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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Sains Peternakan Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia Jurnal Ternak Tropika Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal JURNAL ILMIAH PETERNAKAN TERPADU ARSHI Veterinary Letters Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat JURNAL KAJIAN VETERINER Jurnal Ilmu Peternakan Terapan Journal of Tropical Animal Science and Technology Agrinimal Jurnal Ilmu Ternak dan Tanaman Wahana Peternakan Jurnal Nukleus Peternakan Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Jurnal Penelitian Inovatif Jurnal Peternakan Lahan Kering Journal of Livestock and Animal Health (JLAH) Animal Agricultura Journal of Innovative and Creativity
Wilmientje Marlene Mesang Nalley
Fakultas Peternakan Universitas Nusa Cendana Kupang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PENGARUH LAMA EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS-MODIFIKASI DENGAN PENAMBAHAN KRIOPROTEKTAN (Effect of equilibration time on frozen sement quality of the duroc pig in tris diluents modification With..... Biqshierz V.W Koelima; Henderiana L.L. Belli; Johny N. Kihe; Wilmientje M. Nalley
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.5490

Abstract

The purpose of this study was to test the influence of the equilibration time on the frozen semen quality of duroc pig in modified tris-egg yolk with the addition of extracellular cryoprotectants. Semen is collected twice per week using massage methods from two duroc males aged ±2.5 years which in good health and have been trained in semen colection. The design used is a completely randomized design with 4 tests and 3 treatments T1: equilibration time of 1 hour, T2: equilibration time of 2 hours, T3: equilibration time of 3 hours at a temperature of 50C. Observed parameters were motility, viability, abnormality, MPU, and recovery rate (RR). The results showed that motility, viability, abnormalities, MPU, and RR were higher (P<0.05) at 2 hours of equilibration (25.63±3.15%, 55.86±5.41%, 4.92±0.53%, 56.69±5.64%, 36.61±4.49%) compared to 1 and 3 hours (20.00±3.54%, 39.28±9.89%, 7.93±0.76%, 40.21±9.39%, 28.57±5.05%) and (15.00±4.08%, 32.19±7.0%, 8.41±0.70%, 32.71±7.07%, 21.43±5.83%). It was concluded the motility of duroc pig spermatozoa in the tris-modified diluents after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the requirement of 40% spermatozoa motility for artificial insemination. It was concluded that the sperm motility of Duroc pig in tris-modified diluent after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the standard of 40% spermatozoa motility for artificial insemination.
KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS MODIFIKASI DENGAN WAKTU EKUILIBRASI YANG BERBEDA (The frozen sperm quality of duroc pig in modified tris diluent with different equilibration time) Selsiana Dongkot; Aloysius Marawali; Thomas M. Hine; Wilmientje M. Nalley
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.5491

Abstract

This study aimed to examine the quality of frozen sperm of duroc boar in modified Tris diluent with different equilibration time. Semen was collected from two duroc males and evaluated macroscopically and microscopically. Semen with motility 75%, concentration 200x106 cell/mL and abnormality <20% was diluted in Tris modified diluent, then held for two hours at room temperature (27-28ºC). The semen that has been in the holding time is then centrifuged at 2000 rpm for 15 minutes. The pellet from centrifuge were diluted again with modified Tris diluent + 3% glycerol. The diluted semen was packed in 0,50 mL mini straws and equilibrated according to the treatment equilibration time, namely P1: 1 hour equilibration time, P2: 2 hour equilibration time, P3: 3 hour equilibration time. After equilibration , it was frozen over liquid nitrogen vapor (-110ºC) at a distance of 5 cm for 10 minutes, then stored in liquid nitrogen (-196ºC) and left for 24 hours. Then thawing with warm water at 37ºC for 30 seconds in a water bath. The result showed that motility, viability, abnormality, intact plasma membrane and recovery rate were significantly higher (P<0,05) at two hour equilibration , namely 23.12%, 45.44%, 4.65%, 50,15%, 33.03% respectively compared to one and two hour equilibration time, namely 20%, 42.17%, 8.19%, 42.67%, 28.57% and 16,87%, 36.39%, 7.64%, 37.73%, 24.99% respectively. This study conclude that the equilibration time of two hours resulted in higher quality of frozen sperm of duroc boar than one and three hours.
Pengaruh Suplementasi Jenis Dedaunan Terfermentasi terhadap Kecernaan Serat Kasar dan Lemak Kasar terhadap Babi Fase Starter-Grower: The Effect of Suplementation of Fermented Foliar Types on The Digestibility of Crude Fiber and Crude Fat on Starter-Grower Phase Gerson Andrimus Naimasus; Wilmientje Marlene Nalley; I Made Suaba Aryanta
Jurnal Peternakan Lahan Kering Vol. 4 No. 3 (2022): September
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57089/jplk.v4i3.1277

