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Priyono Priyono
Department Of Physics, Faculty Of Sciences And Mathematics, Diponegoro University
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Journal : BERITA BIOLOGI

 1 
PERBANYAKAN TANAMAN BUAH NAGA BERDAGING BUAH MERAH {Hylocereus costaricensis) MELALUI TEKNIK KULTUR JARINGAN Priyono, Priyono
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.379 KB) | DOI: 10.14203/beritabiologi.v7i5.878

Abstract

Hylocereus costaricensis is new important fruit in Indonesia. One of constrain in its development is limitation of planting material.The aim of the research is to study the regeneration H. costaricensis through micro shoot induction of node explants. The experiments were carried out in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute. Experiment on microshoots proliferation stage was arranged in a Factorial Completely Randomized Design, with three replications.The first factor was Kinetin concentration consisted of five treatments i.e.: 0, 2.5, 5.0, 7.5 and 10.0 mg/1.The second factor was Indole acetic acid (IAA) concentration consisted of five levels i.e.: 0, 0.25, 0.5, 0.75 and 1 mg/1. Microshoots multiplication stage was arranged in a Factorial Completely Randomized Design,with three replications. The first factor was polyvinyl pyrrolidone (PVP) concentration consisted of six treatments i.e.: 0, 0.25, 0.5, 0.75, 1.0 and 1.25 %. The second factor was Cystein concentration consisted of four treatments i.e.: 0, 25, 50 and 75 mg/1. The microshoots rooting stage the results experiment was laid in a Factorial Completely Randomized Design, with three replications. The first factor was Giberalic acid (GA3) consisted of five treatments i.e.:0, 0.25, 0.5, 0.75 and 1 mg/1. The second factor was Boric acid concentration consisted of four levels i.e.: 0, 50, 100 and 150 mg/1. In the microshoots proliferation stage the results showed that there was interaction between IAA and Kinetin concentration on the microshoots proliferation and the number of shoot per explnat. The best results were obtained from the treatment 0.75 mg/1 IAA + 7.5 mg/1 Kinetin, whereas in this treatment the rate of microshoots proliferation and the number of microshoots perexplant was 50 % and 3.9, respectively. In the microshoots multiplication stage, the results showed that there was interaction between PVP and Cystein concentration. The best results were obtained from the treatment 0.75% PVP + 75 mg/1 Cystein, whereas in this treatment the rate of microshoots multiplication and the number of microshoots per explant was 95% and 6.3, respectively. In the rooting stage, the results showed that there was interaction between GA and Boric acid concentration. The experiment indicated that 0.5 mg/1 GA3 + 100 mg/1 Boric acid showed the best result to stimulate root induction of the in vitro microshoots propagation, whereas in this treatment the percentage of rooted microshoots and the hight of plantlet were 95% and 5.7 Cm, respectively.
REGENERASI TANAMAN KERKLILY {Lilium longiflorum Thunb.) MELALUI EMBRIOGENESIS SOMATIK PADA EKSPLAN DAUN Priyono, Priyono
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (700.97 KB) | DOI: 10.14203/beritabiologi.v5i4.1125

Abstract

The effects of plant growth regulators on somatic embryo genesis in Lilium longiflorum Thunb.leaf cultures were studied.The aim of the research is to study the regeneration ofi. longiflorum via somatic embryogenesis of leaf explant.IAAat5 level of concentrations (0-1 mg/1) combined with BAP at 4 level of concentrations (0-2 mg/1) were tested to induction of somatic embryogenesis in the first stage. Combination of two cytokinins, namely BAP and kinetin, combined with either of four auxins, namely IAA, IBA, NAA or 2,4D were tested to induce the somatic embryo genesis in the second stage. Combination of two concentrations of ABA, namely 0 and 1 mg/1 combined with 5 level concentrations of GA3, namely 0, 0.25, 0.5, 0.75 and 1 mg/1 were tested to improve the somatic embryos maturation.The results showed that somatic embryos were promoted by addition of IAA and BAP. The study of auxin and cytokinin combinations on somatic embryogenesis showed that somatic embryogenesis of kerk lily induced by addition of auxin and cytokinin in the culture medium.The maximum somatic embryo induction was obtained on medium contained of 0.25 mg/1 IAA combined with 1 mg/1 kinetin. Somatic embryos maturation were improved by transferring the somatic embryo from induction medium to maturation medium that contained 0.5 mg/I GA3 +1 mg/1 ABA.
PENGARUH ARAH DAN UKURAN POTONGAN SISIK UMBI KERK LILY (Lilium longiflorum Thunb.) TERHADAP PEMBENTUKAN TUNAS MIKRO DAN BULBLET SECARA IN VITRO Priyono, Priyono; Winarsih, Sri
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.634 KB) | DOI: 10.14203/beritabiologi.v5i1.1102

