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All Journal Media Kedokteran Hewan Indonesian Journal of Cancer Chemoprevention Jurnal Medik Veteriner JFIOnline Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) journal of Basic Medical Veterinary Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Iwan Sahrial Hamid
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health Veterinary

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Factors for Increasing Goat Productivity with Probiotic Supplements in Kalipuro and Pesanggaran, Banyuwangi Hamid, Iwan Sahrial; Fikri, Faisal; Tito, Muhammad
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.45710

Abstract

Goat farming is an economic sector asset to support food availability and security in Banyuwangi. The existence of livestock is mainly to be supported by the adequacy of feed in order to increase productivity. This study is to evaluate the factors that play a role in increasing livestock productivity by adding probiotics to feed. The method used is surveys and questionnaires to farmers to evaluate farmers' understanding of feed quality. The results of 45 farmers as respondents, reported factors of knowledge (p<0.000), age (p<0.000), and application of feed (p<0.000) which significantly affect livestock productivity. It can be concluded that the level of knowledge, age, and application of feed are crucial factors for farmers to increase livestock productivity.
Duodenum Histomorphology of Bacillus subtilis and Bacillus coagulan Isolate in Laying Hen Strain Isa Brown Muzhaffar, Adam Maulana; Hamid, Iwan Sahrial; Effendi, Mustofa Helmi; Wibawati, Prima Ayu; Sukmanadi, Mohammad; Solfaine, Rondius
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.48030

Abstract

Probiotics are live microbes that are used as supplementary feed that work to increase the good bacteria that live in the digestive tract by suppressing pathogenic microorganisms in the intestine so that good bacteria can grow can optimize the feed absorption process. This study aims to determine the effect of adding probiotics as feed additive Against the height and width of the duodenal villi of the laying chicken in early production. Probiotics can nourish the digestive tract and increase nutrient digestibility so that nutrient intake is met for livestock. Bacillus subtilis and Bacillus coagulans as a prophylaxis against bacterial diseases by balancing intestinal microbes, increasing digestibility, and secreting protease, lipase, and amylase enzymes. The purpose of this study was to determine the effect of probiotics Bacillus subtilis and Bacillus coagulans on duodenal histomorphology. Twenty-four layers were randomly divided into 4 groups. The control group was not given probiotics, groups P1, P2, and P3 were given probiotics of 2 ml, 4 ml, and 6 ml. Probiotics were given for 14 days. One-way ANOVA showed a significant difference in each group (P<0.05) and continued with the DUNCAN test. The average length and width of the duodenal villi in each treatment showed that the control group was significantly different from the P1, P2, and P3 groups. The P3 group had the highest average length of duodenal villi. The increase in villi height and villi width is due to the increase in short-chain fatty acids induced by probiotics. An increase in the height and width of villi indicates the absorption capacity of the small intestine, the work of digestive enzymes on the intestinal mucosa is more maximal in the intestine with a wider morphological size, so that absorption and growth are better. The conclusion from the research that has been done is that the use of Bacillus subtilis and Bacillus coagulans as probiotics has an effect on the layer duodenal villi which is characterized by an increase in the average length of the duodenal villi according to the increase in the probiotic dose.
Curative Properties of Kencur (Kaempferia galanga L.) Extract on Mice (Mus musculus) Kidney Histopathology Exposed to Cigarette Smoke Arianto, Intan Diah Safitri; Lastoaji, Firman; Sudjarwo, Sri Agus; Valentinus, Thomas Widiyatno; Hestiana, Eka Pramyrtha; Hamid, Iwan Sahrial; Herupradoto, Eduardus Bimo Aksono; Lovela, Aprinda Ratna
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i1.2025.30-39

