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All Journal Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Hemera Zoa Jurnal Sain Veteriner Jurnal Ilmu Komputer dan Informasi Jurnal Veteriner Majalah Kedokteran Bandung Jurnal Ilmu Ternak Veteriner Jurnal Media Gizi Indonesia (MGI) INDONESIAN JOURNAL OF PHARMACY Pharmascience Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry AcTion: Aceh Nutrition Journal Jurnal Medik Veteriner Amerta Nutrition Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Farmamedika (Pharmamedica Journal) Jurnal Dunia Gizi Narra J Journal of Health and Nutrition Research Journal of Global Pharma Technology Jurnal Kedokteran Hewan Current Biomedicine Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research)

Ekowati Handharyani

Departemen Klinik Reproduksi Patologi, Fakultas Kedokteran Hewan, IPB University, Kampus IPB Dramaga Bogor, 16680, Indonesia

Author-ID : 274303

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Deteksi Coxiella burnetii pada Sapi Kurban Idhul Adha di Cimanggu, Kabupaten Bogor Tahun 2015-2016 (DETECTION OF COXIELLA BURNETII IN IED AL-ADHA SACRIFICIAL COW IN CIMANGGU, BOGOR REGENCY AT 2015-2016) Handayu Untari; Agus Setiyono; Ekowati Handharyani; Masdiana C. Padaga
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.846 KB)

Coxiella burnetii is an intracellular obligate bacterium that causes Q fever disease. Q fever is one of the asymptomatic zoonosis and has a potential as biological weapon. This disease infects much type of animals and has been distributed globally, including in Indonesia. Previous research concluded that West Java is an endemic area of this disease. Bogor in West Java has a potential spread of Q fever disease due to high population of cattle and goat in this region.This study aim was to detect C. burnetii in spleen, lung, kidney, liver and heart of cows. Samples of were taken from a total of 29 sacrificial cows during Eid al-Adha in 2015 and 2016 in the Cimanggu region, Bogor Regency. The examination method used was immunohistochemistry with primary polyclonal antibody Rabbit anti-C. burnetii and HaematoxylineEosine staining. The results of the immunohistochemistry examination showed positive immunoreaction (specific brown color in the cytoplasm of cells) against C. burnetii antibodies respectively in 3 out of 10 samples in 2015 (30%) and 4 out of 19 samples in 2016 (21%). Out of 29 samples, positive immunoreaction were found in lung organ (13,7%) and spleen (10,3%) with variation in changes in histopathological features which include congestion, inflammatory cell infiltration, mucus accumulation, and fibrosis in the lung.

Deteksi Virus Avian Influenza H5N1 pada Anak Ayam Umur Satu Hari dengan Teknik Imunohistokimia Sophia Setyawati; Retno Damajanti Soejoedono; Ekowati Handharyani; Bambang Sumiarto
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian Influenza (AI) or bird flu caused by virus H5N1 is still present in Indonesia. The Department ofAgriculture of Indonesia has banned poultry distribution from endemic to non endemic area, except fordistribution of day old chick (DOC). The aim of this research was to detect AI virus infection in DOCdistributed from AI endemic to AI non endemic areas. Two hundred and forty DOCs from farms in WestJava and Banten were collected from Soekarno Hatta airport. Their antibody titers were examined againstAI virus by Haemaglutination Inhibition (HI) test. The AI virus detected in tissues (trachea, lung, heart,kidney, liver, and intestine) by immunohistochemistry technique. Detection of AI virus using anti AI H5N1monoclonal antibody was conducted AEC as chromogen. The result showed that 66,2% of DOC were positiveAI and 33,8% were negative AI. The 66,2% of positive samples, 43,3% showing the presence of AI antigenin trachea, lung and intestine, and 22,9% were presence in liver and kidney. DOCs were infected AI viruswith subclinical symptoms and they were potential as the source of rapid AI spread in Indonesia. It istherefore important to take a very cautious measure to prevent the spread of AI via DOC from AI endemicto free area.

