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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Journal of Marine and Aquatic Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science LAW REFORM Jurnal Hortikultura Prosiding Seminar Biologi Biota BIOEDUSCIENCE Jurnal Perikanan Universitas Gadjah Mada Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Scripta Biologica Musamus Fisheries and Marine Journal International Journal of Environmental, Sustainability, and Social Science Makara Journal of Science Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) BIO-CONS: Jurnal Biologi dan Konservasi BIOSAPPHIRE: Jurnal Biologi dan Diversitas SIMBIOSIS Metamorfosa: Journal of Biological Science Open Access DRIVERset
IDA AYU ASTARINI
Biology Study Program, Faculty Of Mathematics And Natural Sciences, Udayana University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Genetic Diversity and Phylogenetic of Longtail Tuna (Thunnus tonggol) Landed in Pabean Fish Market, Surabaya Ida Ayu Astarini; Shella Ayu Ardiana; I Nyoman Giri Putra; Putu Dian Pertiwi; Andrianus Sembiring; Astria Yusmalinda; Danie Al Malik
Musamus Fisheries and Marine Journal Volume 3 Number 2, April 2021
Publisher : Faculty of Agriculture Musamus University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/mfmj.v3i2.3375

Abstract

Indonesia is the biggest tuna exporter in Southeast Asia. With a high number of tuna catch, it is worried that the catch will decrease tuna population, specifically longtail tuna. To anticipate the decrease, there needs to be a conservation program to protect longtail tunas from scarcity. One method used to protect longtail tuna is by genetic conservation. The aim of this research is to understand the genetic and phylogenetic variety of the longtail tuna in Pabean Surabaya Fish Market. The polymerase chain reaction was used to amplify segment of the mitochondrial control region gene from members of these sample, used forward primer CRK 5’-AGCTC AGCGC CAGAG CGCCG GTCTT GTAAA-3’ and reverse primer CRE 5’-CCTGA AGTAG GAACC AGATG-3’. Based on the sequencing process, 28 out of 29 samples longtail tuna were analyzed successfully. The results of the 28 sample analysis of longtail tuna based on its genetic variety and phylogenetic tree reconstruction showed a haplotype variety (Hd) score of 1,00000, and nucleotide (π) variety of 0,1939. Genetic variety value showed that longtail tuna has great adaption capabilities toward environmental changes time to time. Phylogenetic tree reconstruction results showed 7 clades with a genetic range of 0,005 – 0,035, which shows that all samples are closely related. The results of this study can be used as basic information in forming regulations on longtail tuna sustainable management and genetic conservation.
EFEKTIVITAS MIKROALGA Chlorella vulgaris DAN Spirulina plantensis DALAM BIOSORPSI LOGAM NIKEL DI PERAIRAN (KASUS PERAIRAN POMALAA KABUPATEN KOLAKA) Ahmad Zakir; I Wayan Budiarsa Suyasa; Ida Ayu Astarini
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 16 No 1 (2022)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/EJES.2022.v16.i01.p08

Abstract

Mining and nickel processing activities in Pomalaa, Kolaka Regency have an impact on the condition of coastal waters. Several researchers who have conducted research in the area stated that the Pomalaa coast has been polluted by the presence of nickel metal so that there is an initiative to reduce the impact of Ni in the waters by bioremediation of the microalgae Chlorella vulgaris and Spirulina platensis. This study aims to analyze the growth of microalgae types C. vulgaris and S. platensis cultured on media containing nickel with different concentrations and also to determine the ability of microalgae to reduce nickel heavy metals at different concentrations. This research was carried out at the Fisheries Laboratory of Haluoleo University , while the AAS (Atomic Absorption Spectrophotometric) analysis was carried out at the Kendari Environmental Laboratory UPTD Laboratory on January – May 2021. The results of the analysis showed that there was a significant difference in the growth density of microalgae cells (C. vulgaris and S. platensis) which were given the treatment/concentration. Ni different. The absorption capacity of C. vulgaris ranged from 0.73 to 4.97 mg/l, while s. platensis ranged from 0.082 – 2.11 mg/l. C. vulgaris is superior in reducing nickel because C. vulgaris can live in polluted water conditions. Keywords: Bioremediation; Nickel; C. vulgaris; S. platensis.
Callus Induction In Leucaena (Leucaena leucocephala (Lam.) de Wit) As An Effort To Provide Target Transformation Through Agrobacterium tumefaciens Ni Nyoman Nila Arieswari; Ida Ayu Astarini; Junita Hardini; Austin Ryan Garrido; Debora Margareth; Jennifer Crismonika; Sebastian S. Cocioba
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p02

