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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Veteriner e-Journal BUDIDAYA PERAIRAN Jurnal Natur Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) OCTOPUS : Jurnal Ilmu Perikanan Jurnal Ilmiah KOMPUTASI Omni-Akuatika Indonesian Aquaculture Journal JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan Brilliance: Research of Artificial Intelligence Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Algoritma Jurnal Kedokteran Hewan Strata Law Review Policy Brief Pertanian, Kelautan, dan Biosains Tropika Journal of Fisheries & Marine Jurnal Matematika Ilmiah Universitas Muhammadiyah Kuningan

Sukenda .

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680

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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Effect of Sucrose as Carbon Source and Probiotic Administrations on Bacterial Population Dinamic and Water Quality in White Shrimp, Litopenaeus vannamei Culture Sukenda, .; Hadi, P.; Harris, E.
Jurnal Akuakultur Indonesia Vol. 5 No. 2 (2006): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Disinfection and nutrient enrichment prior stocking of post larvae in the pond will be affected on the growth and composition of microbe. Attention should be taken to some factors related to deterministic and stochastic factors of aquaculture environment in order to develop microbe community. This study was performed to determine effect of sucrose and probiotic supplementation to shrimp culture pond on water quality profile and population dynamic on shrimp culture media. The treatments were supplementation of sucrose as carbon source, probiotic, and sucrose + probiotic into 25 L culture medium containing white shrimp, Litopenaeus vannamei. Shrimp were fed commercial diet containing 30% protein by 5% body weight every day. The result of study showed that bacterial population was increased by increasing time of shrimp rearing. Increased of bacterial population was contrary to DO value. Bacteria grew was heterotrop and vibrio that its intensity varied during experiment. Supplementation of sucrose supported proliferation of bacteria including heterotrop, probiotik and vibrio groups. Specifically, interaction between probiotic bacteria and vibrio was also found. The presence of probiotic bacteria showed a negative impact on vibrio population. Further, development of bacteria in general was also implicated to fluctuation of ammonia concentration in pond. Keywords: carbon, sucrose, probiotic, white shrimp, Litopenaeus vannamei ABSTRAK Kegiatan disinfeksi dan pengkayaan nutrien sebelum penebaran PL akan mempengaruhi pola pertumbuhan dan komposisi mikroba di tambak. Hal-hal yang perlu diperhatikan dalam pengembangan komunitas mikroba adalah faktor-faktor deterministic dan sthocastic masing-masing lingkungan budidaya. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian bahan berkarbon (sukrosa) dan probiotik di tambak terhadap profil kualitas air serta dinamika populasi pada perairan budidaya. Pada penelitian ini dilakukan penambahan sumber karbon (sukrosa), penambahan probiotik dan penambahan sukrosa + probiotik pada masing-masing wadah yang berisi 25 liter air dan udang Vanamei, Litopenaeus vannamei. Pakan yang diberikan berupa pellet komersial dengan kadar protein 30% setiap hari sebanyak 5% dari biomassa awal. Dari hasil penelitian ini diketahui bahwa populasi bakteri pada media budidaya meningkat seiring dengan bertambahnya waktu pemeliharaan. Peningkatan jumlah populasi bakteri ini diikuti oleh semakin menurunnya nilai DO secara umum. Selain bakteri heterohof, tumbuh juga bakteri golongan Vibrio dengan persentase kemunculan yang berbeda pada setiap perlakuan. Penambahan sukrosa dalam media budidaya mendukung proliferasi bakteri secara umum, heterotrof, probiotik dan golongan Vibrio. Secara spesifik, timbul juga interaksi antara bakteri probiotik dengan bakteri Vibrio. Tumbuhnya bakteri probiotik berimplikasi negatif terhadap populasi Vibrio. Selain itu pertumbuhan bakteri secara umum juga berimplikasi terhadap fluktuasi kadar amonia perairan. Kata kunci : karbon, sukrosa, probiotik, udang vaname, Litopenaeus vannamei

