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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Veteriner Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production ARSHI Veterinary Letters Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Ilmu Peternakan Terapan Jurnal Nukleus Peternakan Jurnal Peternakan Lahan Kering JIA (Jurnal Ilmiah Agribisnis) : Jurnal Agribisnis dan Ilmu Sosial Ekonomi Pertanian
Wilmientje Marlene Mesang Nalley
Fakultas Peternakan Universitas Nusa Cendana Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Social Sciences Veterinary

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PENGARUH PEMBERIAN EKSTRAK HIPOFISA DAN PROBIOTIK ABG-O PADA ANAK BABI PERSILANGAN TERHADAP PENINGKATAN BERAT BADAN SAPIH, UKURAN LINEAR TUBUH DAN METABOLIT DARAH (The effect of giving pituitary and probiotic extracts abg-o to piglets crosses.....) Mardemus Misa; Wilmientje M Nalley; Thomas Mata Hine; Twenfosel Dami Dato; Maritje A Hilakore; Mariana Nenobais; Aloysius Marawali
JURNAL NUKLEUS PETERNAKAN Vol 7 No 1 (2020): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i1.2256

Abstract

The purpose of this study was to determine the effect of giving pituitary extract (EH) and probiotics ABG-O on piglets crossing to increase weaning weight, linear size of body and blood metabolites. The research material was a crossbred of landrace and duroc aged 2 weeks, as many as 24 birds. The experimental design was a completely randomized design with three treatments and eight replications. The three treatments are P0 (control), P1 (EH injection 1 mL), and P2 (injection EH 1 mL + probiotic ABG-O 2 mL). EH injections are carried out every 5 days and administration of probiotics is given orally using spoit every day until the piglets are weaned. The parameters observed were weight gain, linear size of body and blood metabolites. This data was analyzed by ANOVA and continued with Duncan test. The results of this study indicate that the average daily weight gain of crossbred piglets in treatments P0, P1, and P2 are 0.11; 0.13; 016 kg, body length (0.40; 0.45; 0.44 cm), chest circumference (0.29; 0.27; 0,30 cm), and shoulder height (0.25; 0.24; 0.25 cm). Blood glucose levels (117.31; 112.85; 117.25) and total plasma protein (4.60; 4.90; 4.80). The results of the statistical analysis showed that the EH and ABG probiotic treatments affected the UN (P<0.05) but did not affect (P> 0.05) the size of the linear body and blood metabolites. It can be concluded that the administration of pituitary extracts and ABG probiotics can increase the weaning weight but does not affect the linear size of the body blood metabolites and total protein plasma of crossing piglets.
PENGARUH PENAMBAHAN VIRGIN COCONUT OIL DALAM PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI SELAMA PRESERVASI (Infiluence addition virgin coconut oil in tris egg yolk on the quality of bali bull spermatozoa during preservation) Jernih Blegur; Wilmientje M Nalley; Thomas Mata Hine
JURNAL NUKLEUS PETERNAKAN Vol 7 No 2 (2020): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i2.2997

Abstract

The purpose of this study was to examine the effect of adding various doses of virgin coconut oil to the quality of bali bull spermatozoa during preservation. Semen is accommodated twice a week using an artificial vaginal method from two 3-4 year old bali bull with normal body condition and reproductive organs. Semen with motility> 70%, concentration> 1000 × 106, and abnormality <15% was diluted with Tris-egg yolk (T-KT) diluent added with virgin coconut oil (VCO) at a concentration of: 0% (T0), 2 % (T1), 4% (T2), and 6% (T3). The diluted semen was stored at 3-5ᶱC. Evaluated every 24 hours for motility, viability, abnormalities and vitality of spermatozoa up to a motility of at least 40%. The results showed that semen diluted with T-EY with 6% VCO (T3) had a higher quality (P <0.05) compared to the other three treatments, with motility reaching (43.10 ± 2 , 83%), viability (48.68 ± 1.59%), abnormalities (5.92 ± 0.90%) and survival (6.00 ± 0.00 days). The conclusion of this study is the addition of 6% virgin coconut oil in Tris-egg yolk diluent is more effective in maintaining the quality of bali cattle liquid semen.
PENGARUH PENAMBAHAN SARI WORTEL DALAM PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING BLIGON (The effect of carrot juice supplementation in citrate - egg yolk extender on spermatozoa quality of bligon goat) Maria Epifania Barek; kirenius uly; Thomas Mata Hine; Wilmientje Marlene Nalley; Henderiana L.L. Belli
JURNAL NUKLEUS PETERNAKAN Vol 7 No 2 (2020): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i2.3152