Abstract

Leucaena is a plant that produces biomass productivity in the form of hardwood for fuel with low humidity and high calorific value. However, Leucaena is also classified as an invasive plant which can cause the urgency of native plant species and ecosystems in Indonesia. Therefore, the formation of sterile Leucaena needs to be done, one of which is through genetic transformation using Agrobacterium tumefaciens. Callus is used as a target for transformants in the genetic transformation process, so it is necessary to use appropriate media and PGR. This study aimed to determine the type of media and the concentration of 2,4-D on callus induction. This research is an experimental study with a completely randomized design (CRD) method with two factors. The first factor is the type of media (MS and WPM) and the second factor is the concentration of 2,4-D (0; 0.25; 0.50; 0.75; 1.00; 1.25 and 1.50 mgL-1 ). Each treatment was repeated three times so that 42 experimental units were obtained. Parameters observed were callus initiation, callus fresh weight (gram), callus texture and color. Quantitative data is analyzed by analysis of variance (ANOVA). The results showed that the use of media had a significant effect (P<0.05) on callus fresh weight. The use of 2,4- D concentration had a significant effect (P>0.05) on callus texture. The use of WPM media resulted in the fastest callus emergence time (6.67±0.57), the best callus texture (crumb callus type 2) and the best callus color (green). Meanwhile, the highest fresh weight (2,48±0.83) was in the use of MS media. The fastest callus emergence time occurred in the control (without the addition of 2,4-D) (7.33±0.57 and 6.67±0.57), the highest average fresh callus weight (2,48±0.83 and 2.35±0.32) occurred in the treatment with the addition of 1.00 mgL-1 2,4-D with a crumb callus texture of type 2 and callus green color only appeared in the treatment with a concentration of 0.25 mgL-1.
The Effect Combination of Glomus Endomycorrhizal and Trichoderma on The Productivity of Corn (Zea Mays L.) Maghfirotun Nisa; Meitini Wahyuni Proborini; Ida Ayu Astarini
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p04

Abstract

The aim of the research was to determine the effect of the combination of Glomus endomycorrhizal and Trichoderma and the appropriate dose to increase the productivity of maize (Zea mays L.). The study was began with the propagation of Glomus endomycorrhizal spores in the green house of the Biology Study Program, Faculty of Mathematics and Natural Sciences, Udayana University and continued with endomycorrhizal spore screening, re-isolation of Trichoderma fungi and application of Glomus and Trichoderma endomycorrhizal spores to corn plants. The results showed that there was no significant difference between positive control (KNO3) and endomycorrhizal and Trichoderma treatments on plant height, number of leaves, root length, ear length, ear diameter, ear wet and dry weight and shell weight and significantly different on the small size of the shells. Doses of 125 Glomus spores and 5 mL Trichoderma give the best results on the small amount of corn shells compared to other doses, so that the combination of endomycorrhizae and Trichoderma can be applied to reduce excessive chemical fertilizers.. Keyword: biofertilizer, growth, nutrient, productivity
Analyze Of Cloves (Syzygium aromaticum L.) Leaves Essential Oil Grow From Different Elevations Range Luh Gede Artha Saridewi Wijaya; Ni Luh Arpiwi; Ida Ayu Astarini
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p15

Abstract

ABSTRACT A research was carried out to determine the yield, content of constituent compounds, and analysis of the quality of clove leaf essential oil (Syzygium aromaticum L.) at different altitudes. The study used dry clove leaves obtained from Buleleng Regency, Bali Province with an altitude of 300 meters above sea level (masl), 600 masl, and 900 masl. Extraction of essential oils was carried out by steam distillation. The essential oil obtained was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and the quality was tested using the quality requirements of SNI 06-2387-2006. Parameters observed were yield, identification of constituent compounds, analysis of essential oil quality including color, odor, specific gravity, and refractive index, solubility in alcohol, optical rotation, total eugenol and caryophyllene. The results showed that clove leaf essential oil in the lowlands (300 masl) had a higher yield of 2.05% and more types of active compounds than in the optimal altitude (600 masl) which was 1.45% and the highlands (900 masl) was 1.85%. The main compounds that make up clove leaf essential oil were eugenol, caryophyllene, and humulene. Other compounds were longifoline, nootkatone, naphthalene, citronellal, and cyclohexene. The clove leaf essential oil from the three heights met the quality requirements of SNI 06-2387-2006. Keywords: Essential oil, cloves, eugenol, GC-MS, secondary metabolites
Keragaman Genetik Ikan Tongkol Abu-Abu (Thunnus tonggol) yang Didaratkan di Pasar Ikan Sagulung, Batam, Kepulauan Riau Berdasarkan DNA Mitokondria Suryany Devy; Ida Ayu Astarini; I Nyoman Giri Putra; Andrianus Sembiring; Luh Astria Yusmalinda; M. Danie Al Malik; Ni Putu Dian Pertiwi
Journal of Marine and Aquatic Sciences Vol 7 No 2 (2021)
Publisher : Fakultas Kelautan dan Perikanan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/jmas.2021.v07.i02.p06

Abstract

Longtail Tuna (Thunnus tonggol) is one of the neritic species of tuna from the Scrombridae family. T. tonggol is oceanicromus and ussually found in tropical and subtropical waters in the Indo-Pacific region. Although known as one of the tuna species, the information of this species is very lacking, especially in Indonesia. Therefore, this study aims to determine the genetic diversity and kinship relationships of longtail tuna (T. tonggol) collected at Sagulung fish market, Batam, Riau Islands. Molecular analysis were carried out using genetic markers namely mitochondrial D-loop (control regian) with the stages of DNA extraction, PCR (Polymerase Chain Reaction), electrophoresis, sequencing, and data analysis. A total of 21 longtail tuna samples were identified using molecularly and confirmed as T. tonggol species, which have a base length ranging from 482 - 523 bp (base pairs). The value of haplotype diversity (Hd) of longtail tuna was 1,00000 and the value of nucleotide diversity (?) was 0,01654. The results of phylogenetic tree reconstruction showed that all samples collected were in the same group (clade) with the average genetic distance in one T. tonggol clade obtained a value of 0.010 which has a close relationship between samples. This result can be use as additional information for longtail tuna species in Indonesia.
STRATEGI PENGELOLAAN EFEKTIF EKOSISTEM TERUMBU KARANG DI KAWASAN KONSERVASI NUSA PENIDA I Dewa Kadek Wira Sanjaya; I Nyoman Merit; Ida Ayu Astarini
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 16 No 2 (2022)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/EJES.2022.v16.i02.p01

Abstract

Nusa Penida MPA was established with the aim to conserving natural resources and ensuring the sustainability of livelihoods. The Government of Indonesia uses the EVIKA tools to assess the effectiveness of MPA management. This study examines the effectiveness of MPA management by looking at indicators of coral reef ecosystem health and resource utilization to develop an effective management strategy using the force filed analysis method. The results showed that the condition of coral and fish biomass in the Nusa Penida MPA experienced a significant increase. Hard coral cover was in the medium category with a cover of 43% and fish biomass of 816.80 kg/ha. The coral reef health index of the Nusa Penida MPA is 5 which indicates that coral cover is in the medium category with high recovery potential and the coral reef fish category is low. Nusa Penida marine resources are used for fisheries, tourism as well as for trade. This study recommends a strategy to increase the effectiveness of Nusa Penida MPA management by investing in increasing human resource capacity in the Bali MPA management unit in competence on monitoring ecosystem and preparing a management budget plan along with efforts to fulfill program budgets. Keywords: Coral Reef, Management; Effective; Nusa Penida; MPA
PERBANYAKAN DAN PEMANFAATAN TANAMAN HIAS OBAT DAN UPACARA UNTUK PELUANG WIRAUSAHA MASYARAKAT DESA JUNGUTAN KARANGASEM BALI M.R. Defiani; I.A. Astarini; I.M.S. Wijaya; E. Kriswiyanti; A.A.S.A. Sukmaningsih K; I.B.W. Gunam
Buletin Udayana Mengabdi Vol 22 No 2 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i02.p10

