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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biology, Medicine, & Natural Product Chemistry Biomedika Bioeksperimen: Jurnal Penelitian Biologi Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Majalah Kesehatan FKUB Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Herb-Medicine Journal MAGNA MEDICA: Berkala Ilmiah Kedokteran dan Kesehatan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat Al-Iqra Medical Journal : Jurnal Berkala Ilmiah Kedokteran Medfarm: Jurnal Farmasi dan Kesehatan Open Access Indonesian Journal of Medical Reviews Gemakes: Jurnal Pengabdian Kepada Masyarakat Proceeding ISETH (International Summit on Science, Technology, and Humanity) Prosiding University Research Colloquium Jurnal Pengabdian Masyarakat Medika Biomedika
Riandini Aisyah
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Proceeding ISETH (International Summit on Science, Technology, and Humanity)

 1 
Effectiveness of Purple Sweet Potato (Ipomoea batatas L.) Pressed Water as a Natural Coloring Agent for Examination of Feces Confirmed Helminthiasis Faatiha, Arsyal Wanda; Aisyah, Riandini; Dewi, Listiana Masyita; Rosyidah, Devi Usdiana
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5494

Abstract

Background: Helminthiasis is a parasitic disease caused by worms, with high prevalence in Indonesia ranging from 2.5% to 62%, depending on the region. Soil transmitted helminths are the most common causative of helminthiasis. The Gold Standard diagnose helminthiasis is microscopic examination using eosin. However, Eosin has the disadvantage that the waste from its use is not environmentally friendly and carcinogenic, prompting the search for natural dye alternatives. Purple sweet potato (Ipomoea batatas L.), rich in anthocyanins, was selected as a potential substitute because it contains higher anthocyanin levels compared to other plants. Aim: This study aimed to evaluate effectiveness of purple sweet potato pressed water as a substitute for eosin and to assess the differences between pure pressed water and with added citric acid in staining feces confirmed with helminthiasis. Methods: The study utilized a true experimental design with posttest-only control group approach. Purple sweet potato pressed water was tested at varying concentrations 5%, 10%, 15%, 20% and as well as at the same concentrations with the addition of citric acid. The population consisted of helminthiasis confirmed stool samples preserved in formalin. Staining effectiveness was assessed using Likert scale, and data were analyzed using Shapiro-Wilk, Levene, and Kruskal-Wallis tests. Results: Purple sweet potato pressed water without citric acid demonstrated good staining quality, result of Kruskal-Wallis p-values for coloring effectiveness (p=0.195), visualization of parasite structure (p=0.566), coloring quality (p=0.062). Conclusion: Purple sweet potato pressed water can be used as an alternative dye in helminthiasis examination. The addition of citric acid in the preparation can increase the stability of the formulation.
Effectivity of Rosella Flower Pressed Water (Hibiscus sabdariffa L.) as a Natural Coloring Agent for Examination of Feces Confirmed Helminthiasis Sulistyo, Ayu Indah; Aisyah, Riandini; Nurhayani, N; Mahmudah, Nur
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5497

Abstract

Background: Soil Transmitted Helminths (STH) are prevalent in tropics. Eosin 2% dye is commonly used for helminth egg identification, but this dye is carcinogenic and damaging to the environment. Aim: This study evaluates the effectiveness of rosella pressed water (Hibiscus sabdariffa L.) as a natural dye for helminthiasis feces examination, examines its performance with added citric acid, and compares the staining results. Methodology: This experimental study used a post-test only control group design with 27 purposively selected samples of 10% formalin-fixed helminthiasis feces. Staining was performed using rosella pressed water at concentrations of 10%, 20%, 30%, and 40%, with and without 9% citric acid, alongside 2% eosin as a comparison. Observations were made under a 10x microscope, and staining effectiveness was evaluated using a Likert scale by a parasitologist. Results: The staining quality of rosella pressed water was consistent (median = 3) across all concentrations. Parasite visualization varied without citric acid, with reduced quality at 20% concentration (median = 2). Preparation quality remained stable (median = 3). Kruskal-Wallis analysis showed no significant differences (p > 0.05). Discussion: Rosella flowers (Hibiscus sabdariffa L.) contain anthocyanin pigments which are flavonoid compounds that provide red to purple color, so they can be used as a natural dye for STH eggs. Conclusion: This study showed that rosella flower pressed water was effective for STH egg staining, although 2% Eosin gave the best and most consistent results. There were no significant differences between treatments.
Effectiveness of Mangosteen Peel Pressed Water (Garcinia mangostana L.) as an Organic Agent for Examination of Feces Confirmed Helminthiasis Rohmaniar, Delianti; Aisyah, Riandini; Bestari, Rochmadina Suci; Risanti, Erika Diana
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5501

