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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal MIPA JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) PROSIDING SEMINAR KIMIA Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Bioma : Jurnal Biologi Indonesia ARSHI Veterinary Letters Jambura Physics Journal JCPS (Journal of Current Pharmaceutical Sciences) Jurnal Fisika dan Terapannya Jurnal Abdimas ADPI Sains dan Teknologi Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Jurnal Veteriner Nusantara Current Biomedicine

koekoeh santoso

IPB University

Author-ID : 2359674

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Energy Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Respons Imun Puyuh (Coturnix coturnix Japonica) Dewasa yang Mendapat Ekstrak Daun Singkong dalam Mengatasi Dampak Cekaman Panas (IMMUNE RESPONSE OF ADULT QUAILS (COTURNIX COTURNIX JAPONICA) TREATED WITH CASSAVA LEAF EXTRACT TO OVERCOME HEAT STRESS) Koekoeh Santoso; Anindita Sista Widyadhari; Okti Nadia Poetri; La Jumadin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of the research was to prove the potency of cassava leaf extract to overcome heat stress of adult quails on the variable of antibody titer of Newcastle Disease (ND), MDA level, and total protein. The research was divided into four groups and conducted in 6 replications for each group, consisting of control group, group A, B, and C. All the groups were exposed to heat stress, and then treated with cassava leaf extract with different dosages for 5,292 mg/168 g body weight, 10,584 mg/168 g body weight, and 21,168 mg/168 g body weight for 28 days after being adapted for a week. Variables of antibody titer of Newcastle Disease (ND), MDA level, and total protein were measured every week. The result showed that HI titer of overall tested groups was less than 2 HI units. HI titer contains positive antibody of antibody titer of ND if it reaches 16 HI units. HI test in the present study showed that all of quail serums contained negative antibody of ND. The level of MDA fluctuated with the highest and smallest value was found in group B and control group, respectively. In addition, the administration of cassava leaves extract tended to decrease total protein, where control group was significantly different to both group A and C (P < 0,05). In conclusion, cassava leaves extract has the potential to decrease the stress level, but the administration up to 21,168 mg/168 g BB has not been able to decrease the level of MDA in quails that suffered from heat stress. In Dosage 21, 168 mg extract of cassava leaves was potential to lower stress level so that it was unable to stimulate immune respond to form ND titer antibody.

Ekstrak Daun Singkong Berpotensi Sebagai Antioksidan pada Burung Puyuh yang Mendapat Cekaman Panas Singkat La Jumadin; Aryani Sismin Satyaningtijas; Zulvia Maika; Lili Darlian; Warysatul Ummah; Koekoeh Santoso
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.235 KB)

The purpose of this study was to explore the potential of cassava leaves extract as an antioxidant in quail (Coturnix coturnix japonica) females as a result of heat exposure. The parameter are total erythrocyte count, hematocrit value, hemoglobin concentration and erythrocyte index (MCV, MCH, MCHC). Quails were devided into six groups. The control group (K0) was not given cassava leaves extract and heat. Treatment group (KL) was only given chlorophyll cassava leaves extract dose 5,29 mg/g. Group P was only given heat exposure. Group P+KL1, P+KL2, and P+KL3 were exposed to heat and given the doses of cassava leaves extract 5,29 mg, 10,58 mg, and 21,16 mg/168 g body weight, respectively orally for 28 days after a week of adaptation. Parameters such as the total erythrocyte count, hematocrit value, hemoglobin concentration and erythrocyte index (MCV, MCH, MCHC) were observed every two weeks. Results showed that the mean of eritrosit ranged from 8,64 x 106/mm3 in K0 to 10,47 x 106/mm3 in P+KL3. Hematocrit value in all treatment groups did not increase. Temperature exposure and paste of cassava leaves with different level of dosages were not significantly different from MCV (P>0,05). The highest level of MCV was obtained in treatment K0 (0,48 fl). The highest level of MCH ranged from 5,83-6,33 pg. The highest level of MCH was obtained in treatment P (6,33 pg). The highest level of MCHC was obtained in treatment P (63,38 %). The mean of MCHC level in quail treated with the paste of cassava leaves tended to decrease compared to those were not treated with the paste. Conclusion of this study is cassava leaf extract has potential as an antioxidant in adult quail given short heat exposure. The application of chlorophyll extracted from cassava leaves did not significantly affect the quantity of blood cells, haemoglobin level, hematocrit value and eritrosite index in adult quail treated by short thermal stress.