Abstract

Community service for PKK members and youth organizations at the Samsara Living Museum, Jungutan Village, Karangasem, Bali aimed to assist preserve ceremonial plants which include ornamental plants and coconuts of various types and the introduction of blue tea from Clitoria ternatea and turmeric drink for the use of medicinal plants. The initial method was carried out with a site survey to take an inventory of plants at the service location, then socialization with participants was carried out through poster presentations and then continued with the practice of plant nurseries and making blue tea and taste testing of herbal turmeric and tamarind drink. Based on the results of the implementation in the field, most of the participants were new to the existence of blue tea which is beneficial for health because it contains antioxidants. The vegetative propagation of ornamental plants with high economic value such as aglonema, Sanseviera and red betel was very interesting for the participants to increase the effort of propagation of ornamental plants that could be done easily. The introduction of some coconuts such as Mulung coconut which can be used to maintain the body's immunity during a pandemic. The turmeric and tamarind drink helps stamina during the pandemic and can also be tried by participants and has been used as a 'welcome drink' for visitors to Samsara. Some PKK members have tried to grow eggplant and chili vegetables in their yard and are interested in increasing ornamental plants for entrepreneurial activities to motivate other PKK members. Keywords: cutting, grafting, seed, tuber, community empowerment
PENCEGAHAN BROWNING PADA EKSPLAN IN VITRO UNTUK PERBANYAKAN TANAMAN Lilium longiflorum Ni Kadek Dwipayani Lestari; Ni Wayan Deswiniyanti; Ida Ayu Astarini; Luh Made Arpiwi
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol 1 (2018): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (848.917 KB)

Abstract

ABSTRAKTanaman Lilium longiflorum pada umumnya diperbanyak dengan menggunakan pemisahan umbi, namun terdapat kendala yaitu tanaman yang diperbanyak terbatas, memerlukan lahan dan tergantung kondisi cuaca dengan waktu yang relatif cukup lama. Maka, diperlukan metode baru yaitu salah satunya metode kultur in vitro karena metode ini tidak tergantung pada lahan, cuaca dan dapat memperbanyak tanaman dengan jumlah yang banyak dan cepat ,namun metode ini pun memiliki kelemahan yaitu adanya kontaminasi serta terjadi browning pada eksplan, maka diperlukan suatu metode untuk mengatasinya. Penelitian ini bertujuan mengetahui metode eliminasi browning yang efektif diantara perlakuan perendaman eksplan dalam asam askorbat dan asam sitrat. Pengamatan meliputi waktu rata-rata eksplan mengalami browning, intensitas browning seluruhnya atau sebagian dan persentase eksplan browning hingga 35 hari setelah tanam (HST). Berdasarkan hasil yang diperoleh dari perlakuan perendaman asam sitrat 500 mg/l dan asam askorbat 300 mg/l selama 30 menit dapat menurunkan waktu inisiasi browning hingga 88 % pada eksplan daun muda, 100 % pada eksplan pedicel dan 64 % pada eksplan ovarium. Menurunkan intesitas browning pada eksplan daun muda dan ovarium. Menurunkan persentase browning sebesar 80% pada eksplan daun, 0% pada eksplan pedicel dan 36% pada eksplan ovarium.Kata kunci : browning, eksplan, asam sitrat, asam askorbat, liliumABSTRACTLilium longiflorum plants are generally propagated by using bulbs, but there are some so-called limited-multiplied plants, broad conditions and depending on the conditions for a long time. So, a new method is needed, which is one of the culture methods in vitro because this method does not depend on the land, weather and can multiply plants in large and fast numbers, but this method also has weaknesses, such as mathematics and browning occurs in explants, so some things required. methods to overcome them. This study aims to eliminate the effect of effective skin color between soaking treatment in ascorbic acid and citric acid. Observations include the average time of brown, brown, or part and all explants explants to reach 35 days after planting (HST). The results obtained from soaking citric acid 500 mg / l and ascorbic acid 300 mg / l for 30 minutes can reduce browning initiation time by 88% on young leaf explants, 100% on pedicel explants and 64% on ovarian explants. Reduces browning intensity on young leaf and ovarian explants. Reducing the amount of browning by 80% on leaf explants, 0% on pedicel explants and 36% on ovarian explants.Keywords: browning, explants, citric acid, ascorbic acid, lilium
EKSTRAKSI DNA DAUN DAN BIJI JARAK PAGAR (Jatropha curcas L.) DENGAN METODE DOYLE DAN DOYLE (1990) Ni Wayan Deswiniyanti; Ida Ayu Astarini
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol 1 (2018): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (955.477 KB)

