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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Sains Peternakan Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia Jurnal Ternak Tropika Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal JURNAL ILMIAH PETERNAKAN TERPADU ARSHI Veterinary Letters Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat JURNAL KAJIAN VETERINER Jurnal Ilmu Peternakan Terapan Journal of Tropical Animal Science and Technology Agrinimal Jurnal Ilmu Ternak dan Tanaman Wahana Peternakan Jurnal Nukleus Peternakan Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Jurnal Penelitian Inovatif Jurnal Peternakan Lahan Kering Journal of Livestock and Animal Health (JLAH) Animal Agricultura Journal of Innovative and Creativity
Wilmientje Marlene Mesang Nalley
Fakultas Peternakan Universitas Nusa Cendana Kupang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Effect of Fructose Levels in Diluents Citric Acid Egg Yolk on The Quality of Liquid Boar Semen Landrace Mallo, Agustinus; Nalley, Wilmientje Marlene; Bette, Yustiani Yuliana
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 12 No. 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p341-353

Abstract

The aim of the research was to test the addition of fructose (F) in egg yolk citrate diluent (C-EY) on the quality of liquid semen from Landrace pigs during storage. This research used a completely randomized design method consisting of five treatments and five replications. The treatments in question are P0 (0%F), P1 (0.5%F), P2 (1.0%F), P3 (1.5%F) and P4 (2.0%F). The diluted cement is stored at a temperature of 18-20oC. Evaluation of the motility, viability, abnormalities and survival of spermatozoa is carried out every twelve hours. Analysis of variance (ANOVA) of data using SPSS. 25 for windows. The results showed P3 at 12 hours (80.00 ± 0.00%), 24 hours (74.00 ± 2.24%), 36 hours (62.00 ± 2.74%), 48 hours (41.00 ±2.24%) and spermatozoa were able to survive 48 hours of storage. P3 gave the best results (P<0.05) compared to other treatments with motility values of 41.00 ± 2.24%, viability 45.20 ± 1.64%, abnormalities 8.20 ± 1.10% and survival 51.20±3.05 hours. It was concluded that the addition of 1.5% fructose level in the egg yolk citrate diluent could maintain the quality of liquid semen longer than other treatments.
Success Rate Of Artificial Insemination Using Honey In Tris-Egg Yolk Dilution In Peanut Goats Bette, Yustiani Yuliana; Nalley, Wilmientje Marlene
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 1 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i1.p98-108

Abstract

Artificial Insemination (AI) is a technique used to improve the genetic quality and quality of livestock. This study aims to determine the success rate of Artificial Insemination using honey as a Tris-Egg Yolk diluent in peanut goats. Two PE male goats aged 2-3 years as semen sources and 18 peanut goats as acceptor females aged 1-3 years have already given birth and are showing an estrus cycle. Acceptor females were divided into two groups with two replications. group 1, insemination using Tris-KT diluent without honey and Group 2 with Tris-KT+Md diluent 0.75 v/w. The semen used for insemination was liquid semen with a dose of 0.2 ml / head with a concentration of 3.89 ± 0.46 x 109 cells / ml and viability not below 50%. Data were analyzed using the Chi square (X)² test to.determine the effect of treatment on the Non Return Rate (NRR) value parameters for 3 estrus cycles after AI and the Conception Rate (CR) value based on observations of 2 month pregnancy. The results showed that there was no real difference between treatments (P>0.05) in pregnancy rates with NRR and CR values of 77.78% (P1) and 88.88% (P2).
Synergistic Impact of Cholesterol-Loaded Cyclodextrin and Moringa Leaf Extract on Post-Thaw Boar Sperm Kinematics Hine, T. M.; Nalley, W. M.; Uly , K.; Kune , P.; Lawa , A. B.; Setyani , N. M. P.; Bette , Y. Y.; Banamtuan , A. N.; Riwu , R. M. J.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.30

