idzatulloh Alpha Fardah Athiyyah Amalia, Agnes Amelia, Rika ANAFRIN YUGISTYOWATI Anastasia, Aurora Andi M. Takdir Hasyim Angel Anggraeni, Mey Anidya Magfirah Abdullah Anis Khotimah Annin Dya Maina ARIFUDDIN Arin Nurtya Arlya Balqis Rachman Artaria Tjempakasari, Artaria Asma Asri Rismayanti Assyifa Saputri, Kirana Atikah Suci Vidyasari Aulia Sakinah Bagoes Widjanarko Bagus Styoboedi Bambang Nuryadi Bramantono Bramantono, Bramantono Budi Utomo BUDI UTOMO Bulqis Inas Sakinah Cahyanti, Ika Yuniar Chan Chow Khuen Cindy Cecilia Cindy Wahyu Agustina Damara, Dandy Satria Daniel Devi Indrian Ningsih Dewi Retno Suminar Dian Nurlita Anggraini Dicky Rahmat Kahfi Dominicus Husada Eighty Mardiyan K, Eighty Endah Ratnawati Endang Surtiyoni Endyka Erye Frety Evi Barokah Fajar Wijayati, Diska Farah Azizzah Fardal Farid, Abdul Fattah Farid, Muhammad Rifqo Hafidzudin Fathan Nursalam, Muhammad Fatimah Femmy T. Fahira Firman Adi Candra Fitri Rahmawati, Indah Fitria Ayu Cahyani Frevia Diaz Sajati Hanna Dyahferi Anomsari Hartono, Dedi Hary Nugroho I Made Subhawa Harsa Indah Dwi Sutarini Intan Nabila Intana, Rahmalia Isa Anshori Iyus YL Tobing Jayanti, Ratna Dwi Jekmal Malau Jihan hasna Quroru’aini Julius Albert Sugianto Junedi, Beni Karimah, Azimatul Lia Dian Ayuningrum Lucky Prasetiowati Lucky Wilson Immanuel Siregar Margarita Maramis, Margarita Martha Mozartha Meilani, Nanda Diva Milanti, Indah Mohammed, Datu Muh Arbai Santoso Muhajir, La Ode Muhammad Sakaum Muhammad Farhan Muhammad Farid Dimjati Lusno Muhammad Hasbi Naisyatul Khofifah Ningrum, Astika Gita Nisa, Latifatun Nita Yunita Novia Fransiska Ngo Novia Hani Safitri Nuniek Nugraheni Nur Aeni, Ani Nur Aisiyah Widjaja, Nur Aisiyah Nur Fadhilah Nur Hikmah Nurainah Nurhadijah Nurhayati, Rossa Nurlya Fitri Salsabila Nurul Fadhilah Nyilo Purnami Paloh, Suria Putri, Tania Fatimah putriani Qamara Azizah, Rossita Qurotul Aini Rafki Makarim, Ahmad Rahma, Anisa Aula Rana Gustian Nugraha Rendhi Renaldhi Ridfah, Ahmad ridwan, hardiyanti Rika Amelia Rimbun Riska Aulia Ratnadilla Risnawati Riyoko, Endie Rohmayanti Sabda Ilman, Igit Saifudin, Moh. Sairoh, Lisa Saputra Pajar, Diyas Savita, Lady Shifa Fauziyah Shifa, Relisa Nuris Sholehurrohman, Ridho Sholikhah, Siti Siti Nur Ambarini Siti Nurjanah, Lilis Suhartono Supriyanti, Dedeh Teguh Hari Sucipto, Teguh Hari Toruan, Vera Madonna Lumban Ummi Maimunah Vedisya Natasia Vinty Echi Gledisa Lase Widati Fatmaningrum Widiyawati Wiradharma, Handy Woro Ispandiyah Yufita Tarru Hegi