Profil Leukosit Puyuh (Coturnix coturnix japonica) yang Mendapat Ekstrak Daun Singkong dalam Mengatasi Dampak Cekaman Panas Koekoeh Santoso; Anindita Sista Widyadhari; Okti Nadia Poetri; La Jumadin
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.141 KB)

Penelitian ini bertujuan mengkaji potensi daun singkong (Manihot esculenta) dalam mengatasi cekaman panas pada puyuh terhadap parameter total leukosit, diferensiasi leukosit dan indeks stres. Penelitian ini dibagi menjadi empat kelompok. Kelompok kontrol serta Kelompok A, B, dan C masing-masing mendapat cekaman panas, kemudian diberi ekstrak klorofil daun singkong 5,292, 10,584, dan 21,168 mg/ 168 g bobot badan per oral selama 28 hari setelah diadaptasikan satu minggu. Parameter seperti total leukosit, diferensiasi leukosit dan indeks stres dilakukan setiap minggu. Hasil penelitian menunjukkan bahwa menunjukkan jumlah leukosit mengalami penurunan yang tidak berbeda nyata sejalan dengan peningkatan dosis ekstrak. Limfosit kelompok perlakuan ekstrak daun singkong (A, B, dan C) lebih tinggi (P < 0,05) dibandingkan kontrol, sedangkan heterofil kelompok perlakuan ekstrak daun singkong (A, B, dan C) lebih rendah (P < 0,05) dibandingkan kontrol. Monosit dan eosinofil kelompok perlakuan ekstrak daun singkong (A, B, dan C) cenderung lebih rendah dibandingkan kontrol. Pengamatan basofil menunjukkan hasil yang tidak berbeda nyata (P < 0,05) antara kontrol dengan kelompok perlakuan ekstrak daun singkong. Hasil penelitian lain menunjukkan bahwa semakin tinggi nilai H/L maka semakin tinggi tingkat stres hewan. Rasio H/L tertinggi terlihat pada kelompok kontrol, yang diikuti dengan kelompok perlakuan A, B, dan C. Penurunan tingkat stres puyuh teramati sejalan dengan peningkatan dosis ekstrak daun singkong yang diberikan. Simpulan pada penelitian ini adalah pemberian ekstrak daun singkong mampu menurunkan total leukosit serta rasio H/L. Selain itu, pemberian ekstrak daun singkong cenderung menurunkan monosit, heterofil, dan eosinofil serta meningkatkan limfosit.

The Effectiveness of Red Yeast Rice Antioxidant on Performance of Japanese Quail (Coturnix coturnix japonica) Induced Oxidative Stress through Dexamethasone Administration Nuri Ardiani; Koekoeh Santoso; Hera Maheshwari
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Red yeast rice is a fermented product of rice and mold Monascus purpureus. Red yeast rice contains secondary metabolites such as lovastatin, phenol and flavonoids that have potential as antioxidants. This study was aimed to examine the effectiveness of red yeast rice antioxidants on performance of japanese quail administered with dexamethasone through the parameters of feed digestibility, feed consumption, growth in body weight, stress index, malondialdehyde (MDA) andsuperoxide dismutase (SOD). This study used a factorial completely randomized design. The animals used in this study were 60 quails (Coturnix coturnix japonica), with a body weight of 140195 grams. Group K0 was a control, group D were quails given only dexamethasone at a dose of 1.25 mg/g BW for 7 days after one-week adaptation. Group D+VC were quails given dexamethasone at a dose of 1.25 mg/g BW then added with vitamin C at a dose of 20 mg. Group D+K1, D+K2, and D+K3 were quails given dexamethasone then orally given red yeast rice of 18 mg, 24 mg, and 30 mg for 14 days. The feed consumption, feed digestibility and body weight shows no significant difference among group (P>0.05). The heterophyl/lympocyte ratio was elevated when dexametasone administered and tended to decrease when administered with red yeast rice. Result also showed that red yeast rice has the potential as an antioxidant in quail administered with dexamethasone as seen from the tendency of decreased level of MDA and increased level of SOD enzyme. The results also showed that quail administered with dexamethasone decreased level of MDA and elevated levels of SOD enzyme in the liver after administration of red yeast rice.