Use of Chitosan to Control Vibrio harveyi Infection on White Shrimp Litopeneaus vannamei Sukenda, .; Tri Anggoro, Y.; Wahjuningrum, D.; Rahman, .
Jurnal Akuakultur Indonesia Vol. 6 No. 2 (2007): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Immunostimulation and antibacterial effect of chitosan against vibriosis were examined in white shrimp (Litopenaeus vannamei). Control shrimps were injected with 0.05 μl of sterile sea water, while experimental shrimps were injected with 2, 4 and 6 μg per g shrimp of chitosan. All shrimps were subsequently challenged by 106 CFU/ml of live Vibrio harveyi by injection method. Survival rate of shrimps injected with chitosan were found to slightly increase in accordance with dose of chitosan, even not statistically significant. Total haemocyte count and phagocytic index at experimental shrimps were over than control shrimps up to three days post injection. Number of V. harveyi in the intestine of experimental shrimps were lower than control shrimps indicates an antibacterial activity of chitosan to combat infection. Keywords: chitosan, Vibrio harveyi, haemocyte, phagocytic index, Litopenaeus vannamei ABSTRAK Efek imunostimulasi dan antibakterial dari kitosan melawan vibriosis dilihat pada udang putih (Litopenaeus vannamei). Udang control disuntik dengan 0,05 μl air laut steril, sedangkan udang uji disuntik dengan kitosan 2, 4 dan 6 μg per g udang. Semua udang diuji tantang dengan 106 CFU/ml bakteri Vibrio harveyi hidup dengan metode penyuntikan. Sintasan udang yang disuntik dengan kitosan meningkat berbarengan dengan peningkatan dosis kitosan, meskipun tidak menunjukkan perbedaan yang nyata. Jumlah total hemosit dan indeks fagositosis pada udang lebih tinggi dibandingkan kontrol sampai tiga hari pasca penyuntikan. Jumlah V. harveyi dalam saluran pencernaan dari udang uji lebih rendah dibandingkan udang kontrol, hal ini menunjukkan aktifitas antibacterial dari kitosan dalam melawan infeksi. Kata kunci: kitosan, Vibrio harveyi, hemosit, indeks fagositosis, Litopenaeus vannamei

Potency of Garlic (Allium sativum) Extract Against Motile Aeromonad Septicaemia Disease Caused by Aeromonas hydrophila in Pangasionodon hypophthalmus Yuhana, M.; Normalina, I.; Sukenda, .
Jurnal Akuakultur Indonesia Vol. 7 No. 1 (2008): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

An infectious disease caused by motile members of the genus Aeromonas, is among the most common diseases of fish cultured in freshwater, including the Thai catfish, Pangasionodon hypophthalmus. Motile aeromonad infections have been recognized for many years by various names, including motile aeromonad septicemia (MAS), motile aeromonad infection (MAI), hemorrhagic septicemia, red pest, and red sore. In our experiment, we study the potency of a phytopharmacia agent, garlic extract for prevention and curing off the experimental infections by Aeromonas hydrophila. In series of in vitro assays, the garlic extract showed inhibition capacity on the bacterial growth. The extract dosage of 25 mg/ml (2.5 g/l) was found to be the most effective among other dosages i.e 1, 2, 5, 10, 20, 50, respectively. Whereas in an in vivo assay using 25 mg/ml, it showed to be the most effective dosage for prevention against the intramuscularly experimental injection of A. hydrophila rather than as a curing agent. Keywords: Pangasionodon hypophthalmus, Allium sativum, dan Aeromonas. ABSTRAK Salah satu penyakit yang sering menyerang ikan patin (Pangasionodon hypophthalmus) dan ikan air tawar lainnya adalah penyakit MAS (Motile Aeromonad Septicemia). Penyakit ini juga dikenal sebagai penyakit bercak merah yang disebabkan oleh bakteri Aeromonas hydrophila dan mudah menular. Secara in vitro, ekstrak bawang putih (Allium sativum) berpotensi sebagai antibakteri yang dapat menghambat pertumbuhan bakteri A. hydrophila. Dosis ekstrak bawang putih sebesar 25 mg/ml (2,5 g/l) merupakan dosis yang efektif untuk menghambat pertumbuhan A. hydrophila pada uji in vitro. Pada uji in vivo ekstrak bawang putih yang disuntikkan terhadap ikan patin, dengan dosis ekstrak sebesar 25 mg/ml (2,5 g/l) menunjukkan hasil yang lebih efektif dalam mencegah infeksi A. hydrophila dibandingkan pengobatan. Kata kunci: patin, Pangasionodon hypophthalmus, bawang putih, Allium sativum, MAS dan Aeromonas.