Abstract

This study aims to determine the effect of adding various doses of carrot juice into citrate-egg yolk diluents (C-EY) on bligon buck semen quality. Semen was collected twice a week using an artificial vaginal method of three buck with normal body condition and reproductive organs. Semen was 70% of motility, konsentration ≥1000x106mL and abnormality ≤ 15 diluted with C-EY added with carrot juice at a concentrations: 0% (CJ-0), 10% (CJ-10), 12.5% (CJ-12,5), 15% (CJ-15), 17.5% (CJ-17,5), and 20% (CJ-20), and stored in a refrigerator at 3-5o C. The quality of spermatozoa was evaluated every 24 hours. The Variables measured were spermatozoa motility, viability, and abnormalities. The results showed that spermatozoa preserved by C-EY supplemented by 17.5% carrot juice (CJ-17.5) had a higher quality (P<0.05) compared to the other four treatments, with motility (49.04 ± 1.0%), viability (55.70 ± 1.22%), dan abnormalities (4.57 ± 0.88 %) on the 4th day of storage. This study concludes that 17.5% carrot juice into citrate eggyolk extender improves the bligon buck semen
KUALITAS SPERMATOZOA KAMBING BLIGON DALAM PENGENCER TRIS KUNING TELUR DENGAN PENAMBAHAN BERBAGAI LEVEL EKSTRAK KULIT BUAH NAGA Maria F. T. Leyn; Henderiana Belli; Wilmientje Marlene Nalley; Petrus Kune; Thomas Mata Hine
JURNAL NUKLEUS PETERNAKAN Vol 8 No 1 (2021): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i1.4230

Abstract

The purpose of this study was to examine the effect of adding various concentrations of dragon fruit peel extract (DFPE) in Tris-egg yolk diluent (TEY) on the quality of bligon goat spermatozoa. Semen was collected twice a week using the artificial vaginal method from three year old bligon male goats with normal body conditions and reproductive organs. After the macroscopic and microscopic evaluation, good quality semen with a sperm motility of > 70%, a concentration of > 800 x 106 mL-1, and abnormality of < 15 %, was diluted with TEY + DFPE at a concentration of 0% (P0), 2% (P1), 4% (P2), 6% (P3), 8% (P4), dan 10% (P5). The semen which still had a motility ≥ 70% was then stored at a temperature of 3-5oC. Evaluation of the quality of spermatozoa was carried out every 24 hours until the motility decrease to 40%. The results showed that from day 1 to day 5 of preservation, spermatozoa preserved in TEY + 6% DFPE diluent (P3) had higher motility and viability (P<0,05) than other treatments. The abnormalities of spermatozoa until the 5 day of preservation were below 6%, but showed a significant difference (P<0,05) between treatments that received DFPE suplemention with control (P0). It was concluded that the addition of DFPE in TEY up to 6% could reduce the decrease in sperm quality of bligon goats during in vitro preservation.
PENGARUH PENAMBAHAN EKSTRAK BAWANG MERAH (Allium cepa) DALAM PENGENCER TRIS - KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI PADA PENYIMPANAN IN VITRO (The effect of additional onion (Allium cepa) extract in tris - egg yolk extender on the sperm... Fian K. Djita; Wilmientje M. Nalley; Thomas M. Hine; Aloysius Marawali
JURNAL NUKLEUS PETERNAKAN Vol 8 No 2 (2021): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i2.4304