Aktivitas Hepatoprotektor Ekstrak Metanol Akar Pasak Bumi dan Fraksi-Fraksi Turunannya Ruqiah Ganda Putri Panjaitan; Wasmen Manalu; Ekowati Handharyani; Chairul -
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The hepatoprotector activity of pasak bumi (Eurycoma longifolia Jack.) roots was evaluated in carbontetrachloride (CCl4)-induced rats. Each rat was administered 500 mg/kg body weight of metanol extractand its derivates (n-hexane, chloroform, ethyl acetate, and methanol-water) for 7 consecutive days. Thepositive control group received 25 mg silymarin/kg body weight daily for 7 consecutive days. Hepatoprotectoractivity was assessed by measuring serum alanine transaminase (ALT) and aspartate transaminase(AST). Futhermore, hepatic tissues were subjected to histopathological studies. The results and AST(272.33±85.63 U/L) compared to methanol extract (380.61±324.88 U/L and 475.27±412.31 U/L), n-hexanefraction (279.80±304.92 U/L and 488.53±426.81 U/L), chloroform fraction (418.30±294.80 U/L and427.70±273.08 U/L), and ethyl acetate fraction (312.80±443.30 U/L and 418.40±370.52 U/L), but thisresult was not as good as silymarin (ALT 105.09±21.62 U/L and AST 310.25±2.45 U/L). Moreover, thehistopathological studies of methanol-water fraction was also not as good as silymarin . It was concludedthat methanol-water fraction of pasak bumi roots has a hepatoprotector potensial.

STUDI HISTOPATOLOGI PADA LIMPA DAN BURSA FABRICIOUS AYAM NEWCASTLE DISEASE DARI KASUS LAPANG HISTOPATHOLOGY (STUDIES ON SPLEEN AND BURSA OF FABRICIUS OF NEWCASTLE DISEASE CHICKHENS FROM FIELD CASE) Etriwati Etriwati; Ekowati Handharyani; Surachmi Setiyaningsih
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Newcastle Disease is a highly contagious and very virulent avian disease in all of poultry. The aims of this study were to determine the lesions and distribution of Newcastle disease virus on spleen and bursa of Fabricous of Newcastle disease chickhens from field cases. The sample used in this study were the spleen and bursa Fabricious organ of ten cases of suspect Newcastle disease. Haematoxylin and eosin staining examination was performed determine of cell morphology and distribution of the virus by immunohistochemical staining. The distribution of the lesions and the degree of reaction were determined by the category immunopositive mild, moderate and severe. Gross lesion on spleen, including swollen, fragile and necrotic multifocal. Histopathological lesions showed hyperemia/congestion and depletion of lymphoid cells. Immunopositive reaction found in lymphoid cells of red pulp and lymphoid cells in lymphoid follicles. Bursa of Fabricious showed atrophy and hyperemia/congestion with histopatological lesions hemorrhages, lymphoid cells in lymphoid follicles undergo necrosis and depletion of lymphoid follicles. Immunopositif reaction was observed in lymphoid cells in the mantle zone of lymphoid follicles. Newcastle disease from field cases cause damage to the spleen and bursa of Fabricius with degrees lesions and virus distribution in the organs are severe.

