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Sukenda .

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680

Author-ID : 390256

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Meningkatkan kemampuan pemahaman siswa dengan menggunakan metoda penemuan terbimbing pada pembelajaran geometri kelas 8 Sukenda Sukenda; Uba Umbara; Evan Farhan Wahyu Puadi
JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan Vol 4 No 1 (2018): Edisi Vol. 4 No. 1 Mei
Publisher : Program Studi Pendidikan Matematika STKIP Muhammadiyah Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.231 KB)

The purpose of the study was to investigate whether Guided Finding Methods could improve students' understanding of geometry in class 8. The method used is Classroom Action Research. The research was done at SMP Negeri 2 Ciwaru located at Jl. Raya Sumberjaya, Desa Citikur, Kecamatan Ciwaru, Kabupaten Kuningan. The subjects of the researchs were the students of SMP Negeri 2 Ciwaru class 8 academic year 2016/2017. They were chosen because after consulting with mathematics teachers that this had major problems regarding the comprehension ability of mathematical concepts. Data collection techniques: observation, interview and questionnaire. The research was done in 2 cycles, each cycle was done in 3 sessions. Each session includes 4 stages: planning, action, observation and reflection. The results of the research as follows: Learning with guided discovery method can improve students' understanding 73.82%. With the following details on the first cycle students who reached the KKM 17 students (62.54%) with an average score of 65.74. In cycle II the number of KKM 23 students (85.19%) the average score in cycle II was 79.26. Based on the results of the research it is advisable to use guided discovery methods in improving students' comprehension skills.

Analisis Hukum Perjudian Togel di Wilayah Hukum Polres Indramayu: Perspektif Hukum Positif Sukenda, Sukenda
Strata Law Review Vol. 2 No. 1 (2024): March
Publisher : CV. Strata Persada Academia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59631/slr.v2i1.184

Efforts to combat gambling crimes can be carried out in practice through preemptive measures, widespread public awareness campaigns, preventive actions (before the occurrence of any gambling crime), and repressive actions to enforce the law against individuals engaged in gambling activities. These three prevention efforts are interdependent and form an inseparable chain in combating criminal activities in general, including specifically Togel gambling crimes. How does the Indramayu Police Resort undertake efforts to prevent Togel gambling crimes from a positive legal perspective? In this research, the author employs a normative method. In legal studies, the normative method refers to an approach that focuses on determining what "should be" law or legal policy based on moral criteria or widely recognized values. Law enforcement efforts to prevent and eradicate gambling crimes, especially Togel gambling, are necessary to prevent its proliferation and maintain social harmony. Law enforcement is the process of implementing legal desires for society to obey and is an obligation to do so. The goal of law enforcement is to enhance societal order and legal certainty. This is achieved by regulating institutions tasked with enforcement according to their respective scopes and through effective cooperation contributing to goal achievement.

Peer-Assisted Bulk-Synchronous Content Distribution Service: Sistem Cerdas Pendukung Akreditasi Perguruan Tinggi IAPS 4.0 Berbasis Nextcloud dan Python Laksana, Eka Angga; Sukenda
Jurnal Ilmiah Komputasi Vol. 23 No. 3 (2024): Jurnal Ilmiah Komputasi : Vol. 23 No 3, September 2024
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32409/jikstik.23.3.3611

Peer-assisted Bulk-Synchronous Content Distribution Service merupakan sebuah aplikasi cerdas yang dibangun untuk mendukung proses akreditasi program studi perguruan tinggi. Aplikasi ini dibuat tidak untuk menggantikan system ataupun kebiasaan-kebiasaan yang telah ada namun lebih kepada melengkapi atau bahkan meningkatkan proses yang telah terjadi. Ada dua komponen fundamental Peer-assisted Bulk-Synchronous Content Distribution Service yaitu dokumen borang akreditasi dan Cloud Repository. Keduanya akan disatukan dalam sebuah service cerdas yang mampu men-sinkronkan kalimat di dalam dokumen borang dengan file-file bukti pendukungnya di dalam Cloud Repository. Hal ini terinspirasi dari proses akreditasi perguruan tinggi yang selalu melibatkan bukti-bukti pendukung terkait dengan kalimat-kalimat atau data yang tertulis di dokumen. Penelitian ini dikembangkan dengan memanfaatkan Bahasa pemrograman Python dan Nextcloud API. Python sebagai alat untuk membangun otomasi beberapa proses terkait sinkronisasi dan Nexcloud API sebagai kunci utama dalam manajemen file yang lebih leluasa di Nexctloud file repository. Oleh sebab itu sistem cerdas ini diharapkan dapat melakukan pengecekan kelengkapan sekaligus mempermudah pengguna dalam merelasikan antara kalimat maupun poin-poin dalam dokumen dengan file pendukungya. Dengan demikian diharapkan bahwa dengan adanya sistem ini akan dapat membantu pihak-pihak penyusun akreditasi agar dapat memastikan bahwa kelengkapan dokumen-dokumen pendukung menjadi terjamin dengan adanya sistem yang sederhana, informatif dan selalu update ketika ada tambahan dokumen baru.

Selection of potential probiotic candidate bacteria from seawater and shrimp pond sediments for controlling the pathogenic Vibrio parahaemolyticus Zulfani, Anisa; Yuhana, Munti; Sukenda, Sukenda; Afif, Usamah
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.10-20

Vibrio parahaemolyticus is one of the pathogenic bacteria that cause vibriosis. V. parahaemolyticus strain that expresses the PirA and PirB toxins is the main causative agent of Acute Hepatopancreatic Necrosis Disease which causes necrosis (cell death) suddenly in the hepatopancreatic organs of shrimp. The environmentally friendly approach to prevent bacterial infections in shrimp is through the application of probiotics. Probiotic isolates originated from the same environment as the host and pathogen were expected to have a better adaptation and competition capability. Probiotic selection begins with the process of searching for and screening the potential probiotic candidates. This study aims to obtain isolates and characterize the potential probiotic bacteria isolated from seawater and pond sediments as an inhibition effort V. parahaemolyticus. The research was conducted experimentally using a completely randomized design with 3 treatments and 2 replicates in the in vitro antagonistic test as well as the in vivo non-pathogenicity test. The results of the study successfully obtained 37 probiotic candidate isolates with 10 isolates having the greatest enzyme activity. LAZ-2 (Acinetobacter radioresistens) isolated from seawater and SAZ-22 (Fusobacterium varium) isolated from pond sediment have been selected as probiotic candidate bacteria that have beneficial enzymatic activities. Both isolates were capable of inhibiting the growth of the pathogenic V. parahaemolyticus cells population and both were not pathogenic to vannamei shrimp. Keywords: probiotic, screening, vannamei shrimp, Vibrio parahaemolyticus ABSTRAK Vibrio parahaemolyticus merupakan salah satu bakteri patogen penyebab penyakit vibriosis. V. parahaemolyticus strain tertentu yang mengekspresikan toksin PirA dan PirB merupakan agen penyebab utama Acute Hepatopancreatic Necrosis Disease yang menyebabkan nekrosis (kematian sel) secara tiba-tiba pada organ hepatopankreas udang. Pendekatan yang ramah lingkungan untuk mencegah penyakit infeksi bakterial pada udang adalah melalui aplikasi probiotik. Isolat-isolat probiotik yang berasal dari lingkungan yang sama dengan inang dan patogen akan memiliki suatu kemampuan adaptasi dan kompetisi yang lebih baik dari yang lainnya. Seleksi probiotik diawali dengan proses pencarian dan skrining kandidat probiotik yang potensial. Penelitian ini bertujuan untuk mendapatkan isolat dan mengkarakterisasi bakteri probiotik potensial yang diisolasi dari air laut dan sedimen tambak sebagai upaya penghambatan V. parahaemolyticus. Penelitian dilakukan secara eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 2 kali ulangan pada uji antagonistik in vitro dan uji non-patogenisitas. Hasil penelitian berhasil mendapatkan 37 isolat kandidat probiotik dengan 10 isolat yang memiliki aktivitas enzim terbesar. LAZ-2 (Acinetobacter radioresistens) yang diisolasi dari air laut dan SAZ-22 (Fusobacterium varium) yang diisolasi dari sedimen tambak terpilih sebagai bakteri kandidat probiotik yang memiliki aktivitas enzim yang menguntungkan. Kedua isolat tersebut mampu menghambat pertumbuhan populasi sel patogen V. parahaemolyticus dan keduanya tidak bersifat patogenik terhadap udang vaname. Kata kunci: probiotik, seleksi, udang vaname, Vibrio parahaemolyticus

USE OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECT ANTIBODIES IN AYU (Plecogiossus altivelis) VACCINATED BY IMMERSION ADMINISTRATION Sukenda, .; Wakabayashi, Hisatsugu
Jurnal Akuakultur Indonesia Vol. 1 No. 1 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTAn indirect enzyme-linked immunosorbent assay (ELISA) was used to detect serum antibody in ayu, Plecoglossus altivelis, immunized against Pseudomonasplecoglossicida by immersion vaccination. First, the procedure of the ELISA was optimized and the sensitivity was checked. Secondly, the formalin-killed cells (FKC) of P. plecoglossicida was administered to ayu by immersion vaccination. Two weeks after vaccination, fish were divided into two groups, one group was given booster. The level of specific antibody production of both boostered and vaccinated only fish were statistically higher than unvaccinated control fish at the time of each blood collection. However, the differences between the boostered and vaccinated only fish were not statistically significant.Keywords : immunization, Pseudomonas plecoglossicida, ayu, ELISA ABSTRAKIndirect enzyme-linked immunosorbent assay (ELISA) digunakan untuk mendeteksi antibodi pada ayu, Plecoglossus altivelis, yang diimunisasi dengan cara perendaman untuk melawan infeksi Pseudomonas plecoglossicida. Pertama, prosedur ELISA dioptimasikan dan sensitivitas dari metode ini juga diperiksa. Kemudian, bakteri Plecoglossus altivelis yang sudah dimatikan dengan formalin diberikan ke ikan ayu dengan vaksinasi perendaman. Dua minggu setelah vaksinasi, ikan dibagi menjadi dua kelompok, satu kelompok diberi vaksinasi kedua. Produksi antibodi spesifik dari ikan-ikan yang divaksinasi satu kali dengan vaksinasi dua kaii secara statistik lebih tinggi dibandingkan dengan control. Akan tetapi, tidak ada perbedaan produksi antibodi antara ikan yarig divaksanisi satu kali dengan divaksinasi dua kali.Kata kunci : imunisasi, Pseudomonasplecoglossicida, ayu, ELISA

The Role of Calyx and Fruit Extract of Mangrove Sonneratia caseolaris (L) on Infection by Bacteria Vibrio harveyi in Shrimp (Penaeus monodon Fab.) Maryani, .; Dana, D.; Sukenda, .
Jurnal Akuakultur Indonesia Vol. 1 No. 3 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTBacteriological disease that caused by bacteria V. Harveyi often lead to any damage in shrimp cultivation. The study of the role of calyx and fruit extract of mangrove S. Caseolaaaris (L) for preventation and healing on infection by bacteria V. Harveyi to shrimp (P.monodon Fab) have conducted. Dosage level that applied in this study, both preventation and healing is 100 ppm and 200 ppm, respectively, obtained in the result of the preventation and healing experiment. For hemocyt, percent of granulosit increased and decrease for percent of hialin, contrastly if compared with control. Application of mangrove extract can also increase the resistence of shrimp after infectedby bacteria V. harveyi and decreasing of bacteria level in the body of shrimp. By histological observation, appearing normality of digestion system with the rich of food in digestion tract after application of mangrove S. caseolaris (L) can applicated for preventation and healing to infection of bacteria V. harveyi to shrimp.Key words : Bacterial infection, Vibrio harveyi, Sonneratia caseolaris, tiger shrimp. ABSTRAKPenyakit bakterial yang disebabkan oleh bakteri Vibrio sering menimbulkan masalah pada budidaya udang. Penelitian untuk mengetahui peranan ekstrak kelopak dan buah mangrove Sonneratia caseolaris (L), untuk pencegahan dan pengobatan terhadap infeksi bakteri Vibrio harveyi pada udang windu (Penaeus monodon Fab.) telah dilakukan. Dosis yang digunakan pada percobaan ini adalah 100 ppm untuk pencegahan dan 200 ppm untuk pengobatan. Didapatkan hasil pada percobaan pencegahan dan pengobatan untuk jenis hemosit terjadi kenaikan persentase granulosit dan penurunan persentase hialin dan bila dibandingkan dengan kontrol hal ini terjadi sebaliknya. Pemberian ekstrak mangrove juga meningkatkan ketahanan hidup udang windu setelah diinfeksi dengan bakteri V. harveyi dan penurunan jumlah bakteri yang terdapat pada tubuh udang. Gambaran pengamatan histologis memperlihatkan terjadinya kenormalan pada organ pencernaan dengan penuhnya saluran pencernaan oleh pakan setelah pemberian ekstrak mangrove. Dari hasil penelitian dapat disimpulkan bahwa ekstrak kelopak dan buah mangrove (S. caseolaris) dapat dipergunakan untuk pencegahan dan pengobatan terhadap infeksi bakteri V. harveyi pada udang windu.Kata kunci : Infeksi bakteri, Vibrio harveyi, Sonneratia caseolaris, udang windu.

The Effect of Hydroquinone Extracted from Sonneratia caseolaris Fruit on the Hemolymph of Tiger Prawn, Penaeus monodon Fab., Infected by Vibrio harveyi Arifuddin, .; Sukenda, .; Dana, D.
Jurnal Akuakultur Indonesia Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Study on the effect of hydroquinone extracted from 5. caseolaris fruit on Penaeus monodon immune system was carried out. Hydroquinone was extracted by methanol and chloroform using a soxhlet apparatus. Two kinds of in vivo experiments were conducted that were (1) shrimps were only injected with hidroquinone and (2) shrimps were injected with hidroquinone and challenged with Vibrio harveyi. Total hemocyte, differential hemocyte and phagocytic index were examined on shrimp injected with only hidroquinone and shrimp injected with hidroquinone and challenged with V. harveyi. Total hemocyte of hydroquinone injected shrimp increased significantly by day 5 from 8.33xl06 to 4,93 x 107 cells/ml. Hyaline and semi granular hemocyte cells by day 7 decreased from 61,15% to 27,84% and 8,14% to 2,53%, respectively. However, granular hemocyte cells increase significantly from 30,71% to 69,63%. Phagocytic index increased significantly by day 7 from 0.41 to 0,76. These findings indicated that crude hydroquinone can increase non spesifik imune response of P. monodon against V. harveyi infection. Key words: hydroquinone. Sonneratia caseolaris. Vibrio harveyi, Penaeus monodon, immune system ABSTRAK Kajian tentang efek hidrokuinon yang diekstraksi dari buah S. caseolaris pada sistem imun udang windu, Penaeus monodon, dilakukan. Hidrokuinon diekstraksi dengan methanol dan kloroform. Dua jenis percobaan in vivo dilakukan yaitu (1) udang hanya diinjeksi dengan hidrokuinon (2) udang diinjeksi dengan hidrokuinon kemudian diuji tantang dengan bakteri Vibrio harveyi. Total hemosit, diferensial hemosit dan indeks pagositas diperiksa pada udang yang dinjeksi dengan hidrokuinon saja dan pada udang yang diinjeksi dengan hidrokuinon dan diuji tantang dengan bakteri V. harveyi. Total hemosit meningkat secara nyata sampai hari ke 5 dari 8,33 x I06 ke 4,93 x 107 sel/ml. Hyaline dan sel-sel semi granular sampai hari ke 7 masing-masing turun dari 61,15% ke 27,84% dan 8,14% ke 2,53%. Hasil ini menunjukkan bahwa hidrokuinon dapat meningkatkan respon imun spesifik pada udang terhadap infeksi V. harveyi. Kata kunci: hidrokuinon, Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon, sistem imun

The Effect Hydroquinone Extracted from Sonneratia caseolaris Fruit to Control Vibrio harveyi Artificial Infection on Tiger Shrimp, Penaeus monodon Fab. Arifuddin, .; Sukenda, .; Dana, D.
Jurnal Akuakultur Indonesia Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The role of hydroquinone extracted from Soneratia caseolaris fruit to control Vibrio harveyi infection on tiger prawn was carried out. In vitro experiment was conducted using disc diffusion and MIC {minimum inhibitory concentration) methods to know the sensitivity of V. harveyi to hydroquinone. Two kinds of in vivo experiments were (1) hydroquinone was injected into shrimps muscle and a week later the shrimps were challenged with V. harveyi (2) the shrimps were challenged with V. harveyi and one day later hidroquinone was injected. Total count of live V. harveyi on the shrimps and survival rate were observed after challenge test. Hydroquinone showed antibacterial activity with MIC at 3000 ppm. Hydroquinone injected shrimp showed higher survival rate compared with control (100% vs 50%). Total count of V. harveyi from injected shrimp, either before or after challenged, decreased by 2,61xl04 cfu/g and l,61xl04 cfu/g, respectively. These findings indicated that crude hydroquinone have anti-bacterial effect to control V. harveyi infection. Key words: hydroquinone, Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon ABSTRAK Telaah peran hidrokuinon yang diekstraksi dari buah Soneratia caseolaris untuk mengontrol infeksi Vibrio harveyi pada udang windu dilakukan. Percobaan in vitro dilakukan menggunakan metode difusi cakram dan MIC {minimum inhibitory concentration) untuk mengetahui sensitivitas V. harveyi terhadap hidrokuinon. Percobaan in vivo dilakukan dengan dua cara (1) hidrokuinon disuntikkan pada otot udang dan seminggu kemudian udang diuji tantang dengan V. harveyi (2) udang ditantang terlebih dahulu dengan V. harveyi dan sehari kemudian hidrokuinon disuntikkan. Jumlah total bakteri V. harveyi hidup pada udang dan kelangsungan hidup udang diamati setelah uji tantang. Hidrokuinon menunjukkan aktivitas antibakteri dengan MIC 3000 ppm. Udang yang diinjeksi dengan hidrokuinon mempunyai kelangsungan hidup yang lebih tinggi dibandingkan kontrol (100% vs 50%). Jumlah total V. harveyi pada udang yang diinfeksi, baik sebelum maupun sesudah, masing-masing turun sampai 2,61 x 104 cfu/g dan 1,61 x 104 cfu/g. Hasil ini menunjukkan bahwa hidrokuinon mempunyai efek anti-bakterial untuk mengontrol infeksi V. harveyi. Kata kunci: hidrokuinon, Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon

Resistance of Fry from Vaccinated Mother of Gift Tilapia (Oreochromis niloticus Linn.) to Artificial Infection of Streptococcus iniae Nur, I.; Sukenda, .; Dana, D.
Jurnal Akuakultur Indonesia Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

An investigation was made to study the efficacy of dosage and time of administration in maternal vaccination. Mothers of nile tilapia were vaccinated by intraperitoneal injection with adjuvant-heat killed Streptococcus iniae at one and two weeks after spawning at the dosages of 0,2 ml/kg and 0,4 ml/kg body weight. Unvaccinated mothers were used as control. Agglutinating antibody titers in the blood plasma of mothers before and after spawning, eggs soluble extract, the body fluid of fry at 5, 10 and 15 days post hatching (DPH) were examined. The protective immunity of fry was tested by challenge test, the survival rate (SR) and the relative percent survival (RPS) offish within 7 days was observed. There was no difference in the antibody level of mothers, eggs soluble extract, and the body fluid of fry at 5, 10 and 15 DPH from vaccinated mothers at one or two weeks after spawning. However, antibody of mothers, eggs and fry from vaccinated mothers at the dosage of 0,4 ml/kg body weight were higher than the dosage of 0,2 ml/kg body weight and control. Antibody titre. of fry of vaccinated mothers at 5, 10 and 15 DPH were (-log2=2,88), (-log2=2,53) and (-log2=2,07) respectively, while SR were 89%, 94% and 92% respectively. SR of control fry were lower 7%, 10% and 12% respectively than fry from vaccinated mother; meanwhile RPS were 47%. 76% and 77% respectively. Key words: Maternal immunity. Streptococcus iniae, Oreochromis niloticus, vaccination, antibody ABSTRAK Suatu studi tentang efikasi dari dosis dan waktu pemberian vaksin pada vaksinasi lewat induk dilakukan. Induk ikan nila (Oreochromis niloticus) diberi vaksin melalui injeksi intraperitoneal dengan "adjuvant-heat killed Streptococcus iniae" satu minggu sesudah memijah dan dua minggu sesudah memijah dengan dosis 0,2 ml/kg dan 0,4 ml/kg bobot tubuh. Aglutinasi titer antibodi dari plasma darah induk sebelum dan sesudah memijah, ekstrak terlarut telur, dan cairan tubuh pada 5, 10 dan 15 hari setelah menetas (DPH) diperiksa. Imunitas dari larva diuji dengan uji tantang, kelangsungan hidup (SR) dalam 7 hari setelah uji tantang diamati. Hasil menunjukkan bahwa tidak ada perbedaan pada level antibodi dari induk, ekstrak terlarut telur dan cairan tubuh dari larva pada 5, 10 dan 15 DPH dari induk yang diberi vaksin pada satu atau dua minggu setelah memijah. Akan tetapi, level antibodi induk, ekstrak terlarut telur dan cairan tubuh larva dari induk-induk yang diberi vaksin dengan dosis 0.4 ml/kg bobot tubuh lebih tinggi dari 0.2 ml/kg bobot tubuh dan kontrol. Level antibodi dari larva yang berasal dari induk yang diberi vaksin dengan dosis 0.2 ml/kg, 0.4 ml/kg dan kontrol adalah (-log2=2.5), (-log2=2.9) dan (-log2=2). Sedangkan kelangsungan hidupnya masing-masing 95%, 94% dan 84%. Kata kunci: Imunitas induk. Streptococcus iniae, Oreochromis niloticus, vaksinasi, antibodi

Screening of probiotic bacteria and its role on artificial infection of Vibrio harvey in white shrimp (Litopenaeus vannamei) Sukenda, .; Sihombing, A.J.; Novianti, Fitria; Widanarni, .
Jurnal Akuakultur Indonesia Vol. 4 No. 2 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Probiotic was screened from 28 strains of normal bacterial flora isolated from rearing water in a Litopenaeus vannamei farm based on its inhibitory activity against the growth of Vibrio harveyi. Antibacterial activity was also tested in vivo to V. harveyi in L. vannamei. The result showed that the probiotic has a antibacterial effect on V. harveyi. The in vivo test showed that shrimps injected with probiotic previously before challenged with V. harveyi has survival higher than control. Probiotic isolate was suspected as Vibrio furnissi. Keywords: biocontrol, inhibitory activity, Vibrio furnissi, Vibrio harveyi, Litopenaeus vannamei AbstraK Bakteri probiotik ditapis dari 28 strain bacteria flora yang diisolasi dari air pemeliharaan udang vaname Litopenaeus vannamei berdasarkan aktivitas penghambatannya terhadap pertumbuhan Vibrio harveyi. Aktivitas bakteri probiotik juga diuji secara in vivo terhadap V. harveyi pada udang putih. Hasil penelitian menunjukkan bahwa bakteri probiotik isolat memiliki kemampuan antibakteria terhadap V. harveyi. Uji in vivo menunjukkan bahwa udang yang diinjeksi probiotik sebelum diuji tantang dengan V. harveyi memiliki kelangsungan hidup lebih tinggi daripada kontrol. Isolat probiotik tersebut diduga adalah Vibrio furnissi. Kata kunci: biokontrol, aktivitas penghambatan, Vibrio furnissi, Vibrio harveyi, Litopenaeus vannamei

Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Rahman . Sunarto A. Hasan A. Santika A. Suwanto A.J. Sihombing Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Aldy Mulyadin Alimuddin Alit Brilliant Aminatul Zahra ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anis Nugrahawati Annisa Astri Anggraeni Ayi Santika Badrudin, Deni Ramdani Bambang Gunadi Benny Yustim Brite, Margie Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah, Dendi Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Eka Angga Laksana Enang Harris Enang Harris Esti Handayani Hardi Evan Farhan Wahyu Puadi F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadlilah, Rizqy Aditya Fitria Novianti Gustilatov, Muhamad Henky Manoppo Hisatsugu Wakabayashi I. Normalina I. Nur Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Kukuh Nirmala L. Jamal Lastriliah, Mira M. Yuhana M. Zairin Junior M.S. Arifin Mahendra, Randa Mia Setiawati Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA Muttaqin, Helmy Faisal N.A. Maswan Nasri Julaini Nasrullah, Hasan Nur Bambang Priyo Utomo Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pras, Eva Prasetiyono Pratiwi, Kiki Amalia R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Septiani, Ghita Ryan Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Tatag Budiardi Trian Rizky Febriansyah Tsani Untsa, Agista Uba Umbara Ulil Surtia Zulpratita Umbara, Uba Wesly Pasaribu WIDANARNI WIDANARNI Wisriati Lasima Y. Tri Anggoro Yan Evan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa

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