Abstract

The aim of this research was to investigate the therapeutic effects of Kencur (Kaempferia galanga L.) extract on kidney histopathological damage in male mice exposed to cigarette smoke. Twenty 8-week-old male mice were randomly divided into five treatment groups (n=5). The negative control group (C-) consisted of mice not given kencur extract or exposed to cigarette smoke, while the positive control group (C+) comprised mice exposed to cigarette smoke without kencur extract. Treatment groups included Treatment 1 (T1) administered 150 mg/kg BW of kencur extract, Treatment 2 (T2) administered 300 mg/kg BW, and Treatment 3 (T3) administered 600 mg/kg BW. Mice were exposed to cigarette smoke for 14 days. The data were analyzed using the Kruskal-Wallis test to evaluate overall differences among the treatment groups. Where significant differences were found (p<0.05), further pairwise comparisons were conducted using the Mann-Whitney test, confirming statistically significant differences between specific treatment groups.  Treatment 3 (T3) was found to be the most effective in reducing histopathological damage in the kidneys of male mice. This study demonstrates that kencur extract effectively reduces histopathological damage in the kidneys of mice exposed to cigarette smoke, highlighting its potential as a protective agent against smoke-induced tissue injury.
Effects of Colostrum Probiotics on ACE-2 Expression and Hematology in Murine Models Immunized Against Canine Coronavirus Hamid, Iwan Sahrial; Solfaine, Rondius; Supriyadi, Supriyadi
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.343-349

Abstract

In modern society, the human-animal bond has significantly strengthened, with pets evolving into integral family members providing psychological support to their owners. In the current landscape of heightened focus on immune health and the widespread utilization of probiotic advancements globally, this study investigates the potential of colostrum probiotics to influence hematological profiles and ACE-2 expression in a murine model of canine coronavirus (CCV) infection. Twenty-four mice were categorized into four treatment groups: (C) placebo, (T1) CCV induction + isoprinosine, (T2) CCV induction + probiotic-based, and (T3) CCV induction + colostrum-based probiotics. CCV induction was performed via subcutaneous injection of a live-attenuated CCV vaccine at 60 mg/kg body weight for 7 consecutive days. Probiotics were administered via oral gavage for 14 consecutive days. On the 15th day post-treatment, euthanasia was performed, and blood samples were collected to examine hematological profiles and ACE-2 enzymes in the intestinal tissue sections. Data analysis, conducted using ANOVA followed by Duncan's test (p < 0.05), revealed a significant improvement in the T3 group, particularly in leukocytes, hemoglobin, lymphocytes, neutrophils, and ACE-2 expression. In conclusion, this study suggests that probiotics, specifically colostrum-based, enhance immune parameters and ACE-2 expression in the intestinal milieu of murine model subjected to CCV immunization.
Uji Antipiretik Patch Ekstrak Etanol Bawang Merah (Allium ascalonicum L.) dengan Matriks Chitosan dan Enhancer Tween-80 Willyanto, Jennifer Rachel; Hamid, Iwan Sahrial; Widodo, Teguh
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 5 No. 1 (2018)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v5i1.2056

Abstract

Onion is a traditional medicine that has a lot of pharmacological potential. Flavanoid compounds found in onions have an antipyretic effect that acts as an inhibitor of cyclooxygenase (COX) enzymes that play a role in the formation of prostaglandins. In this study red onion is formulated into a patch because of its practical use and can avoid the first metabolic pathway. This study aims to analyze the effect of onion ethanol extract patch (Allium ascalonicum L.) on the decrease of body temperature and the number of monocytes in white rats induced by DPT vaccine. This study used 4 treatment groups: positive controls administered paracetamol syrup, negative controls given placebo patch, treatment group 1 treated with red onion ethanol extract without enhancers and treatment group 2 treated with an red onion ethanol extract with enhancers. Rats adapted for 7 days before temperature being measured and the blood being collected from the rat tail in order to calculate the number of monocytes. The animal temperature was measured after injected with vaccine DPT at 15, 30, 45, 60, 75, 90, 105, 120, 150, 180 minutes meanwhile number of monocyte were calculated every 15 minutes for 120 minutes. The results showed that there was no significant difference in monocyte count of treatment group with positive control but there were significant differences in temperature. The use of onion ethanol extract patch may decrease the temperature and number of monocytes in white mice induced by DPT vaccine.
Uji Antipiretik Patch Ekstrak Etanol Bawang Merah (Allium ascalonicum L.) dengan Matriks Kitosan dan Enhancer SPAN-80 terhadap Temperatur dan Jumlah Makrofag pada Tikus Putih Kariyaningtias, Vivi; Hamid, Iwan Sahrial; Widodo, Teguh
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 5 No. 2 (2018)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v5i2.2141