Blood Profile of Domestic Cat (Felix catus) During Skin Graft Recovery with Different Period (PROFIL DARAH KUCING LOKAL SELAMA KESEMBUHAN AUTO-SKIN GRAFT DENGAN WAKTU YANG BERBEDA) Erwin Erwin; Gunanti Gunanti; Ekowati Handharyani; Deni Noviana
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this research is to observe blood profile of domestic cats which includes red blood cell, white blood cell, haemoglobin, haematocrit, and trombocyte during skin graft recovery period via autograft. A total of nine male domestic cats (Felis catus) aged 1-2 years weighting 3-4 kg were separated into three treatment groups. Hair shaving and disinfectant application were done on lateral area of front leg, and then 2x2 cm incision was made. The wound was wrapped by sterile gauze dampened by poviderm iodine and left for different period of days per treatment group; where Group I (two days), Group II (four days), and Group III (six days). Transplantation was done by taking the skin on thorax area and placing it on the recipient after first cleaning subcutaneous tissue from the skin surface of donor’s wound and the base of recipient’s wound. Blood sample was taken from vena cephalica antebrachii anterior on day 0 before skin graft, on day three, six, nine, 12, and 18 after skin graft. Based on the result, significant difference waso bserved from red blood cell count, white blood cell count, haemoglobin count, and hematocrit, whereas trombocyte count did not show any significant difference between treatment groups. The cats on Group II and Group III were systemic good condition compared to Group I. ABSTRAK Penelitian ini bertujuan melihat profil darah kucing lokal, meliputi sel darah merah, sel darah putih, hemoglobin, hematokrit, dan trombosit selama kesembuhan tranplantasi kulit secara autograft. Sebanyak sembilan ekor kucing lokal (Felix catus) jantan umur 1-2 tahun dengan bobot badan 3-4 kg dibagi menjadi tiga kelompok perlakuan. Pada kucing tersebut dilakukan pencukuran rambut dan desinfeksi pada area kaki depan sisi lateral, selanjutnya dibuat luka sayat/incisi 2x2 cm. Luka dibalut dengan kasa steril yang dibasahi povidone iodine dan dibiarkan selama beberapa hari. Kucing dengan sayatan yang dibiarkan tersebut dikelompokkan menjadi kelompok I (dua hari), kelompok II (empat hari), dan kelompok III (enam hari). Transplantasi dilakukan dengan mengambil kulit dari area thorak dan ditempatkan pada resipien dengan terlebih dahulu permukaan luka kulit donor dan dasar luka resipien dibersihkan dari jaringan subkutis. Pengambilan sampel darah dilakukan melalui vena cephalica antebrachii anterior pada hari ke-0, 3, 6, 9, 12, dan 18. Berdasarkan hasil penelitian terjadi perbedaan yang signifikan (P<0,05) terhadap jumlah sel darah merah, sel darah putih, hemoglobin, dan hematokrit, sedangkan jumlah trombosit tidak menunjukkan perbedaan yang signifikan (P>0,05) di antara kelompok perlakuan. Kondisi sistemik tubuh kucing kelompok II dan III lebih baik dibandingan kelompok I.

The Kejadian Pertama Rabbit Haemorrhagic Disease Berdasarkan Studi Seroprevalensi di Provinsi Jawa Barat, Indonesia Retno Setyaningsih; I Wayan Teguh Wibawan; Surachmi Setiyaningsih; Ekowati Handharyani; Sri Murtini; Ahmad Biharidin
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.409

Rabbit Haemmorhagic Disease (RHD) is one of viral diseases in rabbits that is still exotic in Indonesia. The RHD disease is caused by the Rabbit Haemorrhagic Disease Virus (RHDV) which is a calicivirus of the genus Lagovirus, Caliciviridae family. The high genetic variation of the RHDV and the rapid spread have the potential to disrupt rabbit farm (rabbittery) production and trade activities, especially rabbit exports, which require clarity on the status and situation of RHD disease in rabbits in Indonesia. A case of suspected RHD in Indonesia was first detected in the Philippines where rabbits exported from Indonesia were detected to be seropositive to RHD which resulted in rabbit export activities to the country was stopped. There is no data on the presence of RHD disease in rabbits in Indonesia, so the seroprevalence study is useful to provide preliminary information on the presence of this disease in Indonesia. This study was conducted on 163 rabbits samples raised in the Lembang area, Bandung, West Java, which is known as the largest rabbit farming center in Indonesia. Rabbit samples were taken at rabbit farms spread across seven villages namely Lembang, Pagerwangi, Cikahuripan, Cikole, Sukajaya, Gudangkahuripan and Jambudipa. The rabbits serum samples obtained were tested using the Indirect Enzym Linked Immunosorbant Assay (ELISA) method to determine the titer of antibodies against RHD. Based on the results of the analysis, it is known that 120 out of 163 rabbit serum samples showed positive antibody titers against RHD. The presence of antibody titers in rabbits on such farms can be preliminary information to be able to carry out further studies.

Wound Healing Using White Turmeric (Curcuma zedoaria) Extract Nanoparticles: Macroscopic and Microscopic ObservationWound Healing Using White Turmeric (Curcuma zedoaria) Extract Nanoparticles: Macroscopic and Mi Fitri Ariyani; Lina Noviyanti Sutardi; Ekowati Handharyani
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.441