Abstract

The purpose of this study was to determine the effect of adding onion extract (OE) to Tris-egg yolk (T-EY) diluent on the quality of bali bulls spermatozoa. The semen of three bali bulls aged 3-4 years were collected twice a week using the artificial vaginal method. Semen with good quality, motility 79%, viability 84.63%, abnormality 2.93% diluted with T-EY diluent with the addition of OE: T0: 0%; T1 1%; T2: 2%; T3: 3%; T4: 4%; and T5: 5%. The diluted semen was preserved in a refrigerator at 3-5 °C and evaluated for the quality of the spermatozoa every 24 hours. The results showed that the addition of OE in T-EY diluent had a positive effect on the quality of bali bulls spermatozoa. The addition 3% of OE (T3) in T-EY diluent resulted in higher spermatozoa quality (P <0.05) than the other treatments, namely motility 42.01%, viability 48.74%, and abnormalities 4.13% on the sixth day of preservation. It was concluded that the addition of onion extract in Tris - egg yolk diluents could improve the quality of bali bulls spermatozoa, with the best level of onion extract being 3%.
PENGARUH WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM TERMODIFIKASI (Effect of equilibration time on the quality of landrace boar frozen semen in modified durasperm extender) Sofia Marlize; Thomas M. Hine; Wilmientje M. Nalley
JURNAL NUKLEUS PETERNAKAN Vol 8 No 2 (2021): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i2.4867

Abstract

This study aims to determine the effect of equilibration time on the quality of landrace spermatozoa both pre- and post-freezing and to find the best equilibration time. The materials used is fresh semen collected from a 2,5 years old landrace boar. Collecting was carried out twice per week using the glove hand method. Semen of good quality (sperm motility ≥70%, sperm concentration ≥200 x 106 cells/ml, and percentage of sperm abnormalities ≤15%) was diluted with durasperm that had been modified with lontar fruit juice, while the cryoprotectants used glycerol and sucrose. Holding time was carried out for 2 hour at 27-28°C, centrifuged at 2000 rpm for 15 minutes, and the sperm sediment was diluted with the same diluent. After that, the liquid semen was packed in a 0.5 mL straw and equilibrated at 3-5°C for 1 (P1), 2 (P2), or 3 hours (P3). The freezing of semen was carried out on the surface of liquid nitrogen at a distance of 5 cm for 10 minutes, and then stored in a liquid nitrogen container. Thawing is done by placing the frozen semen straws in warm water (37°C) for 30 seconds. The research data were analyzed with analysis of variance and continued with the Duncan test. The results showed that the equilibration time at 2 hours (P2) gave the best results (P<0,05) with the mean percentage of motility, viability, abnormality, intact plasma membrane and recovery rate respectively were 30,62%, 58,21%, 4,68%, 58,94% and 43,74%. It was concluded that the equilibration time did not affect the quality of pre-freezing spermatozoa, but it did affect the quality of post-freezing spermatozoa with the best equilibration time to maintain the quality of frozen semen of landrace boar was 2 hours.
Blood AST and ALT profile of Sumba Ongole cattle Cynthia Dewi Gaina; Maxs U.E. Sanam; Wilmientje M.M. Nalley; Imanuel Benu; Agus Saputra
ARSHI Veterinary Letters Vol. 4 No. 1 (2020): ARSHI Veterinary Letters - Februari 2020
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.793 KB) | DOI: 10.29244/avl.4.1.17-18