Abstract

Traditional medicine can be used to treat various diseases, one of which is onion (Allium ascalonicum L.) as an antipyretic agent. In this study, the antipyretic potency of ethanol extract of onion (Allium ascalonicum L.) was tested using chitosan and Span-80 as enhancer matrix with temperature and number of intraperitoneal macrophages in white rats as parameters. This study used 20 male white rats Wistar that were induced with DPTHb vaccine intraperitoneally and then divided into four groups namely positive control group (paracetamol), negative control (placebo without extract and without enhancer), formulation 1 (with extract and without enhancer), and formulation 2 (with extracts and enhancers). The results were analyzed using One Way Anova and if the results were significant, then the test continued with Duncan 5%. Correlation calculations were used to determine the relationship between temperature reduction and total intraperitoneal macrophages. The results showed that the temperature reduction did not provide a significant difference between the positive control (paracetamol) and formulation 2 (with extracts and enhancers), whereas in the negative control (without extract and without enhancer) there was no significant difference with formulation 1 (with extract and without enhancers). The total results of intraperitoneal macrophages were significantly different in all groups with a significant> 0.05.
Identifikasi Salmonella sp. pada Susu Segar di Peternakan Sapi Perah Kecamatan Licin Kabupaten Banyuwangi Satria, Rafif Galih; Hamid, Iwan Sahrial; Wibawati, Prima Ayu; Estoepangestie, Agnes Theresia Soelih; Saputro, Amung Logam; Praja, Ratih Novita
Media Kedokteran Hewan Vol. 32 No. 3 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i3.2021.114-118

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui tingkat cemaran bakteri Salmonella sp. pada susu segar dari peternakan sapi perah di kecamatan licin kabupaten banyuwangi. Sampel susu segar di ambil pada april 2021 sebanyak 16 sampel dari KPSP Ijen makmur di kecamatan Licin Kabupaten Banyuwangi. Sampel diambil sebanyak 25 ml dari masing-masing peternak. Hasil yang di dapat setelah melakukan isolasi dan identifikasi menggunakan media Salmonella Shigella Agar (SSA), dan dilanjutkan uji identifikasi menggunakan TSIA dan Urea didapatkan  dari ke 16 sampel semua dinyatakn negatif bakteri Salmonella sp.. Hal ini dapat terjadi karena para peternak sapi perah di Kecamatan licin sudah mulai menyadari pentingnya kebersihan kandang dan sanitasi kandang yang baik sehingga tingkat cemaran bakteri Salmonella sp. dapat dicegah.
Cytotoxicity of Kembang Bulan (Tithonia diversifolia ) Ethanolic Leaf Extract on Rabbit Limbal Mesenchymal Stem Cells Plumeriastuti, Hani; Zahro, Vida Seanita; Triakoso, Nusdianto; Rantam, Fedik Abdul; Hamid, Iwan Sahrial; Effendi, Mustofa Helmi; Proboningrat, Annise
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.233-239

Abstract

Kembang Bulan (Tithonia diversifolia ) is known as an herbal plant that has many useful properties to cure disease, such as diabetes, malaria, and other infections. Instead of much usefulness, T. diversifolia has main biological properties that may induce toxicity in this plant known as sesquiterpene lactones. The aim of this study was to identify the cytotoxicity effect of T. diversifolia leaf extract on limbal mesenchymal stem cells of rabbits using the MTT assay. T. diversifolia leaf extract was divided into 4 concentrations (0.5%, 0.25%, 0.125%, and 0.0625%) and given to limbal mesenchymal stem cells of rabbits in 96-well microplates, which would be incubated for 24 hours. The cytotoxicity result was obtained using the MTT assay method. Every well would be observed to see the cell life level of each concentration. The optical density absorbance was calculated using a microplate reader. T. diversifolia leaf extract with a concentration of 0.0625% has the biggest viability with a level of existence of limbal mesenchymal stem cells of 50%, and the other T. diversifolia leaf extract concentrations of 0.5%, 0.25%, and 0.125% have less than 50% level of existence of limbal mesenchymal stem cells.
Effect of the Extract of Dayak Onions (Eleutherine palmifolia) on the Sperm Quality of Mice (Mus musculus) Induced with Monosodium Glutamate Rahmatullah, Aldin Akbar; Sugihartuti, Rahmi; Susilowati, Suherni; Hamid, Iwan Sahrial; Suprayogi, Tri Wahyu; Rachmawati, Kadek; Safitri, Erma; Mustofa, Imam; Mulyati, Sri
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.113-122