Abstract

Structural and functional damage during cryopreservation usually impairs the quality of boar sperm, especially post-thaw motility and kinematic performance, which restricts its use in artificial insemination programs. Cholesterol-loaded cyclodextrin (CLC) and moringa leaf extract (MLE) are considered possible cryoprotective additives because they are membrane-stabilizing and antioxidants. The study investigated the possible synergistic effect of the Tris-egg yolk (TY) extender in combination with CLC and MLE on the motility and kinematics properties of thawed boar sperm, as identified in a Computer-Assisted Sperm Analysis (CASA) system. Ejaculates of four healthy boars were diluted with Tris-based extender without or with CLC (1 or 2 mg/mL), MLE (1 or 1.5 mg/mL), or both, and frozen with a conventional protocol. Kinematic variables alongside progressive and fast motility were included as post-thaw tests. The findings showed that combination treatment of 2 mg/mL CLC and 1 mg/mL MLE (T7) resulted in a significant increase in all sperm motility and kinematic variables compared to the control group (T0) (p<0.05). In particular, T7 had better progressive motility (38.04%), fast motility (22.42%), and higher kinematic variables, especially VCL (106.34 µm/s), VSL (36.28 µm/s), and VAP (45.41 µm/s), and better displacement and trajectory indices. T7 was always better than the single supplementation and other combinations. Finally, the addition of CLC (2 mg/mL) and MLE (1 mg/mL) into the Tris egg yolk extender could be a successful approach towards optimizing semen cryopreservation in boars.
PENGARUH KOMBINASI FRUKTOSA DAN SUKROSA DALAM PENGENCER TRIS-KUNING TELUR TERHADAP KUALITAS SEMEN CAIR BABI LANDRACE: Influence of Combination of Fructose and Sucrose in Diluent Tris-Yolk Against Quality of Liquid Landrace Boars Semen Samuel Alexsander Singga; Wilmintje Marlene Nalley; Alvrado Bire Lawa; Thomas Mata Hine
Wahana Peternakan Vol. 9 No. 3 (2025): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v9i3.6835

Abstract

The purpose of this study was to examine the effect of the combination of fructose (F) and sucrose (S) in egg yolk Tris-diluent (T-EY) on the quality of Landrace boars semen. This study used an experimental method in a complete randomized design consisting of 5 treatments and 5 repeats so that 25 experimental units were formed. The treatment in question was T0= T-EY; T1= T-EY + F 1.0% + S 0.2%; T2= T-EY + F 1.5% + S 0.2%; T3= T-EY + F 1.0% + S 0.4%; T4= T-EY + F 1.5% + S 0.4%. Good quality semen was diluted with a test diluent, then preserved at a temperature of 18-20o C. The variables studied are motility, viability, abnormalities and survival of spermatozoa. This evaluation is done every 12 hours. The results showed that at 60 hours preservation of T2 treatment resulted in the highest and significantly different spermatozoa motility (P>0.05) with all treatments with spermatozoa motility values (43.00%), spermatozoa viability (43.10%), spermatozoa abnormalities (11.40%), and spermatozoa survival (63.20 hours)). It was concluded that the combination of 1.5% fructose and 0.2% sucrose at T2 in the egg yolk Tris-diluent resulted in the best liquid semen quality in maintaining Landrace pig semen quality during low-temperature storage.     Keywords: Fructose, sucrose, qualitys of semen boars Landrace, Tris-yolk egg  
Level Tepung Daun Kirinyuh (Choromolaena odorata) Terhadap Ukuran Linear Tubuh Dan Rasio Efisiensi Protein Pada Babi Landrace Rut Riwu Ratu; W. Marlene Nalley; Ni Nengah Suryani; Upik Syamsiar Rosnah
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.5308

Abstract

Tujuan penelitian ini adalah mengevaluasi level tepung daun kirinyuh terfermentasi (TDKt) mengurangi penggunaan konsentrat komersial (KK) terhadap ukuran linear tubuh dan rasio efisiensi penggunaan protein babi peranakan landrace. Materi yang digunakan adalah 12 ekor ternak babi peranakan landrace yang berumur 2-3 bulan yang dengan bobot badan awal 7,5-16,5 kg dan rerata 10,87 kg (KV=27,73%). Penelitian ini menggunakan metode percobaan dengan rancangan acak kelompok terdiri dari 4 perlakuan dan 3 ulangan. Perlakuan tersebut adalah B0: ransum tanpa TDKt; B1: ransum + 5% TDKt; B2: ransum + 10% TDKt; B3: ransum + 15% TDKt. Variabel yang diukur adalah ukuran linear tubuh dan rasio efisiensi penggunaan protein. Data yang terkumpul dianalisis menggunakan analisis of variance (Anova) dan apabila berpengaruh dilanjutkan uji beda Duncan. Hasil penelitian ini menunjukan bahwa penggunaan TDKt 5-10% berpengaruh tidak nyata (P>0,05) terhadap ukuran linear tubuh (panjang badan, tinggi badan dan lingkar dada) dan rasio efisiensi penggunaan protein sedangkan penggunaan 15% berpengaruh nyata (P<0,05) terhadap ukuran linear tubuh dan rasio efisiensi penggunaan protein. Kesimpulan dari penelitian ini adalah tepunh daun kirinyuh terfermentasi dapat digunakan hingga 10% dalam ransum untuk mengurangi penggunaan bahan pakan konsemtrat komersial.
Kualitas Semen Beku Babi Peranakan Landrace pada Berbagai Level Madu Dalam Pengencer Tris-Kuning Telur Maria Meldiana Jemamu; Wilmientje Marlene Nalley; Yustiany Yuliana Bette; Thomas Mata Hine
JURNAL KAJIAN VETERINER Vol 14 No 1 (2026): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v14i1.26867

Abstract

Information regarding the optimal concentration of honey in Tris-egg yolk extender to maintain the quality of frozen boar semen remains limited. This study aimed to evaluate the effect of honey supplementation on the quality of frozen semen of landrace crossbred boars. Honey was selected due to its antioxidant properties and its role as an energy source (glucose and fructose), which may protect spermatozoa from cryodamage during the freezing process. This experiment applied a completely randomized design with five treatments and four replications. Semen was collected from four landrace crossbred boar aged -2,5 years with ≥75% motility and ≤20% abnormalities. The treatments were T0 (control without honey), T1 (0,75%), T2 (1,50%), T3 (2,25%), T4 (3%) honey in Tris-egg yolk extender. Observed parameters included motility, viability, abnormality and recovery rate after thawing. Statistical analysis showed that honey supplementation significantly affected (P<0,05) semen quality. The best result was obtained at 2,25% honey (T3),with post-thaw motility of 42,35%, viability of 59,80%, abnormality of 10,11%, and recovery rate of 52,64%. It can be concluded that 2,25% honey supplementation in Tris-egg yolk extender is the optimal concentration for maintaining the quality of frozen semen of landrace crossbred boars.
Pengaruh Tepung Daun Asam Terfermentasi (Tamarindus indica L.) terhadap Kecernaan Serat Kasar dan Lemak Kasar pada Babi Grower Mandanil Arisma Leo; Ni Nengah Suryani; David A. Nguru; W. Marlene Nalley
Jurnal Penelitian Inovatif Vol 6 No 2 (2026): JUPIN Mei 2026
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jupin.2416

Abstract

Tingginya biaya pakan mendorong perlunya bahan baku lokal alternatif, salah satunya tepung daun asam terfermentasi. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaannya terhadap kecernaan serat kasar dan lemak kasar pada babi fase grower. Penelitian menggunakan 12 ekor babi grower (peranakan Landrace × Duroc) dengan bobot awal 30–47 kg. Rancangan acak kelompok (RAK) dengan 4 perlakuan dan 3 ulangan diterapkan. Perlakuan terdiri atas P0 (100% ransum basal), P1 (95% basal + 5% tepung daun asam terfermentasi), P2 (90% basal + 10%), dan P3 (85% basal + 15%). Hasil penelitian menunjukkan bahwa perlakuan tidak berpengaruh nyata (P>0,05) terhadap konsumsi pakan (1,23–1,27 kg/ekor/hari), kecernaan serat kasar (46–49%), maupun kecernaan lemak kasar (62–65%). Dengan demikian, penggunaan tepung daun asam terfermentasi hingga level 15% dalam ransum menghasilkan kecernaan yang setara dengan ransum basal. Penelitian ini mengonfirmasi bahwa tepung daun asam terfermentasi berpotensi sebagai bahan pakan lokal alternatif untuk mendukung formulasi ransum babi yang lebih berkelanjutan.
Pengaruh Level Herbal Terfermentasi dalam Liquid Feed terhadap Ukuran Linier dan Pertumbuhan Relatif Babi Fase Grower David A. Nguru; Yoana D. Fransiska; Ni Nengah Suryani; W. Marlene Nalley
Jurnal Penelitian Inovatif Vol 6 No 2 (2026): JUPIN Mei 2026
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jupin.2419

Abstract

Penelitian ini bertujuan menguji pengaruh penambahan herbal terfermentasi ke dalam pakan cair terhadap ukuran linier tubuh dan laju pertumbuhan babi lokal fase grower (umur 3-4 bulan) bobot awal 34-43 kg dengan rataan 37 kg (KV= 4,12%). 12 ekor babi dibagi dalam Rancangan Acak Lengkap (4 perlakuan, 3 ulangan). Perlakuan: H0 (kontrol), H1 (herbal 100 ml/L air), H2 (150 ml/L), dan H3 (200 ml/L). Parameter yang diamati: panjang badan, tinggi badan, lingkar dada, dan pertumbuhan relatif. Hasil ANOVA menunjukkan pengaruh signifikan (P<0,05). Perlakuan H3 menghasilkan nilai tertinggi pada semua parameter. Disimpulkan bahwa penambahan herbal terfermentasi 200 ml/L air dalam pakan cair memberikan hasil terbaik terhadap ukuran linier tubuh dan pertumbuhan relatif babi fase grower.
PENGARUH LEVEL OPTIMAL EKSTRA DAUN KELOR DALAM TIGA PENGENCER TERHADAP KEBERHASILAN INSEMINASI BUATAN TERNAK BABI SECARA IN VIVO (Effect of optimal Levels Of Moringa Leaf Extract Levels in Three ExtenderOn The Succes Of Artificial Insemination In Pigs... Daniel Kurniawan Tulle Dani Tulle; Wilmientje Marlene Nalley; Thomas Mata Hine; Henderiana Laura Louisa Belli; Arnold Elyazar Manu
JURNAL NUKLEUS PETERNAKAN Vol 13 No 1 (2026): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v13i1.27679

Abstract

Artificial insemination is a modern livestock breeding method that has been widely applied and proven effective in commercial farming systems. This study aimed to evaluate the success of artificial insemination using three types of semen extenders, namely Tris-egg yolk (T-EY), Citrate-egg yolk (C-EY), and Beltsville Thawing Solution-egg yolk (BTS-EY), supplemented with optimal levels of Moringa oleifera leaf extract (MLE), in order to determine the best extender. A total of 18 sows aged 1–2 years were inseminated using liquid semen. A descriptive method was applied to analyze non-return rate (NRR) and conception rate (CR), while a completely randomized design (CRD) was used for service per conception (S/C). The treatments consisted of P1: artificial insemination using T-EY + 2% MLE, P2: C-EY + 4% MLE, and P3: BTS-EY + 2% MLE. The observed variables were NRR, CR, and S/C. The results showed that the T-EY + 2% MLE extender achieved the highest CR and NRR, both reaching 100%, with an S/C value of 1.00. Meanwhile, the C-EY + 4% MLE and BTS-EY + 2% MLE treatments resulted in CR and NRR values of 83.33%, with an S/C value of 1.16. It can be concluded that the T-EY extender supplemented with 2% *Moringa oleifera* leaf extract was the best extender for liquid semen preservation of Landrace boars in artificial insemination programs.
The Effect of Sugarcane Juice (Saccharum officinarum) Substitution in Citrate–Egg Yolk Diluent on the Quality of Frozen Semen of Landrace Boars: Pengaruh Substitusi Sari Tebu (Saccharum Offcinarum) dalam Pengencer Sitrat-Kuning Telur terhadap Kualitas Semen Beku Babi Landrace Paskalis Baylon; Wilmintje Marlene Nalley; Alvrado Bire Lawa; Petrus Kune
Buletin Peternakan Tropis Vol. 7 No. 1 (2026)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.7.1.67-79

Abstract

This study aimed to determine the effect of sugarcane juice substitution in a citrate–egg yolk diluent (CE-Y) on the quality of frozen semen of landrace boars. The semen used in this study was collected from four landrace boars aged between 2–2.5 years using the glove gland method. The study employed a Completely Randomized Design with five treatments and four replications, namely T0 = 100% S-KT + 0% sugarcane juice, T1 = 90% S-KT + 10% sugarcane juice, T2 = 80% S-KT + 20% sugarcane juice, T3 = 70% S-KT + 30% sugarcane juice, and T4 = 60% S-KT + 40% sugarcane juice. All treatments were supplemented with 6% glycerol. Semen was evaluated macroscopically and microscopically. After evaluation, a holding time of 2 hours was applied in S-KT diluent with a ratio of 1:1 at a temperature of 24–26°C. The semen was then centrifuged at 3000 rpm for 15 minutes. The pellet was diluted using a base diluent and then distributed into tubes containing the treatment diluent. The semen was packaged in 0.5 mL straws and equilibrated for 2 hours at 3–5°C. After equilibration, the straws were frozen at a distance of 10 cm above the surface of liquid nitrogen for 10 minutes. The straws were then plunged into liquid nitrogen and stored in a liquid nitrogen container. Thawing was carried out by immersing the frozen semen straws in warm water at 37°C for 30 seconds. After dilution and equilibration, sperm quality was evaluated based on motility, viability, and abnormalities. Post-thaw evaluation included motility, viability, abnormalities, and recovery rate. All obtained data were analyzed using SPSS 27.0. The results showed that sugarcane juice substitution had no significant effect (P>0.05) on motility, viability, abnormalities, and RR. It was concluded that the substitution of sugarcane juice with the addition of citrate–egg yolk diluent did not affect the quality of frozen spermatozoa of landrace boars at the stages of post-dilution, post-equilibration, and post-thawing.
Co-Authors A. Kaka Adyanto Nessy Banamtuan Agus Saputra Agustinus Ridlof Riwu Agustinus Ridlof Riwu Aleksander Kaka Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Anak Agung Gede Sugianthara Arnold Elyazar Manu Arnold Elyazar Manu Arnoldianus Harbin Asael Ebson Nabut B Purwantara B Purwantara B. Purwantara Bambang Purwantara Biqshierz V.W Koelima Burhanuddin * Burhanuddin burhanuddin Daniel Kurniawan Tulle Dani Tulle David Agustinus Nguru Demianus Ferdinand Souhoka Dodu, Tagu Fanny Tesalonika Hilly Faustus Jusing Fian K. Djita Foeh, Nancy D. F. K. Franky M S Telupere Franky M.S. Telupere G Semiadi G. Semiadi Gaina, Cynthia Dewi Gerson Andrimus Naimasus GONO SEMIADI Henderian L. L. Belli Henderiana Belli Henderiana Laura Louisa Belli Hendriana L.L. Belli Herdis . Heri Armadianto Heri Armadianto I Made Adi Sudarma I Made Suaba Aryanta Imanuel Benu Imanuel Benu J A Tamoes Jernih Blegur Johny N. Kihe jourvan manasye marabijala Juliyanti Stefania Salem Kirenius Uly Kirenius Uly kirenius uly Kristina Enjelina Jigha Kune , P. Lawa, Alvrado Bire Lombo, Jelittron Putra Lote, Nyongki Mallo, Agustinus Maman Surachman Maman Surachman Mandanil Arisma Leo Manibak, Maskrim Letlang Mardemus Misa Maria E.E. Fernandez Maria Elisabeth Anjenita Ampur Maria Epifania Barek Maria F. T. Leyn Maria G. Mega Maria Gordince Owa Maria M. Bria Maria Meldiana Jemamu Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Maxs Urias Ebenheizer Sanam Melianus Fafo Michel Johan Matatula MUHAMMAD RIZAL N.G.A Mulyantini S.S Ni Gusti Ayu Mulyantini Ni Made Paramita Setyani Ni Nengah Suryani Ni Nengah Suryani Nuba, Maria Deran Papituan, Mardan Laes Paskalis Baylon Petrus Kune Petrus Kune Petrus Kune Petrus Kune R Handarini R I Arifiantini R. Handarini R. Handarini R. Iis Arifiantini R.I. Arifiantini Reni Ratni Dapawole RI Arifiantini, RI Ristika Handarini Riwu , R. M. J. Riwu, Ronny Markus Jami Rosnah, Upik Syamsiar Rut Listyadi Rut Riwu Ratu Samuel Alexsander Singga Satria J.S. Rihileo Selsiana Dongkot Sepriyono Aklili Setyani , N. M. P. Simon Edison Mulik Sofia Marlize Soleman Manaze Weiri Sinda T L Yusuf T.L. Yusuf Tagu Dodu Tarsisius Considus Tophianong, Tarsisius Considus Theresia Oktaviana Laka Thomas Mata Hine Tree May Thiessen Outang Tuty Laswardi Yusuf Twen O. Dami Dato Ulrikus R. Lole Uly , K. Wirona Adu Yanna Nailla Zulfa Yoana D. Fransiska Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette