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All Journal Bioscience Jurnal Natur Indonesia AL KAUNIYAH BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Biology, Medicine, & Natural Product Chemistry Biodidaktika : Jurnal Biologi dan Pembelajarannya BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) JURNAL PENDIDIKAN ROKANIA BIOEDUSCIENCE Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Perikanan Universitas Gadjah Mada Martabe : Jurnal Pengabdian Kepada Masyarakat PeTeKa JURNAL PENDIDIKAN TAMBUSAI Mimbar Ilmu Jurnal Ilmu Kedokteran dan Kesehatan Scripta Biologica Atrium Pendidikan Biologi Pelita Eksakta Jurnal Biogenerasi Journal On Teacher Education (Jote) Jurnal Riset Biologi dan Aplikasinya 3BIO: Journal of Biological Science, Technology and Management Budapest International Research in Exact Sciences (BirEx Journal) Jurnal Biosense Journal for Lesson and Learning Studies Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Al-DYAS : Jurnal Inovasi dan Pengabdian kepada Masyarakat AHKAM : Jurnal Hukum Islam dan Humaniora Gudang Jurnal Multidisiplin Ilmu Asian Journal of Science, Technology, Engineering, and Art Tropical Genetics Jurnal Serambi Biologi Biodik: Jurnal Ilmiah Pendidikan Biologi Jurnal Natur Indonesia
Atifah, Yusni
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Effect of Noni Extract (Morinda citrifolia L.) on Spermatozoa Quality of Mice (Mus musculus L.) Mita Ariani; Ardelia Febriana; Feby Djumaita Sari; Khairunisa Khairunisa; Yusni Atifah; Helsa Rahmatika
Jurnal Natur Indonesia Vol. 21 No. 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This study aims to see the effect of giving noni extract (Morinda citrifolia L.) on the quality of spermatozoa of mice (Mus musculus L.). This study was conducted using an experimental method, with a completely randomized design (RAL), 3 treatments with 5 replicates with 15 male mice divided into three treatment groups, namely the control group (P0), the treatment group with a concentration of 0.3 g/BW (P1), and the treatment group with a concentration of 0.6 g/BW (P2) which then the data were analyzed using ANOVA. This research was conducted from March 2 to April 4, 2023. Observations of the effect of giving noni extract were made by making wet preparations of mice sperm and then observing under a microscope. Based on the research conducted, it was found that noni extract had a different effect from each concentration on motility and morphology in sperm. From the research, it can be concluded that the higher the concentration of noni extract given to mice will reduce the quality of sperm in mice However, at a concentration of 0.3 g/BB, it was found that noni had a positive effect on sperm morphology and motility in mice (Mus musculus L).
Effect of Noni Extract (Morinda citrifolia L.) on Spermatozoa Quality of Mice (Mus musculus L.) Mita Ariani; Ardelia Febriana; Feby Djumaita Sari; Khairunisa Khairunisa; Yusni Atifah; Helsa Rahmatika
Jurnal Natur Indonesia Vol. 21 No. 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.2.87-92

Abstract

This study aims to see the effect of giving noni extract (Morinda citrifolia L.) on the quality of spermatozoa of mice (Mus musculus L.). This study was conducted using an experimental method, with a completely randomized design (RAL), 3 treatments with 5 replicates with 15 male mice divided into three treatment groups, namely the control group (P0), the treatment group with a concentration of 0.3 g/BW (P1), and the treatment group with a concentration of 0.6 g/BW (P2) which then the data were analyzed using ANOVA. This research was conducted from March 2 to April 4, 2023. Observations of the effect of giving noni extract were made by making wet preparations of mice sperm and then observing under a microscope. Based on the research conducted, it was found that noni extract had a different effect from each concentration on motility and morphology in sperm. From the research, it can be concluded that the higher the concentration of noni extract given to mice will reduce the quality of sperm in mice However, at a concentration of 0.3 g/BB, it was found that noni had a positive effect on sperm morphology and motility in mice (Mus musculus L).
Perbandingan LC50 - 72 Jam terhadap Mortalitas Ikan Cere (Gambusia Affinis) pada Limbah Sungai, Laundry, dan Oli Bengkel Adinda, Diva; Putri, Irma Leilani Eka; Atifah, Yusni
Asian Journal of Science, Technology, Engineering, and Art Vol 2 No 1 (2024): Asian Journal of Science, Technology, Engineering, and Art
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstea.v2i1.2395

