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Effectiveness of Clove Flower Extract (Syzygium Aromaticum L.) as Analgesic on Licking Time Reaction in Male Mice with Formalin Induction Puspitasari, Vina; Wahjuni, Retno Sri; Saputro, Amung Logam; Hamid, Iwan Sahrial; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.226-230

Abstract

This research is about testing the effectiveness of clove flower extract (Syzygium aromaticum L.) as an analgesic against the beating time caused by formalin male mice. This research is experimental with the Complete Random Design formula producing four times repetition (N = 4) and divided into five treatment groups (t = 5). Data were analyzed using ANAVA factorial test then followed by Duncan test. All tests are carried out with Product Solutions and Service Statistics (SPSS). The results of research and statistical analysis prove that clove flower extract at a dose of 1% is an effective dose compared to other treatment groups, seen from the amount of time licking male mice  which is smaller than other treatment groups. It can be concluded that the administration of clove flower extract has analgesic effectiveness against the reaction of male mice induced by formalin. The concentration of clove flower extract 1% is the most effective in reducing licking time.
Gamal (Gliricidia Sepium) Leaf Extract Increase Mortality of Fasciola gigantica In Vitro Zulianti, Brillia; Hamid, Iwan Sahrial; Yudhana, Aditya; Rahmahani, Jola; Yunita, Maya Nurwartanti; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.193-198

Abstract

Fasciolosis is a disease caused by infection with liver fluke (Fasciola sp). Fasciolosis is a parasitic disease that is important because it can cause high economic losses. The aim of this study was to investigate the effect of gamal (Gliricidia sepium) leaf extract against mortality of Fasciola gigantica in vitro study. In present study was completely randomized design with six treatments and each treatment were divided into four replications. A total of 240 specimens from infected cattles were used in this study. The observation and recording of F. gigantica mortality in all treatments with concentrations of gamal leaf extract P1 (1%), P2 (2%), P3 (3), P4 (4%), K + (Nitroxynil 2%) and K- (physiological NaCl) rate was done in 30, 60, 90, 120, 150, 180, 210 and 240 minutes, respectively. The mortality of F. gigantica was confirmed if there was no movement when triggered by anatomy tweezers then dipped in slightly warm water (50 15í¢„Æ’"> ). The data was analyzed using ANAVA Faktorial and continued with Duncan Multiple Range Test. The results of this study were shown the lowest and the highest mortality rate recorded at minutes 30 and 240 respectively. Moreover, the mean of mortality rate were peaked in nitroxynil (K+) and 1% gamal leaf extract treatment, while the lowest mean of mortality rate occurs in NaCl (K-) treatment. Present study conclude that gamal leaf extract has anthelmintic effect on the mortality rate of Fasciola gigantica. Gamal leaf extract with 1% concentration was claimed to be the most effective.
Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi Wijaya, Achmad Arby; Hamid, Iwan Sahrial; Yunita, Maya Nurwartanti; Tyasningsih, Wiwiek; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.207-212

Abstract

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.
Effect of Robusta Coffee Extract on Histopathological in Mice Testes Induced with Monosodium Glutamat Plumeriastuti, Hani; Weliyani, Trima; Hamid, Iwan Sahrial; Hidanah, Sri; Prastiya, Ragil Angga; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.243-248

Abstract

Monosodium glutamate (MSG) is a sodium salt from glutamic acid which is currently very popular to be used as a food flavoring ingredient to stimulate appetite. Exessive consumption of MSG can disrupt the balance of antioxidants and ROS, and cause the negative effects of oxidative stress on testes. Coffee has high chlorogenic acid, function for antioxidants and reduce the negative effect on cell damage in the testes. This study aims to analyze the effect of robusta coffee extract on histological of mice testes induced with MSG. This study was completely randomized design (CRD), using 20 male mice for 5 treatment groups: K+ (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) orally for 42 days. Histophatological scores were analyzed with Saphiro-Wilk Test and Anova. There is no effect of increasing testicular weight. 0,12 mg MSG can cause a decrease in the number of spermatogonia and spermatocytes. 0,2 mg robusta coffee extract can maintain the number of spermatogonia and spermatocytes.
Risk Factors of Parvovirus in Dogs at De' Lab Pet Clinic, Bekasi Harelas, Genoveva Callista; Arimbi, Arimbi; Hamid, Iwan Sahrial; Praja, Ratih Novita; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.170-177