Use of Chitosan to Prevent Aeromonas hydrophila Infection on Catfish Clarias sp. Sukenda, .; Jamal, L.; Wahjuningrum, D.; Hasan, A.
Jurnal Akuakultur Indonesia Vol. 7 No. 2 (2008): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Immunostimulation effect of chitosan against motile aeromonas septicemia caused by Aeromonas hydrophila were examined in catfish (Clarias sp.). Experimental fish were injected with 2, 4 and 6 μg/g fish of chitosan. All fish were subsequently challenged by 105 CFU/ml of live A. hydrophila by injection method. Negative control injected with PBS and positive control injected with only A. hydrophila were included in the experiment. Results showed that total count of eritrocyte, leucocyte, level of hematocrite, haemoglobin and phagocytic index higher at fish injected with chitosan previously compared with control as well as lymphocyte, neutrophile, monocyte, and trombocyte. Either survival rate or growth of fish injected with chitosan were found to increase in accordance with dose of chitosan. Kata Kunci : chitosan, Aeromonas hydrophila, immunostimulant, Clarias sp. ABSTRAK Efek imunostimulasi dari kitosan melawan Motile Aeromonad Septicemia yang disebabkan oleh A. hydrophila dilihat pada ikan lele (Clarias sp.). Ikan uji disuntik dengan larutan kitosan dengan dosis 2, 4 and 6 μg/g, yang selanjutnya diuji tantang dengan bakteri A. hydrophila 105 CFU/ml melalui penyuntikan intramuskular. Kontrol negative disuntik dengan PBS dan control positif disuntik hanya dengan bakteri A. hydrophila disertakan dalam penelitian ini. Hasil menunjukkan bahwa jumlah eritrosit, lekosit, level hematokrit, hemoglobin dan indeks fagositik lebih tinggi pada ikan-ikan yang diberi kitosan sebelumnya dibandingkan dengan tanpa pemberian kitosan sebelumnya. Begitu pula dengan kadar limfosit, netrofil, monosit dan trombosit. Sintasan dan pertumbuhan ikan yang diberi kitosan meningkat sejalan dengan dosis kitosan yang diberikan. Kata kunci: kitosan, Aeromonas hydrophila, immunostimulan, Clarias sp.

Inhibitory Mechanism of Robiotic Bacteria on The Growth of Vibrio harveyi in Tiger Shrimp (Penaeus monodon) Larvae Widanarni, .; Ayuzar, E.; Sukenda, .
Jurnal Akuakultur Indonesia Vol. 7 No. 2 (2008): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Three probiotics named SKT-b, 1Ub, and Ua had inhibitory activity against the growth of Vibrio harveyi. These strains were mutated by rifampicin resistant. The inhibitory effect of SKT-b,1Ub, and Ua on the growth of V. harveyi was investigated by concomitant incubation of the two bacteria in a culture shrimp larvae. Colony forming unit of V. harveyi, probiotic and total of bacteria in dead, live larvae and water culture was monitored, and survival rate of larvae was investigated. Shrimp inoculated probiotic previously had survival rate higher than control (without probiotic). Number of V. harveyi in treatment without probiotic inoculation also higher compared to treatment with probiotic inoculation in dead, live larvae and water culture. It demonstrated possible inhibition of probiotic bacteria on V. harveyi through competition for adherence sites or nutrition source. Partial sequencing of 16S-rRNA gene showed that 1Ub was similar to Pseudoalteromonas piscicida, whereas SKT-b and Ua were similar to Vibrio alginolyticus. Keywords: probiotic bacteria, inhibitory mechanism, V. harveyi, tiger shrimp ABSTRAK Tiga isolat bakteri probiotik yaitu 1Ub, SKT-b dan Ua telah diuji memiliki aktivitas penghambatan terhadap pertumbuhan V. harveyi secara in vitro. Ketiga isolat ini kemudian diberi penanda resisten rifampisin (RfR) melalui mutasi spontan untuk mempelajari mekanisme penghambatannya pada larva udang windu. Efek penghambatan dari 1Ub, SKT-b dan Ua terhadap pertumbuhan V. harveyi diamati melalui pemberian secara bersamaan antara bakteri probiotik dan V. harveyi tersebut dalam air pemeliharaan larva udang. Jumlah sel bakteri probiotik, V. harveyi dan total bakteri baik pada larva mati, larva hidup dan air pemeliharaan diamati dan kelangsungan hidup larva dihitung. Nilai kelangsungan hidup udang pada perlakuan yang diinokulasi bakteri probiotik lebih tinggi daripada kontrol (tanpa penambahan bakteri probiotik). Jumlah sel V. harveyi pada perlakuan tanpa penambahan probiotik juga lebih tinggi, dibanding pada perlakuan dengan penambahan probiotik baik pada larva mati, larva hidup maupun air media pemeliharaan. Hal ini menunjukkan adanya penghambatan bakteri probiotik terhadap V. harveyi yang kemungkinan melalui kompetisi tempat pelekatan atau sumber nutrisi. Hasil analisis sekuen sebagian gen 16-rRNA menunjukkan bahwa isolat 1Ub termasuk spesies Pseudoalteromonas piscicida, sedangkan SKT-b dan Ua termasuk spesies Vibrio alginolyticus. Kata kunci: bakteri probiotik, mekanisme penghambatan, V. harveyi, udang windu

Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker Widanarni, .; Meha, D.; Nuryati, Sri; Sukenda, .; Suwanto, A.
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Rifampicin-resistant marker was employed as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae. V. harveyi M. G3 and G7 that difference not schizotyping as shown by Pulsed-Filed Gel Electrophoresis (PFGE) used in this study. Spontaneous mutation was conducted to generate V. harveyi resistant to rifampicin. Two groups of shrimp post-larvae (PL5) were immersed for 30 min in 106 CFU/ml of mutants and wild type of V. harveyi, respectively; and then placed in a 2 liter shrimp rearing tank for five days. A control group was immersed in sterile seawater. Growth curve analysis and pathogenicity assay of V. harveyi showed that each of the V. harveyi mutant exhibited almost identical profiles to that of the wild type parental strain and did not show alteration in their pathogenicity. Sample from dead shrimp larvae showed that the dead shrimp larvae were infected by V. harveyi RfR, indicated that rifampicin-resistant marker effective as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae. Key words: shrimp larvae, Vibrio harveyi, rifampicin-resistant, molecular marker

The potential of Trichoderma sp. as antibacterial and immunostimulant on white shrimp (Litopenaeus vannamei) Pebrianto, Catur A.; Sukenda, .; Widanarni, .
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The objective of this research was to study antibacterial and immunostimulatory effects of Trichoderma sp. extract on white shrimp, Litopenaeus vannamei. First experiment was conducted to evaluate inhibitory effect of Trichoderma sp. againts Vibrio harveyi, a pathogenic bacteria causing vibriosis disease on shrimp. Second experiment was conducted to evaluate immunostimulatory effect of Trichoderma sp. on shrimp immunity as well as protective effect against V. harveyi. A group of shrimp was injected with a minimum inhibitory concentration obtained at first experiment, and a week after, shrimps was challenged with V. harveyi (prophylactic). Another group was previously challenged with V. harveyi, and subsequently injected with Trichoderma sp. two fold of Minimum Inhibitory Concentration (MIC) a day after (therapeutic). Positive control, that was received only V. harveyi, and negative controls, that was received neither Trichoderma sp. nor V. harveyi were included in this experiment. Results of first experiment showed that a concentration of 600 ppm was a MIC of Trichoderma sp. to inhibit V. harveyi. While in the second experiment, the groups receiving Trichoderma sp., either prophylactic or therapeutic, showed protective effect against V. harveyi significantly higher than positive control and lower compared with negative control. Total haemocyte count (THC), differential haemocyte count (DHC), phagocytic index and phenoloxydase activity were different among the groups of prophylactic treatment or therapeutic treatment compared to control positive and negative treatments. In conclusion, Trichoderma sp. could be used in prophylactic and therapeutic treatments to combat infection of V. harveyi on L. vannamei. Key words: Trichoderma sp., V. harveyi, immunostimulant ABSTRAK Penelitian ini bertujuan untuk mempelajari pengaruh antibakterial dan immunostimulasi ekstrak Trichoderma sp. terhadap udang putih, Litopenaeus vannamei. Percobaan pertama dilakukan untuk mengevaluasi pengaruh penghambatan Trichoderma sp. terhadap Vibrio harveyi, bakteri patogen yang menyebabkan penyakit vibriosis pada udang. Percobaan kedua dilakukan untuk mengevaluasi pengaruh immonustimulasi Trichoderma sp. terhadap imunitas udang serta pengaruh protektif terhadap V. harveyi. Sekelompok udang disuntik dengan konsentrasi hambatan minimum (minimum inhibitory concentration, MIC) yang diperoleh dari hasil percobaan pertama, dan seminggu kemudian udang diuji tantang dengan V. harveyi (profilaksis). Kelompok udang lain diuji tantang dengan V. harveyi sebelumnya untuk kemudian disuntik dengan Trichoderma sp. (therapeutic). Kontrol positif, yang hanya diuji tantang dengan V. harveyi, dan kontrol negatif, yang tidak mendapat baik Trichoderma sp. maupun V. harveyi. Hasil percobaan pertama menunjukkan bahwa 600 ppm merupakan konsentrasi MIC Trichoderma sp. yang memberikan efek penghambatan maksimal terhadap V. harveyi. Sedangkan hasil percobaan kedua, kelompok udang yang mendapatkan Trichoderma sp. baik sebagai profilaksis maupun therapeutic menunjukkan bahwa Trichoderma sp. memberikan efek perlindungan terhadap infeksi V. harveyi secara signifikan lebih tinggi daripada kontrol positif dan lebih rendah daripada kontrol negatif. Total hemocyte count (THC) dan differential hemocyte (DHC), indeks fagositik dan aktivitas fenoloksidase kelompok perlakuan profilaksis atau perlakuan therapeutic berbeda baik dengan kontrol positif maupun negatif. Untuk itu dapat disimpulkan bahwa Trichoderma sp. dapat digunakan dalam tindakan pencegahan dan pengobatan infeksi V. harveyi pada udang putih. Kata kunci: Trichoderma sp., V. harveyi, immunostimulan

