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Isolation and Antibacterial Activity Isolate of The Endophytic Fungi of Shallot Tubers (Allium cepa L.) Against Pathogen Bacteria Octaviani, Melzi; Jannah, Miftahul; Wulandari, Rizki; Utami, Rahayu; Fadhli, Haiyul
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 2, AUGUST 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

Shallots (Allium cepa L.) are widely known by the public as a kitchen spice and traditional medicine. Shallots have antibacterial activity. Compounds that have antibacterial properties can be obtained by isolating endophytic microbes. Endophytic microbes are microbes that live in plant tissue, are able to form colonies in plant tissue without having detrimental effects on the host, and can produce secondary metabolites like the host. Phytochemical screening of shallot tubers showed that they contained alkaloids, flavonoids, and phenolics. This research aims to isolate and identify endophytic fungi from shallot tubers and determine the antibacterial activity of these endophytic fungal isolates. Endophytic fungi were isolated using the direct planting method, and then pure isolates were identified macroscopically and microscopically. Antibacterial activity testing was carried out using the agar diffusion method against the bacteria Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, Escherichia coli, Salmonella typhi, and Shigella dysenteriae. There were 4 isolates of endophytic fungi produced during the isolation process, namely FEAC-U1 (Aspergillus), FEAC-U2 (Penicillium), FEAC-U3 (Trichophyton), and FEAC-U4 (Rhizopus). FEAC-U1 isolate showed the highest activity against Staphylococcus epidermidis, Bacillus cereus, and Shigella dysenteriae. FEAC-U2 isolate showed the highest activity against Salmonella typhi. FEAC-U3 isolate showed the highest activity against Staphylococcus aureus and Escherichia coli. The results of antibacterial activity testing on four isolates of shallot tuber endophytic fungi were able to inhibit the growth of the test bacteria.
Pencegahan anemia melalui edukasi dan pemberian suplemen di SMPN 1 kampar kiri hilir Fadhli, Haiyul; Pratiwi, Amanda Putri; Khoiriah, Anisah; Wulandari, Venny; Maspat, Rinta; Ananto, Deswita Sandra Tsalsabila
Yumary: Jurnal Pengabdian kepada Masyarakat Vol 6 No 1 (2025): September
Publisher : Penerbit Goodwood

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35912/yumary.v6i1.3702

Abstract

Purpose: The objective of this program is to improve adolescents' knowledge about anemia and raise awareness about the importance of a healthy lifestyle, particularly focusing on preventing stunted growth in the future. Methodology: The program was conducted at SMPN 1 Kampar Kiri Hilir. The methods used included lectures and discussions, with the support of leaflets for information dissemination. Additionally, pretest and posttest evaluations were conducted to measure the change in knowledge among the participants. A total of 94 students took part in the activity. Results: The program resulted in a significant improvement in students' knowledge about anemia, as shown by the increase in the average posttest score (85.11%) compared to the pretest score (61.7%). The socialization also raised awareness about the importance of taking blood supplement tablets to prevent stunting. Conclusion The socialization program was effective in improving adolescents' understanding of anemia, including its symptoms, causes, and prevention methods. Limitation: This study is limited to a single school with a homogeneous sample, without evaluating external factors or the long-term impact on behavioral changes and students' health status. Contribution: This program successfully improved students' knowledge about anemia through a school-based educational approach and supported efforts to prevent anemia and stunting among adolescent girls.
Pengolahan Sampah dalam Mendukung Perilaku Hidup Bersih Dan Sehat Di Sekolah Dasar Fadhli, Haiyul; Putri, Rusnita; Wanda, Taqqia Sajja; Maspat, Rinta; Asraf, Muhammad Arif
Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 1 (2026): Edisi Maret 2026
Publisher : FKIP Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36709/amalilmiah.v7i1.420