Abstract

The cere fish species (Gambusia affinis) is a small fish, which is 2-3 cm. This fish is a wild fish that fills rivers, ditches and ditches. Cere fish are generally easy to adapt and reproduce. However, the water that irrigates rivers, ditches and ditches often contains community wastes, whether in the form of household waste, laundry, or even oil waste from workshops. So this study aims to obtain the LC5o-72 hour value of waste originating from river waste, laundry, and workshop oil on cere fish mortality. This research was conducted using a completely randomized design experiment. Each waste was treated in 4 treatments with different doses, namely 5 ml/L, 10 ml/L, 25 ml/L, and 50 ml/L for 72 hours with checking every 3 hours, 24 hours, 48 hours, and 72 hours. The results showed that laundry waste had a tolerance limit at a concentration of 10 ml/L and waste oil at a concentration of 25 ml/L.
Protective Effects of Apple Peel Extract (Malus sylvestris Mill.) on the Sperm Quality and Testicular Histology in Mice Borax-Induced Atifah, Yusni; Adinda, Diva; Yuliati, Netri
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p20-30

Abstract

Borax, a toxic material, can disrupt the testes, hypothalamus, and spermatogenesis in the male reproductive system, leading to infertility. Apple peel contains quercetin, a substance crucial for boosting antioxidant levels and preventing free radical damage. The study aimed to assess the impact of apple peel extract on spermatozoa quality and testicular histology in borax-induced mice. The study utilized a Complete Randomized Design (CRD) with five treatment groups and five replications of each group: C- (without treatment), C+ (Borax), T1, T2, and T3, where borax was administered alongside various doses of apple peel extract: 0,2 mg, 0,4 mg, and 0,8 mg respectively. Testicular sections were collected for histopathological analysis and caudal epididymal sections for sperm quality assessment. Data were analyzed using ANOVA and DMRT tests using SPSS 23. The results showed that apple peel extract significantly increased the quality of mice spermatozoa in borax-induced mice (p<0.05). Apple peel extract to borax-induced mice also positively affected testicular diameter and weight, as well as the diameter of the seminiferous tubules and the number of Leydig cells. The optimal dose of apple peel extract in improving the quality of spermatozoa and testicular histology of mice was found to be0.4 mg / 20g BW.
PENGARUH PEMBERIAN EKSTRAK TOMAT (Lycopersicum esculentum Mill.) TERHADAP FERTILITAS MENCIT BETINA (Mus musculus L.) Rani Mauliza; Az Zahra, Alsya; Verina, Farhanah Shofwah; Andriani, Putri; Yusni Atifah; Rahmatika, Helsa
JURNAL BIOSENSE Vol 8 No 1 (2025): Edisi Januari 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i1.4884

Abstract

Abstrak Fertilitas merupakan hal yang sangat penting pada wanita untuk menghasilkan keturunan. Salah satu dari banyaknya faktor kesuburan wanita adalah nutrisi yang dikonsumsinya. Buah tomat (Lycopersicum esculentum Mill.) mengandung senyawa likopen yang memiliki banyak manfaat diantaranya sebagai zat antioksidan, menjaga kestabilan sel serta menurunkan resiko infertilitas. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak tomat (Lycopersicum esculentum Mill.) terhadap fertilitas mencit betina (Mus musculus L.). Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari tiga perlakuan dengan lima ulangan. Perlakuan kontrol (P0) yang tidak diberi esktrak tomat, perlakuan 1 (P1) dan perlakuan 2 (P2) dengan dosis ekstrak tomat 0,4 ml/hari dan 0,8 ml/hari. Pemberian ekstrak tomat, dilakukan setiap hari selama 15 hari secara oral menggunakan spuid modifikasi. Hasil penelitian menunjukkan adanya pengaruh ekstrak tomat (Lycopersicum esculentum Mill.) pada fertilitas mencit betina. Pada pemberian dosis 0,4 ml/hari menunjukkan terjadinya peningkatan kesuburan mencit pada fase estrus dibandingkan dengan kelompok perlakuan lainnya. Pemberian ekstrak tomat (Lycopersicum esculentum Mill.) dapat meningkatkan kualitas kesuburan mencit betina. Kata kunci: Fertilitas, Lycopersicum esculentum Mill., Mencit, Betina, Estrus
PENGARUH PENGENCERAN AIR KELAPA MUDA DAN MADU KONSENTRASI BERBEDA TERHADAP MOTILITAS DAN MORFOLOGI SPERMATOZOA IKAN NILA (Oreochromis niloticus) Alsya Az Zahra; Febi Permata Jingga; Nurul Fathya; Yusni Atifah
JURNAL BIOSENSE Vol 8 No 1 (2025): Edisi Januari 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i1.4994

