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All Journal Bioscience Jurnal Natur Indonesia BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Biology, Medicine, & Natural Product Chemistry Biodidaktika : Jurnal Biologi dan Pembelajarannya BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) JURNAL PENDIDIKAN ROKANIA BIOEDUSCIENCE Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Perikanan Universitas Gadjah Mada Martabe : Jurnal Pengabdian Kepada Masyarakat PeTeKa JURNAL PENDIDIKAN TAMBUSAI Mimbar Ilmu Jurnal Ilmu Kedokteran dan Kesehatan Scripta Biologica Atrium Pendidikan Biologi Pelita Eksakta Jurnal Biogenerasi Journal On Teacher Education (Jote) Jurnal Riset Biologi dan Aplikasinya 3BIO: Journal of Biological Science, Technology and Management Budapest International Research in Exact Sciences (BirEx Journal) Jurnal Biosense Journal for Lesson and Learning Studies Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Al-DYAS : Jurnal Inovasi dan Pengabdian kepada Masyarakat AHKAM : Jurnal Hukum Islam dan Humaniora Gudang Jurnal Multidisiplin Ilmu Asian Journal of Science, Technology, Engineering, and Art Tropical Genetics Jurnal Serambi Biologi Biodik: Jurnal Ilmiah Pendidikan Biologi Jurnal Natur Indonesia
Atifah, Yusni
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Padang
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Transportation Other

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DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE Hidayah, Nurul; Ahda, Yuni; Achyar, Afifatul; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5954

Abstract

EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This study aims to design EDNRB exon 4 gene primers and determine the optimum annealing temperature and primer concentration to amplify the EDNRB gene. Primer design with Geneious Prime, and good primer criteria are analyzed in silico. Optimization of annealing temperature and primer concentration is carried out with gradient PCR and variations in primer concentration. The results of this study obtained the best primers, namely the forward primer 5'- CAGTAAGTGTGGCCTGAAAG-3' and the reverse primer 5'-GTGGAACCGAAGTGACTAGA-3', which amplified the EDNRB gene exon 4. The optimum PCR conditions for amplification of exon 4 of the EDNRB gene were at an annealing temperature of 57.2 ℃ and at a primer concentration of 0.5 μM.
Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR Achyar, Afifatul; Safitri, Fira; Atifah, Yusni; Farma, Siska Alicia; Putri, Dwi Hilda; Ahda, Yuni
Halal Research Vol 5 No 2 (2025): July
Publisher : Halal Center ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j22759970.v5i2.1823

Abstract

Consumers have the right to correct information about the products they consume. One example of a food product that is vulnerable to counterfeiting is chicken nuggets. To overcome this problem, qPCR can be an effective solution. Previous research has designed primers targeting the chicken ND5 gene. However, the PCR conditions for this primer pair have never been optimized. This research aims to optimize qPCR conditions and carry out chicken nugget authentication trials. Genomic DNA was isolated from five weight variations of chicken meat samples as a qPCR standard curve and three chicken nugget samples. Optimizing the annealing temperature with gradient PCR gave the best results at a temperature of 57.6OC. The results of the authentication test showed that the chicken meat content in TD nuggets was 54.7%, C nuggets 43.8%, and D nuggets 37.3%. This value is still in accordance with the Indonesian National Standard (SNI).
Pengaruh Teratogen AlCl3 (Aluminium chloride) terhadap Perkembangan Embrio Ayam (Gallus gallus domesticus) Najib Rahman. G; Azeli, Saskia Putri; Hayu, Elvira; Ahda, Yuni; Atifah, Yusni
JURNAL BIOSENSE Vol 6 No 02 (2023): Edisi Desember 2023
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v6i02.3364