Abstract

ABSTRAKJarak pagar (Jatropha curcas L.) merupakan salah satu tanaman sebagai sumber biodiesel yang memiliki beragam aksesi. Cara untuk membedakan masing – masing aksesi dapat dilihat berdasarkan dari ciri morfologinya atau berdasarkan marka DNA. DNA dapat diekstraksi dari seluruh bagian tanaman. Pada penelitian ini DNA diekstrak dari sampel daun dan biji jarak pagar dengan menggunakan metode Doyle dan Doyle (1990). Penelitian ini bertujuan untuk mengetahui metode dan hasil ekstraksi DNA dari berbagai jenis sampel dari bagian tanaman jarak pagar antara lain biji (J1), daun segar (J2), daun yang telah disimpan dalam lemari es suhu 40 C (J3), daun muda (J4) dan daun tua (J5) yang telah dibekukan pada suhu -800 C selama 1 minggu. Ekstraksi DNA menggunakan buffer ekstraksi dengan komposisi 2% CTAB (Cetyltrimethyl Ammonium Bromide), 100 mM Tris/HCl, pH 8, 1.4 M NaCl, 20 mM EDTA, pH 8.0, 1% polyvinylpyrrolidone (PVP)-40, 2% mercaptoethanol dan elektroforesis menggunakan gel agarosa 0,8% dalam larutan TAE 0,5X. Hasil ekstraksi pada semua sampel J1, J2, J3, J4 dan J5 dapat menunjukkan pita DNA sehingga sampel untuk ekstraksi DNA dapat menggunakan salah satu jenis bahan ekstrak dari berbagai sampel biji dan daun dengan atau tanpa perlakuan penyimpanan untuk penelitian marka DNA aksesi tanaman jarak pagar.Kata kunci: Ekstraksi, DNA, Doyle dan Doyle (1990), Jarak pagar, Biodiesel.ABSTRACTJarak Pagar (Jatropha curcas L.) is one of the plants as a source of biodiesel that has various accessions. The ways to distinguish each accession can be seen based on morphological characteristics or based on DNA markers. DNA can be extracted from all parts of the plant. In this study DNA was extracted from Jatropha curcas L. leaves and seeds samples using the Doyle and Doyle (1990) method. The aims of this study is to determine the methods and results of DNA extraction from various types of samples from J. curcas L. plant parts including seeds (J1), fresh leaves (J2), leaves that have been stored in a refrigerator at a temperature of 40 C (J3), young leaves (J4 ) and old leaves (J5) which have been frozen at -800 C for 1 week. DNA extraction using an extraction buffer with a composition of 2% CTAB (Cetyltrimethyl Ammonium Bromide), 100 mM Tris / HCl, pH 8, 1.4 M NaCl, 20 mM EDTA, pH 8.0, 1% polyvinylpyrrolidone (PVP) -40, 2% mercaptoethanol and electrophoresis using 0.8% agarose gel in 0.5X TAE solution. Extraction results on all samples J1, J2, J3, J4 and J5 can show DNA bands so that samples for DNA extraction can use one type of extract material from various seed and leaf samples with or without storage treatment for research on Jatropha plant accession DNA markers.Keywords: Extraction, DNA, Doyle and Doyle (1990), Jatropha curcas L., Biodiesel.
