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Biology Education Program, Faculty Of Teacher Training And Education, Universitas Khairun, Indonesia
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Journal : Jurnal Biosilampari: Jurnal Biologi

 1 
IDENTIFIKASI JENIS LEBAH MADU ASAL KEPULAUAN SULA MENGGUNAKAN APLIKASI DNA BARCODE LCO GEN Mas'ud, Abdu; Hasan, Said; Sundari, S.
Jurnal Biosilampari : Jurnal Biologi Vol 5 No 2 (2023): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v5i2.1888

Abstract

Sanana Honey is a local brand, a type of honey produced by forest bees which is well known in the Sula Islands, North Maluku. The forest bees of the Sula Islands have the potential to be developed as a producer of quality honey. At this time there has been no scientific report regarding the types and characteristics of the forest bee species from the Sula Islands. This study aims to identify the types of honey-producing forest bees by applying the LCO gene DNA barcode. The methods used in this study used: 1) DNA extraction from bee body tissues using the DNA Miniprep Kit procedure (Zymo Research, D6016); 2). DNA amplification by PCR amplification procedure with TOYOBO KOD FX Neo (KFX-201); 3) bidirectional DNA sequencing process (Bi-directional sequencing). The results showed that the specimen was successfully amplified with an amplicon size of 677 bp. Furthermore, the results of BLASTN analysis revealed that the sequence has a 97.78% similarity with the Apis cerana mitochondria complete genome gene country of Russia, but is phylogenetically different between the two.
Co-Authors A. R. Tolangara, A. R. A.D. Corebima Abdu Mas'ud Abdu Mas’ud Abdul Rahman Jannang Abdullatif, Zauzah Abu Mas'ud Abubakar Abdullah Abubakar, Salim Ade Haerullah Agus Hanif Ahmad, Hasna Ahmad, Iswdi M. Akmal, Nurul Akmal, Nurul Alisi Alisi Amalia, Berlyana Amina Puradin Angga Ardiansyah Annisa, Nidya Arifianti, Aprillia Arini Z. N. Aryuni, Vrita Tri Asrul Dedy Ali Hasan Azmi, Difa’ Bahtiar Bahtiar Basalamah, Muhammad Syafii A. Basyir, Rahma N Buyung La Payama Chumidach Roini Danissa, Fanny Dian Nugraheni Ermin ermin Erti Hamimi Fadillah Adnan Fahria T. Kasuba Fardhani, Indra Firjan Hi Sabtu Habiddin Haji, Sulfi Abd Harianto Hamidu Harwani Harwani Hasan, Asrul Dedy Ali Hasanudin, Mahyono Ibrahim Ibrahim Ichsan, Mochammad Ilham Majid Indah Juwita Sari Iqbal Limatahu Iswadi M Ahmad Jailan Sahil Jamaludin Sehat Jassia I. Pagala K Kartika Kasuba, Fahria T. La Ode Muhammad Azhar Laekkeng, Mursalim Limatahu, Iqbal M Nasir Tamalene M Nur Kadir Mahyono Hasanudin Mardia Hi Rahman, Mardia Hi Mariyani Hi. Tamrin Marlina Hamisi Marwan Man Soleman Marwan Marwan Marwan Marwan Marwan Marwan Marwan, Marwan, Marwan,, Marwan, Mas'ud, Abdu Masud, Abdu Milsan Saban Mochammad Hannats Hanafi Ichsan Muhammad Amrullah Pagala Muhammad Fajar Marsuki Muhiddin muhiddin Muhktar Yusuf N., Arini Z. Nuraini Sirajudin Nurhasanah, Nurhasanah Nurmaya Papuangan, Nurmaya Pagala, Jassia I. Putri, Novi Eka Regina Regina Rugaya H. Serosero S. Sundari Saban, Milsan Sahnaz Hi. Ahmad Sahnaz Hi. Ahmad Saibi, Ningsi Sakdiah Ibrahim, Sakdiah Ibrahim Serosero, Rugaya H. Sindi Kharomah Siti Khadijah Sjamsudin, Salma Sugiyanto, Sugiyanto Sundari Sundari Sundari Sundari Suparman Suparman Suparman Suparman Suparman Suparman Suryati Tjokrodiningrat Taher, Dharmawaty M. Tiara Pramesti C. Ibrahim Triyono Triyono Ula, Nurin Ni’matul Ummiyati Ummiyati Wicaksono, Azizul Winarmi, Nasrunniati Dyahayu Anggi Yayuk Mulyati Yumima Sinyo Yumima Sinyo Yundari, Yundari Yunus Syafie Yunus Syafie Yusmar Yusuf Yusnaini Yusnaini Yusnaini Yusnaini Yusri Sapsuha Zauzah Abdullatif Zulkifli Ahmad ‘Ainunnizar, Desy