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All Journal UNEJ e-Proceeding JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Jurnal Dinamika Pengabdian (JDP) Universa Medicina Journal of Islamic Medicine CARADDE: Jurnal Pengabdian Kepada Masyarakat PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JFIOnline Community Development Journal Indian Journal of Forensic Medicine & Toxicology Humanism : Jurnal Pengabdian Masyarakat Berkala Ilmiah Kimia Farmasi Berkala Penelitian Hayati Jurnal Masyarakat Madani Indonesia Indonesian Journal of Pharmacy and Natural Product Media Farmasi
Idha Kusumawati
Fakultas Farmasi Universitas Airlangga
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Journal : Media Farmasi

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Teknik Aplikasi Sampel Pada Pengujian Kuantitatif Kromatografi Lapis Tipis: Tinjauan Terhadap Area Dan Faktor Retensi Kusumawati, Idha; Primaharinastiti, Riesta; Rifqi Prasetyawan, Hanif
Media Farmasi Vol 20 No 2 (2024): Media Farmasi Edisi Oktober 2024
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v20i2.655

Abstract

Thin Layer Chromatography (TLC) is one of the most commonly used chromatographic techniques to identify compounds in mixtures and determine the purity of a compound. However, the crucial factor that is difficult to control, which can affect the accuracy of the results, is the stage of sample application. This study focused on investigating the effect of different techniques of sample application (manual and semi-automatic) on the area and retention factor (Rf) of the compound spot. Curcuminoid was used as a sample in this study. The manual sample application techniques used a 2μL capillary tube. The semi-automatic sample application techniques used a Camag Linomat-5 instrument with 0, 7, and 8 mm band lengths. After the development of the TLC plate using toluene: acetic acid (4:1, v/v)., the curcuminoid was separated into three spots: curcumin, demethoxycurcumin, and bisdemethoxycurcumin. A one-way ANOVA was used to analyze the spot area and Rf of the three compounds, followed by a Duncan test. The difference in sample application techniques causes differences in the size and shape of the initial sample spot, which affects the compound spot produced after developing of TLC plate. This study showed that the differences in application techniques cause significant differences in the Rf of the compound spot, but do not show significant differences in the compound spot area. Based on this research, optimizing the application techniques of samples is a critical stage and it is essential to optimize how to apply the sample on TLC plate, including the concentration, volume, shape, and size of the spot on TLC.
Co-Authors Abdul Rahman Abdul Rahman Achmad Toto Poernomo Amalia, Savira Rizky Meisya Ana Yuda Anang Teguh Eko Santoso Andi Hermansyah Aprelita Nurelli Dwiana Asri Darmawati Aty Widyawaruyanti Bambang Purwanto Bambang Tri Purwanto Dewanthy, Kyrana Sekar Dewi Isadiartuti Dewi Melani Hariyadi Dian Rahmawaty Diana Nurus S Diyah, Nuzul Wahyuning Dwi Setyawan Eka Pramyrtha Hestianah Ekapratista, Belinda Nabilah Esti Hendradi Estu Mahanani Dhilasari Fajar Jamaluddin Sandhori Fauzia Azzahra, Fauzia Fauzia, Alfi Nur Firmansyah Ardian Ramadhani Fitria, Anisa Lailatul Gamar, Fahmi Haitsami Ibnu Gemparing Bayu Wiyoto Hadi Poerwono Haliza, Nabila Nur Handayani, Rosita Hanif Rifqi Prasetyawan Hardiyanti, Merry Hari Basuki Notobroto Hari Setiono Harjono Suko Hermansyah, Andi Herra Studiawan Isnaeni Isnaeni Isnaeni Isnaeni Jusak Nugraha Laksmi, Farika Dyani Laksmi Lucia Tri Suwanti, Lucia Tri Mahalaksmi, Alika Sabrina Marcellino Rudyanto Maria Lucia Ardhani Dwi Lestari Mega Ferdina Suwito Muhammad Agus Syamsur Rijal Munandar, Tristiana Erawati Najlaa, Fatimah Ahla Neni Purwitasari Ni Made Mertaniasih Novian, Farah Kusuma Wardhani Nur Hayati Nurrosyidah, Iif Hanifa Paramita, Diajeng Putri Pattymahu, Charlyna Veronika Puspitasari Pradnyanantha, I Putu Bagus Pramesti, Ragil Putri Prasetyawan, Hanif Rifqi Pratama, Yusuf Alif Pratiwi Soesilawati Primaharinastiti, Riesta Primaharinastiti Purwitasari, Neni Puspitasari, Hanni Prihastuti Puspitasari, Hanni Prihhastuti Rakhmawati - - Ramadhani, Firmansyah Ardian Rania, Athaya Putri Retno Sari Retno Widyowati Rice Disi Oktarina Riesta Primaharinastiti Riesta Primaharinastiti, Riesta Rohana, Melissa Rohman Hadi Rohmania Rohmania Ruzainah Ali Salam, Anisa Rakhmania Samirah Samirah, Samirah Sandhori, Fajar Jamaluddin Sari Retno Sholika Sari, Trisiyana Siammita, Dhea Anansya Sri Subekti Subhan Rullyansyah Suciati Suciati Suciati Suciati Sudjarwo Sudjarwo Sugiyartono, . Sugiyartono, . Sukardiman Suprapto Maat Suprapto Maat Syailendra Mahatmaputra Tristiana Erawati Tutiek Purwanti Tutik Sri Wahyuni Wahjo Djatmiko Wahjo Dyatmiko Wahjo Dyatmiko Wardhana, Brahma Wisnu Kusuma Warsito, Mega Ferdina Wiwied Ekasari Yoes Prijatna Dachlan Yusuf, Helmy Yusuf, Helmy Yuwono Mochamad Zamrotul Izzah