Abstract

Preservasi spermatozoa merupakan langkah penting dalam teknologi reproduksi ikan untuk mendukung program budi daya dan pelestarian spesies. Air kelapa muda memiliki komposisi elektrolit dan mineral yang mirip dengan cairan fisiologis, sehingga menjadi solusi pengencer alami yang potensial untuk pengawetan spermatozoa. Glukosa dalam madu berfungsi sebagai sumber energi untuk mempertahankan motilitas. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pengenceran dengan air kelapa muda dan konsentrasi madu yang berbeda terhadap motilitas dan morfologi spermatozoa ikan nila. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dan 5 kali ulangan. Jenis pengencer yang digunakan terdiri dari air kelapa muda dan madu dengan berbagai konsentrasi, yaitu 0%, 3%, 6%, dan 9%. Data yang diperoleh mencakup motilitas (%) dan morfologi kemudian dianalisis secara statistik dengan One-way ANOVA dan morfologi spermatozoa ikan nila. Nilai kepercayaan data adalah 95% dengan nilai P-value <0,05. Hasil penelitian menunjukkan bahwa perlakuan pengenceran air kelapa muda dan madu dengan dosis 3% berpengaruh terhadap motilitas spermatozoa ikan nila selama 7 hari penyimpanan. Penelitian ini dapat digunakan sebagai referensi untuk pengawetan spermatozoa.
The Improvement of Heart Histopathology of Hypercholesterolemic Mice using Sidempuan Salacca (Salacca sumatrana) Vinegar Atifah, Yusni; Az zahra, Firda; Helendra, Helendra; Lubis, Jalilah Azizah; Afrilliana, Friska
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.161-166

Abstract

Hypercholesterolemic, a condition characterized by excess cholesterol content, can lead to coronary heart disease, atherosclerosis, and high-risk heart tissue degeneration. Current drugs, like simvastatin and pravastatin, focus on lowering cholesterol levels without addressing tissue degeneration. This study aimed to determine the effectiveness of sidempuan salacca vinegar in improving cardiac tissue degeneration in hypercholesterolemic mice. The experimental research involved two groups, the treatment group given different doses of sidempuan salacca vinegar and the control group. Histopathological observations showed improvement in cardiac tissue degeneration in hypercholesterolemic mice after treatment with salacca vinegar. The administration of salacca vinegar could serve as a reference for developing alternative hypercholesterolemic drugs that address both cholesterol levels and heart degeneration.
DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE Hidayah, Nurul; Ahda, Yuni; Achyar, Afifatul; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5954

Abstract

EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This study aims to design EDNRB exon 4 gene primers and determine the optimum annealing temperature and primer concentration to amplify the EDNRB gene. Primer design with Geneious Prime, and good primer criteria are analyzed in silico. Optimization of annealing temperature and primer concentration is carried out with gradient PCR and variations in primer concentration. The results of this study obtained the best primers, namely the forward primer 5'- CAGTAAGTGTGGCCTGAAAG-3' and the reverse primer 5'-GTGGAACCGAAGTGACTAGA-3', which amplified the EDNRB gene exon 4. The optimum PCR conditions for amplification of exon 4 of the EDNRB gene were at an annealing temperature of 57.2 ℃ and at a primer concentration of 0.5 μM.
Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR Achyar, Afifatul; Safitri, Fira; Atifah, Yusni; Farma, Siska Alicia; Putri, Dwi Hilda; Ahda, Yuni
Halal Research Vol 5 No 2 (2025): July
Publisher : Halal Center ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j22759970.v5i2.1823

Abstract

Consumers have the right to correct information about the products they consume. One example of a food product that is vulnerable to counterfeiting is chicken nuggets. To overcome this problem, qPCR can be an effective solution. Previous research has designed primers targeting the chicken ND5 gene. However, the PCR conditions for this primer pair have never been optimized. This research aims to optimize qPCR conditions and carry out chicken nugget authentication trials. Genomic DNA was isolated from five weight variations of chicken meat samples as a qPCR standard curve and three chicken nugget samples. Optimizing the annealing temperature with gradient PCR gave the best results at a temperature of 57.6OC. The results of the authentication test showed that the chicken meat content in TD nuggets was 54.7%, C nuggets 43.8%, and D nuggets 37.3%. This value is still in accordance with the Indonesian National Standard (SNI).
Pengaruh Teratogen AlCl3 (Aluminium chloride) terhadap Perkembangan Embrio Ayam (Gallus gallus domesticus) Najib Rahman. G; Azeli, Saskia Putri; Hayu, Elvira; Ahda, Yuni; Atifah, Yusni
JURNAL BIOSENSE Vol 6 No 02 (2023): Edisi Desember 2023
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v6i02.3364

