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Evaluating autologous peritoneum grafting for enhanced healing of bile duct injuries: A preliminary data from an animal study Nugroho, Anung N.; Mudigdo, Ambar; Soetrisno, Soetrisno; Yarso, Kristanto Y.; Nurwati, Ida; Indarto, Dono; Pamungkasari, Eti P.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1873

Abstract

Increased incidence of laparoscopic cholecystectomy-related bile duct injuries (BDIs), combined with its risk of serious complications and mortality, highlights the need for a more effective repair technique. Although the use of autologous graft in BDI repair has been promoted, the role of autologous parietal peritoneum remains underexplored. The aim of this study was to evaluate the effect of autologous parietal peritoneum grafts in rabbit models of partial BDI, emphasizing its effect on the expression of cluster of differentiation 68 (CD68) and transforming growth factor-β (TGF-β). An experimental post-test-only design was employed, using 27 male New Zealand rabbits (Oryctolagus cuniculus) aged 8–10 months. The rabbits were allocated into three groups: control (primary closure), autologous parietal peritoneum graft, and autologous gallbladder graft. Partial BDI measuring 15×5 mm were surgically created and repaired according to group assignments. The expression of CD68 and TGF-β were measured via enzyme-linked immunosorbent assay (ELISA), while the anastomosis was pathologically examined through hematoxylin and eosin (H&E) staining on days 3, 7, and 14 post-surgery. Statistical analysis was performed using analysis of variance (ANOVA) followed by Bonferroni post hoc tests. No statistically significant difference was observed in the expression of CD68 or TGF-β among the three treatment groups on days 3, 7, and 14 post-surgery, indicating that the effects of autologous parietal peritoneum graft were comparable to the control and the autologous gallbladder graft in promoting wound healing. Fibroblast density on day 3 was significantly lower in the parietal peritoneum group (p=0.040), reflecting delayed recruitment, but normalized by day 14, indicating successful integration and remodeling. The study highlights the potential role of autologous parietal peritoneum grafts for BDI.
Evaluasi Respon Pendidikan Seksualitas Komprehensif Berbasis Sekolah Pada Siswa SMP Negeri Di Kabupaten Sragen Utami, Dyah Rahmawatie Ratna Budi; Nurwati, Ida; Lestari, Anik
JURNAL PENDIDIKAN KESEHATAN Vol. 5 No. 1 (2025): Jurnal Pendidikan Kesehatan
Publisher : LPPM Sekolah Tinggi Ilmu Kesehatan Pekanbaru Medical Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64931/jks.v5i1.125

Abstract

Kurangnya integrasi pendidikan seksual dengan kurikulum pada remaja menjadikan perlunya implementasi pendidikan seksual komprehensif berbasis sekolah. Penelitian ini bertujuan untuk melakukan evaluasi respon atas pelaksanaan pendidikan seksualitas komprehensif yang diselenggarakan di SMP Negeri Kabupaten Sragen. Penelitian dilakukan dengan metode kualitatif menggunakan pendekatan fenomenologi. Pengumpulan data menggunakan wawancara mendalam. Hasil penelitian menunjukkan pendidikan seksualitas komprehensif memberi dampak positif siswa dalam memahami dan menguatkan sikap serta memudahkan guru dalam mengajarkan materi kesehatan seksual reproduksi. Kesimpulan: Pendidikan seksualitas komprehensif efektif dalam meningkatkan pencegahan perilaku seksual berisiko bagi siswa dengan respon positif dari siswa maupun guru.
The Role of Interleukin 1β, Fibroblast Growth Factor, Fibroblasts, Keratinocytes, Granulation Tissue and Collagen Density in the Wound Healing Phase (Study of Wound Healing in the Inflammation, Proliferation, and Remodeling Phases) Christanto, Antonius; Cilmiaty, Risya; Setiamika, Made; Yudhani, Ratih Dewi; Dirgahayu, Paramasari; Pamungkasari, Eti Poncorini; Nurwati, Ida
Biocaster : Jurnal Kajian Biologi Vol. 5 No. 3 (2025): July
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v5i3.399