Abstract

Tujuan dilaksanakan penelitian ini untuk mengetahui konsumsi dan kecernaan serat kasar dan lemak kasar dari ternak babi yang diberikann suplmentasi ransum dedaunan terfermentasi. Materi yang digunakan 12 ekor babi berumur antara 1-3 bulan dan dengan berat badan awal berkisar antara 5,35 sampai 14,55 kg (KV = 31,67%) dan Rancangan Acak Kelompok (RAK) dengan 4 perlakuan dan 3 ulangan. Perlakuan meliputi R0: diet basal R1: diet basal ditambah 5% tepung daun singkong fermentasi, R2: diet basal ditambah 5% tepung daun ubi jalar fermentasi, dan R3: diet basal ditambah 5% tepung daun kelor fermentasi. Hasil penelitian ini menunjukan perlakuan berpengaruh tidak nyata (P<0,05) terhadap variabel yang diteliti. Disimpulkan bahwa pemberian ransum suplementasi jenis dedaunan terfermentasi pada ternak babi mampu meningkatkan kecernaan serat kasar dan lemak kasar, akan tetapi belum mampu meningkatkan konsumsi serat kasar dan lemak kasar. The purpose of this study was to determine the intake and digestibility of crude fiber,and  crude fat,  of pigs fed fermented leafa ration supplementation. Material used were12 pigs aged between 1-3 months and with initial body weight ranging from 5.35 to 14.55 kg (KV = 31.67%) and Randomized Block Design (RBD) with 4 treatments and 3 replications. The treatments included R0: basal diet, R1: basal diet plus 5% fermented cassava leaf flour, R2: basal diet plus 5% fermented sweet potato leaf flour, and R3: basal diet plus 5% fermented moringa leaf flour. The results of this study showed no significant effect (P<0.05) on the variables studied. It was concluded that the supplementation of fermented foliage rations in pigs was able to increase the digestibility of crude fiber and crude fat, but had not been able to increase the intake of crude fiber and crude fat.  
PENGARUH KOMBINASI SUSU KACANG KEDELAI DAN PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SEMEN SAPI BALI (Effect of combination soy bean milk and citrate egg yolk liqid on the quality of bali cattle semen) Maria E.E. Fernandez; Wilmientje M Nalley; Franky M.S. Telupere; Thomas M. Hine
JURNAL NUKLEUS PETERNAKAN Vol 9 No 2 (2022): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i2.7949

Abstract

One of the diluent containing lipoproteins and lecithins that function in maintaining and protecting the integrity of the plasma membrane of spermatozoa from cold shock is soy milk. The purpose of this study was to determine the effect of the combination of soy bean milk and egg yolk citrate liqud on the quality of liquid semen of bali cattle. The material used in this study was fresh semen of 3 year old bali cattle. Semen was diluted with egg yolk citrate diluent with a combination of soy bean milk, namely T0: 100% citrate egg yolk (CEY); T1: CEY 75%+soy bean milk (SBM) 25%; T2: CEY 50%+SBM 50%; T3: CEY 25%+SBM 75%; and T4: 100% SBM. Liqud semen was stored at a temperature of 3-5oC. The parameter studied were motility, viability, abnormality and liveability of spermatozoa. The research data ware analyzed using an analysis of variance (ANOVA) and followed by the multiple range test. The results showed that T1 significantly difference (P<0.05) from other treatments, with motility of 42.40±2.50%, viability 52.15±6.31%, abnormality 6.44±0.84%, and liveability 6.00±0.00 days. From these result it could be concluded that the combination of 25% soy bean milk and 75% egg yolk citrate liqud was the best treatment, and spermatozoa can be stored until six days.
PENGARUH PENAMBAHAN LAKTOSA DI DALAM PENGENCER TRIS DAN SITRAT TERHADAP KUALITAS SEMEN CAIR SAPI ANGUS (The effect of additional lactose in tris and citrate diluents on the quality of liquid semen of angus cattle) Satria J.S. Rihileo; Kirenius Uly; Wilmientje M. Nalley; Hendriana L.L. Belli
JURNAL NUKLEUS PETERNAKAN Vol 10 No 1 (2023): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v10i1.7952