Abstract

Teratogen berefek membahayakan terhadap perkembangan embrio. Tujuan penelitian ini untuk mengetahui pengaruh teratogen AlCl3 terhadap perkembangan embrio ayam (Gallus gallus domesticus). Metode penelitian ini dilakukan secara eksperimental dengan perlakuan induksi AlCl3 0,2 mg/L dan kontrol (tanpa perlakuan). Pemberian perlakuan diberikan kepada masing-masing 5 butir telur ayam yang kemudian diinkubasi selama 48 jam. Pengamatan preparat embrio ayam menggunakan whole mount method dengan pewarnaan metilen blue dan eosin. Hasil penelitian menunjukkan ukuran embrio pada kelompok perlakuan lebih kecil (0,6 cm) dibandingkan dengan embrio kontrol (1cm). Jumlah somit pada kelompok perlakuan sejumlah 12 pasang sedangkan pada kelompok kontrol 28 pasang somit. Ukuran kepala lebih kecil jika dibandingkan dengan kelompok kontrol. Penelitian ini menunjukkan bahwa AlCl3 dapat menyebabkan malformasi pada embrio ayam, terkait dengan ukuran embrio, jumlah somit, dan ukuran kepala.
Co-Authors Abdul Razak Abdul Razak Abubakar Abubakar Achyar, Afifatul Adinda, Diva Afrilliana, Friska Akbar, Imam Qodri Alsya Az Zahra Amalia, Windi Aprila Amsar Maulana Annisa Khaira Annisa Khaira Ardelia Febriana Ardi Ardi Arianti, Riri Putri Ayesa, Putri Az Zahra, Alsya Az zahra, Firda Azeli, Saskia Putri Azizah Mutmainah Azizah, Jalilah BR Tarigan, Siti Nadiah Zahra Dadang Mulyadi Saleh Des M Dezi Handayani Diana, Okta Dilla Wirmaningsih Dwi Hilda Putri Dwi Hilda Putri Dwi Junita Zega Elsa Badriyya Elsa Yuniarti Elwidani Siregar Fadhil Ramadhan, Bintang Fadika Hayyuni Fadillaturahmah, Fadillaturahmah Fathimah Azzahra Fatma Suryani Harahap Febi Permata Jingga Febiola, Cantika Riski Feby Djumaita Sari Ferix Riskierdi Fevria, Resti Fitra Arya Dwi Nugraha Fitra Nugraha, Fitra Fitri Agustina Lubis Fitri Arsih Fitria, Davina Foarota Harefa Fransisco, Sandi Fuadiyah, Sa’diyatul Fuji Zahara Zahara Gilang Amanda Gilang Amanda Hafizh Alza Afra Hafizh Alza Afra Hayu, Elvira Heffi Alberida Helendra Helendra . Helendra Helendra Helka Yuliati Helsa Rahmatika Hendro Pramono Iqra, Kuntum Nurul Irma Leilani Eka Putri Irma Leilani Eka Putri Iskandar Safri Hasibuan Iskandar Safri Hasibuan Jalilah Azizah Jalilah Azizah Lubis Juita, Salsabila Jumatul Hafsah Keiko Kasy Billah Keiko Kasy Billah Khairunisa Khairunisa Khairunisa Khairunisa Kurnia, Aifa LAILA TUSSIFAH LUBIS Linda Advinda Lufri Lufri Marten, Threo Wanda Maulina, Adinda Rizky Mayang Anazalia Meilani Syaiful Meisya, Divia Yuda Melvariani Syari Batubara Miftahul Jannah Mita Ariani Mita Ariani Monica, Della Trya Moralita Chatri Mutia, Ravena Mutiara Ghina Mutiara Ghina Mutiara Lubis Nabila Latu Fany Nabilah, Rezi Najib Rahman. G Nella Fauziah Nurmaini Ginting Nurul Fathya NURUL HIDAYAH Nurul Hiza Putri Nurul Ilma Septiani Pasaribu, Surya Elita Pratama, Sandi Fransisco Putri Andriani Putri, Aulia Devani Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Nurul Hiza Rahma Amelia, Anisha Chahya Rahmadhani Fitri Rahmawati Darussyamsu Rahmi Holinesti Rahmi Suci Nadira Rani Mauliza Relsas Yogica, Relsas Reza Sapitri Rezi Nabilah Richi, Qori Dwi Rijal Satria Riri Putri Arianti Ristiono Ristiono Ristiono Ristiono S. Syamsurizal Sa&#039;diatul Fuadiyah Sa'diah, Jihan Natul Sa'diah Sa’diyatul Fuadiyah safitri, fira Sahlan Tuah Saldi, Andini Putri Samsiah Samsiah Satria, Rijal Sa’diatul Fuadiyah Silvira Ilhami Suci Fajrina Surya Elita Pasaribu Syamsurizal, S. Titisna Gumarni Vauzia Vauzia Vauzia, Vauzia Vauzia, Vauzia Verina, Farhanah Shofwah Violita Violita Violita Violita Violita Violita Widya Gusti Rahmawati D. Wulandari Wulandari Yogi Saputra, Yogi Yulia Sistina Yuliati, Netri Yuni Ahda Yuni Ahda Zahra, Fauziah Az Zega, Dwi Junita Zulfahmi Zulfahmi Zulhandri Zulhandri Zulyusri Zulyusri Zulyusri Zulyusri Zulyusri, Zulyusri Zulzusri, Zulzusri