Hematology of common carp following DNA vaccination and koi herpesvirus challenge test Nuryati, Sri; Maswan, N.A.; Alimuddin, .; Sukenda, .; Sumantadinata, K.; Pasaribu, F.H.; Soejoedono, R.D.; Santika, A.
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The study was aimed to determine the effectiveness of DNA vaccine doses on hematological aspect which represent immune response and its influence on common carp survival rate. DNA vaccines encoding the viral glycoprotein of koi herpesvirus (KHV) have been proved to highly protect the fish under laboratory condition. A dose of 12.5 µg/100 µl vaccine had resulted in a survival rate of 96.67 % during 30 days after challenge test with a lethal dose of KHV. Fish vaccinated using lower doses, i.e. 2.5 and 7.5 µg/100µl showed 100% mortality after 15 and 19 days challenge test respectively, whereas non vaccinated fish as a control showed 100% mortality after 17 days challenge test. Total leucocytes of the vaccinated fish were higher than control until 42 days post vaccination, but declined afterward. Phagocytic index of the vaccinated fish using 12.5 µg/100 µl was declined after 49 days post vaccination or 7 days post challenge test. Key words: DNA vaccine, Koi herpesvirus (KHV), leucocyte, phagocytic index, Cyprinus carpio ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh vaksinasi menggunakan vaksin DNA dengan dosis berbeda terhadap gambaran darah ikan sebagai respresentasi tanggap kebal ikan mas serta pengaruhnya terhadap tingkat kelangsungan hidup ikan mas. Vaksin DNA penyandi glikoprotein koi herpesvirus (KHV) dapat memberikan proteksi yang tinggi pada percobaan skala laboratorium. Vaksinasi dengan dosis 12,5 µg/100µl dapat mempertahankan kelangsungan hidup sebesar 96,67% selama satu bulan setelah uji tantang dengan virus KHV menggunakan dosis letal. Ikan yang divaksin dengan dosis yang lebih rendah yaitu 2,5 dan 7,5 µg/100µl mengalami kematian total berturut-turut setelah 15 dan 19 hari uji tantang, sedangkan ikan kontrol yang tidak divaksin mengalami kematian total setelah 17 hari uji tantang. Jumlah leukosit total ikan yang divaksinasi lebih tinggi dibanding dengan kontrol sampai hari ke-42, setelah itu mengalami penurunan. Indeks fagositosis ikan yang divaksin dengan dosis 12,5 µg/100µl mengalami penurunan setelah hari ke-49 atau 7 hari setelah uji tantang. Kata kunci: Vaksin DNA, Koi herpesvirus (KHV), leukosit, indeks fagositosis, Cyprinus carpio