Abstract

Lactose is the only carbohydrate found in milk and serves as an additional source of energy and protects the plasma membrane of spermatozoa from damage due to cold shock during storage at low temperatures. The purpose of this study was to examine the effect of adding lactose in tris and citrate diluents on the quality of liquid semen. One 3 year old angus cow in healthy condition. Semen was diluted with diluent P0: Tris-egg yolk (T-EY) 100%, P1: Citrate-egg yolk (C-EY) 100%, P2: T-EY 50 %+C-EY 50%, P3: T-EY 50%+ C-EY 50%+Lactose 1.5g, P4: T-EY 50%+ C-EY 50%+Lactose 3.0g, P5: T- EY 50%+ C-EY 50%+Lactose 4.5g. After diluted cement is stored at a temperature of 3-5oC. Semen was evaluated post dilution and every 24 hours of storage for motility, viability, abnormalities and survival of spermatozoa. The data were analyzed using analysis of variance (ANOVA) and Duncan's test. The results of this study showed that the P3 (P>0.05) treatment was the best compared to other treatments, namely the percentage of spermatozoa motility values of 43.50±2.23%, spermatozoa viability was 53.07±3.36%, spermatozoa abnormalities were 8.01±2.14%, and spermatozoa survival was 6.60.00±0.54 days. The results of this study indicate that using a combination of T-EY and C-EY diluents with the addition of 1.5g lactose is the best diluent in maintaining the quality of liquid semen of angus cattle.
Spermatozoa quality of Landrace Boar in Beltsville thawing solution diluent with various levels of moringa leaf extract Wirona Adu; Thomas Hine; Aloysius Marawali; Wilmientje Marlene Nalley
Jurnal Ilmu Peternakan Terapan Vol 7 No 1 (2023): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v7i1.4027

Abstract

This study aims to find the best level of sun-dried Moringa leaf extract (SDMLE) in Beltsville Thawing Solution (BTS) diluent to preserve the spermatozoa of Landrace boars. Semen from a two-year-old Landrace boar was collected using the massage method. Semen with spermatozoa motility ≥70% and spermatozoa abnormalities <20% was diluted with BTS (P0, control), BTS + 3% SDMLE (P1), BTS + 6% SDMLE (P2), BTS + 9% SDMLE (P3), and BTS + 12% SDMLE (P4). Storage of liquid semen using a cool box with a temperature of 15-20oC, while evaluating the quality of semen every 8 hours for spermatozoa motility, viability, abnormality, and longevity. The results showed that spermatozoa stored for 40 hours in BTS + 3% SDMLE (P1) diluent had higher quality (P<0.05) than the other four diluents, namely motility (54.00%), viability (61.16%), abnormality (3.62%), and longevity (45.77%). The results of this study concluded that 3% SDMLE added to BTS diluent was the best level for the preservation of spermatozoa in landrace boars.
Natural honey supplementation in Tris-egg yolk diluent as Carbohydrate source replacing Fructose in Etawah Crossbred Goat semen Yustiani Yuliana Bette; Wilmientje Marlen Nalley; Thomas MataHine
JURNAL ILMIAH PETERNAKAN TERPADU Vol 12, No 1 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i1.p49-62