Abstract

This study aimed to determine the percentage of parvovirus, risk factor analysis, and describe the symptoms and treatment of parvovirus. The study was conducted by surveying, collecting secondary data from patient medical records and Bekasi City's rainfall data, data recording, data unification, and data analysis. Data analysis was presented descriptively and tested using Chi-Square and Odds Ratio (OR). The results shows the percentage of parvovirus was 2%. The rainfall factor <150mm/month significant to the risk of parvovirus (p<0,05) and increase the risk of parvovirus 0,154 times. The age factors 0-6 month significant to the risk of parvovirus (p<0,05) and increase the risk of parvovirus 0,017 times. The breed factors not significant to the risk of parvovirus (p>0,05) and don't increase the risk of parvovirus. The main symptoms seen in parvovirus include vomiting, diarrhea especially bloody diarrhea, and fever. The main components treatments are fluid therapy, antibiotic, antiemetic, and nutritional support. The administration of other drugs can be given to dogs whose administration must be adjusted to their respective conditions.
Total Plate Count of Broiler Meat at Various Market in Bekasi City in March 2021 Firdausyi, Aulia Amatul; Estoepangestie, Agnes Theresia Soelih; Wibawati, Prima Ayu; Hamid, Iwan Sahrial; Solikhah, Tridiganita Intan; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.157-161

Abstract

Food-safety of broiler meat is important because broiler meat is a foodstuff that can be easily contaminated by biological hazard such as bacteria. This study aimed to determine the total plate count of broiler meat at various market in Bekasi City in March 2021. Amount of samples in this study were 40 samples of broiler meat from 10 markets, samples were taken purposively and each sample used 25 grams from Musculus pectoralis of broiler meat. Samples were examined using pour plate method of total plate count. The result showed that 80% (32 of 40 samples) were exceeded the TPC's limit. The highest value of TPC result was 3,7 í— 107 CFU/g from "H” market.
Risk Factors and Incidence of Feline Lower Urinary Tract Disease (FLUTD) in Sahabat Satwa Genteng Animal Clinic Plumeriastuti, Hani; Novitasari, Seline Putri; Arimbi, Arimbi; Yunita, Maya Nurwartanti; Hamid, Iwan Sahrial; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.15-20

Abstract

The purpose of this study was to determine the risk factors and incidence of Feline Lower Urinary Tract Disease (FLUTD) at the Sahabat Satwa Genteng clinic. A total of 36 samples were evaluated according to research procedures with diagnosis, data collection, urine sampling, dipstick and sediment examination methods, and Chi-square data analysis. The results showed that there was a association between gender, history of castration, and diet on the risk of FLUTD. Meanwhile, there was no association between age and the risk of FLUTD. Clinical signs that often occur in FLUTD disease initiate from stranguria, hematuria, dysuria, periuria, and polykisuria. The results of the dipstick examination founded that 86,1% of cats affected by FLUTD had a normal pH and 13,9% had an alkaline pH. The results of the specific gravity (SG) of cats with FLUTD have values below normal while the protein levels found in all cats suffering from FLUTD have values above normal. Stranguria was found in 69,4% of cats with FLUTD. Leukocytes were also found in all the urine of cats suffering from FLUTD. The most common results of sediment examination were struvite, calcium oxalate, and cystine crystals.
PENGARUH PEMBERIAN EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL YANG DIINDUKSI GENTAMISIN PADA TIKUS (Rattus norvegicus) Nugrahani, Anggi Dwi; Nangoi, Lianny; Meles, Dewa Ketut; Widiyatno, Thomas Valentinus; Hamid, Iwan Sahrial; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19989

Abstract

This research was aimed to know the effect of leaves extract of sambiloto as a nephroprotector on renal histopathology features induced by gentamicin in white rats (Rattus norvegicus). The effect of leaves extract of sambiloto was tested in 25 rats. Twenty five male white rat were randomly divided into five groups; K- as negative control was given carboxy methyl cellulosa in 15 days, P0 was given carboxyl methyl cellulosa in 15 days, P1 was given leaves extract of sambiloto of 177 mg/kg bw/day/po in 15 days, P2 was given leaves extract of sambiloto of 189 mg/kg bw/day/po in 15 days, P3 was given leaves extract of sambiloto of 239,5 mg/kg bw/day/po in 15 days. On the 15 day of experimental, P0, P1, P2, P3 was given 200 mg/kg bw of gentamicin. Gentamicin solutions on P0, P1, P2 and P3 treated through intramuscular injection. After 24 hours of gentamicin induced, take the rats renal by laparotomy and then making preparations histopathology with staining Haematoxylin-Eosin (HE). Based on the result of Kruskall-Wallis statistical analysis on renal histopathology showed this research that the treatment have significant difference (p < 0,05). The leaves extract of sambiloto treated group (P3) gave the best value on the renal morfology, it gave a good effects in preventing nephrotoxicity in white rats.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP PENURUNAN KADAR MALONDIALDEHID (MDA) PARU MENCIT JANTAN (Mus musculus) YANG DIPAPAR ASAP ROKOK Dewi, Sapna Kurnia; Lokapirnasari, Widya Paramitha; Meles, Dewa Ketut; Mustofa, Imam; Mafruchati, Maslichah; Hamid, Iwan Sahrial
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20416