A wound is damaged of skin tissue caused by physical or mechanical trauma. Naturally, the wound healing process begins almost immediately after the injury occurred and is categorized by four main stage, these are hemostasis, inflammation, proliferation, and maturation. The aim of this research is to observe wound healing process using white turmeric (Curcuma zedoaria) nanoparticles extract gel based on macroscopic and microscopic observations. The skin of 24 Sprague dawley rats were cut 3 cm in the dorsal area and sutured with simple suture technique. The treatments were divided into four groups, positive control (C1), negative control (C2), gel nanoparticles white turmeric extract 0.75% (K1) and 1.5% (K2). Treatment was given every day for a week. Macroscopically observation were made descriptively on the 1st, 3rd, 5th, and 7th days. Histopatologically, the specimens were stained with hematoxylin eosin (HE) to observe the skin structure with parameters scab, epithelial formation, and neovascularization. The observations were evaluated on the 8th days post-injury. Data were presented descriptively through light microscope. The macroscopic results showed the nanoparticles extract gel treatment wound had a faster healing process and lower pain responses than the control groups. The microscopic showed the nanoparticles gel treatment extract had a better repairing process of skin structure and decreased inflammation cells than the control groups. In conclusion, the nanoparticles of white turmeric extract treatment gel group has a better results than the control groups.

Rumput Laut Sango-sango (Gracilaria sp.) Berpotensi Memperbaiki Kondisi Hiperglikemik dan Ekspresi Insulin Pankreas Tikus Diabetik Olga Purnama Bakti; Ekowati Handharyani; Sri Purwaningsih
Jurnal Veteriner Vol 24 No 2 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.2.179

Indonesia menempati urutan kelima dengan penderita diabetes melitus terbanyak di dunia. Manajemen makan harus dilakukan terutama pada penderita diabetes melitus yang menggunakan obat penurun glukosa darah atau insulin. Rumput laut sango-sango atau Gracilaria sp., memiliki potensi sebagai agen antidiabetes. Pengujian aktivitas antihiperglikemik pada model hewan diperlukan sebelum implementasi pada manusia. Penelitian dilakukan dengan pemberian pakan dengan penambahan Gracilaria sp. pada tikus dengan periode yang berbeda. Tikus diberi streptozotocin (50 mg/kg bb) untuk menginduksi diabetes. Hewan dibagi dalam enam kelompok perlakuan: kontrol, kontrol diabetes, tikus diabetes diberi pakan standar dan obat metformin, tikus diabetes diberi Gracilaria sp., selama tiga minggu dan dua minggu, dan tikus normal diberi Gracilaria sp., selama empat minggu. Parameter yang digunakan adalah glukosa darah dan luas area positif ekspresi insulin. Hasil yang didapatkan adalah pemberian streptozotocin mengakibatkan keadaan diabetes pada tikus. Pakan dengan penambahan Gracilaria sp., menunjukkan potensi untuk membantu manajemen hiperglikemik. Hasil yang signifikan ditemukan pada area positif ekspresi insulin. Pemberian pakan dengan penambahan Gracilaria sp., dengan hasil terbaik diperoleh pada pakan dengan pemberian selama tiga minggu.

Laporan Kasus: Studi Morfopatologi Demam Babi Afrika pada Babi di Bogor, Jawa Barat Rahayu Woro Wiranti; Dwi Utari Rahmiati; Aldo Yanuar Wuriyantara; Okti Nadia Poetri; Ekowati Handharyani
Jurnal Veteriner Vol 24 No 4 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.4.564

Demam Babi Afrika atau African swine fever (ASF) adalah penyakit menular yang dapat terjadi pada ternak babi dan babi liar. Penyakit tersebut disebabkan oleh virus ASF. Gejala klinis sangat bervariasi dari kasus yang terjadi perakut, akut, subakut dan kronis tergantung pada virulensi virus. Kejadian ASF dapat menyebabkan angka kematian tinggi pada babi, penurunan kondisi sosial, dan ekonomi termasuk kontaminasi lingkungan. Berdasar beberapa pertimbangan masalah tersebut, maka akan sangat bermakna bila dilakukan studi morfopatologi dan klarifikasi terhadap kejadian ASF pada babi (Sus scrofa domesticus). Kajian saat ini dilaksanakan dengan melakukan prosedur nekropsi terhadap dua ekor babi umur empat bulan, yaitu dengan melakukan pemeriksaan kondisi tubuh dan organ-organ interna. Sampel organ diambil untuk pemeriksaan histopatologi yang selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan lanjut dilakukan dengan metode polymerase chain reaction (PCR) menggunakan primer universal (p72 dan p54). Hasil pemeriksaan makroskopik menunjukkan bahwa beberapa bagian kulit mengalami erythema, terjadi splenitis hemoragi, enteritis hemoragi disertai dengan lymphadenopathy pada limfonodus mesenterika dan terjadi hemoragi pada meningen dan pembuluh darah di otak. Hasil pemeriksaan histopatologimenunjukkan lesi pada organ limforetikuler seperti limpa dan limfonodus mesenterika, yaitu radang limfositik disertai hemoragi. Pemeriksaan pada otak menunjukkan lesi radang limfositik disertai hemoragi pada selaput meningen. Hasil pemeriksaan molekuler dengan metode PCR memberikan konfirmasi bahwa penyakit disebabkan oleh virus ASF. Berdasarkan hasil pemeriksaan komprehensif secara makroskopik, mikroskopik dan evaluasi PCR dapat disimpulkan bahwa kedua ekor babi tersebut menderita infeksi ASF tipe akut.