Abstract

This study aims to identify the substitution of native honey as an energy source to replace fructose in Tris-KT diluent to improve the quality of spermatozoa of Etawah crossbreed goats (PE). The study was conducted at the Nekamese Vocational School and the Wiliams and Laura Foundation Biological and Reproductive Laboratory for 2 months. In this study, four PE male goats were used as a source of semen. Cement is accommodated twice a week. The semen obtained was evaluated macroscopically and microscopically then diluted with T-KT diluent with the addition of honey divided into P0 (control), P1 (T-KT + 0.25 w/v honey), P2 (T-KT + 0.50 w/v honey) and P3 (T-KT + 0.75 w/v honey). After dilution the cement is stored at a temperature of 3-5°C.  Semen is evaluated post-dilution and post-storage every 24 hours, until the movement of spermatozoa is at least 40%. The variables tested were motility and viability of spermatozoa. P3 provides the best results in maintaining cement quality. The results showed that honey substitution in Tris-KT diluent exerted a marked effect on the motility and viability of spermatozoa (P<0.05) during storage.
Pengaruh Substitusi Tepung Jagung dengan Batang Pisang Terfermentasi terhadap Konsumsi, Kecernaan Protein dan Energi pada Ternak Babi Duroc Fase Grower: Effect of Corn Flour Substitution with Banana Stem Fermentation on Consumption, Protein Digestability and Energy in Duroc Grower Pig Maria Gordince Owa; Tagu Dodu; Wilmientje Marlene Nalley
Jurnal Peternakan Lahan Kering Vol. 6 No. 1 (2024): Maret
Publisher : Universitas Nusa Cendana

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Abstract

The purpose of research is to test the influence of corn flour substitution with fermented banana rodsagainst consumption and digestion of protein, consumption and digestion of energy in pig cattle durocphase grower. The material used is 12 pigtails duroc phase grower that has a starting weight of 45-55kg with an average of 51.25 kg (KV=7,02%). This research uses a complete random design (RAL)with 4 treatments and 3 repeats. The treatment used is P0: the bed without the mixture of fermentedbanana stem, P1: the ration contains 5% fermented banana rod, P2: the ration contains 10% offermented banana stems and P3: the ration contains 15% of fermented banana stems. The measuredvariable is the consumption and digestion of protein and energy. Analysis results show the influence ofcorn flour substitution with fermented banana stems of 5, 10 and 15 intangible effects (P>0,05) againstprotein ingestion, energy consumption and energy ingestion, and real effect (P<0,05) against proteinconsumption. It is concluded that the substitution of corn flour with fermented banana stems up to 15%level increases protein consumption but up to 15% level increases protein consumption and lowersrational consumption, protein ingestion, energy consumption and energy in pigs doruc phase grower. Tujuan penelitian adalah untuk menguji pengaruh substitusi tepung jagung dengan batang pisangterfermentasi terhadap konsumsi dan kecernaan protein, konsumsi dan kecernaan energi pada ternakbabi duroc fase grower. Materi yang digunakan adalah 12 ekor ternak babi duroc fase grower yangmemiliki berat badan awal 45-55 kg dengan rataan 51,25 kg (KV=7,02%). Penelitian ini menggunakanrancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan yang digunakan adalahP0: ransum tanpa campuran batang pisang terfermentasi, P1: ransum mengandung 5% batang pisangterfermentasi, P2: ransum mengandung 10% batang pisang terfermentasi dan P3: ransum mengandung15% batang pisang terfermentasi. Variabel yang diukur adalah konsumsi dan kecernaan protein danenergi. Hasil analisis menunjukkan pengaruh substitusi tepung jagung dengan batang pisangterfermentasi sebanyak 5, 10 dan 15 berpengaruh tidak nyata (P>0,05) terhadap kecernaan protein,konsumsi energy dan kecernaan energi, dan berpengaruh nyata (P<0,05) terhadap konsumsi protein.Disimpulkan bahwa substitusi tepung jagung dengan batang pisang terfermentasi hingga level 15%meningkatkan konsumsi protein namun hingga level 15% meningkatkan konsumsi protein danmenurunkan konsumsi ransum, kecernaan protein, konsumsi energy dan kecernaan energy pada ternakbabi doruc fase grower.  
Suplementasi Tepung Daun Asam dalam Ransum terhadap Pertambahan Ukuran Linear Tubuh dan Prediksi Bobot Badan Ternak Babi Landrace Fase Grower: Tamarind Leaf Flour Supplementation in the ration on the increase in linear body size and prediction of body weight for Landrace Pigs in the Grower Phase Sepriyono Aklili; W. Marlene Nalley; Tagu Dodu
Jurnal Peternakan Lahan Kering Vol. 6 No. 1 (2024): Maret
Publisher : Universitas Nusa Cendana