Abstract

This scoping review explores the roles of interleukin 1β, fibroblast growth factor, fibroblasts, keratinocytes, granulation tissue, and collagen density in the wound healing process, focusing on inflammation, proliferation, and remodeling phases. A systematic literature search identified studies investigating molecular and cellular mechanisms involved in wound repair. Findings reveal that fibroblast-derived exosomes carrying miR-93-5p inhibit autophagy, delaying diabetic wound healing. Controlled growth factor delivery enhances angiogenesis, fibroblast proliferation, and collagen deposition, accelerating tissue regeneration. SPRR1B+ keratinocytes facilitate rapid re-epithelialization, while granulation tissue provides essential scaffolding for cell migration and neovascularization. Elevated IL-1β impairs healing by increasing matrix metalloproteinases, degrading collagen. Natural compounds like red fruit oil and Binahong leaf extract promote angiogenesis and collagen synthesis. Genetic variations in inflammatory cytokines influence healing outcomes, indicating potential for personalized therapies. This review consolidates current evidence, providing insights into cellular and molecular interactions critical for effective wound repair and guiding future regenerative medicine strategies.
The Analysis of Strawberry Powder Content and Potential Implications as Anti-Obesity Fadhilah, Azzahra Nur; Nurwati, Ida; Rahardjo, Setyo Sri
Indonesian Journal of Global Health Research Vol 7 No 3 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i3.6125

Abstract

The prevalence of obesity in Indonesia has increased from 21.8% in 2018 to 23.4% in 2023. Contributing factors include an unbalanced diet, lack of physical activity, and genetic factors. One strategy for dealing with obesity is regulating diet with nutritious intake. Strawberries are rich in antioxidants and flavonoids that are beneficial for obese groups, such as improving lipid profiles and reducing oxidative stress. Its high water content makes is perishable, processing it into powder can increase shelf life without reducing its nutritional content. This article examines the nutritional content and benefits of strawberry powder in managing obesity. Method: A descriptive research design with laboratory analysis approach. The powder making process began with drying using a cabinet dryer at a temperature of ±40-600C for 16 hours, followed by grinding process using a grinder, the compound content in strawberry powder was analyzed. Analysis revealed that 100 grams of strawberry powder contained 3.18% fat, 6.43% protein, 71.67% carbohydrates, 0.57% crude fiber, 397.72 mg vitamin C, 77.63% antioxidant inhibition and 555.10 ppm anthocyanin. The drying and powdering processes didn’t significantly reduce the compound content of the strawberry. Strawberry powder demonstrated potential as an anti-obesity agent, offering benefits in improving obesity biomarkers.
Combination of Metformin and Magnesium Citrate Reduces TNF-α, NF-κB p65, IL-6, CD4, and MMP-9 Expressions in Diabetic Model Rats Rahayu, Rachmi Fauziah; Prayitno, Adi; Purwanto, Bambang; Soewondo, Widiastuti; Nurwati, Ida; Pamungkasari, Eti Poncorini; Dirgahayu, Paramasari
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3360

Abstract

BACKGROUND: Diabetes, which causes various complications, involves pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), nuclear factor kappa B p65 (NF-κB p65), interleukin-6 (IL-6), cluster of differentiation 4 (CD4), and matrix metalloproteinase-9 (MMP-9). Magnesium has demonstrated anti-diabetic properties, but its anti-inflammatory effects in preventing cardiovascular complications remain unclear. This study aimed to evaluate the anti-inflammatory effects of magnesium citrate, alone and in combination with metformin, by measuring TNF-α, NF-κB p65, IL-6, CD4, and MMP-9 expression in diabetic model rats.METHODS: Thirty male Wistar rats were divided into five groups: normal control, diabetes control, metformin (treated with 9 mg/200 g/day metformin), magnesium citrate (treated with 3.6 mg/200 g/day magnesium citrate), and combination therapy (treated with 4.5 mg/200 g/day metformin + 1.8 mg/200 g/day magnesium citrate). Diabetes was induced in all groups except the normal control group using streptozotocin (STZ) and nicotinamide (NA). TNF-α, NF-κB p65, IL-6, CD4, and MMP-9 expression levels were measured using enzyme-linked immunosorbent assay (ELISA).RESULTS: Significant differences in TNF-α, NF-κB p65, IL-6, CD4, and MMP-9 expression levels were observed across all groups (p<0.001). The combination therapy group demonstrated the most significant reduction in all parameters compared to the diabetic control group (p<0.001) and other therapy groups. Both metformin and magnesium citrate monotherapies showed moderate reductions in cytokine levels but were less effective than combination therapy.CONCLUSION: Combination therapy with metformin and magnesium citrate exhibited the most potent anti-inflammatory effects, significantly reducing TNF-α, NF-κB p65, IL-6, CD4, and MMP-9 expressions in diabetic Wistar rats. This combination has potential as a therapeutic approach for managing diabetes and its complications.KEYWORDS: diabetes mellitus, inflammation, cytokines, metformin, magnesium citrate
The Effect of Durio Zibethinus L. Seed Extract on Fasting Blood Glucose and Insulin Resistance in Metabolic Syndrome Model Rats Aisyah, Siti; Harioputro, Dhani Redhono; Nurwati, Ida
Jurnal Kesehatan Manarang Vol 10 No 3 (2024): Bahasa Inggris
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v10i3.1526

