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EFFECT OF SALINITY CHANGES ON METHIONINE CONTENT IN TIGER GROUPER JUVENILE (Epinephelus fuscoguttatus) Abbas, Muhammadar Abdullah; Abidin, Muchlisin Zainal; Putra, Dedi Fazriansyah; Firdus, Firdus; Batubara, Agung Setia; Sail, Asmawati Muhammad; Syafruddin, Syafruddin; Roslizawaty, Roslizawaty; Sutriana, Amalia; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.12363

Abstract

This study was conducted to determine the effect of water salinity changes on methionine content in tiger grouper juvenile (Epinephelus fuscoguttatus). A total of 2,560 tiger grouper juveniles were used and divided into five groups consist of 1 control group (without exposed to salinity changes) and 4 treatment groups. The salinity was changed every 2, 3, 4, and 6 hours in treatment A, B, C, and D, respectively. Salinity levels were changed during 24 hours by lowering salinity level from 32 psu to 22 psu. Twenty five of fish were collected from each treatment for methionine content analysis. Data were analysed using one way analysis of variance (ANOVA). The results showed that there was a decrease in methionine content in all treatments. A significant decrease (P0.05) of methionine content in treatment A, B, and C were observed after 20 hours (1.15%), 18 hours (1.27%), and 16 hours (1.24%), respectively. While at 0 hours (control), the methionine content was 2.02%. Methionine level in treatment D was not significantly different (P0.05) compared to control group. As conclusion, rearing the tiger grouper juvenile with salinity fluctuations every 6 hours did not lead to methionine deficiency.
Molecular characterization of Tapah fish (Wallago leerii, Bleeker 1851) from Riau Province based on Cytochrome b gen Elvyra, Roza; Nurasiah, Nurasiah; Batubara, Agung Setia
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.33161

Abstract

Tapah fish (Wallago leerii) is a fish with high economic value in Riau Province. Molecular research on W. leerii is very important to do. This study aims to determine the molecular characteristics of W. leerii from Riau Province based on the Cytochrome b gene. The universal primer Cytochrome b is used for the Polymerase Chain Reaction (PCR) process. The PCR result is a partial Cytochrome b gene with a length of 373 bp. Multiple alignments were performed on nucleotide sequences of the Cytochrome b gene of W. leerii from river of Tapung and Indragiri Riau with the cytochrome b gene of other fish from Genbank and analyzed using the MEGA program version 6.0. Phylogenetic construction using the Cytochrome b gene can distinguish W. leerii from river of Tapung and Indragiri Riau, with other species.Keywords:AlignmentsMolecularGenBank
Cytotoxicity of leaves, stems, and flowers of Kecubung (Datura metel) extracts using the Brine Shrimp Lethality Test (BSLT) method Humairani, Rindhira; Purnama, Nanda Rizki; Herpandi, Herpandi; Syaifudin, Mochamad; Zulfahmi, Ilham; Akmal, Yusrizal; Muliari, Muliari; AS, Agus Putra; Batubara, Agung Setia
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.27445

Abstract

Brine shrimp (Artemia salina) cytotoxicity assays (BSLT) are one of the commonly used cytotoxic test methods to assess a plant extract's pharmacological activity and toxicity. Thus, this study aimed to examine the toxic levels of Kecubung (Datura metel) leaves extracted using different solvents, namely ethanol and ethyl acetate. The results showed that the LC50value of flower ethanol, flower ethyl acetate, leaf ethanol, and stem ethyl acetate extracts had an LC50value 1000, which was included in the toxic category. The LC50value of flower ethanol is 121.044 ppm, flower ethyl acetate 105.89 ppm, leaf ethanol 639.589 ppm, and stem ethyl acetate 635.276 ppm. Ethanol leaf extract at a concentration of 1000 ppm showed the highest mortality with a percentage of 60% of the total number ofA. salina. The flower ethanol extract showed the highest mortality at a concentration of 250 ppm with a percentage of 67% and at a concentration of 1000 ppm with a percentage of 70% of the total number ofA. salina. Meanwhile, flower ethyl acetate and stem ethyl acetate extract at a concentration of 1000 ppm obtained 100% mortality ofA. salinain the first 6 hours. The result shows that the flower ethyl acetate and stem ethyl acetate extract at a concentration of 1000 ppm is very toxic compared to other concentrations.Keywords:ToxicityArtemia salinaLC50Ethyl acetateEthanol
Diversity and distribution of fish in the Lokop river, Leuser Ecosystem Area, Indonesia Maghfiriadi, Furqan; Firdus, Firdus; Sarong, M. Ali; Zulfahmi, Ilham; Batubara, Agung Setia
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 1 (2023): APRIL 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.1.30181

Abstract

Lokop is one of the villages in Serbajadi District, East Aceh Regency, Aceh Province. The village has a watershed named the river Lokop. This river is located in the Leuser Ecosystem Area which is inhabited by various aquatic biota, especially fish, with the level of diversity and distribution patterns that need to be studied and analyzed conoftened. This study used the purposive sampling method, with the parameters being the level of diversity and distribution patterns of fish in the Lokop river. Data collection was carried out at 5 observation stations based on the typology of river habitat, each station was divided into three substations representing parts of the basin, river bank and river basin approximately 50 m apart. Fish sampling is carried out at each station using a plot of 10 x 10 m2. The number of plots at each station is 9 plots with a total of 45 plots. The results showed that the diversity index (H') value of fish in the Lokop river was 2.39 in the moderate category and the distribution pattern of fish in the Lokop river has a uniform pattern and clusters. Out of a total of 17 species obtained, 8 species (47%) were clustred, 8 species (47%) were uniformly distributed and 1 species (6%) could not be analyzed because only one individual was found.Keywords:FishRiverDiversityDistribution
Analisis Kelimpahan Mikroplastik Pada Lima Spesies Ikan Laut Dari TPI Pantai Labu, Kecamatan Pantai Labu Kabupaten Deli Serdang Butarbutar, Yutrina; Batubara, Agung Setia; Sudibyo, Mufti
BEST Journal (Biology Education, Sains and Technology) Vol 9, No 1 (2026): Juni 2026
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v9i1.12904

Abstract

Penelitian ini bertujuan untuk mengidentifikasi bentuk, warna, dan kelimpahan mikroplastik pada lima spesies ikan (Rastrelliger kanagurta, Nemipterus japonicus, Sardinella fimbriata, Megalaspis cordyla, Eleutheronema tetradactylum). Penelitian ini dilakukan dari Juni hingga Agustus 2025, dimana sampel diperoleh dari TPI Pantai Labu. Analisis mikroplastik dilakukan di laboratorium Biologi, FMIPA Universitas Negeri Medan. Proses ekstraksi mikroplastik dimulai dengan memasukkan organ ikan target kedalam larutan H2O2 30% sebanyak 20 ml dan FeSO4  0,05M sebanyak 5 tetes kemudian diinkubasi selama 24 jam pada suhu 40ºC. Selanjutnya organ ikan yang telah terurai disaring menggunakan Whatman, dibilas menggunakan larutan NaCl jenuh, dan hasil saringan ditampung ke dalam cawan petri. Sampel yang telah dikeringkan selanjutnya diamati menggunakan mikroskop stereo. Hasil analisis mikroplastik pada lima spesies ikan menunjukkan kontaminasi, dengan nilai kelimpahan total tertinggi pada spesies Rastrelliger kanagurta (58,7 partikel/individu), diikuti Megalaspis cordyla (44,3), Sardinella fimbriata (31,5), Eleutheronema tetradactylum (29,6), dan Nemipterus japonicus (29,3 partikel/individu). Hasil analisis mikroplastik menunjukkan bentuk mikroplastik yang ditemukan terdiri atas tiga bentuk, yaitu fiber, fragmen, dan film dengan dominasi bentuk fiber dan warna hitam pada seluruh spesies ikan. Selanjutnya hasil uji one way ANOVA menunjukkan bahwa jenis ikan berpengaruh nyata terhadap kelimpahan mikroplastik (p 0,05), di mana R. kanagurta berbeda signifikan dengan N. japonicus, S. fimbriata, dan E. tetradactylum, sedangkan ketiga spesies terakhir tidak berbeda nyata satu sama lain
Co-Authors Abbas, Muhammadar Abdullah Abdullah A. Muhammadar Abdullah Abdullah Abidin, Muchlisin Zainal Agus Putra AS Akhmad Mulyadi Ali Sarong Amalia Sutriana Asmawati Asmawati ButarButar, Yutrina Dawami, Muhammad Dedi Fazriansyah Putra Dwinna Aliza Dwinna Aliza Elida Hafni Siregar, Elida Hafni Fauziah Fauziah Firdus Firdus Firdus Firdus Firdus Firdus Gunawan, Safri Hani Plumeriastuti Herpandi . Humairani, Rindhira Iin Siti Djunaidah Ikram, Said Ilham Zulfahmi Irfannur Irfannur Irfannur Irfannur, Irfannur Irma Dewiyanti Izwar, Akmal Januariyansah, Sapitri julismi, Al Lili Kasmini Lubis, Khairiza Maghfiriadi, Furqan Manullang, Lydia Ruth Hasianna Maulizar, Siti Mochamad Syaifudin, Mochamad Muchlisin Zainal Abidin Mufti Sudibyo, Mufti Muhammad Y. Thamren Muhammad Yunus Thamren Mujibul Rahman Muliari Muliari Muliari Muliari Muliari Nafis, Badratun Nasution, Debi Rausana Nur Fadli Nur, Firman M Nurasiah Nurasiah Nurfadillah Nurfadillah Perdana, Adli Waliul Plumeriastuti , Hani Purnama, Nanda Rizki Putra, Dedi Fazriansyah Rahmatsyah Rajo Hasim Lubis Rita Juliani Roslizawaty Roslizawaty Roslizawaty Roslizawaty, Roslizawaty Roza Elvyra Roza, Zultira Harina Sail, Asmawati Muhammad Sani, Ridwan A Saragih, Ferosalina Natasia Silitonga, Afrizal Bigman Sinung Rahardjo Siska Mellisa Solly Aryza Supriatno Supriatno Syafruddin Syafruddin Syafruddin Syafruddin Usnardi Usnardi Waliul, Adli Yeni Dhamayanti Yunus , Muchammad Yunus, Muchammad Yusrizal Akmal Zainal A. Muchlisin Zulkifli, Arief Hidayat