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Abstract

This study aims to examine the effect of tamarind leaf powder supplementation on (Tamarindus indical L.)supplementation in the basal ration on the increase in linear body size and prediction of body weight of pigs.Twelve landrace castration male pigs aged 3-4 months with initial body weight of 35.5-64 kg (CV=23.65%)were used as research animals. The design used was a randomized block design with 4 treatments and 3replications. The treatments tested were T0: basal ration (control), T1: basal ration (BR) + 2.5% tamarind meal(TM), T2: BR + 5% TM, T3: BR + 7.5% TM. The variables measured were body length, schest circumference,height, predicted body weight. The results of the analysis showed that the treatment had no significant effect(P>0.05) on body length, chest circumference, height, prediction of body weight. It can be concluded that thesupplementation of tamarind leaf powder in the basal ration up to 7.5% has not had an effect on body length,chest circumference, height and prediction of body weight. Penelitian ini bertujuan untuk menguji pengaruh suplementasi tepung daun asam (Tamarindus indical L.) dalamransum basal terhadap pertambahan ukuran linear tubuh dan prediksi bobot badan ternak babi. Ternak babijantan kastrasi peranakan landrace sebanyak 12 ekor berumur 3-4 bulan dengan berat badan awal 35,5-64 kg(KV=23,65%) digunakan sebagai ternak penelitian. Rancangan yang digunakan adalah rancangan acakkelompok dengan 4 perlakuan dan 3 ulangan. Perlakuan yang diuji adalah R0: ransum basal (kontrol), R1:ransum basal (RB) + 2,5% tepung daun asam (TDA), R2: RB + 5% TDA, R3: RB + 7,5% TDA. Variabel yangdiukur adalah panjang badan, lingkar dada, tinggi badan, prediksi bobot badan. Hasil analisis menunjukkanbahwa perlakuan tidak berpengaruh nyata (P>0,05) terhadap panjang badan, lingkar dada, tinggi badan, prediksibobot badan. Dapat disimpulkan bahwa suplementasi tepung daun asam dalam ransum basal hingga 7,5% belummemberikan pengaruh terhadap panjang badan, lingkar dada, tinggi badan dan prediksi bobot badan.  
Pengaruh Penambahan Ekstrak Daun Kemangi (Ocimum Sanctum) Dalam Pengencer Tris Kuning Telur Terhadap Kualitas Semen Babi Landrace Selama Penyimpanan: The Effect Of Adding Basil Leaf Extract (Ocimum Sanctum) In Tris-Egg Yolk Diluent To The Semen Quality Of Landrace Pig During Storage Juliyanti Stefania Salem; Henderian L. L. Belli; Kirenius Uly; Wilmientje M. Nalley
Jurnal Peternakan Lahan Kering Vol. 6 No. 1 (2024): Maret
Publisher : Universitas Nusa Cendana