Abstract

Mass production by using micro propagation technique has an important role for Lilium longiflorum regarding that it can produce seedlings in relatively short time, disease free, and regularly time. Several factors affected the successful micro shoots and bulblets formation, i.e.medium, explant, and temperature. This research aimed to know the effect of direction and width of scale section on the multiplication of kerk lily in vitro.Research has been conducted in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute, Jember.The research was arranged in factorial completely randomised design, i.e.direction of scale section and width of scale section. Two levels of direction of scale section namely longitudinal and transversal and five levels of width of scale section namely 1, 4, 6, 8, and 10 mm.The results showed that scales are able to produce micro shoot only, bulblet only, or both micro shoot and bulblet.The number of micro shoots produced from longitudinal section are higher than those of produced from transversal section. Width of scale section affected micro shoots and bulblets production, which their correlations are quadratic.The trend of micro shoots production was contrary with bulblet production.
Co-Authors A Manaf A. Haris A. Rusgiyono A.A. Ketut Agung Cahyawan W Abidin, Nur Achmad Imam Agung AF Kurniawan, AF Agfianto, Tomi Agung Cahyono Agus Anggoro Sigit Agus Subagio Agus Trihandoyo Agus Ulinuha Ahmad Asrori Ali Haidir Alif Noor Anna Aminuyati Anas M Najih Andi Andi Andi Gunawan Andi Saryoko Andoyo, Andreas Angelina, Dian Anggrellanggi, Arsy Arif Jauhari Arisandy, Poppy Arisanty, Poppy ARIWIBOWO, PRASETIO Arnelli Arnelli Artian, Artian Sirun Artian Aryo Tunjung Kusumo Astia Ningsih, Dwi Atien Priyanti Augustine, Yvonne Auliani, Faulhia B. Raharjo Bahri, Saiful Bawano, Franklin Bawono, Ari Bianome, Wofrid Erlista Bianome, Wolfrid Choirul Amin Choirul Anam AM Diponegoro D. Ispriyanti David David Dian Puspita Didik Setiyo Widodo Dilahur Dilahur, Dilahur Dima, Adid Balle Dina Syafrianingsih, Dina Dwi Budi Santoso Dwi Purwantoro Sasongko Dwi Wahyu Nugroho Dwiantoro, Tino E. Setiawati Edy Suprapto Endang Sri Sudalmi Eris, Deden Dewantara Erma Kumala Sari F. Ariyanto Fadila, Hana Fahira, Talitha Khansa Fahrizal Fahrizal Fajar Arif Mustofa Fatur, Tiyo Fauziyah, Ridha Feta, Neneng Rachmalia Fiqhan Muslim , Ibnu fiqhan muslim, ibnu Fitria Fitria Fitriyah, Fauziatul Fransisca, Luciana guntoro, adi H. Sutanto H. Sutanto Henny Armaniah Henny Destiana Heri Sutanto Hindriari, Reni Hocky, Agus Hodo, Yeremias Lake Hudzaifah Hazazi Zia Kusuma Hutahuruk, Marice Br I Gusti Bagus Wiksuana I Gusti Putu Asto Buditjahjanto I Nurhasanah I. Marhaendrajaya Ihwan Susilo, Ihwan Iis Nurhasanah Ilmy Zaada Faidullah Imam Hardjono Indras Marhaendrajaya Isti’ana Maftuchah, Isti’ana J. E. Suseno Jasman Jasman Jatmiko Endro Suseno Joko Sudarto Joni Joni Juwanda, Farikhin K Sofjan Firdausi K, Basri K. S. Firdausi, K. Karnaji Karnaji Karyono Karyono Ketut Sofjan Firdausi, Ketut Sofjan Kharis Triyono Khasan Rowi Kholifah, Desiana Nur Kilat Permana Putra Kliwon Hidayat Kristanto, Daniel Kudri, Muhammad Wan Kudri, Wan Muhammad Kukuh Dwi Septiana Kusumo, Aryo Tunjung Kuswaji Dwi Priyono Laia, Maria Monika Septa Lila Dini Utami Lilik Anifah Luktyansyah, Insyiah Meida M. Imam Taufiq M. Izzati M. Nur Manesi, Damianus Manurung, Lengsi Mau, Sealtial Mau, Sealtiel Maximilian M. J. Kapa, Maximilian M. J. Muh Yasin YBIC Muhammad Abdul Aziz Muhammad Faisal Muhammad Farid Aminudin, Muhammad Farid Muhammad Hilman Fakhriza, Muhammad Hilman Muhammad Rivai Mujiono Mujiono Munawar Cholil Muryanto Nanda, Adi Prasetia Nasution, Ali Napiah Nofiar, Nofiar Nofrizal Nofrizal Nufus, Khayatun Nurdin Ibrahim Nurkholis Nurkholis Nurul Taufiqu Rochman Nurwenda Novan Maulana Nyoto Nyoto, Nyoto Panca Saputra, Elin Paramita, Safira Arta Pardoyo Pardoyo Passu, Ahas Weros PRAKOSO, Haryo Tejo Putri, Ega Harnanditya Putri, Novia Kelana Putri, Novita Yulia R. Hariyati R. Hastuti Radyum Ikono Rahayu, Adi RAHMAD MAHENDRA SARWIJIWO Rahmat Tri Yunandar Ramadhani, Syaikhah Aditya Ranam, Sanudin Regina Ayu Andini Rezki, Muhammad Ricky Ricky Rike Yudianti Rike Yudianti Rismaya, Siska Riyaji, Akbar Rizaldi Putra Rohana, Krisma Dwi Rosihan Asmara RR. Ella Evrita Hestiandari S Siswanto S, Yvonne Augustin S. Suryono S. Tana Sanggam Ramantisan Saputara, Elin Panca Saputro, Achmat Yulyadi Saputro, Avisema Sigit Sartono Joko Santosa Satria, Rita Siregar, Valdi Muhamad Rafiansyah Siregar, Valdi Muhammad Rafiansyah Sirih, Muhammad Siswadi Siswadi Soekarno Mismana Putra, Soekarno Mismana Sri Hartati Sri Wahyuni Subagya Subagya, Subagya Subhan Subhan Sudarno Sudarno Sugito Sugito Suhardjono Suhardjono Suhartono Suhartono Sulastri Sulastri Suldalmi, Endang Sri Sulistyawan A Sulistyo Sulistyo Sunarhadi, M. Amin Sundari Utami, Shinta Supratiwi, Mahardika Suryadhitia, Rachmat Suryadithia, Rachmat Suswandi, Suswandi Suyono Suyono Syukur, Adnan T. Windarti Tage, Oktafianus Tamelan, Paul G. Taufik Rahman Thaka, Indra Tito Prastyo Rahman To, Yonas Tri Atmoko Tri Susilowati U Kaltsum, U Ummi Kaltsum Valencia, Erica Very Richardina Vincensius Gunawan S.K. W. H. Rahmanto Wibisono, Sigit A. Widiyanto Widiyanto Widyastuti, Lina Ayu Windu Ganar Prasongko Windu Ganar Prasongko Wofrid E. Bianome Yulanda, Novidya Yuli Priyana Yuswono Yuswono Z. Muhlisin Zaenul Muhlisin