Abstract

Teratogen berefek membahayakan terhadap perkembangan embrio. Tujuan penelitian ini untuk mengetahui pengaruh teratogen AlCl3 terhadap perkembangan embrio ayam (Gallus gallus domesticus). Metode penelitian ini dilakukan secara eksperimental dengan perlakuan induksi AlCl3 0,2 mg/L dan kontrol (tanpa perlakuan). Pemberian perlakuan diberikan kepada masing-masing 5 butir telur ayam yang kemudian diinkubasi selama 48 jam. Pengamatan preparat embrio ayam menggunakan whole mount method dengan pewarnaan metilen blue dan eosin. Hasil penelitian menunjukkan ukuran embrio pada kelompok perlakuan lebih kecil (0,6 cm) dibandingkan dengan embrio kontrol (1cm). Jumlah somit pada kelompok perlakuan sejumlah 12 pasang sedangkan pada kelompok kontrol 28 pasang somit. Ukuran kepala lebih kecil jika dibandingkan dengan kelompok kontrol. Penelitian ini menunjukkan bahwa AlCl3 dapat menyebabkan malformasi pada embrio ayam, terkait dengan ukuran embrio, jumlah somit, dan ukuran kepala.
MOTILITAS DAN VIABILITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN KUMIS KUCING ( Orthosiphon aristatus) Ayesa, Putri; Fadhil Ramadhan, Bintang; Fitria, Davina; Mutia, Ravena; Ahda, Yuni; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 3 (2025): Volume 10 no 3 tahun 2025 terbit september
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i3.6781

Abstract

This study aims to evaluate the effect of cat whisker leaf extract (Orthosiphon aristatus) on motility and viability of male mice spermatozoa (Mus musculus L.). The research method used was experimental. This study was conducted to determine the effect of the treatment given to a matter under study. Consists of three treatments, namely control (without treatment), treatment dose 0.5 mg and treatment dose 0.8 mg Cat whisker leaf extract is given orally for 15 days. After treatment, spermatozoa were taken from the epididymis of male mice and tested for motility and viability using statistical analysis through the One-Way Anova test. The results showed that the decrease in motility and viability of spermatozoa depended on the dose given. The higher the dose of cat whisker extract, the motility and viability of spermatozoa decreased. In mice with a dose of 0.8 mg spermatozoan motility is silent and does not move and for viability it dies. The control treatment showed different motility but remained alive with good viability. Meanwhile, the treatment group showed a decrease in motility and viability, with control mice.
Effect of Ethanolic Extract of Avocado (Persea americana Mill.) Seed on the Testis Histology (Mus musculus l.) Induced Monosodium Glutamate (MSG) Atifah, Yusni; Zega, Dwi Junita; Ahda, Yuni; Farma, Siska Alicia
Biotik Vol 12 No 2 (2024): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v12i2.24419

