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Priyono Priyono
Department Of Physics, Faculty Of Sciences And Mathematics, Diponegoro University
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Journal : BERITA BIOLOGI

 1 
PERBANYAKAN TANAMAN BUAH NAGA BERDAGING BUAH MERAH {Hylocereus costaricensis) MELALUI TEKNIK KULTUR JARINGAN Priyono, Priyono
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.379 KB) | DOI: 10.14203/beritabiologi.v7i5.878

Abstract

Hylocereus costaricensis is new important fruit in Indonesia. One of constrain in its development is limitation of planting material.The aim of the research is to study the regeneration H. costaricensis through micro shoot induction of node explants. The experiments were carried out in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute. Experiment on microshoots proliferation stage was arranged in a Factorial Completely Randomized Design, with three replications.The first factor was Kinetin concentration consisted of five treatments i.e.: 0, 2.5, 5.0, 7.5 and 10.0 mg/1.The second factor was Indole acetic acid (IAA) concentration consisted of five levels i.e.: 0, 0.25, 0.5, 0.75 and 1 mg/1. Microshoots multiplication stage was arranged in a Factorial Completely Randomized Design,with three replications. The first factor was polyvinyl pyrrolidone (PVP) concentration consisted of six treatments i.e.: 0, 0.25, 0.5, 0.75, 1.0 and 1.25 %. The second factor was Cystein concentration consisted of four treatments i.e.: 0, 25, 50 and 75 mg/1. The microshoots rooting stage the results experiment was laid in a Factorial Completely Randomized Design, with three replications. The first factor was Giberalic acid (GA3) consisted of five treatments i.e.:0, 0.25, 0.5, 0.75 and 1 mg/1. The second factor was Boric acid concentration consisted of four levels i.e.: 0, 50, 100 and 150 mg/1. In the microshoots proliferation stage the results showed that there was interaction between IAA and Kinetin concentration on the microshoots proliferation and the number of shoot per explnat. The best results were obtained from the treatment 0.75 mg/1 IAA + 7.5 mg/1 Kinetin, whereas in this treatment the rate of microshoots proliferation and the number of microshoots perexplant was 50 % and 3.9, respectively. In the microshoots multiplication stage, the results showed that there was interaction between PVP and Cystein concentration. The best results were obtained from the treatment 0.75% PVP + 75 mg/1 Cystein, whereas in this treatment the rate of microshoots multiplication and the number of microshoots per explant was 95% and 6.3, respectively. In the rooting stage, the results showed that there was interaction between GA and Boric acid concentration. The experiment indicated that 0.5 mg/1 GA3 + 100 mg/1 Boric acid showed the best result to stimulate root induction of the in vitro microshoots propagation, whereas in this treatment the percentage of rooted microshoots and the hight of plantlet were 95% and 5.7 Cm, respectively.
REGENERASI TANAMAN KERKLILY {Lilium longiflorum Thunb.) MELALUI EMBRIOGENESIS SOMATIK PADA EKSPLAN DAUN Priyono, Priyono
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (700.97 KB) | DOI: 10.14203/beritabiologi.v5i4.1125

Abstract

The effects of plant growth regulators on somatic embryo genesis in Lilium longiflorum Thunb.leaf cultures were studied.The aim of the research is to study the regeneration ofi. longiflorum via somatic embryogenesis of leaf explant.IAAat5 level of concentrations (0-1 mg/1) combined with BAP at 4 level of concentrations (0-2 mg/1) were tested to induction of somatic embryogenesis in the first stage. Combination of two cytokinins, namely BAP and kinetin, combined with either of four auxins, namely IAA, IBA, NAA or 2,4D were tested to induce the somatic embryo genesis in the second stage. Combination of two concentrations of ABA, namely 0 and 1 mg/1 combined with 5 level concentrations of GA3, namely 0, 0.25, 0.5, 0.75 and 1 mg/1 were tested to improve the somatic embryos maturation.The results showed that somatic embryos were promoted by addition of IAA and BAP. The study of auxin and cytokinin combinations on somatic embryogenesis showed that somatic embryogenesis of kerk lily induced by addition of auxin and cytokinin in the culture medium.The maximum somatic embryo induction was obtained on medium contained of 0.25 mg/1 IAA combined with 1 mg/1 kinetin. Somatic embryos maturation were improved by transferring the somatic embryo from induction medium to maturation medium that contained 0.5 mg/I GA3 +1 mg/1 ABA.
PENGARUH ARAH DAN UKURAN POTONGAN SISIK UMBI KERK LILY (Lilium longiflorum Thunb.) TERHADAP PEMBENTUKAN TUNAS MIKRO DAN BULBLET SECARA IN VITRO Priyono, Priyono; Winarsih, Sri
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.634 KB) | DOI: 10.14203/beritabiologi.v5i1.1102

Abstract

Mass production by using micro propagation technique has an important role for Lilium longiflorum regarding that it can produce seedlings in relatively short time, disease free, and regularly time. Several factors affected the successful micro shoots and bulblets formation, i.e.medium, explant, and temperature. This research aimed to know the effect of direction and width of scale section on the multiplication of kerk lily in vitro.Research has been conducted in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute, Jember.The research was arranged in factorial completely randomised design, i.e.direction of scale section and width of scale section. Two levels of direction of scale section namely longitudinal and transversal and five levels of width of scale section namely 1, 4, 6, 8, and 10 mm.The results showed that scales are able to produce micro shoot only, bulblet only, or both micro shoot and bulblet.The number of micro shoots produced from longitudinal section are higher than those of produced from transversal section. Width of scale section affected micro shoots and bulblets production, which their correlations are quadratic.The trend of micro shoots production was contrary with bulblet production.
Co-Authors A Manaf A. Haris A. Rusgiyono A.A. Ketut Agung Cahyawan W Abidin, Nur Achmad Imam Agung AF Kurniawan, AF Agfianto, Tomi Agung Cahyono agung Pramana Agus Anggoro Sigit Agus Subagio Agus Trihandoyo Agus Ulinuha Ahmad Asrori Ahmad Sulaiman, Ahmad Alfina, Alfina Ali Haidir Alif Noor Anna Aminuyati Anas M Najih Andi Andi Andi Gunawan Andi Saryoko Andoyo, Andreas Andrianto, Sukrisna Andriayani, Novita Angelina, Dian Anggrelanggi, Arsy Anggrellanggi, Arsy Arif Jauhari Arisandy, Poppy Arisanty, Poppy ARIWIBOWO, PRASETIO Arnelli Arnelli Artian, Artian Sirun Artian Aryo Tunjung Kusumo Asahi, Adam Aslinda, Aslinda Astia Ningsih, Dwi Atien Priyanti Atok, Laurensius Kristo Augustine, Yvonne Auliani, Faulhia B. Raharjo Bawano, Franklin Bawono, Ari Bianome, Wofrid Bianome, Wofrid Erlista Bianome, Wolfrid Brilliant, Muhamad Choirul Amin Choirul Anam AM Diponegoro D. Ispriyanti Darwin Rio Budi Syaka David David Dian Ekowati Dian Puspita Dian Puspita Sari Didik Setiyo Widodo Dilahur Dilahur, Dilahur Dima, Adid Balle Dina Syafrianingsih, Dina Dwi Astuti Dwi Budi Santoso Dwi Purwantoro Sasongko Dwi Wahyu Nugroho Dwiantoro, Tino E. Setiawati Edy Suprapto Endang Sri Sudalmi Eris, Deden Dewantara Erma Kumala Sari Etty Murwaningsari Evi Gusliana F. Ariyanto Fadila, Hana Fadrul, Fadrul Fahira, Talitha Khansa Fahrizal Fahrizal Fahrizal Fahrizal Fajar Arianto Fajar Arif Mustofa Fatur, Tiyo Fauziyah, Ridha Feta, Neneng Rachmalia Fiqhan Muslim , Ibnu fiqhan muslim, ibnu Fitri, Septi Bunal Fitria Fitria Fitriyah, Fauziatul Fransisca, Luciana guntoro, adi H. Sutanto H. Sutanto Henny Armaniah Henny Destiana Heri Sutanto Hindriari, Reni Hocky, Agus Hodo, Yeremias Lake Hudzaifah Hazazi Zia Kusuma Hutahuruk, Marice Br I Gusti Bagus Wiksuana I Gusti Putu Asto Buditjahjanto I Nurhasanah I. Marhaendrajaya Ihwan Susilo, Ihwan Iis Nurhasanah Ilmy Zaada Faidullah Imam Hardjono Indras Marhaendrajaya Ishak Yassir Isti’ana Maftuchah, Isti’ana J. E. Suseno Jasman Jasman Jatmiko Endro Suseno Jeprianto, Jeprianto Joko Sudarto Joni Joni Jumadi Jumadi Juwanda, Farikhin K Sofjan Firdausi K, Basri K. S. Firdausi, K. Karnaji Karnaji