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Lukas Pardosi
Prodi Biologi, Universitas Timor, Indonesia
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ISOLASI BAKTERI ASAM LAKTAT DARI FERMENTASI AIR CUCIAN BERAS MERAH DAN UJI ANTIBAKTERI TERHADAP ESCHERICHIA COLI Manalu, Adelya Irawan; Pardosi, Lukas
BIOGENIC : Jurnal Ilmiah Biologi Vol 2 No 2 (2024): BIOGENIC: Jurnal Ilmiah Biologi
Publisher : Program Studi Biologi UNARS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/biogenic.v2i2.5497

Abstract

Bakteri asam laktat (BAL) adalah sekelompok mikroorganisme yang memproduksi asam laktat sebagai produk utama dalam fermentasi karbohidrat. Air bekas pencucian beras merah mengandung karbohidrat yang cukup tinggi, sehingga berpotensi menjadi media yang cocok untuk fermentasi BAL. Tujuan penelitian ini untuk mengisolasi BAL dari fementasi air cucian beras merah dan menguji kemampuannya sebagai agen antibakteri terhadap bakteri patogen. Metode yang digunakan meliputi persiapan sampel, isolasi BAL, pemurnian, karakterisasi isolat, dan uji antibakteri. Isolasi bakteri menghasilkan dua isolat BAL yang menunjukkan karakteristik koloni berbeda dalam bentuk, tepi, elevasi, dan warna. Isolat tersebut adalah bakteri gram positif berbentuk batang dari genus Lactobacillus. Uji antibakteri terhadap E. coli, isolat AC1 menghasilkan zona hambat dengan diameter 8,62 mm, sedangkan isolat AC2 menghasilkan zona hambat sebesar 6,90 mm, bakteri tersebut memiliki potensi sebagai antibakteri.
Pemberdayaan Kelompok Pusat Pengembangan Anak (PPA) Gereja Sion Sasi, Kabupaten Timor Tengah Utara Dalam Mencegah Stunting Melalui Budidaya Jamur Tiram Putih Pardosi, Lukas; Fransiskus Yulius Dhewa Kadju; Adelya Irawan Manalu; Charles Venirius Lisnahan; Wilda Lumban Tobing; Boanerges Putra Sipayung; Desta Gloria Siahaan
MATAPPA: Jurnal Pengabdian Kepada Masyarakat Volume 7 Nomor 3 Tahun 2024
Publisher : STKIP Andi Matappa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31100/matappa.v7i3.3364

Abstract

Universitas Timor melalui program Pemberdayaan Kelompok Masyarakat melakukan kegiatan pengabdian Masyarakat dengan kegiatan  pendampingan budidaya jamur tiram sebagai alternatif pencegahan stunting. Tujuan dari pengabdian ini adalah untuk memberdayakan kelompok Pusat Pengembangan Anak (PPA) Gereja sion sasi dalam melakukan budidaya jamur tiram sebagai alternatif pangan dalam mencegah stunting. Metode yang dilakukan dalam pengabdian ini adalah pelatihan dan edukasi tentang pencegahan stunting dan pemenuhan gizi; pelaksanaan dalam hal ini diberikan pelatihan pembuatan rumah jamur, baglog, penanganan pra panen dan pasca panen; Pengolahan jamur tiram dilakukan dengan melibatkan PPA dalam melakukan pengolahan jamur tiram hasil panen menjadi jamur crispi; Hasil evaluasi dari kegiatan ini menunjukkan bahwa budidaya jamur tiram dan olahannya memberikan dampak yang positif terhadap kelopok PPA. Anak anak yang tergolong stunting sangat menyukai jamur crispi hasil olahan dari jamur tiram. Selain itu budidaya jamur tiram ini juga mempunyai peluang usaha yang dapat dikembangkan dan punya prospek pasar yang baik. Luaran dari kegiatan ini adalah kelompok PPA dapat melakukan budidaya jamur tiram dan pengolahnnya sebagai alternatif pangan bernilai gizi.
Teknologi Pembuatan Pellet Rendah Protein Untuk Budidaya Ayam Kampung Fase Grower Di Kelompok Tani Perempuan Sion Kefamenanu Lisnahan, Charles Venirius; Nahak, Oktovianus Rafael; Pardosi, Lukas
Bakti Cendana Vol 6 No 2 (2023): Bakti Cendana: Jurnal Pengabdian Masyarakat
Publisher : LPPM Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/bc.6.2.2023.129-139

Abstract

Tujuan teknologi pembuatan pakan ayam Kampung dalam bentuk pellet dengan memanfaatkan bahan pakan lokal rendah protein adalah untuk mengefisiensikan biaya pakan sumber protein yang relatif mahal seperti tepung ikan dan bungkil kedelai, dan untuk meningkatkan kesejahteraan Kelompok Tani Perempuan Sion Kefamenanu. Kegiatan ini adalah bagian dari aplikasi program Hibah Penelitian Terapan Berbasis Laboratorium LPPM Unimor tahun 2022. Metode yang digunakan adalah participatory action research, kelompok tani dan tim penelitian bersama menentukan kegiatan di lapangan berdasarkan masalah kemahalan pakan pabrikan (BR1 dan BR2). Solusi dalam mengatasi permasalahan tersebut meliputi kegiatan persiapan bahan dan alat-alat, dan eksekusi di lapangan. Kegiatan dilakukan dengan penyuluhan, pelatihan/pendampingan sekaligus praktek pelaksanaan kegiatan untuk meningkatkan ketrampilan dan kemampuan kelompok. Kegiatan meliputi Pemilihan dan pengadaan bahan pakan lokal yang baik, pengadaan bahan pakan mikro-nutrien seperti asam amino kritis dan vitamin premiks, pencampuran pakan komplit, pembuatan pellet dan penjemuran pellet dan pengepakan. Hasil yang dicapai adalah ketrampilan kelompok tani meningkat dengan memanfaatkan teknik pembuatan pellet dan terjadi efisiensi biaya pakan yang akan digunakan dalam budidaya ayam Kampung. Dari kegiatan ini, kelompok mitra dapat mengefisiensikan biaya pakan sebesar Rp. 3.000/kg dibandingkan dengan pakan yang dibeli dari poultry shop.
PENERAPAN TEKNOLOGI INTERNET OF THINGS (IOT) UNTUK BUDIDAYA JAMUR TIRAM PADA KELOMPOK TANI ANIFU DI DESA FATUNENO, WILAYAH PERBATASAN NKRI-RDTL Pardosi, Lukas
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol. 31 No. 2 (2025): APRIL-JUNI
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkm.v31i2.65537

Abstract

Universitas Timor melalui program Kosabangsa melakukan Kegiatan Pelatihan dan Pendampingan teknologi Internet of Think (IOT) Budidaya Jamur Tiram Pada Kelompok Tani Anifu dalam peningkatan produktivitan, pendapatan dan Ketahanan Pangan kawasan Perbatasan NKRI-RDTL Di Desa Fatuneno Kecamatan Miomaffo Barat Kabupaten Timor Tengah Utara. Pemasalahan prioritas diwilayah ini adalah rendahnya produktivitas pertanian sehingga menjadikan desa fatuneno termasuk wilayah dengan masyarakat yang memiliki pendapatan minim serta kurangnya pelatihan teknologi pertanian seperti budidaya jamur tiram menjadikan desa tersebut rawan pangan. Tujuan dari Kegiatan ini adalah kelompok tani diharapkan menjadi bagian dari percepatan peningkatan ekonomi lokal, ketahanan pangan, dan pertanian berkelanjutan. Metode yang digunakan dalam kegiatan program kosabangsa ini adalah Perticipatory Action Research (PAR). Adapaun tahapan metode Pelaksanaan kegiatan meliputi: 1) Sosisalisi pengenalan budidaya jamur. 2) Pelatihan pembuatan kumbung jamur, pembuatan media baglog jamur tiram, sterisilisasi media baglog, inokulasi bibit. 3) Evalusi Kegiatan dilakukan dengan cara pendampingan kegiatan pra panen dan pasca panen untuk keberlanjutan program. Selain itu kegiatan dievaluasi dengan instrumen berupa kuisioner dan dianalisis secara deskriptif. Hasil Pengabdian yang didapatkan dari kegiatan ini adalah menunjukkan kelompok tani anifu mampu melakukan budidaya jamur tiram putih serta mendapatkan keterampilan dalam pengembangan budidaya jamur tiram. Kata Kunci: Budidaya Jamur, Kelompok tani Anifu, Ketahanan Pangan.
Pelatihan Pembuatan Preparat Basah dan Kering Tumbuhan Berbasis Kearifan Lokal di SMAS Pelita Karya: Training in Making Wet and Dry Plant Preparations Based on Local Wisdom at SMAS Pelita Karya Kamaluddin, Kamaluddin; Siahaan, Desta Gloria; Manalu, Adelya Irawan; Pardosi, Lukas
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 6 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i6.8880

Abstract

This community service aims to provide training to teachers and students of SMAS Pelita Karya in making wet and dry plant preparations based on local wisdom. This activity was motivated by the lack of laboratory facilities and the limited ability of teachers to carry out effective biology practicums. The training was carried out using the In-Service Training (IST) and On-Service Training (OST) methods involving science teachers and students. The results of this activity showed an increase in the competence of teachers and students in making and utilizing wet and dry preparations from local plants for biology practicum activities. In addition, there was an increase in students' understanding of biological concepts through the use of contextual and relevant preparation media to their surroundings. This activity concludes that training in making preparations based on local wisdom can improve the quality of biology learning at SMAS Pelita Karya and enrich learning methods based on local resources.
Isolation of Chitinolytic Bacteria from Shrimp Waste as a Control of Aedes aegypti Instar III Mosquito Larvae Pardosi, Lukas
Biodidaktika : Jurnal Biologi dan Pembelajarannya Vol 20, No 2 (2025)
Publisher : Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/biodidaktika.v20i2.25041

Abstract

Insect-borne diseases remain a serious health problem in Indonesia, especially Dengue Hemorrhagic Fever (DHF), which can cause mass mortality in developing countries. Biological control, such as using chitinolytic bacteria, is needed to contain the spread of this disease. The methods used include sampling and preparation of shrimp waste, manufacture of chitin agar media, isolation of chitinolytic bacteria, macroscopic and biochemical characterization, maintenance of Aedes aegypti larvae, observation of effects on mosquito larvae, and data analysis. The results obtained there are six isolates that have different morphological characteristics: IS1 to IS6 isolates have a round shape and milky white color, with wavy edges on IS1, IS2, and IS4, and flattened on other isolates. At elevation, IS1 and IS5 are flat, while the other four isolates are convex. Microscopic tests found 2 isolates contained Gram-negative and 4 isolates Gram-positive. The biochemical test of all six isolates was positive. Effect of administration of the concentration that caused the highest time of death of larvae on the fourth day, with a concentration of 15% and an average number of deaths of 4.66. Based on the results of the study, it can be concluded that six isolates of chitinolytic bacteria were successfully isolated, with diverse morphological characteristics, including differences in shape and color. Microscopic tests identified two isolates as Gram-negative and four isolates as Gram-positive. The biochemical test results showed that the six isolates were positive in the catalase, Simmons Citrate Agar (SCA), and Triple Sugar Iron Agar (TSIA) tests. Concentration administration affected the time of larval death, with a concentration of 15% showing the highest mortality rate on the fourth day, with an average death toll of 4.66
Isolation and Identification of Bacteria from Cattle Rumen Fluid and Their Application on In Vitro Digestibility of Rice Straw Bira, Gerson Frans; Sio, Stefanus; Batu, Matius S; Pardosi, Lukas; Kia, Kristoforus W; kolo, Maria Magdalena; Nubatonis, Alfred
Journal of Tropical Animal Science and Technology Vol. 7 No. 3 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to isolate and identify bacteria from the rumen fluid of Bali cattle and to evaluate their effect as microbial inoculants on the in vitro digestibility of rice straw. The experiment was conducted using a Completely Randomized Design (CRD) consisting of four treatments and four replications. The treatments included rice straw silage with the addition of 0%, 5%, 10%, and 15% of local microorganisms (MOL) derived from cattle rumen fluid. The observed variables included the isolation and identification of bacteria from the rumen fluid, and the assessment of in vitro digestibility, including Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), Volatile Fatty Acid (VFA) concentration, and ammonia (NH₃) production. The results showed that the addition of rumen fluid MOL significantly (P<0.05) improved the digestibility parameters. The highest DMD (52.44%) and OMD (47.11%) were obtained from the treatment with 15% MOL. VFA and NH₃ concentrations also increased, indicating enhanced microbial activity and fermentation efficiency. The study concludes that MOL derived from cattle rumen fluid, particularly those containing Bacillus sp., is effective in improving the nutritional quality and digestibility of rice straw
Education of making and distribution of betel leaves hand sanitizers to kefamenanu Pasar Baru sellers Mada, Grandianus Seda; Tea, Marselina Theresia Djue; Pardosi, Lukas; Welsiliana; Pramita, Dira Asri; Batu, Matius Stefanus; Siahaan, Desta Gloria
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 2 (2020): ABDIMAS TALENTA : Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1050.895 KB) | DOI: 10.32734/abdimastalenta.v5i2.4307

Abstract

The business’ level, lack of knowledge about the making process and use of hand sanitizers from natural ingredients caused sellers in Pasar Baru Kefamenanu do not apply the health protocols during transactions with buyers. Therefore, there needs to be educated about natural ingredients that can be used as hand sanitizers, the making process, and how to use them. Natural ingredients that are found in Kefamenanu are betel leaves. Education on making hand sanitizers from betel leaves helps sellers to be able to make their own hand sanitizers. The addition of aloe vera to betel leaves extract aims to make the liquid hand sanitizer becomes softer. While lime and perfume are to get rid of the betel leaves’ smell. The distribution of hand sanitizers that has been made can be an example of products from natural ingredients and the storage bottles provided can be used as a refill place for hand sanitizer.
Pemberdayaan Kelompok Tani Kana Melalui Pelatihan Budidaya Jamur Tiram Berbasis Pemanfaatan Limbah Furniture Manalu, Adelya Irawan; Pardosi, Lukas; Tobing, Wilda Lumban; Lisnahan, Charles Venirius
Jurnal Pengabdian UNDIKMA Vol. 5 No. 2 (2024): May
Publisher : LPPM Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jpu.v5i2.10702

Abstract

This community service activity aims to increase the knowledge and skills of the Kana farmer group in cultivating oyster mushrooms by utilizing furniture waste. The method of implementing this service uses participatory action research with the stages of planning, implementation, monitoring, and evaluation. The evaluation instrument for this activity uses a questionnaire sheet, and then the data obtained is analyzed descriptively. The results of this service show that there is an increase in knowledge about the utilization of furniture waste in oyster mushroom growth media and provide skill experience to farmer groups in cultivating oyster mushrooms.
SOSIALISASI DAN OPTIMALISASI E-LEARNING BAHASA INGGRIS DAN BIOLOGI MENGGUNAKAN EDMODO KEPADA MAHASISWA PRODI PETERNAKAN UNIMOR Siahaan, Desta Gloria; Pardosi, Lukas
Dharma Pengabdian Perguruan Tinggi (DEPATI) Vol 4 No 1 (2024): Mei 2024
Publisher : Fakultas Sains dan Teknik, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/depati.v4i1.4557

Abstract

One of the e-learning platforms that can be utilized during the Covid-19 pandemic is Edmodo. Edmodo learning media which is very functional, besides being able to be used for students and lecturers, can also be used by parents. Assignments were given through the assignment and quiz features that have a time limit, so they can practice discipline in doing assignments. The objectives of implementing this activity are; First, providing the concept and socialization of the use of Edmodo for the learning process. Second, providing knowledge on how to use and apply the features contained in the Edmodo platform to facilitate the English learning process. The target of this service was Animal Husbandry II C students who in this semester are studying English and Biology. This community service was able to provide knowledge to use the Edmodo e-learning platform for free to support conventional learning during this covid-19 pandemic. This activity lasted for 1 day in one of the room of the Faperta Unimor building.
Co-Authors Agustina Yovita Boluk Alfred Nubatonis Anastasia Buak Anggelina Mokos Anjelina M. da Cruz Arkianti, Maria Maxmilla Yoche Azor Yulianus Tefa Banusu, Maria Sarliana Batu, Matius S Benu, Didi Prasetyo Bira, Gerson Frans Bria, Deseriana Charles Venirius Lisnahan Dira Asri Pramita Eduardus Yosef Neonbeni Elmalinda Moensaku Emanuel M. Y. Hano’e Emanuel M. Y. Hano’e Emilia Juliyanti Bria Faesal, Andi Fallo, Gergonius Florian Mayesti Prima Remba Makin Fobia, Kaprisius G. Fransiskus Yulius Dhewa Kadju Hanas, Dicky Frengky Hano’e, Emanuel M. Y. Hermina Manlea Hermina Manlea I Gede Arya Wiguna Jefry Presson Jefry Presson jefry Presson Jendri Mamangkey Josua Sahala Jusanty Antoneta Pakh Kamaluddin Kamaluddin Kamaluddin Kamaluddin Kia, Kristoforus W Kia, Kristoforus Wilson Kia, Kristoforus. W. Kolo, Maria Magdalena Korbafo, Elisabeth Kristoforus Wilson Kia Lidya, Christie Liem, Noviana Animra Lisnahan, Charles V. Lusiana Yeni Akoit Mada, Grandianus Seda Manalu, Adelya I Mardit N Nalle Maria Ernalinda Seran Matius Stefanus Batu Meta Permata Putri Metriana Leon Moensaku, Elmalinda Nahak, Oktovianus Rafael Naimuni, Fransiskus Xaverius Natalia Desy Djata Ndua Natalia Hoar Neonbeni, Eduardus Yosef Nugraha K. F. Dethan Pakh, Jusanty Antoneta Pramita, Dira Asri Pramusita, Santa Maya Presson, Jefry Respati Tri Swasono Riska Septia Wahyuningtyas Risna Erni Yati Adu Sabirin Matsjeh Sefrinus Maria Dolfi Kolo Seuk, Mariana Septiani Siahaan, Desta Gloria Sine, Yuni Sio Stefanus, Sio Sipayung, Boanerges Putra SITUMORANG, KOMILIE Tae, Lidwina Felisima Tahulending, Peggy Tea, Marselina Theresia Djue Tefa, Anna tnunay, ite morina yostianti Tode, Sonya Yuniarsi Welsiliana Welsiliana Widhya Faustina Wiguna, Gede Arya Wilda Lumban Tobing Yakobus Pffeferius Edvend Saba Agu Yohana Fatmawati Usfinit Yos Bobi A. Olla Yuliana Kolo Yuni Sine Yuni Sine Zulfah Az Zahra