Abstract

A study was carried out in one of Indonesia native cattle, Sumba Ongole (SO) Cattle for determination of statuses of various biochemical parameters, aspartate aminotransferase (AST or SGOT) and alanine aminotransferase (ALT or SGPT). Apparently blood from randomly selected cattle (n= 36) were collected aseptically. Biochemical analysis of AST and ALT was performed on UPT. Laboratorium Kesehatan, Kupang, NTT. The results showed that the variations of parameters were not significant (p>0.05) between male and female SO cattle. The AST/SGOT (142.56±20.55 U/l) was slightly higher in female compared with the male (137.00±16.24 U/l), while level of ALT/SGPT were 46.44±10.99 U/l in male and 46.41 ± 10.23 U/l in female. Although, the result was not significant (p>0.05), level of AST and ALT in SO cattle was higher compared to standard which is 60-125 U/l for AST and 6.9-35 U/l for ALT. Results showed possibility of seasonal effect on AST/ SGOT and ALT/SGPT level in male and female SO cattle.
PENGARUH SUBTITUSI SARI BUAH SEMANGKA (Citrullus lanatus) DALAM PENGENCER SITRAT- KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI (Effect of watermelon juice supplementation in citrate - egg yolk extender on spermatozoa quality of bali bulls) Maria M. Bria; Wilmientje M. Nalley; Johny N. Kihe; Thomas M. Hine
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.4393

Abstract

This study aims to determine the effect of adding watermelon juice (WJ) into citrate-egg yolk (C-EY) diluents on the spermatozoa quality of bali catlle. Semen was collected twice a week using an artificial vaginal method of three bali bulls with normal body condition and reproductive organs. Semen that has motility > 70%, concentration >1000 x 106 and abnormalities ≤ 20 % was used in this study. Semen then divided into five treatments: C-EY 100% (T0), C-EY-25% WJ (T1), C-EY-50% WJ (T2), C-EY-75% WJ (T3), WJ 100% (T4) and then stored in a refrigerator at temperature 3-5oC. The quality of spermatozoa was evaluated every 24 hours for sperm motility, viability, and abnormalities. The results showed that spermatozoa diluted with S-KT supplemented with 75% WJ (T3) had a higher quality (P<0,05) compared to the other treatments, with motility 40,53 %, viability 47,35%, and abnormalities 4,21% on the 6th day of preservation. It was concluded that the addition of WJ to C-EY diluent was able to maintain the quality of bali bull spermatozoa, with the best SBS concentration is 75%.
PENGARUH PERBEDAAN WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM MODIFIKASI DENGAN AIR BUAH LONTAR (Effect of different equilibration time on the quality of landrace boar frozen semen on modification diluent.... Maria G. Mega; Wilmientje M. Nalley; Aloysius Marawali; Henederiana L. L. Belli
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.4988

Abstract

An experiment was carried out to recognize the effect of long equilibration time on the quality of spermatozoa and to obtain optimal equilibration time in the process cryopreservation of landrace pig semen. Semen was obtained from a landrace boar aged 2-3 years using glove hand method. The semen was than evaluated macroscopically and microscopically, good quality semen was diluted in a basic diluent, than kept for two hours at a temperature of 27-28oC. The semen were then centrifuged at 2000 rpm for 15 minutes, the supernatant was discarced and the pellets was re-diluted into basic diluent with a ratio of 1:1. Then, semen was diluted in treatment diluent with modified durasperm + 6% lactose + 4% glycerol with a concentration of 500 x 106/0.5 mL. Furthermore, the semen was packaged in 0.5 ml straws, then equilibrated at a temperature of 3-5oC appropriate to treatment, with 1 hour equilibration (T1), 2 hours equilibration (T2) and 3 hours equilibration (T3). After equilibration, the straws was frozen horizontally 5 cm above the liquid N2 vapor for 10 minutes at a temperature of ± -110oC and then stored in a liquid N2 container (-196oC). To test frozen semen, it was done by stored frozen semen at 37oC for 30 seconds. The results showed that the highest quality of spermatozoa (P <0.05) was found in T2 and T3 when compared to T1 (P> 0.05), with the motility value was T2: 28.13 ± 2.39%; T3: 25.00 ± 4.08%; and T1 18,15±3,23%. It can be concluded that 2 hours and 3 hours equilibration time was able to maintain the quality of frozen semen landrace boars and the statistically was not significant.
PENGARUH LAMA EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS-MODIFIKASI DENGAN PENAMBAHAN KRIOPROTEKTAN (Effect of equilibration time on frozen sement quality of the duroc pig in tris diluents modification With..... Biqshierz V.W Koelima; Henderiana L.L. Belli; Johny N. Kihe; Wilmientje M. Nalley
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.5490