Perbandingan Deteksi Titer Antibodi Pascavaksinasi Rabies Berbasis Kolorimetri Menggunakan ELISA Reader dan Kamera Telepon Genggam Koekoeh Santoso; Ulfatin Khoiriyah Herowati; Dordia Anindita Rotinsulu; Sri Murtini; Muhammad Yusuf Ridwan; Denny Widya Hikman; Abdul Zahid; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Agus Wijaya; Ridi Arif; Ronald Tarigan; Edi Sukmawinata
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Rabies is an infectious disease, zoonotic, caused by virus from the genus Lyssa virus and generally transmitted by the bite of rabid animal, especially rabies infected dog. Rabies is preventable but is always fatal to humans if the central nervous system (CNS) is infected. Vaccination has been used as one of rabies prevention programmed. A total of 83 samples were tested using an Indirect ELISA (Enzyme Linked Immunosorbent Assay) to identify post-vaccination rabies antibody titer. Antibody titres correlated with absorbance values and standard solutions concentrations. Absorbance value can be determined using ELISA reader and mobile phone camera. Absorbance were read at 450 nm and 620 nm as reference using ELISA reader and image from mobile phone camera using image processing software (ImageJ). The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy. There is no significant difference between gold standard and alternative test equipment. The mobile phone camera has sensitivity 98,6%, specificity 88.8 % and accuracy 97,5%. The image processing method using ELISA reader is relatively expensive and difficult to hold in laboratory with minimum funds. Image processing method using a mobile phone camera with ImageJ application is expected to be an alternative tool to read the result of ELISA.

