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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Gadjah Mada International Journal of Business Journal of Tropical Biodiversity and Biotechnology Science and Technology Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Riset Akuakultur JSFK (Jurnal Sains Farmasi & Klinis) Jambura Journal of Chemistry Majalah Farmasetika JURNAL KAJIAN VETERINER Narra J Makara Journal of Science Gunung Djati Conference Series Jurnal Kedokteran Hewan Current Biomedicine Policy Brief Pertanian, Kelautan, dan Biosains Tropika Borneo Journal of Pharmacy Berita Biologi
Huda Shalahudin Darusman
IPB University
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Makara Journal of Science

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Development and Optimization of an Immunoassay for the Detection of Antibodies Against SARS-CoV-2 with In-house Recombinant RBD Protein Ratu, Safira Pinaka Pramestika; Mariya, Silmi; Noviana, Rachmitasari; Saepuloh, Uus; Darusman, Huda Salahudin
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

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Abstract

COVID-19 caused by SARS-CoV-2 poses a major threat to the global community, particularly in Indonesia. Countermeasures to prevent the spread of this disease have also been implemented, including the implementation of a vaccination program. An immunoassay technique that can be used to analyze antibodies that might develop following vaccination is the indirect enzyme-linked immunosorbent assay (ELISA). We produced the recombinant spike protein used in this study. The optimization comprised adjusted concentrations of spike recombinant protein (5 and 10 ng/mL), blocking agent (2.5% and 5%), and conjugate (1:1000 and 1:5000). The optimal conditions in this study included a spiked concentration of 10 ng/mL, a blocking agent concentration of 5%, sample dilution of 1:33, and a conjugate concentration of 1:1000. The intra-assay value of this optimized indirect ELISA was 7.3, and the inter-assay value was 5.3. The commercial MyBioSource kit and immunodiagnostic were utilized as a reference in the T-test, with P-values of 0 and 0.313, indicating that the recombinant protein in-house ELISA kit in this study demonstrated the same ability as the commercial immunodiagnostic kit in detecting SARS-CoV-2 antibodies, allowing it to be used for post-vaccination efficacy evaluation.
Co-Authors A.S. Satyaningtijas Achmad, Yusuf Farid Adi Winarto Adi Winarto Agik Suprayogi Ahmad Kamal Nasution Ahmad Subhan Ai Hertati, Ai Akhiruddin Maddu Andhika Yudha Prawira Andi Hiroyuki Angela Mariana Lusiastuti Anita Esfandiari Annisa Dewi, Fitriya Nur Apon Zaenal Mustopa Aprilyana, Lia Ardiansyah Nurdin Benvika Chairunnisa, Sheila Damiana Rita Ekastuti Dayana, Hepy Dewi Ratih Agungpriyono Dewi, Fitriya Nur Annisa Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Ekawati, Nurlaili Ekowati Handharyani Elok Budi Retnani Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Ernes Andhesfa Etih Sudarnika Fadla, Ilma Nadya Fauzi Abdurrahman Munggaran Femke Den Haas Ferdinandez, Mariana Firdaus, Moh Egy Rahman Fitri, Arni Diana Habib Ilham Maulana Al Adhim Hana Intishar Sawitri Hardo Basuki Harrold Halilintar MR Subu Taopan Hasya, Karin katina HERA MAHESHWARI Hidayaturrahmah Hidayaturrahmah I wayan Teguh Wibawan Ice Lusia Marta IETJE WIENTARSIH Ikramullah, Muh. Chaeril Indra Bachtiar Indra Bachtiar Indriawati, Iin Iqbal Ngabdusani Irawan, Herman Irawan, Shasmita Irma Herawati Suparto Jendri Mamangkey Joko Pamungkas Jusak Nugraha Kanti Rahmi Fauziyah Katrin Roosita koekoeh santoso Krisnayanti, Ni Putu Eka Lina Elfita Lina Elfita Linda Tjhin Lis Rosmanah Loe, Fhady Risckhy Loes (ML) Schure Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Megawati Iskandar Mia Audina Muhammad Hamdan Mustopa, Apon Z. Ningrum, Emilna Mega Novi Mayasari Noviana, Rachmitasari Novianti, Ela Novriyandi Hanif Nugroho, Setyo Widi Nurfatwa, Maritsa Perdhana, Ika Satya Permanawati Permanawati PERMANAWATI, PERMANAWATI Puji Rianti Putri, Indah Aprianti Rachmitasari Noviana Rahmania Hanim Rahminiwati, Min Randall C. Kyes Rasyida Ulfa Ratna, Lita Tri Ratni Ernita Ratu, Safira Pinaka Pramestika Raymond R. Tjandrawinata Ridzki M.F. Binol Rifqiyah Nur Umami, Rifqiyah Nur Rika Sudranto Rizal, Muhammad Farid Rizki Aulia Ansari Roostita L Balia Rosmanah, Lis Rosyid, Bahaudien Sari, Isti Kartika Savitri Novelina Sela Septima Mariya Setyawaty, Dyah Setyo Widi Nugroho Sibit, Denniswara Silmi Mariya Silmi Mariya SILMI MARIYA Silvy Thiyana Soedarso, Yovi Alwi Sony Hartono Wijaya Sri Wahyuni Srihadi Agungpriyono Subu Taopan, Harrold Halilintar MR Suduri, Adam Sukenda Sukenda Sumitro - Sundari, Betty Suparto, Irma H. SURYA, MARYATI Suryo Saputro Tambunan, Anggitha Tatang Mitra Setia Tatik Mufidah Tommy Langgeng Abimanyu Tri Wibawa Uus Saepuloh UUS SAEPULOH Uus Saepuloh Vinka Aftinata Kusumaputri Wartika Rosa Farida Wasmen Manalu WIDANARNI WIDANARNI Wira, Dwi Wahyuda Yuliana Yuliana Yuliana Zaelani, Bella Fatima Dora