Co-Authors A A Ayu Laksmi Damarnegari A.A.S. A. Sukmaningsih Ahmad Zakir Anak Agung Gde Raka Dalem, Anak Agung Gde Raka Andrianus Sembiring Andrianus Sembiring Angel Chappell Anitasari, Septarini Dian Astria Yusmalinda Austin Ryan Garrido Catarina Tenny Setiastri Cornelia Coraima Lazaren Dalem, A.A. Gde Raka Danie Al Malik Debora Margareth Debora Margareth Dian Catur Prayantini Douglas Scheuring Dwi Nur Rikhma Sari Edelmiro Jose De Deus Elok Faiqoh Ema Hendriyani Enex Yuniarti Ningsih Eniek Kristiyanti ENIEK KRISWIYANTI GUIJUN YAN Gusti Ayu Nyoman Budiwati Hermanto, Bagus I Dewa Gede Warmadewa I Dewa Kadek Wira Sanjaya I G. R. M. TEMAJA I Gede Mahardika I Gusti Ayu Putri Kartika, I Gusti Ayu I Gusti Ayu Sugi Wahyuni I Gusti Bagus Sila Dharma I Gusti Bagus Sila Dharma I Gusti Made Oka Nurjaya I GUSTI NGURAH SANTOSA I Ketut Catur Marbawa I Ketut Ginantra I Made Antara I Made Saka Wijaya I Nyoman Gede Wisesa Adnyana I NYOMAN MERIT I NYOMAN RAI I W BUDIARSA SUYASA I Wayan Arthana I Wayan Gede Astawa Karang I Wayan Krisna Eka Putra I Wayan Krisna Eka Putra I Wayan Suarna I WAYAN WINDIA I.G.M Oka Nurjaya I.M.S. Wijaya Ida Ayu Putri Darmawati Ida Bagus Wayan Gunam Indah Tria Hoky Jennifer Crismonika Jeremy Pramana Josina Irene Brigita Hutubessy JULIAN CREIGHTON MILLER JR Julian Creighton Miller Jr. JULIE ANNE PLUMMER Junita Hardini Kadek Andina Widiastuti Kadek Andina Widiastuti Kriviņš, Anatolijs Luh Astria Yusmalinda Luh Gede Artha Saridewi Wijaya Luh Made Arpiwi LUH Puru ESWARYANTI KUSUMA YUNI M. Danie Al Malik MADE PHARMAWATI Made Ria Defiani Made Wahyu Cerianingsih Maghfirotun Nisa Meitini Proborini Wahyuni Mia Kosmiatin Miller, Jr, J. Creighton Mohammad Subhan Muhammad Danie Al Malik Muhammad Ibadullah Nathalia Sihombing Ni Kade Ayu Purnama Adi Ni Kadek Dwipayani Lestari Ni Kadek Raleni Ni Kadek Sari Dewi Handayani NI KADEK YUNITA SARI Ni Ketut Mas Suratniasih Ni LUH ARPIWI Ni Luh Astria Yusmalinda Ni Luh Putu Kayika Febryanti Ni Luh Suriani Ni Made Ary Widiastiti NI MADE DIAN PRATIWI Ni Made Puspawati NI NYOMAN ARI MAYADEWI Ni Nyoman Ely Kristiyanti Ni Nyoman Nila Arieswari Ni Nyoman Nila Arieswari Ni Putu Adriani Astiti Ni Putu Adriani Astiti Ni Putu Anom Sulistiawati, Ni Putu Anom Ni Putu Dian Pertiwi Ni Putu Vivin Nopiantari Ni Putu Yuni Astriani Dewi Ni Wayan Deswiniyanti Onasys , Yonatan Putra, I Nyoman Giri Putu Dian Pertiwi Putu Dian Pertiwi Putu Gede Arya Sumertayasa Putu Kayika Febryanti, Ni Luh Putu Wina Andriani Lestari Rindang Dwiyani SANG KETUT SUDIRGA Sean Michael Thompson Sebastian S. Cocioba Septarini Dian Anitasari Septarini Dian Anitasari Septarini Dian Anitasari Shella Ayu Ardiana Suryany Devy Taebenu, Desi Adriyanti Nina Tarigan, Swary Dwi Suherna Tiwi Wati VEMY CLAUDIA Wildan Mujahidul Basyar Yuli Setiawati YUNALDI YUNALDI YUNITA HARDINI Zaenul Gafari