Ekstrak Daun Singkong Baik Sebagai Antioksidan pada Burung Puyuh Dewasa yang Mendapat Paparan Panas Singkat (EXTRACT OF CASSAVA LEAVES IS A GOOD ANTIOXIDANT FOR MATURE QUAIL WHICH EXPOSED TO HEAT IN SHORT TIME) La Jumadin; Aryani Sismin Satyaningtijas; Koekoeh Santoso
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aims of this study was to investigate the potential use of chlorophyll extracted form cassava leaves as antioxidant for quail (Coturnix coturnix japonica). The experimental design adopted in this study was simple randomized design consisting of six treatments. The six treatments consist of chlorophyll extract at the dose 5,29 mg/168 g body weight (KL), animal exposed to 40oC for 8 hours daily (P), animal exposed to 40oC for 8 hours daily treated with chlorophyll extract at the dose of 5,29 (P+KL1), at the dose of 10,58 (P+KL2, and at the dose of 21,16 mg/168 g (P+KL3). The treatment was conducted for 28 days following 7 days adaptation period. Parameters observed in this study was feed consumption, feed digestibility, body weight, the number of eggs, egg weight, the level of yolk, the level of albumin, the weight of ovaries and uterus, and the thickness of egg shell. The results showed that quail exposed to 40oC for 8 hours daily (P) had a lower level of feed consumption as compared to those of animal unexposed to 40oC heat (P0). The highest level of feed digestibility was observed in KL treatment group. The average body weight tended to increase in quail treated with chlorophyll (KL) as compared to those of K0 and P treatment groups. The number of eggs and egg weight increased in quail exposed to 40oC and treated with chlorophyll extract. The level of yolk and albumin tend to increase in quail treated chlorophyll (P+KL) as compared to those of K0 and P. The average weights of ovary and uterus were not significantly different among all treatment groups (P>0,05). The thinnest egg shell was observed in P treatment group. The result of this study shows that the chlorophyll has antioxidant potential for adult quail exposed briefly to heat. ABSTRAK Penelitian ini bertujuan mengkaji potensi ekstrak klorofil daun singkong sebagai antioksidan pada burung puyuh (Coturnix coturnix japonica) dewasa yang dipapar panas singkat. Adapun peubah yang diamati adalah konsumsi pakan, kecernaan pakan, bobot badan, jumlah telur, bobot telur, tinggi kuning telur, tinggi putih telur/albumen, bobot ovarium, bobot uterus, dan tebal kerabang telur. Penelitian ini terdiri dari enam kelompok perlakuan. Kelompok kontrol (K0), kelompok hewan uji hanya diberi ekstrak klorofil daun singkong 5,29 mg/168 g bobot badan/oral (KL). Kelompok hewan uji dipapar suhu 40oC selama delapan jam tiap hari (P). Kelompok P+KL1, P+KL2, dan P+KL3 masing-masing dipapar suhu 40oC selama delapan jam tiap hari, kemudian diberi ekstrak klorofil daun singkong 5,29, 10,58, dan 21,16 mg/168 g bobot badan per oral selama 28 hari setelah diadaptasikan satu minggu. Parameter seperti konsumsi pakan, kecernaan pakan, jumlah telur, dan bobot telur dihitung setiap hari selama penelitian. Parameter lain diukur pada akhir perlakuan, kecuali bobot badan dilakukan setiap minggu. Hasil penelitian menunjukkan bahwa burung puyuh yang mendapatkan paparan panas (P) konsumsi pakannya cenderung menurun dibandingkan kelompok K0. Rataan kecernaan pakan tertinggi dijumpai pada kelompok KL. Rataan bobot badan burung puyuh yang mendapatkan ekstrak klorofil daun singkong cenderung meningkat dibandingkan dengan perlakuan K0 dan P. Rataan jumlah dan bobot telur pada kelompok yang mendapatkan paparan panas dan ekstrak klorofil daun singkong cenderung meningkat dibandingkan kelompok K0, kecuali pada kelompok P+KL3. Rataan tinggi kuning telur/yolk dan albumen dari kelompok burung puyuh yang mendapat ekstrak klorofil daun singkong cenderung lebih tinggi dibandingkan kelompok K0 dan P. Rataan bobot ovarium dan uterus pada semua kelompok perlakuan tidak menunjukkan perbedaan (P>0,05). Rataan tebal kerabang telur pada kelompok P paling tipis dibandingkan yang lain. Simpulan pada penelitian ini adalah ekstrak daun singkong memiliki potensi sebagai antioksidan pada burung puyuh dewasa yang diberikan paparan panas singkat.

Gambaran Leukosit Setelah Pemberian Nanoenkapsulasi Andaliman (Zanthoxylum acanthopodium DC.) pada Burung Puyuh Pascainduksi Imunosupresan Deksametason Rasyida Ulfa; Akhiruddin Maddu; Huda Salahuddin Darusman; Koekoeh Santoso
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.295 KB)

The use of AGP (Antibiotic Growth Promoter) in animal has been banned because of the risk of antibiotic resistance. As an alternative for growth promoter. Andaliman (Zanthoxylum acanthopodium DC.) were used as feed additives. Andaliman is an herbal plant which proven has an immunomodulatory effect. However, herbal plants have low bioavailability in the body, so we need to increase its bioavaibility by reducing particle size using nanoparticle technology. The aim of our study was to determine the effects of three forms of andaliman as extracts, nanoparticles and nanoencapsulation on leukocyte differential, leukocyte count and Heterophil (H) / Lymphocyte.(L) ratio index. In this study we used 24 quails, which is divided into 4 groups, consist of.4 birds each. Group K was control group, birds were given dexamethasone (immunosuppressant), group P1 was given dexamethasone and andaliman extract, group P2 was given dexamethasone and andaliman nanoparticles, whilst group P3 was given dexamethasone and andaliman nanoencapsulation. The results showed that andaliman consumption had a significant effect (P <0.05) on differential leukocytes, number of leukocytes and H / L ratio after induction with immunosuppressants. Total leucocytes count based on the given treatment were K (8080 cell/mm3), P1 (20040 cell/mm3), P2 (20440 cell/mm3), and P3 (22040 cell/mm3). Each mean lymphocytes were K (44,6%), P1 (53,6%), P2(57,4%), and P3 (65,8%). Each mean heterophile were K (50,6%), P1 (41,8%), P2 (37,8%), and P3 (28,6%). Each mean monocytes were K (2,8%), P1 (4,6%), P2(4,8%), and P3 (5,0%). Each mean ratio index Heterophile (H) / Lymphocyte.(L) were K (1,1), P1 (0,7), P2(0,6), and P3 (0,5). It could be concluded that administration of Andaliman extract, Andaliman nanoparticles and Andaliman nanoencapsulation can increase the number of leukocytes, differential leukocytes and decrease the H / L ratio.