Abstract

Metabolic syndrome is known as insulin resistance syndrome, characterized by chronic hyperglycemia. Management of metabolic syndrome involves several combinations, including lifestyle modifications and pharmacological interventions. Durian seeds are one source of antioxidants that have the potential to improve blood glucose homeostasis and insulin sensitivity. This study aims to analyze the effect of durian seed extract on changes in fasting blood glucose levels and insulin resistance. The extraction process utilized the maceration method with a 70% ethanol solution. This study consisted of 30 rats divided into six treatment groups: KN (normal group), K- (negative control), K+ (positive control metformin 9 mg/kgBW), P1, P2, and P3, which were given durian seed extract at doses of 100 mg/kgBW, 200 mg/kgBW, and 300 mg/kgBW, respectively. Metabolic syndrome rats were induced with a high-fat, high-fructose diet for 14 days, then induced with streptozocin (STZ) and nicotinamide (NA). Fasting blood glucose levels were determined using the GOD-PAP method. The HOMA-IR index was used to measure insulin resistance. The data results were evaluated using a paired T-test and one-way ANOVA. The analysis showed a significant variance in fasting blood glucose and HOMA-IR index following a 21-day treatment (p<0.05). The highest decrease was found in the 300 mg/kgBW dose group with fasting blood glucose and HOMA-IR index of 90.00 ± 2.70 mg/dl and 3.55 ± 0.11. The P2 and P3 treatments did not show different results with metformin treatment (p>0.05). The findings of this study suggest that consumption of durian seed extract for 21 days can effectively improve the condition of mice with metabolic syndrome. In addition, the drug metformin has the same effect as the intervention of durian seed extract doses of 200 and 300 mg/kgBW.
Comparison of Lipid Profiles among Vegan Vegetarians, Lacto-Ovo Vegetarians and Non-Vegetarians Sineri, Dessy Ikasartika; Nurwati, Ida; Sumardiyono, Sumardiyono
JURNAL INFO KESEHATAN Vol 22 No 4 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss4.1741

Abstract

The lipid profile is a crucial indicator of cardiovascular disease risk, with elevated levels often influenced by dietary patterns. Nutrient intake and physical activity can help regulate cholesterol by reducing total cholesterol, HDL, LDL and triglyceride levels. This study aims to compare the lipid profiles among vegan vegetarians, lacto-ovo vegetarians, and non-vegetarians. This research uses a cross-sectional study design. The study population includes 60 participants from IVS and the Yogyakarta community, selected based on inclusion and exclusion criteria. The research examines diet type as the independent variable, lipid profile as the dependent variable, and physical activity as a confounding variable. Data collection involves respondent identity and a physical activity questionnaire using IPAQ. Statistical analyses include one-way ANOVA, Post Hoc tests and multivariate analysis with one-way MANCOVA. The research results show that the total cholesterol level for vegan (173.10±21.04), lacto-ovo (169.45±44.84), and non-vegetarians (194.55±31.40). HDL levels for vegan are (55.25±10.79), lacto-ovo (49.00±9.12), and non-vegetarians (45.49±9.11). LDL levels for vegan are (111.90±19.80), lacto-ovo (114.85±44.39), and non-vegetarians (134.90±30.37), showing no significant difference p>0.05. Triglyceride levels for vegan are (103.15±57.06), lacto-ovo (100.65±60.07), and non-vegetarians (154.15±88.63) with significant differences p-value <0.05. When controlling for physical activity, differences in total cholesterol, HDL, and triglycerides persist between diet groups. Notably, HDL levels differ significantly between vegans and lacto-ovo (p<0.05). The conclusion is that both vegan and lacto-ovo diets have lipid profiles within the normal range compared to non-vegetarian diets. A vegetarian diet and regular physical activity contribute to a healthier lipid profile.
Studi Potensi Anti-Fertilitas Ekstrak Etanolik Akar Kelor (Moringa oleifera, Lam) Melalui Tingkat Ekspresi Caspase-3 Testis Tikus Wistar (Rattus norvegicus) Model Sindrom Metabolik Terinduksi Budiani, Dyah Ratna; Nurwati, Ida; Subandono, Jarot; Hermawan, Danus; Mahendra, Fadia Yuda
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i4.58173