Co-Authors ., Chairul A Winarto Adi Winanto Adi Winarto Afiff , Usamah Agus Buono Agus Setiyono Ahmad Biharidin Ahmad Sulaeman Akhmad Endang Zainal Hasan Aldo Yanuar Wuriyantara Armenia Eka Putriana Arni Diana Fitri Aulia Andi Mustika Aziz Kustiyo Bambang Kiranadi Bambang Sumiarto Bambang Widyo Prastowo BIBIANA W LAY Bohari Bohari Budiono, Novericko Ginger Chairul - Chairul Chairul Clara Meliyanti Kusharto Deni Noviana Denny Widaya Lukman Dewa, Wisnu Jaka DEWI APRI ASTUTI Dewi Ratih Agungpriyono Dewi Ulfa Trisdiani Dhany Nugraha Ramdhany Dondin Sajuthi Dwi Santy Damayati Dwi Utari Rahmiati Eflisa Endah Putri Elly Munadziroh Eny Palupi Erni Sulistiawati Erwin Erwin Etriwati E Evy Damayanthi Fadjar Satrija Fajar Kawitan Firmansyah, Hardi Fitri Ariyani Fitri, Arni Diana Franciscus Dhyana Giri Suyatna Geneva Ariesta Giriwono, Puspo E. Gunanti . Hadi Riyadi Handayu Untari Hapsari Mahatmi Hardi Firmansyah HERA MAHESHWARI Hernomoadi . Hernomoadi Hernomoadi Herwin Pisestyani Hidayah Dwiyanti Huda Shalahudin Darusman I wayan Teguh Wibawan Ikeu Ekayanti Irma Herawati Suparto K Ochiai Karsi Ambarwati Katrin Roosita KHOIRIYAH, ROMYUN ALVY Kiranadi Bambang Komariah Komariah Komariah Lili Andriani Lina Noviyanti Sutardi M.B. Malole . MARIA BINTANG Masdiana C. Padaga Masriani . Nabilah, Avida Shahnaz Nasution, Zuraidah Ni Luh Putu Ika Mayasari Nisrina Rosyida Noor Rifai Novera Nirmalasanti Nugraha, Arifin Budiman Nugroho, Setyo Widi Octarina Okti Nadia Poetri Olga Purnama Bakti Opyn Mananta Perdhana, Ika Satya Putra Wijaya, Erlangga Putri Puncak Anjani R. Susanti Rahayu WoroWiranti Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Setyaningsih Rimbawan , Rimbawan Rimbawan Risti Rosmiati Riyadh Firdaus Rizal M Damanik, Rizal M Ruqiah Ganda Putri Panjaitan safrina dyah hardiningtyas Sangko Sayuti Nasution Sangko Sayuti Nasution, Sangko SEDARNAWATI YASNI SILMI MARIYA Sophia Setyawati Sri Anna Marliyati sri murtini . Sri Purwaningsih Sri Rahmatul Laila Sri Wahyuni Suhendi, Adnan Rizal Surachmi Setiyaningsih Tanjung Penataseputro Tantri, Aida Rosita Trini Suryowati, Trini Umi Cahyaningsih Wasmen Manalu Wibowo, Artdhea Regita Wiwin Winarsih Yetty Ramli Yuli Purwandari Kristiangingrum Yuni Andriani Yusuf Ridwan Zulfa Zakiah

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