Abstract

Penerapan Perilaku Hidup Bersih dan Sehat (PHBS) pada anak usia sekolah dasar merupakan salah satu hal yang sangat penting dalam pencegahan risiko kesehatan akibat lingkungan yang tidak sehat. Program pengabdian masyarakat di sekolah dasar ini bertujuan mendukung penerapan PHBS dengan mengedukasi siswa tentang pengelolaan sampah yang efektif. Kegiatan dilakukan dengan metode ceramah interaktif dan diskusi, melibatkan 25 siswa kelas VI. Keberhasilan program peningkatan pengetahuan dievaluasi melalui pemberian pre-test dan post-test secara lisan. Hasilnya menunjukkan peningkatan yang signifikan dalam pemahaman siswa mengenai klasifikasi sampah, strategi pengelolaan sampah organik dan anorganik, serta dampaknya terhadap kesehatan lingkungan. Keberhasilan dari upaya ini tidak terlepas dari antusiasme para siswa dan relevansi materi dengan kehidupan sehari-hari mereka, sedangkan keterbatasan waktu menjadi kendala dalam mendalami materi lebih lanjut. Program ini berhasil meningkatkan kesadaran siswa akan pentingnya kebersihan dan kesehatan lingkungan serta membentuk mereka sebagai agen perubahan di sekolah. Ke depannya, program serupa diharapkan dapat diintegrasikan secara rutin di sekolah untuk memperkuat budaya PHBS serta didukung dengan fasilitas daur ulang guna memperkuat pembelajaran praktis.
Isolasi Senyawa Metabolit Sekunder dari Ekstrak Metanol Kulit Batang Bauhinia semibifida Roxb. dan Uji Aktivitas Antioksidan Haiyul Fadhli; Nur Fitria Rohmatul Ummah; Noveri Rahmawati
ALCHEMY Jurnal Penelitian Kimia Vol 17, No 1 (2021): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.17.1.42268.132-139

Abstract

Kulit batang Bauhinia semibifida Roxb. telah lama digunakan sebagai obat tradisional oleh masyarakat Melayu Lingga, Kepulauan Riau. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antioksidan senyawa metabolit sekunder dari ekstrak metanol kulit batang B. semibifida Roxb. Tiga isolat murni berupa satu senyawa alkaloid (BR1) dan dua senyawa flavonoid (BR2 dan BR3) telah berhasil diisolasi dengan cara ekstraksi menggunakan metode maserasi dan dilanjutkan fraksinasi dengan metode kromatografi kolom. Uji aktivitas antioksidan dilakukan terhadap isolat dengan metode 2,2 difenil-1-pikrilhidrazil (DPPH). Senyawa isolat BR1 menunjukan IC50 >1000 µg/mL dikategorikan tidak aktif sebagai antioksidan, sedangkan senyawa isolat BR2 dan BR3 menunjukan IC50 2,92 µg/mL dan 4,39 µg/mL dikategorikan sangat kuat sebagai antioksidan jika dibandingkan dengan vitamin C sebagai kontrol. Berdasarkan evaluasi aktivitas antioksidan senyawa BR2 adalah senyawa pilihan yang dilanjutkan identifikasi dengan spektroskopi Fourier-Transform Infrared Spectroscopy (FTIR) dan Proton Nuclear Magnetic Resonance (1H-NMR) sehingga diduga senyawa isolat memiliki struktur dasar senyawa flavonoid.Isolation of Secondary Metabolite Compounds of Methanolic Extract of Steam Bark Bauhinia semibifida Roxb. and Their Antioxidant Activites. Stem bark of Bauhinia semibifida Roxb. has been long consumed as a medicinal herb by Lingga Malaya Ethnic. Therefore, this research aimed to isolate and examines the antioxidant activity of secondary metabolites from methanol extract of stembark of Bauhinia semibifida Roxb. Three pure compounds which are an alkaloid (BR1) and two flavonoids (BR2 and BR3) have been isolated by extraction using maceration method followed by fractionation using column chromatography method. Antioxidant activity assay was carried out by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) method. Compound BR1 showed IC50 >1000 µg/mL categorized as inactive as an antioxidant, while compounds BR2 and BR3 showed IC50 2.92 µg/mL and 4.394 µg/mL, respectively, categorized as very strong as an antioxidant when compared to vitamin C as a control. Based on the evaluation of the antioxidant activity, the BR2 was selected compound and continued to identification by Fourier-Transform Infrared Spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy so that the isolate has the basic structure of flavonoid compound.
Penguatan Kesehatan Warga Desa Sungai Petai melalui Inovasi Jamu Instan, Infused Water dan Layanan Pemeriksaan Kesehatan Terpadu Haiyul Fadhli; Wira Noviana Suhery; Benni Iskandar; Silvana Anggraini; Taqqia Sajja Wanda; Rinta Maspat; deswita Sandra Tsalsabila Ananto; windi Alfayutia
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. 3 (2025): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.11.3.594-602