Abstract

Penelitian Penelitian ini bertujuan untuk mempelajari apoptosis sel germinal pada ductus seminiferous dan pengaruh dosis ekstrak etanolik akar kelor terhadap spermatogenesis pada sindrom metabolik. Enzim caspase-3, yang berperan sebagai eksekutor utama apoptosis, digunakan untuk mengukur tingkat apoptosis yang terjadi. Apoptosis sel germinal yang tinggi pada ductus seminiferous menunjukkan peningkatan infertilitas. Penelitian eksperimental laboratorik ini menggunakan 30 tikus Wistar model sindrom metabolik yang diinduksi dengan diet tinggi lemak dan STZ-Na, serta 6 ekor kontrol normal. Kelompok perlakuan menerima ekstrak etanolik akar kelor dengan dosis berbeda (150, 250, dan 350 mg/kgBB/hari). Hasil penelitian menunjukkan penurunan ekspresi caspase-3 pada kelompok dengan dosis 150 mg/kgBB/hari (K3), sedangkan dosis 250 mg/kgBB/hari (K4) dan 350 mg/kgBB/hari (K5) meningkatkan ekspresi caspase-3. Selain itu, terjadi peningkatan kerusakan sel germinal dan penurunan spermatogenesis pada kelompok sindrom metabolik yang diberi ekstrak akar kelor, yang tercermin pada penurunan nilai Johnsen score. Peningkatan caspase-3 dan penurunan skor Johnsen menunjukkan peningkatan apoptosis dan penurunan spermatogenesis, yang berpotensi meningkatkan infertilitas.
DURIAN SEED EXTRACT AMELIORATES LIPID PROFILES IN METABOLIC SYNDROME MODEL RATS Rusliana, Eka; Nurwati, Ida; Ardyanto, Tonang Dwi
Media Penelitian dan Pengembangan Kesehatan Vol. 35 No. 1 (2025): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v35i1.2393

Abstract

Sindrom metabolik merupakan sekumpulan kelainan metabolik yang kompleks dan berkaitan dengan terjadinya dislipidemia yang ditandai dengan adanya perubahan profil lipid yang abnormal meliputi peningkatan kadar trigliserida, kadar low-density lipoprotein (LDL) dan rendahnya kadar high-density lipoprotein (HDL). Ekstrak biji durian mengandung senyawa flavonoid, alkaloid, fenolik dan triterpenoid yang berpotensi dalam memperbaiki profil lipid. Penelitian ini bertujuan untuk mengamati ekstrak biji durian dalam perbaikan profil lipid. Penelitian ini merupakan eksperimental laboratorium desain pretest-posttest dengan kelompok kontrol. Sebanyak 30 ekor tikus jantan galur Wistar usia 8 minggu, berat 150-200 g dibagi menjadi 6 kelompok. Kelompok kontrol normal (NG) yaitu tikus yang diberi pakan standar, sedangkan 5 kelompok ainnya dibuat model sindrom metabolik dengan diberi diet High Fat High Fructose (HFHFr) 14 hari dan induksi  Streptozotocin (STZ)-Nicotinamide (NA), meliputi kelompok kontrol negatif (NC), yang diberi aquades, dan kelompok kontrol positif (PC), yang diberi simvastatin 0,9 mg/kgBB. Kelompok perlakuan yaitu kelompok perlakuan 1 (TG1), kelompok perlakuan 2 (TG2), dan kelompo perlakuan 3 (TG3), diberikan ekstrak biji durian dosis 100, 200, dan 300 mg/kgBB selama 21 hari. Data dianalisis menggunakan Paired T-test dan One-way ANOVA, dengan signifikansi p<0,05. Hasil menunjukkan setelah 21 hari pemberian ekstrak biji durian terjadi penurunan kadar trigliserida dan LDL serta peningkatan kadar HDL yang signifikan (p<0,05), yang mana perubahan terbesar terjadi pada kelompok dosis 300 mg/kgBB dengan trigliserida sebesar -38,24 ± 6,45 mg/dl, LDL -45,67 ± 2,71 mg/dl dan HDL 54,22 ± 2,72 mg/dl. Pemberian ekstrak biji durian dapat memperbaiki profil lipid pada sindrom metabolik dengan dosis terbaik yaitu 300 mg/kgBB.
STUDI KUALITATIF KETIDAKNYAMAN MUSKULOSKELETAL PADA IBU HAMIL TRIMESTER TIGA Wuriani, Wuriani; Nurwati, Ida
Jurnal Review Pendidikan dan Pengajaran Vol. 7 No. 3 (2024): Volume 7 No 3 Tahun 2024
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v7i3.30562