Abstract

The purpose of this study was to test the influence of the equilibration time on the frozen semen quality of duroc pig in modified tris-egg yolk with the addition of extracellular cryoprotectants. Semen is collected twice per week using massage methods from two duroc males aged ±2.5 years which in good health and have been trained in semen colection. The design used is a completely randomized design with 4 tests and 3 treatments T1: equilibration time of 1 hour, T2: equilibration time of 2 hours, T3: equilibration time of 3 hours at a temperature of 50C. Observed parameters were motility, viability, abnormality, MPU, and recovery rate (RR). The results showed that motility, viability, abnormalities, MPU, and RR were higher (P<0.05) at 2 hours of equilibration (25.63±3.15%, 55.86±5.41%, 4.92±0.53%, 56.69±5.64%, 36.61±4.49%) compared to 1 and 3 hours (20.00±3.54%, 39.28±9.89%, 7.93±0.76%, 40.21±9.39%, 28.57±5.05%) and (15.00±4.08%, 32.19±7.0%, 8.41±0.70%, 32.71±7.07%, 21.43±5.83%). It was concluded the motility of duroc pig spermatozoa in the tris-modified diluents after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the requirement of 40% spermatozoa motility for artificial insemination. It was concluded that the sperm motility of Duroc pig in tris-modified diluent after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the standard of 40% spermatozoa motility for artificial insemination.
Co-Authors A. Kaka Agus Saputra Aleksander Kaka Alfred Nubatonis Aloysius Marawali Anak Agung Gede Sugianthara Arnoldianus Harbin Asnawati Is, Asnawati Is B Purwantara B Purwantara B. Purwantara Bambang Purwantara Biqshierz V.W Koelima Bire Lawa, Alvrado Burhanuddin * Burhanuddin burhanuddin Demianus Ferdinand Souhoka Fian K. Djita Fikriman, Fikriman Franky M S Telupere G Semiadi G. Semiadi Gaina, Cynthia Dewi GONO SEMIADI Henderian L. L. Belli Henderiana Belli Hendriana L.L. Belli Herdis . Heri Armadianto Heri Armadianto I Made Adi Sudarma Imanuel Benu J A Tamoes Jernih Blegur Johny N. Kihe Juliyanti Stefania Salem kirenius uly Kirenius Uly Kirenius Uly Maman Surachman Maman Surachman Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria F. T. Leyn Maria G. Mega Maria Gordince Owa Maria M. Bria Mariana Nenobais Maritje Aleonor Hilakore Maxs Urias Ebenheizer Sanam Melianus Fafo Michel Johan Matatula MUHAMMAD RIZAL N.G.A Mulyantini S.S Petrus Kune Petrus Kune Petrus Kune R Handarini R I Arifiantini R. Handarini R. Handarini R. Iis Arifiantini R.I. Arifiantini Reni Ratni Dapawole RI Arifiantini, RI Ristika Handarini Satria J.S. Rihileo Selsiana Dongkot Sepriyono Aklili Setiono Setiono Sofia Marlize Soleman Manaze Weiri Sinda T L Yusuf T.L. Yusuf Tagu Dodu Thomas Mata Hine Tree May Thiessen Outang Tuty Laswardi Yusuf Twen O. Dami Dato Ulrikus R. Lole Wirona Adu Yohanis Umbu Daku Waluwanja