Karnaji, Karnaji Karyono Karyono Ketut Sofjan Firdausi, Ketut Sofjan Kharis Triyono Khasan Rowi Kholifah, Desiana Nur Kilat Permana Putra Kliwon Hidayat Komardi, Dadi Kristanto, Daniel Kudri, Muhammad Wan Kudri, Wan Muhammad Kukuh Dwi Septiana Kusuma, Annisa Dian Kusumo, Aryo Tunjung Kuswaji Dwi Priyono Laban, Banjar Yulianto Laia, Maria Monika Septa Lengo, Edwinurius Berlinus Leni Anggraeni Leotman, Baradista Dimas Lila Dini Utami Lilik Anifah Liu, Mikhael Dendy Robertus Luktyansyah, Insyiah Meida M. Imam Taufiq M. Izzati M. Nur M. Sholahuddin Manesi, Damianus Manurung, Lengsi Mau, Sealtial Mau, Sealtiel Maximilian M. J. Kapa, Maximilian M. J. Mega Lestari Mnune, Edwin Deriston Muh Yasin YBIC Muhammad Abdul Aziz Muhammad Adhimawan Wijaya Muhammad Faisal Muhammad Farid Aminudin, Muhammad Farid Muhammad Hilman Fakhriza, Muhammad Hilman Muhammad Rivai Mujiono Mujiono Mukti, Nursanti Anjune Munawar Cholil Muryanto Nanda, Adi Prasetia Nasution, Ali Napiah Nila Elfrida Harahap Nofiar, Nofiar Nofrizal Nofrizal Novita Andriyani Nufus, Khayatun Nurdin Ibrahim Nurhasanah, Iis Ariska Nurkholis Nurkholis Nurlaila Nurlaila Nurul Aisyah Nurul Taufiqu Rochman Nurwenda Novan Maulana Nyoto Nyoto, Nyoto Panca Saputra, Elin Pandji Triadyaksa Paramita, Safira Arta Pardoyo Pardoyo Parulian, Dudi Passu, Ahas Weros Peginusa, Stefani Switly PRAKOSO, Haryo Tejo Putra, Tri Mandala Putri, Ega Harnanditya Putri, Novia Kelana Putri, Novita Yulia R. Hariyati R. Hastuti Radyum Ikono Rahayu, Adi RAHMAD MAHENDRA SARWIJIWO Rahmat Tri Yunandar Ramadhani, Syaikhah Aditya Ranam, Sanudin Ravik Karsidi Regina Ayu Andini Rezki, Muhammad Ricky Ricky Rike Yudianti Rike Yudianti Rina Wati Rismaya, Siska Riyaji, Akbar Rizaldi Putra Rizki Restu Setiyani Rizki, Fahlul Rohana, Krisma Dwi Rosihan Asmara RR. Ella Evrita Hestiandari Rudiansyah, Firman Rudiyanto Rudiyanto, Rudiyanto S Siswanto S, Yvonne Augustin S. Suryono S. Tana Saiful Bahri Sanggam Ramantisan Saputara, Elin Panca Saputra, Yose Saputro, Achmat Yulyadi Saputro, Avisema Sigit Sartono Joko Santosa Satria, Rita Siregar, Valdi Muhamad Rafiansyah Siregar, Valdi Muhammad Rafiansyah Sirih, Muhammad Sistya Rachmawati Siswadi Siswadi Sitepu, Bina Swasta Siti Mukodimah Soekarno Mismana Putra, Soekarno Mismana Sri Hartati Sri Iswiyanti, Agus Sri Wahyuni Subagya Subagya, Subagya Subagyo Subagyo Subhan Subhan Sudarno Sudarno Sugito Sugito Suhardjono Suhardjono Suharja, Iman Suhartono Suhartono Sulastri Sulastri Suldalmi, Endang Sri Sulistyawan A Sulistyo Sulistyo Sunardi Sunardi Sunarhadi, M. Amin Sundari Utami, Shinta Supit, Teve W. M. Supratiwi, Mahardika Suryadhitia, Rachmat Suryadithia, Rachmat Susanto, Yohanes Karoberd Yugi Suswandi, Suswandi Suyono Suyono Syah Putra, Arman Syukur, Adnan T. Windarti Tage, Oktafianus Tamelan, Paul G. Taufik Rahman Taufik Taufik Teguh Teguh Thaka, Indra Tito Prastyo Rahman Tnunay, Imanuel To, Yonas Tri Atmoko Tri Susilowati U Kaltsum, U Ulfa Isni Kurnia Ummi Kaltsum Umrotun Umrotun Valencia, Erica Very Richardina Very Richardina Vincensius Gunawan S.K. W. H. Rahmanto Wahyu Agustina, Wahyu Wahyudin Wahyudin Wibisono, Sigit A. Wibisono, Sigit A. Wibisono, Yusub Widiyanto Widiyanto Widyastuti, Lina Ayu Windu Ganar Prasongko Windu Ganar Prasongko Wofrid E. Bianome Wulandari Wulandari Yeni Susanti Yulanda, Novidya Yuli Priyana Yuswono Yuswono Z. Muhlisin Zaenul Muhlisin