Abstract

This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the motility, viability, and concentration of spermatozoa in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C- (CMC-Na 0.5%), C+ (4 mg/g BW of MSG), T1 (4 mg/g BW of MSG and 30 mg/kg BW of Dayak onion extract), T2 (4 mg/g BW of MSG and 60 mg/kg BW of Dayak onion extract), and T3 (4 mg/g BW of MSG and 120 mg/kg BW of Dayak onion extract). All treatments were administered for 52 days. The results showed no significant differences in the motility and concentration of spermatozoa between the C- (81.50 ± 4.18; 7.21 ± 0.37) and T3 (77 ± 2.74; 7.04 ± 0.13) groups (p < 0.05). In addition, the results showed significant differences in the viability of spermatozoa between the C- (83.30 ± 3.40) and T3 (77.20 ± 4.06) groups. Therefore, it can be concluded that the administration of Dayak onion extracts orally to mice induced with MSG can maintain the motility, viability, and concentration of spermatozoa.
Co-Authors A.A. Ketut Agung Cahyawan W Annise Proboningrat Ardy, Carolina Arianto, Intan Diah Safitri Arimbi Chairul Anwar Dermayati, Cyntia Zain Dermayati, Cyntia Zain Desy Fatmawati Dewi, Sapna Kurnia Dwi, Welyanto Kurniawan E Bimo Aksono Erma Safitri Estoepangestie, Agnes Theresia Soelih Fachrur Rozi Faisal Fikri Fedik Abdul Rantam Firdausyi, Aulia Amatul Fransiska, Eunike Melanda Hani Plumeriastuti Harelas, Genoveva Callista Herupradoto, Eduardus Bimo Aksono Hestiana, Eka Pramyrtha Hidanah, Sri IMAM MUSTOFA Ira Sari Yudaniayanti Ivanalee, Angela Swasti Jeanita, Winny Kadek Rachmawati Kariyaningtias, Vivi Kartikasari, Anjani Marisa Kresnamurti, Angelica Kusumaningrum, Putri Anggraheni Lake, Werenfridus Kono Lastoaji, Firman Lianny Nangoi Lokapirnasari, Widya Paramitha Lovela, Aprinda Ratna Lucia Hendriati Luqman, Epy Muhammad Mafruchati, Maslichah Meles, Dewa Ketut Muhammad, Abdul Latif Mustofa Helmi Effendi Muzhaffar, Adam Maulana Novitasari, Seline Putri Nugrahani, Anggi Dwi Nusdianto Triakoso Praja, Ratih Novita Pramesti, Nugraheni Anggara Prastiya, Ragil Angga Puspitasari, Vina Putrianirma, Rizki Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Rasdianto, Dwi Damayanti Ratna Damayanti Restiadi, Tjuk Imam Retno Bijanti Salahuddin, Muhammad Soleh Santoso, Kuncoro Puguh Saputro, Amung Logam Sarudji, Suryanie Satria, Rafif Galih Solikhah, Tridiganita Intan Sri Mulyati Sucita, Ricky Eka Suherni Susilowati Sukmanadi, Mohammad Supriyadi Supriyadi Suresti, Suresti Surya, Regiskha Hermandanie Suwarno Suwarno Tatik Hernawati Teguh Widodo Tito, Muhammad Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahjuni, Retno Sri Wahyudi, Alif Ekacahya Weliyani, Trima Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Wijaya, Achmad Arby Willyanto, Jennifer Rachel Wilujeng, Estiary Yudhana, Aditya Yunita, Maya Nurwartanti Yuseni Kusuma P Yustinasari, Lita Rakhma Zahro, Vida Seanita Zulianti, Brillia