Effect of SKT-b Vibrio probiotic bacteria addition at different developmental stages on tiger shrimp Penaeus monodon larvae survival rate Widanarni, .; Arifin, M.S.; Sukenda, .
Jurnal Akuakultur Indonesia Vol. 8 No. 2 (2009): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to study the effect of probiotic bacteria SKT-b Vibrio addition at different developmental stages on survival rates of tiger shrimp Penaeus monodon larva. Main activity of this experiment consisted of tiger shrimp rearing started from nauplius stage until Pl10 and addition of 106 CFU/ml SKT-b Vibrio probiotic bacteria at various developmental stages namely at early nauplius stage, early zoea zoea stage, early mysis stage, early postlarva (Pl) stage, every developmental stage changes, everyday, and control (without probiotic bacteria addition). Results showed that survival rates of shrimp larva ranged at 24,17%-35,83% with the higest value in the treatment of probiotic bacteria addition at every developmental stage changes namely 35,83%, whereas the lowest was found at control (without probiotic bacteria addition) namely 24,17%. No significantly different was found in term of shrimp growth rate among control and treatment. Growth rate in length of tiger shrimp larva ranged 18,64%-19,09% for SKT-b Vibrio addition and 18,47% for control. Key word: Probiotic bacteria, SKT-b Vibrio, Penaeus monodon, shrimp larvae stages ABSTRAK Penelitian ini dilakukan untuk mempelajari pengaruh pemberian bakteri probiotik Vibrio SKT-b pada stadia yang berbeda terhadap kelangsungan hidup larva udang windu Penaeus monodon. Kegiatan utama dari penelitian ini adalah pemeliharaan udang windu yang dimulai dari stadia nauplius sampai Pl10 dan diberi bakteri probiotik Vibrio SKT-b dengan dosis 106 CFU/ml pada waktu yang berbeda yaitu pada awal stadia nauplius, awal stadia zoea, awal stadia mysis, awal stadia postlarva (Pl), setiap pergantian stadia, setiap hari, dan kontrol (tanpa pemberian bakteri probiotik). Hasil penelitian yang diperoleh menunjukkan bahwa tingkat kelangsungan hidup larva udang berkisar antara 24,17%-35,83% dengan nilai tertinggi terdapat pada perlakuan pemberian bakteri probiotik pada setiap pergantian stadia yaitu sebesar 35,83%, sedangkan terendah pada perlakuan kontrol (tanpa pemberian bakteri probiotik) yaitu 24,17%. Sedangkan terhadap pertumbuhan tidak terdapat perbedaan yang signifikan antara kontrol dengan perlakuan. Nilai laju pertumbuhan panjang larva udang windu dengan penambahan bakteri probiotik Vibrio SKT-b berkisar antara 18,64%-19,09% dan kontrol sebesar 18,47%. Kata kunci: Probiotik, Vibrio SKT-b, Penaeus monodon, stadia larva udang

Enhancement of non-specific immune response, resistance and growth of (Litopenaeus vannamei) by oral administration of nucleotide Manoppo, Henky; Sukenda, .; Djokosetiyanto, Daniel; Sukadi, Mochamad Fatuchri; Harris, Enang
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This research evaluated the nonspecific immune responsse, resistance, and growth of Litopenaeus vannamei fed nucleotide diet. Shrimp juveniles (mean weight 5.39±0.56 g) were reared in two groups of glass aquaria, each with three replications. Shrimps in group one and group two were fed nucleotide diet and basal diet each for four weeks. Total haemocyte count (THC) and PO activity were evaluated at the end of feeding while growth was measured at two weeks interval. At the end of feeding trial, the shrimps were intramuscularly injected with Vibrio harveyi 0.1x106 cfu.shrimp-1. THC of shrimp fed nucleotide diet significantly increased (P

Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Rahman . Sunarto A. Hasan A. Santika A. Suwanto A.J. Sihombing Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Aldy Mulyadin Alimuddin Alit Brilliant Aminatul Zahra ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anis Nugrahawati Annisa Astri Anggraeni Ayi Santika Badrudin, Deni Ramdani Bambang Gunadi Benny Yustim Brite, Margie Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah, Dendi Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Eka Angga Laksana Enang Harris Enang Harris Esti Handayani Hardi Evan Farhan Wahyu Puadi F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadlilah, Rizqy Aditya Fitria Novianti Gustilatov, Muhamad Henky Manoppo Hisatsugu Wakabayashi I. Normalina I. Nur Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Kukuh Nirmala L. Jamal Lastriliah, Mira M. Yuhana M. Zairin Junior M.S. Arifin Mahendra, Randa Mia Setiawati Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA Muttaqin, Helmy Faisal N.A. Maswan Nasri Julaini Nasrullah, Hasan Nur Bambang Priyo Utomo Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pras, Eva Prasetiyono Pratiwi, Kiki Amalia R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Septiani, Ghita Ryan Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Tatag Budiardi Trian Rizky Febriansyah Tsani Untsa, Agista Uba Umbara Ulil Surtia Zulpratita Umbara, Uba Wesly Pasaribu WIDANARNI WIDANARNI Wisriati Lasima Y. Tri Anggoro Yan Evan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa

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