Abstract

This research aimed to determine the effect of kepok banana peel extract (Musa acuminata) given by per oral in decreasing levels of malondialdehyde (MDA) in lung of male mice (Mus musculus) which was exposed to cigarette smoke. The 24 experimental male mice were divided into 6 groups. The therapy was given for 14 days, all mice were sacrificed and lung MDA level were examined with thiobarbituricacid (TBA) methods. The result of the data analysis test using one-way ANOVA (Analysis of Variance) showed that there were significant differences between the group of animals that exposed to cigarette smoke ( = 840.50± 186.47 nmol / g) and the group of animals that were given with extract of kepok banana peel 56 mg / kg bw  ( = 6471.25 ± 69.25 nmol/g). Besides that, there was an significant difference between the groups of animals that were exposed cigarette smoke ( = 840.50± 186.47 nmol / g) with the group of animals that were given with vitamin C 260 mg/kg bw ( = 555.25± 61.16 nmol / g). It could be concluded that extract of kepok banana peel and vitamin C could decrease MDA levels in lung of male mice which was exposed cigarette smoke. The increased therapy dose gave affect in lowering levels of malondialdehyde in lung.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.
Co-Authors A.A. Ketut Agung Cahyawan W Abdul Samik, Abdul Aksono HP., Eduardus Bimo Annise Proboningrat Ardy, Carolina Arianto, Intan Diah Safitri Arimbi Budi Utomo Chairul Anwar Dermayati, Cyntia Zain Dermayati, Cyntia Zain Desy Fatmawati Dewi, Sapna Kurnia Dwi, Welyanto Kurniawan E Bimo Aksono Erma Safitri Estoepangestie, Agnes Theresia Soelih Fachrur Rozi Faisal Fikri Fedik Abdul Rantam Ferdiansyah, Muhammad Billy Firdausyi, Aulia Amatul Fransiska, Eunike Melanda Hani Plumeriastuti Harelas, Genoveva Callista Hermadi, Herry Agus Hestiana, Eka Pramyrtha Hidanah, Sri IMAM MUSTOFA Ira Sari Yudaniayanti Ivanalee, Angela Swasti Jeanita, Winny Kadek Rachmawati Kariyaningtias, Vivi Kartikasari, Anjani Marisa Kresnamurti, Angelica Kusumaningrum, Putri Anggraheni Lake, Werenfridus Kono Lastoaji, Firman Lianny Nangoi Lokapirnasari, Widya Paramitha Lovela, Aprinda Ratna Lucia Hendriati Luqman, Epy Muhammad Mafruchati, Maslichah Mardatillah, Meidy Meles, Dewa Ketut Muhammad, Abdul Latif Mustofa Helmi Effendi Muzhaffar, Adam Maulana Novitasari, Seline Putri Nugrahani, Anggi Dwi Nusdianto Triakoso octavia, Arinda Praja, Ratih Novita Pramesti, Nugraheni Anggara Prastiya, Ragil Angga Primarizky, Hardany Puspitasari, Vina Putrianirma, Rizki Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Rasdianto, Dwi Damayanti Ratna Damayanti Retno Bijanti Salahuddin, Muhammad Soleh Santoso, Kuncoro Puguh Saputro, Amung Logam Sarudji, Suryanie Satria, Rafif Galih Solikhah, Tridiganita Intan Sri Mulyati Sri Pantja Madyawati, Sri Pantja Sucita, Ricky Eka Suherni Susilowati Sukmanadi, Mohammad Sunarso, Agus Supriyadi Supriyadi Suresti, Suresti Surya, Regiskha Hermandanie Suwarno Suwarno Tatik Hernawati Teguh Widodo Tito, Muhammad Tjuk Imam Restiadi Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahjuni, Retno Sri Wahyudi, Alif Ekacahya Weliyani, Trima Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Wijaya, Achmad Arby Willyanto, Jennifer Rachel Wilujeng, Estiary Wurlina, W Yudhana, Aditya Yunita, Maya Nurwartanti Yuseni Kusuma P Yustinasari, Lita Rakhma Zahro, Vida Seanita Zulianti, Brillia