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Abstract

The purpose of this study was to determine the effect of adding basil leaf extract (BLE) in Tris-eggyolk diluent (T-EY) on semen quality of landrace pig during storage. The semen used comes from landracepig collected using the massage method. Good quality semen with motility 70%, concentration 150×106and abnormality 20% were diluted in T-EY diluent with the addition of BLE for treatment. The treatmentwas designed with CRD with the following treatment; P0 : T-EY + 0% BLE; P1 : T-EY + 1.5% BLE; P2 : T-EY + 3% BLE and P3 : T-EY + 4.5%% BLE each treatment was repeted five times. After dilution, the spermis checked for motility, viability and abnormalities, and then stored in a cool box. Seed scoring is repeatedevery 8 hours until motility reaches 40%. The variabel tested were motility, viability, abnormality and spermsurvival during storage. The results showed that the addition of basil leaf extract to the Tris-egg yolk had nosignificant effect (P > 0,05) on sperm motility, viability and survival of landrace pig during storage. It can beconcluded that the addition of basil leaf extract to the Tris-egg yolk diluent did not produce a good seedpreservation response. Tujuan penelitian ini untuk mengetahui pengaruh penambahan ekstrak daun kemangi (EDk) dalampengencer Tris kuning telur (T-KT) terhadap kualitas semen babi landrace selama penyimpanan. Semen yangdigunakan berasal dari babi landrace, yang ditampung dengan metode masagge. Semen yang berkualitas baikdengan motilitas 70% konsentrasi 150×106 dan abnormalitas 20% dienceran dalam pengencer T-KTdengan penambahan Edk sebagai perlakuan. Perlakuan dirancang menggunakan RAL dengan perlakuansebagai berikut; P0 : T-KT + 0% EDk; P1 : T-KT + 1,5% EDk; P2 : T-KT + 3% EDk dan P3 : T-KT + 4,5%EDk setiap perlakuan diulang sebanyak 5 kali. Setelah diencerkan semen dievaluasi terhadap motilitas,viabilitas dan abnormalitas, selanjutnya disimpan dalam cool box evaluasi semen dilakukan kembali setiap 8jam hingga motilitas mencapai 40%. Variabel yang di uji adalah motilitas, viabilitas, abnormalitas dan dayatahan hidup spermatozoa selama penyimpanan. Hasil penelitian menunjukkan bahwa penambahan ekstrakdaun kemangi dalam pengencer Tris kuning telur berpengaruh tidak nyata (P > 0,05) terhadap penurunanmotilitas, viabilitas dan daya tahan hidup spermatozoa babi landrace selama penyimpanan. Dapatdisimpulkan bahwa penambahan ekstrak daun kemangi dalam pengencer Tris kuning telur tidak memberikanrespon yang baik dalam preservasi semen.  
Co-Authors A. Kaka Adyanto Nessy Banamtuan Agus Saputra Agustinus Ridlof Riwu Agustinus Ridlof Riwu Aleksander Kaka Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Anak Agung Gede Sugianthara Arnold Elyazar Manu Arnold Elyazar Manu Arnoldianus Harbin Asael Ebson Nabut B Purwantara B Purwantara B. Purwantara Bambang Purwantara Biqshierz V.W Koelima Burhanuddin * Burhanuddin burhanuddin Daniel Kurniawan Tulle Dani Tulle David Agustinus Nguru Demianus Ferdinand Souhoka Dodu, Tagu Fanny Tesalonika Hilly Faustus Jusing Fian K. Djita Foeh, Nancy D. F. K. Franky M S Telupere Franky M.S. Telupere G Semiadi G. Semiadi Gaina, Cynthia Dewi Gerson Andrimus Naimasus GONO SEMIADI Henderian L. L. Belli Henderiana Belli Henderiana Laura Louisa Belli Hendriana L.L. Belli Herdis . Heri Armadianto Heri Armadianto I Made Adi Sudarma I Made Suaba Aryanta Imanuel Benu Imanuel Benu J A Tamoes Jernih Blegur Johny N. Kihe jourvan manasye marabijala Juliyanti Stefania Salem kirenius uly Kirenius Uly Kirenius Uly Kristina Enjelina Jigha Kune , P. Lawa, Alvrado Bire Lombo, Jelittron Putra Lote, Nyongki Mallo, Agustinus Maman Surachman Maman Surachman Mandanil Arisma Leo Manibak, Maskrim Letlang Mardemus Misa Maria E.E. Fernandez Maria Elisabeth Anjenita Ampur Maria Epifania Barek Maria F. T. Leyn Maria G. Mega Maria Gordince Owa Maria M. Bria Maria Meldiana Jemamu Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Maxs Urias Ebenheizer Sanam Melianus Fafo Michel Johan Matatula MUHAMMAD RIZAL N.G.A Mulyantini S.S Ni Gusti Ayu Mulyantini Ni Made Paramita Setyani Ni Nengah Suryani Ni Nengah Suryani Nuba, Maria Deran Papituan, Mardan Laes Paskalis Baylon Petrus Kune Petrus Kune Petrus Kune Petrus Kune R Handarini R I Arifiantini R. Handarini R. Handarini R. Iis Arifiantini R.I. Arifiantini Reni Ratni Dapawole RI Arifiantini, RI Ristika Handarini Riwu , R. M. J. Riwu, Ronny Markus Jami Rosnah, Upik Syamsiar Rut Listyadi Rut Riwu Ratu Samuel Alexsander Singga Satria J.S. Rihileo Selsiana Dongkot Sepriyono Aklili Setyani , N. M. P. Simon Edison Mulik Sofia Marlize Soleman Manaze Weiri Sinda T L Yusuf T.L. Yusuf Tagu Dodu Tarsisius Considus Tophianong, Tarsisius Considus Theresia Oktaviana Laka Thomas Mata Hine Tree May Thiessen Outang Tuty Laswardi Yusuf Twen O. Dami Dato Ulrikus R. Lole Uly , K. Wirona Adu Yanna Nailla Zulfa Yoana D. Fransiska Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette