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Journal : Indonesian Journal of Biotechnology

The synergistic effect of doxorubicin and ethanolic extracts of Caesalpinia sappan L. wood and Ficus septica Burm. f. leaves on viability, cell cycle progression, and apoptosis induction of MCF­7 cells Sari Haryanti; Suwijiyo Pramono; Retno Murwanti; Edy Meiyanto
Indonesian Journal of Biotechnology Vol 21, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1290.778 KB) | DOI: 10.22146/ijbiotech.26105

Abstract

Caesalpinia sappan L. and Ficus septica Burm. f  known asa potential plant with wide variety of medicinal properties, including anticancer. Present study was aimed to explore cytotoxic effect ofsappan wood (ECS) and awar-awar leaves (EFS), and its combination with doxorubicin (dox) on MCF-7 cells focusing on cell cycle progression and apoptosis induction.The result of MTT assay showed that single treatment of ECS and dox performed cytotoxic effect with the IC50 value of 32 µg/mL and 6 µM respectively, while EFS performed low cytotoxic effect with the IC50 value of 282 µg/mL. The combination of ECS with EFS and doxorubicin showed synergistic cytotoxic effect. Flow cytometry analysis revealed that combination of ECS (16 µg/mL) with EFS (8 µg/mL) and doxorubicin (2 µM) induced apoptosis, and cell accumulation at sub-G1 and G2/M phases.Immunoblotting assay confirmed the apoptosis induction of this combination through increasing of cleavage of PARP-1. Based on these results, the synergistic cytotoxic effect of this combinationwas through G2/M phase accumulation and apoptosis inductionand potentially to be developed as co-chemotherapeutic agent.
Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection Widiastuti, Dyah; Paramita, Dewi Kartikawati; Murwanti, Retno; Kusrini, Ina; Wijayanti, Nastiti; Haryanto, Aris
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111102

Abstract

Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen was developed by cloning the LipL32 gene conserved portion of Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi. The immunoinformatic was used to characterize the developed rLipL32. Western blot results using anti‐histidine revealed a band of rLipL32 protein at ~40 kDa. Subsequently, it was used to examine the IgM antibody on human sera by using ELISA. The IgM‐LipL32 ELISA was evaluated using 67‐positive and 25‐negative sera and compared with a commercial ELISA. With a cut‐off value of 0.8, it showed 85.7% sensitivity, 83.3% specificity, a 48% positive prediction value (PPV), and 97% negative prediction value (NPV), indicating modest performance compared to existing commercial kits. The rLipL32 is a potential antigen for detecting IgM using ELISA; however, for use in low incidence areas, a confirmation test is crucial.
Co-Authors Abdul Rohman Adam Hermawan Agung Endro Nugroho Agung Endro Nugroho Ahmad Marzuki Alexxander, . Alexxander, . Amalia, Latifa Amilia Amilia, Amilia Anami Riastri Andayana Puspitasari Gani Andayana Puspitasari Gani Andayana Puspitasari Gani Annisa, Siti Nur Anselma Ivanawati Arief Nurrochmad Arief Nurrochmad Arief Nurrochmad Arief Nurrochmad Arief Rahman Hakim Arifka, Vigha Ilmanafi Aris Haryanto Ariska Deffy Anggarany, Ariska Deffy Ashfannada, Nuqya Asri Mega Putri Azizah Amin Azizah, Ulfah Laily Azmi Rahmadani Bambang Sulistiyo Ari Sudarmanto Bani Adlina Shabrina Bayu Irawan, Muhamad Bayu Prio Septiantoro Beni Lestari Budastra, Wayan Cintya Ganes Cahyo Nurani, Sekar Cindy Puspita Sari Devina Martina Devita Anggraeni Devita Anggraeni Devyanto Hadi Triutomo Dewi Kartikawati Paramita Dhania Novitasari Dhirgo Adji Dhirgo Adji Diana Rachma Ningsih Dwi Brilyani Sandy Dwi Salim, Rozin Dyah Afritasari Dyah Aryani Perwitasari Dyah Widiastuti Dyaningtyas Dewi Pamungkas Putri Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Effendi, Adha Maula Erna Prawita Setyowati Erna Prawita Setyowati Fikriansyah Fikriansyah Fita Rahmawati Fitri, Dafina Aisya Fitriana Hayyu Arifah Gagas Pradani Nur Ilmawati Gani, Andayana Puspitasari Ginna Zabrina Hayati, Farida Hendri Wasito Herwandhani Putri Hidayah, Alifia Brilliani Hilyatul Fadliyah I Gusti Ngurah Agung Dewantara Putra Ida Ayu Putu Sri Widnyani Idlohatud Dilalah Ika Puspita Sari Illian, Didi Nurhadi Imono Argo Donatus, Imono Argo Ina Kusrini Iren Wati Siahaan Jenie, Riris Istighfari Juang Juansa Khafi, Muhammad Khasanah, Aisyah Nur Khoerul Anwar Kustanto, Satya Prima Layung Sekar Sih Wikanthi Ledi, Ledi Klarismawati Lodyta Nawang Tika lubis, muhammad fauzan Lukman Hakim Luthfiyyah, Annisa Tiara Maharani, Astrid Martien , Ronny Mentari Widiastuti Miftahus Sa'adah Muharom, Luthfi Ali Mustofa Mustofa Naisbitt Iman Hanif, Naisbitt Iman Nanang Fakhrudin, Nanang Nanda Saifullah Sulaiman, Teuku Nastiti Wijayanti Ngurah Agung Dewantara Putra, I Gusti Nindi Wulandari Nindya Budiana Putri Nindya Budiana Putri Ningsih, Diana Rachma Norita Citra Yuliarti, Norita Citra Novrizal Abdi Sahid , Muhammad Nunung Yuniarti Nursani, Rabila Nurul Mukhlisa Pratiwi, Rima Dwi Prawitasari, Saptya Prisnu Tirtanirmala Probowulan, Diyah Purnomo, Kurnia Rahayu Purwantiningsih Purwantiningsih Rahmawaty Rachmady Rahmayani, Almira Retno S. Sudibyo Retno Sunarminingsih Sudibyo Riandari , Teti Mariam Rima Munawaroh Risa Umari Yuli Aliviyanti Ritmaleni, Ritmaleni Rizkita, Anggraini Ihza Rohmad Yudi Utomo Rohman, Abdul Roissinta, Agnesca Rommy Sagala, Reynelda Juliani Sardjiman . Sari Haryanti Sari Haryanti Sari, Laras Ratna Shofa Annur Siswadi Siswadi Sri Susilowati Sri Tasminatun Sri Wahdaningsih Subagus Wahyuono Sudarsono Sudarsono Sugeng Riyanto Sugiyanto . Sugiyanto . Sulaiman, T.N Saifullah Sumaiyah, Sumaiyah Susi Ari Kristina Suwijiyo Pramono Suwijiyo Pramono Tamhid, Hady Anshory Tampubolon, Keshia Thoriq Ziyad, Thoriq Tiffany, Ghea Rachella Triana Candraningrum Triana Hertiani Triawan Adi Cahyanto Ulfa Afrinurfadhilah Darojati Wahyu Utaminingrum Widya Leontin Susanti Wulandari, Febri Yance Anas Yoce Aprianto Yosi Bayu Murti Yundari, Yundari Zahrotul Ulum Ziana Walidah, Ziana