Abstract

Kehamilan dengan nyeri punggung bawah adalah keluhan umum yang terjadi pada wanita hamil. Nyeri punggung yang dirasakan sangat erat hubungannya dengan posisi ibu pada masa kehamilan khususnya posisi tidur dan mekanika tubuh selama hamil. Kehamilan trimester tiga merupakan titik puncak munculnya keluhan sakit muskuloskeletal, hal ini dikarenakan beban pada perut ibu semakin berat dan tulang punggung dan kaki ibu berusaha keras untuk menopang tubuhnya. Tujuan penelitian Mengeksplorasi ketidaknyamanan khususnya pada sistem muskuloskeletal yang dialami ibu hamil trimester tiga di wilayah kerja Puskesmas Rasau Kaya, Kubu Raya Metode penelitian yang dilakukan adalah kualitatif dengan melakukan wawancara mendalam pada dua orang ibu hamil trimester tiga terkait keluhan muskuloskeletal atau Musculoskeletal disorders yang dirasakan, yang terkait postur gerakan dan ketidaknyamanan yang dialami. Hasil dari penelitian didapat hasil ada empat domain besar yang dapat diangkat pada penelitian ini, yaitu 1) lokasi dan karakteristik ketidaknyamanan yang dirasakan ibu hamil trimester tiga., 2) faktor penyebab ketidaknyamanan, 3) tentang cara mengatasi ketidaknyamanan sistem muskuloskeletal pada ibu hamil trimester tiga. Kesimpulan dari penelitian ibu hamil trimester tiga mengalami penambahan berat badan sangat signifikan, maka akan menjadi tumpuan beban tubuh adalah tulang dan otot , sehingga akan timbul beberapa ketidaknyamanan pada tulang dan otot, dan akan ada cara untuk mengurangi keluhan tersebut.
Co-Authors A. Guntur Hermawan Adi Prayitno Ambar Mudigdo Anik Lestari Asrial, An A. Baihaqy, Fathu Thaariq Bambang Purwanto Betty Saptiwi Betty Saptiwi Betty Saptiwi Brian Wasita Christanto, Antonius Danus Hermawan Danus Hermawan Dicky Maulana Lazuardi Diding H P Diding Heri Prasetyo Diding Heri Prasetyo Dono Indarto Dyah Rahmawatie Ratna Budi Utami Dyah Ratna Budiani Dyah Ratna Budiani Dyah Ratna Budiani Eriana Melinawati Eric Edwin Yuliantara Eti Poncorini Pamungkasari Fadhilah, Azzahra Nur Fahmi, Muhammad Izzul Widad Gresmita Rindi Winarti Hadi, Cahyono Harioputro, Dhani Redhono Hermawan, Danus Hermawan, Danus Ika Tristanti Indri Erwhani Jarot Subandono Jarot Subandono Jarot Subandono Khoirunnisa, Rahmi Lazuardi, Dicky Maulana Mahendra, Fadia Yuda Martini Martini Martini Martini Martini Martini Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Mutmainah Mutmainah Nabila Aushaf Prasetyo Nafisa, Putri Amalina Nanang Wiyono Noni Kartika Sari Nugroho, Anung N. Nur Hafidha Hikmayani Nur Hafidha Hikmayani Nutria Widya Purna Anggraini Octavia, Dhamayanti Eka Pamungkasari, Eti P. Paramasari Dirgahayu Prihandjojo Andri Putranto Pudjiastuti, Anggit Putranto, R. Prihandjojo Andri Putranto, Wachid Rachmi Fauziah Rahayu Rahardjo, Setyo Sri Ratnasari Ratnasari Reviono Reviono Risya Cilmiaty A. R. Risya Cilmiaty AR Risya Cilmiaty, Risya Rosyida, Rufaida Rusliana, Eka Sari, Noni Kartika Sarsanti, Arifa Sherina Noor Sarsono Sarsono Sarsono Sarsono Sarsono Sarsono Sarsono Sarsono Selfi Handayani Selfi Handayani Selfi Handayani Selfi Handayani Setiamika, Made Setianto, Budi Y. Sigit Setyawan Sineri, Dessy Ikasartika Siti Aisyah Siti Marufah Soetrisno Soetrisno Soewondo, Widiastuti Sri Hartati Hadinoto Sri Haryati Sri Sulistyowati Sumardiyono Sumardiyono Sumardiyono, S Tonang Dwi Ardyanto Uki Retno Budihastuti Vitri Widyaningsih Wahyu Anjas Sari Wesliaprilius, Todung Antony Wiyono, Nanang Wuriani, Wuriani Yarso, Kristanto Y. yoseph Indrayanto Yudhani, Ratih Dewi Yulia Sari Yulyanti, Yulyanti