Co-Authors A.S. Satyaningtijas Abdul Zahid Abidah, Puri Adzrok ACHMAD FARAJALLAH Afiff , Usamah Afrilia Bagus Putri Hapsari Agik Suprayogi Agung Eru Wibowo Agus Wijaya Ainul Khadija Saleema Akhiruddin Maddu Aldo Febriano Tarigan Alfarisi, Hamzah Amalia Khairina Amrozi Andayani, Prawita Lintang Anindita Sista Widyadhari Anindita Sista Widyadhari Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Asah Hilaliah Athirah Rerana Fitrianthy Aziz, Sandra Dalia Sukmawati Damhuri Damhuri Damiana Rita Ekastuti Daryanti, Edhita Putri David Anwar Denny Widaya Lukman Denny Widya Hikman Desrayni Hanadhita Dhani S. Wibawa Dwi Nurhidayah Zubaidah Edi Sukmawinata Edi Sukmawinata Elok Budi Retnani Etih Sudarnika Farida Cintiyah Gandasari, Ira Agustina Dewi Gilang Kala Maulana, Gilang Kala Hadri Latif heny Nitbani HERA MAHESHWARI Herwin Pisestyani Huda Shalahudin Darusman I wayan Teguh Wibawan Ika Mayasari, Ni Luh Putu Ikhwan Wirahadikesuma ikhwan wirahadikesuma Imelda Yanti Lumongga Simanjuntak Irfan Nurhidayat, Irfan Irwan Oktoni Isdoni Bustaman Isnaeni Isnaeni Isnaeni Jefri Naldi Jumadin, La Jumardin jumardin jumardin Kholidah Fauziah Komariah komariah komariah Kudang Boro Seminar Kusnandar Anggadiredja La Jumadin La Jumadin La Jumadin La Jumadin Lili Darlian Lili Darlian M. Ichwan Mutaqqin MADE ASTAWAN Made Darawati Maharani Salsabila Mahendra, Arindina Maneewong, Sattabongkoch Maria, Joanita Maryce Agusthinus Walukou Mawali, Maharja Mokhamad Fakhrul Ulum, Mokhamad MUHAMMAD AGIL Muhammad Primadevan Pascaharchana Noor Nadhear Nadadyanha Dannar NASTITI KUSUMORINI Nenis Rahma Wulandari Niken Ulupi Nugraha, Arifin Budiman Nuri Ardiani Nurul Masyita, Nurul Okta Irviana Muminin Okti Nadia Poetri Ong Huey, Lynette Pudji Achmadi Putri, Anisya Saeila Putri, Fatimatus Sa’diyah Putri, Maritrana Rahmadhani, Elsi Rani Audona Rasyida Ulfa Ratasha Zahwanda Rachmani Ridi Arif Ridzky Pratama Risqika Akla Velayati Rivangga Yuda Hendika Ronald Tarigan Rotinsulu, Dordia Anindita Sadiah, Siti Santoso, Hera Sasmita, Annissa Nuridfi Satvika, Fadhila Saut Renato Rahmat Simanjuntak sri murtini . Supiyani, Atin Tarigan, Ronald Tutik Wresdiyati Ulfatin Khoiriyah Herowati Umi Cahyaningsih Warysatul Ummah Wasmen Manalu Wijaya, Yanuar Restu Yulvian Sani Yusuf Ridwan Zora, Nelda Fliza Zulvia Maika

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