Abstract

Degenerative diseases such as hypertension, diabetes mellitus, and gout have developed and threaten the quality of life of the community, especially in rural areas. This community service aims to enhance awareness among the residents of Sungai Petai Village about the benefits of instant herbal medicine, infused water, and health education for early detection and maintaining a healthy lifestyle. The method used is service learning with health counseling, demonstration of instant herbal medicine and infused water, and health checks (blood pressure, blood sugar, and uric acid), followed by a pretest and posttest to assess the improvement of health education, with a pretest and posttest to determine the improvement of knowledge. The results showed that the education significantly improved participants' understanding of diseases and health products, with a considerable and sizable overall increase in knowledge at the posttest (80.83). The health check also showed that some participants had health conditions that required further attention.
Co-Authors Abdi Wira Septama Adel Zamri Adriani Susanty agistia, nesa Agustini, Tiara Tri Ainun Nurain Nurdin Ainun Nurain Nurdin Alfayutia, Windi Allaya, Regina Alpasiri, Ikhsan Mauludi Ananto, Deswita Sandra Tsalsabila Andika Bharada Rizky Soeharto Anggraeni, Ica Winanda Anita Lukman Apriko Jauwahir Arif Arsaf, Mohammad Armon Fernando Arsaf, Mohammad Arif Arsaf, Muhammad Arif Asraf, Muhammad Arif Azizah, Wahyu BENNI ISKANDAR Dachriyanus deswita Sandra Tsalsabila Ananto Devi Safitri Dewi, Erna Puspita Difa, Faradini Ramsanjami DINA FITRIYAH Dwi Winarsih Emrizal Enda Mora Erenda Yuneistya Erna Puspita Dewi Fadhila, Qonita Nur Fadillah, Ma’isyatul Fatma Sri Wahyuni Fatmalia, Anggun Fellysya, Nadya Safha Feriansyah, Robby Fina Aryani, Fina Fitriah, Dina Frasesa, Roscha Ulfa Furi, Mustika Ginta Rio Maranti Hazimah Hazimah, Hazimah Husnawati Husnawati Husnawati Ihsan Ikhtiarudin Iriani, Revy Islamia, Elmas Ghalis Nur Jasril Jasril Jumadila, Ikhsal Mukri Khoiriah, Anisah Maqfiroh, Wirdarani Mardianti, Tengku Shella Maspat, Rinta Meiriza Djohari, Meiriza Melzi Octaviani Miftahul Jannah Muhtadi, Wildan Khairi Musyirna Rahmah Nst Muti’ah, Try Hadirah Nadilla Alfitri Neni Frimayanti Ningsih, Yozi Fiedya Nofri Hendri Sandi Noveri Rahmawati Nur Fitria Rohmatul Ummah Nurul fadillah, Nurul Pedro Anugrah Aswan Pratiwi, Amanda Putri Pratiwi, Erniza Putra, Diova Yuswidia Putri Lestari Putri Lestari, Putri Putri Pratiwi, Amanda Putri, Rusnita Putri, Sella Hilmalia Nur Al Rahayu Utami Rahayu Utami Rahayu Utami Utami Rahima Rahma Dona Rahma Dona, Rahma Rahmatunnur, Siti Rahmayanı, Wıky Rahmiwati Hilma Ricardo, Nadira Atiqah Rifani, Mhd Rinta Maspat Rizki Wulandari Robiatun Adawiyah Rodhia Ulfa Ronapadua Musyirna Rahmah Nasution Sahara dan Emma Susanti Ruska, Shinta Liana Sandra Tsalsabila Ananto, Deswita Sari, Rinita Septi Muharni Silvana Anggraini Siregar, Lisa Andriyani Sitanggang, Sarah Dianora Sumbarita, Silvia Taqqia Sajja Wanda Trihardiyanto, Agus Tyasakti Viryana Ulfa Frasesa, Roscha Veithzal Rivai Zainal Viryana, Tyasakti Wanda, Taqqia Sajja Winda Tria Safitri Winda Yusma Ameliah Windarti Windarti windi Alfayutia Wira Noviana Suhery Wira Noviana, Wira Noviana Wulandari, Venny Yanwirasti Yanwirasti Yuneistya, Erenda Yuswidia Putra, Diova Zamri, Wiedya Alfitrya