Abstract

Monosodium Glutamate (MSG) is a substance that is able to produce free radicals such as ROS (Reactive Oxygen Species), which can cause oxidative stress in the body and damage reproductive organs such as the testes. The purpose of this study was to determine the effect of avocado seed ethanol extract (Persea americana Mill.) on the histology of the testes of mice (Mus musculus L.) induced monosodium glutamate (MSG). This research is an experimental study that was conducted in October 2023 – January 2024 at Biology Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang. The design used is a Complete Randomized Design (CRD) with 4 treatments and 6 repeats. The data obtained were analyzed using the ANOVA test (Analysis of variance) using the SPSS 23 application. The results showed that the administration of avocado seed ethanol extract showed a noticeable effect on the diameter of the testes of male mice exposed to MSG. However, it showed no noticeable influence on testicular weight, seminiferous tubule diameter and Leydig cell count. Ethanol extract avocado seed dose 0.84 mg /g body weight / day (P1) was able to improve the diameter of mice testes that had been induced MSGKeyword: Avocado seed extract, Histology of testis, Mice, MSG
Co-Authors Abdul Razak Abdul Razak Abubakar Abubakar Achyar, Afifatul Adinda, Diva Afrilliana, Friska Akbar, Imam Qodri Alsya Az Zahra Amalia, Windi Aprila Amsar Maulana Annisa Khaira Annisa Khaira Ardelia Febriana Ardi Ardi Arianti, Riri Putri Ayesa, Putri Az Zahra, Alsya Az zahra, Firda Azeli, Saskia Putri Azizah Mutmainah BR Tarigan, Siti Nadiah Zahra Dadang Mulyadi Saleh Des M Dezi Handayani Diana, Okta Dilla Wirmaningsih Dwi Hilda Putri Dwi Hilda Putri Dwi Junita Zega Elsa Badriyya Elsa Yuniarti Elwidani Siregar Fadhil Ramadhan, Bintang Fadika Hayyuni Fadillaturahmah, Fadillaturahmah Fathimah Azzahra Fatma Suryani Harahap Febi Permata Jingga Febiola, Cantika Riski Feby Djumaita Sari Ferix Riskierdi Fevria, Resti Fitra Arya Dwi Nugraha Fitra Nugraha, Fitra Fitri Agustina Lubis Fitri Arsih Fitria, Davina Foarota Harefa Fuadiyah, Sa’diyatul Fuji Zahara Zahara Gilang Amanda Gilang Amanda Hafizh Alza Afra Hafizh Alza Afra Hayu, Elvira Heffi Alberida Helendra Helendra . Helendra Helendra Helka Yuliati Helsa Rahmatika Hendro Pramono Iqra, Kuntum Nurul Irma Leilani Eka Putri Irma Leilani Eka Putri Iskandar Safri Hasibuan Iskandar Safri Hasibuan Jalilah Azizah Jalilah Azizah Lubis Jumatul Hafsah Keiko Kasy Billah Keiko Kasy Billah Khairunisa Khairunisa Kurnia, Aifa LAILA TUSSIFAH LUBIS Linda Advinda Lufri Lufri Marten, Threo Wanda Maulina, Adinda Rizky Mayang Anazalia Meilani Syaiful Meisya, Divia Yuda Melvariani Syari Batubara Miftahul Jannah Mita Ariani Monica, Della Trya Moralita Chatri Mutia, Ravena Mutiara Ghina Mutiara Lubis Nabila Latu Fany Najib Rahman. G Nella Fauziah Nurmaini Ginting Nurul Fathya NURUL HIDAYAH Nurul Hiza Putri Nurul Ilma Septiani Pasaribu, Surya Elita Putri Andriani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Nurul Hiza Rahma Amelia, Anisha Chahya Rahmadhani Fitri Rahmawati Darussyamsu Rahmi Holinesti Rahmi Suci Nadira Rani Mauliza Relsas Yogica, Relsas Reza Sapitri Rezi Nabilah Richi, Qori Dwi Rijal Satria Riri Putri Arianti Ristiono Ristiono Ristiono Ristiono S. Syamsurizal Sa'diatul Fuadiyah Sa'diah, Jihan Natul Sa'diah Sa’diyatul Fuadiyah safitri, fira Sahlan Tuah Saldi, Andini Putri Salsabila Juita Samsiah Samsiah Sandi Fransisco Satria, Rijal Sa’diatul Fuadiyah Silvira Ilhami Suci Fajrina Surya Elita Pasaribu Syamsurizal, S. Titisna Gumarni Vauzia Vauzia Vauzia, Vauzia Vauzia, Vauzia Verina, Farhanah Shofwah Violita Violita Violita Violita Violita Violita Widya Gusti Rahmawati D. Wulandari Wulandari Yogi Saputra, Yogi Yulia Sistina Yuliati, Netri Yuni Ahda Yuni Ahda Zahra, Fauziah Az Zega, Dwi Junita Zulfahmi Zulfahmi Zulhandri Zulhandri Zulyusri Zulyusri Zulyusri Zulyusri Zulyusri, Zulyusri Zulzusri, Zulzusri