Abstract

Purpose: This study aimed to compare the effectiveness of mangosteen peel pressed water without the addition of other ingredients with mangosteen peel pressed water (Garcinia mangostana L.) which has been added with citric acid as an organic agent in fecal examinations confirmed helminthiasis. This study is very relevant considering the global health problem of worms, which is often exacerbated by poor sanitation and traditional diagnostic methods that can pose health risks. Methodology: An experimental design was conducted with a post-test only group design. The study involved nine treatments and three repetitions, utilizing fecal specimens that tested positive for helminthiasis. The mangosteen peel pressed water was prepared in varying concentrations (10%, 20%, 30%, and 40%), both with and without citric acid. The effectiveness of the dye was assessed through various parameters, including staining quality and visualization of parasitic structures. Results: The analysis indicated that all concentrations of mangosteen peel pressed water demonstrated consistent effectiveness in staining. Statistical evaluation using the Kruskal Wallis test revealed no significant differences among the treatments, with p-values for effectiveness (0.645), visualization of parasitic structures (0.364), and quality of staining (0.463). This suggests that the different concentrations and conditions did not significantly impact the outcomes, confirming the reliability of mangosteen peel pressed water staining agent for feces examination. Applications: The findings support the use of mangosteen peel pressed water as a safe and effective alternative dye for detecting helminthiasis, leveraging its anthocyanin content. This research contributes to developing more environmentally friendly diagnostic methods, reducing reliance on potentially harmful chemicals used in traditional staining processes. By enhancing diagnostic accuracy, this study has the potential to improve community health outcomes in areas affected by helminthiasis, particularly in regions with limited access to safe diagnostic tools.
Correlation Parents' Knowledge and Environmental Sanitation Towards The Helminthiasis in Students Najmii, Muhammad Firoos Allaam; Bestari, Rochmadina Suci; Aisyah, Riandini; Rahmah, Maulida
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5521

Abstract

Purpose: The objective of this study is to determine whether parental knowledge about worms and environmental sanitation are related to the incidence of worms (helminthiasis) among students in SDN 02 Selokaton. Methodology: This study uses an analytical observational study research method with a cross-sectional research design. Results: there is no significant relationship between parental knowledge about worms and the incidence of worm infections, there is no significant relationship between environmental sanitation and the incidence of worm infections. Applications/Originality/Value: The novelty in this study is that no data has been found on the prevalence of worms in elementary school students in Karanganyar, so this study can be an initial screening of the prevalence of worms in elementary school students in Karanganyar.
Correlation of Length of Fever before Hospital Admission, Hematocrit, Platelet Count, and Leukocytes with Length of Hospitalization Dengue Virus Infection Patients in Children Pambudi, Nasrurrofiq Risvana Bayu; Puspitasari, Metana; Jatmiko, Safari Wahyu; Aisyah, Riandini; Alfajri, Asri
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5525

Abstract

Purpose: To analyze the relationship between fever duration before admission, hematocrit, platelet count, and leukocyte count with length of hospital stay in pediatric patients with dengue infection. Methodology: This observational study with cross-sectional design was conducted at PKU Muhammadiyah Sampangan Hospital, Surakarta, using secondary data from medical records. Forty pediatric patients were selected through consecutive non-probability sampling technique. Statistical analysis was performed with Spearman correlation test using statistical applications. Results: There was a significant association between fever duration before admission and length of hospitalization (r = -0.407, p = 0.009). However, there was no significant association between hematocrit (r = 0.136, p = 0.403), platelet count (r = 0.101, p = 0.536), or leukocyte count (r = 0.111, p = 0.496) with length of hospitalization. Applications/Originality/Value: This study highlights the importance of early detection and intervention in managing pediatric dengue cases. The significant correlation found between fever duration prior to admission and length of stay underscores the need for prompt treatment to minimize hospital stay due to associated inflammation in the body. In contrast, the lack of significant associations between hematocrit, platelet count, leukocyte level and duration of hospitalization highlights the complexity of factors influencing outcomes and the need to explore other prognostic markers. This study conducted in an endemic region of Indonesia contributes to understanding the management of dengue virus infection and underscores the importance of a comprehensive multifactorial approach to improve patient care strategies.
Effectiveness of Bit (Beta vulgaris L.) Fruit Pressed Water as A Natural Color on Examination of Fests Confirmed with Helminthiasis Wahyuni, Tri; Aisyah, Riandini; Bestari, Rochmadina; Kurniati, Yuni Prestyo
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5545

Abstract

Background: Helminthiasis is a significant public health issue, particularly in tropical and subtropical regions. The conventional staining method using 2% eosin, while effective, poses environmental and health concerns due to its carcinogenic properties and poor biodegradability. An alternative, natural, and environmentally friendly dye is necessary. Beetroot (Beta vulgaris L.), rich in betalain pigments, offers a potential solution. Aim: This study aims to evaluate the effectiveness of beet juice as a natural dye for microscopic examination of helminthiasis and assess its performance when combined with 9% citric acid. Methodology: A true experimental design with a posttest-only control group approach was employed. Beet juice concentrations of 70%, 80%, 90%, and 100% were prepared, both with and without 9% citric acid. Stool samples confirmed with helminthiasis were stained using these solutions and examined microscopically. The criteria assessed were effectiveness of coloration, visualization of parasite structure, and quality of staining results. Data were analyzed using the Kruskal-Wallis test. Results: Beet juice at all concentrations showed comparable effectiveness to 2% eosin across all parameters. Adding 9% citric acid enhanced the stability and longevity of the beet juice, maintaining color quality for up to 21 days, compared to 8 days without citric acid. Statistical analysis showed no significant differences between the staining results of beet juice and eosin (p > 0.05). Discussion: beetroot (Beta vulgaris L.) contains betasiani pigments and flavonoids which are antioxidants with an acidic pH that can color the protein layer of worm eggs which are alkaline in nature. Conclusion: Beet juice, particularly when combined with 9% citric acid, is a viable, natural alternative to 2% eosin for helminthiasis staining. It offers a safer and environmentally friendly option with comparable effectiveness and longer durability.
Co-Authors Abd Ghani, Mohd Faiz Ahmad Alrizaldi Alfajri, Asri Anam, Ilham Hafizha Maulana Angesti Atiqah Ranasatri Anggitaratri, Zakia Novi Anggreheni, Putri Dwi Anika Candrasari Anindita Putri Handayani Ariffah, Hilda Zaniba Arrohma, Farah Aryanto Asfarina, Fatin Ayyasi, Quanta Azenta, Moch. Tabriz Bestari, Rochmadina Brilliantama, Fandy Akbar Devi Usdiana Rosyidah Dwi Advina Herdiyanti Dyah Farida Inli Em Sutrisna Erika Diana Risanti Estu Puguh Prabancono Faatiha, Arsyal Wanda Fauzi, Favian Fauzi, Favian Arriella Shabri Ikmal Fikri, Muhammad Ihsanul Fitri, Alifah Aulia Graffico Eryza Oldiara Gunawan, Ryan Budi Haliza, Sheli Nur Handayani, Anindita Putri Herdiyanti, Dwi Advina Hidayah Karuniawati Hidayatullah, Muhammad Alim Abdul Majid Iin Novita Nurhidayati Mahmuda Indra Nuroso Jatmiko, Safari Kamila, Aulida Azkia Kumala, Aswa Arsa Kurniati, Yuni Prestyo Kusumaningrum, Tanjung Anitasari Indah Lestari, Nining Listiana Masyita Dewi Mapa, Anna Rochim Maulida Rahmah, Maulida Maulida, Amira Hasna Maylafaiza, Annisa Mitoriana Porusia Muhamad Irfan Hidayatullah Muhammad Adha Muhammad Alim Abdul Majid Hidayatullah Nafisa, Balqis Najmii, Muhammad Firoos Allaam NAJMII, MUHAMMAD FIROOS ‘ALLAM Noor Hafida Widyastuti Nordin, Noraziah Novita, Iin Nur Mahmudah Nur Mahmudah Nurhaliza, Sheli Nurhayani Nurhayani nurhayani Nurhayani, N Nuroso, Indra Pambudi, Nasrurrofiq Risvana Bayu Peni Indrayudha Prabancono, Estu Puguh Prabowo, Nurhasan Agung Pratama, Nur Puspitasari, Metana Rahayu, Destri Ramadhani, Marsyanda Ratnasari Bondan Wijayanti Reisya Tiara Kandita Reisya Tiara Kandita, Reisya Tiara Retno Sintowati - Rif'aini, Fidhia Nur Rochmadina Suci Bestari Rohman, Raihan Fadzlur Rohmaniar, Delianti Roslan, Nuruliza Ryan Budi Gunawan Ryanuranti , Rahma Ghaida Safari Wahyu Jatmiko Salsabila, Nabila Mutia Sella Felina Setiani, Hilda Sholeh, Muhammad Dzikru Irfan Sri Wahyuni Sri Wahyuni Sukardi, Nadya Sulistiyo, Azka Hafiy Sulistyo, Ayu Indah Supraba, Intan Pratiwi Suryani, Latifah Syahida, Safira Tara , Irmanawati Audhina Nirmala Tolibin, Restu Triwulandani Tri Agustina, Tri tri wahyuni Usdiana Rosyidah, Devi Utami, Aurelia Ratna Utami, Indari Wafiq, Muhammad Azzim Wahyu Jatmiko, Safari Wibowo, Haryo Kunto Wijayanti, Ratnasari Bondan Wulandari Berliani Putri Wulandari Berliani Putri, Wulandari Berliani