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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Farmasains : Jurnal Farmasi dan Ilmu Kesehatan JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK The Journal for Technology and Science Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Cancer Chemoprevention Journal of Engineering and Technological Sciences Medika Tadulako INDONESIAN JOURNAL OF PHARMACY Jurnal Fitofarmaka Indonesia Syntax Literate: Jurnal Ilmiah Indonesia Majalah Farmaseutik JRB-Jurnal Riset Bisnis Fair Value: Jurnal Ilmiah Akuntansi dan Keuangan JURNAL PENDIDIKAN TAMBUSAI Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Journal of halal product and research (JHPR) Daengku: Journal of Humanities and Social Sciences Innovation Kalbisocio: Jurnal Bisnis dan Komunikasi Makara Journal of Science Makara Journal of Technology Ekobisman : Jurnal Ekonomi Bisnis Manajemen

Wahono Sumaryono

Universitas Pancasila

Author-ID : 392209

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PERANAN RIZOBAKTERI DAN FUNGI MIKORIZA ARBUSKULAR DALAM MENINGKATKAN EFISIENSI PENYERAPAN HARA SORGUM MANIS (Sorghum bicolor L. Moench) Bedah Rupaedah; Iswandi Anas; Dwi Andreas Santosa; Wahono Sumaryono; Sri Wilarso Budi
Jurnal Ilmu Tanah dan Lingkungan Vol 16 No 2 (2014): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Efisiensi pemakaian pupuk kimia dapat didefinisikan berdasarkan aspek agronomi. Secara umum, semakin banyak hara yang diserap tanaman untuk meningkatkan hasil panen, maka tingkat efisiensi semakin tinggi. Pelacakan jumlah hara yang dapat diserap tanaman dalam meningkatkan produksi adalah komponen kunci untuk mengukur efisiensi hara. Percobaan lapangan dilakukan untuk menilai peranan rizobakteri dan fungi mikoriza arbuskular (FMA) pada berbagai konsentrasi pupuk kimia pada budidaya sorgum manis (Sorghum bicolor L. Moench). Dua galur rizobakteri (Mycobacterium senegalense 73LR dan Bacillus firmus 80JR) serta dua galur FMA (Gigaspora sp. 40MDL dan Glomus sp. 38MDL) digunakan sebagai inokulan dengan penambahan beberapa variasi konsentrasi pupuk kimia. Bobot biomasa, kandungan gula, serapan fosfor, kalium dan nitrogen dianalisis. Inokulasi rizobakteri mampu meningkatkan tinggi tanaman dan kandungan P pada taraf p<0.01, sedangkan bobot biomassa, kandungan gula, serapan P dan K pada taraf p<0.05. Inokulasi FMA saja berpengaruh nyata terhadap bobot biomassa dan kandungan N pada taraf p<0.01, sedangkan kandungan gula pada taraf p<0.05. Sementara itu, interaksi rizobakteri dan FMA mampu meningkatkan bobot biomassa, kandungan dan serapan K, serta efisiensi penyerapan hara N. Secara keseluruhan pengaruh inokulasi rizobakteri, FMA dan pupuk kimia serta interaksi faktor-faktor tersebut dapat meningkatkan bobot biomasa, kandungan gula, serapan P dan K, serta efisiensi penyerapan hara N oleh tanaman sorgum manis. Dengan demikian, pemakaian rizobakteri dan FMA berpotensi meningkatkan efisiensi penggunaan pupuk kimia, sehingga diharapkan dapat mengurangi biaya produksi budidaya sorgum manis.

The Power Consumption Performance of an Orbiting Screw Solid-Solid Mixer Semuel Pati Senda; Renanto Renanto; Achmad Roesyadi; Wahono Sumaryono; Yazid Bindar
Journal of Engineering and Technological Sciences Vol. 44 No. 3 (2012)
Publisher : Institute for Research and Community Services, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/itbj.eng.sci.2012.44.3.6

In this work we have investigated mixing in a modified orbiting screw mixer (MOSM) designed for solid-solid mixing. Mixing was carried out using urea powder and natural zeolite powder (UZ) of three varying particle sizes (50-60, 60-80 and 80 mesh). Power consumption was calculated from the measured torque of orbit and screw, obtained from computerized records. It was found that the mixing process in the modified orbiting screw mixer with air injection required a lower power consumption for each particle-size group when compared to mixing without air injection. With UZ mixing in MOSM with air injection, the lowest E was obtained for the 60-80 mesh particle-size group (4,297 Joule/kg-1),whereas when mixing without air injection, the value was 10,296 J/kg. The best mixing operation in this experiment was achieved at NFr = 1.18x10-3 and in the range of values NReâ‰ˆ 8.77x107 to 2.63x108. Moreover, in this study, we have developed an equation to estimate the power consumption required for mixing and determined its correlation with dimensionless numbers.

PENGARUH BAURAN PEMASARAN JASA DAN KUALITAS PELAYANAN KESEHATAN PUSKESMAS BOJONG NANGKA TERHADAP KEPUASAN PASIEN BPJS Novi Henriyati Rahmi; Sampurno Sampurno; Wahono Sumaryono
Media Farmasi: Jurnal Ilmu Farmasi Vol 16, No 1: Maret 2019
Publisher : Universitas Ahmad Dahlan

Penelitian ini bertujuan untuk menganalisa bagaimana pengaruh bauran pemasaran jasa dalam hal ini produk (product), harga (price), promosi (promotion), lokasi (place), orang (people), bukti fisik / fasilitas fisik (physical evidence), dan proses (process), dan kualitas pelayanan kesehatan dalam hal ini bukti langsung (tangibles), kehandalan (reliability), daya tanggap (responsiveness), jaminan (assurance), dan empati (empathy) di Puskesmas Bojong Nangka Kecamatan Gunung Putri Kabupaten Bogor terhadap kepuasan pasien BPJS. Metode penelitian menggunakan analisis deskriptif metode potong lintang atau cross sectional. Tempat penelitian adalah di Puskesmas Bojong Nangka, Gunung Putri, Bogor. Sampel penelitian berjumlah 100 orang. Teknik pengumpulan data yaitu kuesioner dan observasi. Analisis data penelitian yaitu dengan uji Regresi Linier Berganda dengan hasil Y1 = 1,515 + 0,064X1 + 0,073X2. Kesimpulan penelitian adalah bahwa bauran pemasaran dan kualitas pelayanan kesehatan berpengaruh secara parsial maupun bersama-sama terhadap kepuasan pasien BPJS.

Isolation and Identification of Mycorrhizosphere Bacteria and Their Antagonistic Effects Towards Ganoderma boninense in vitro YENNI BAKHTIAR; SUDIRMAN YAHYA; WAHONO SUMARYONO; MEITY SURADJI SINAGA; SRI WILARSO BUDI; TEUKU TAJUDDIN
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Basal stem rot caused by Ganoderma boninense is the most serious disease of oil palm (Elaeis guineensis) in Indonesia and it has caused major loss in palm oil production. Mycorrhizosphere bacteria offer possible advantages as biocontrol agents as they live and proliferate together with arbuscular mycorrhizal fungi, which have an ability to increase plant resistance against pathogens. A study was conducted to isolate mycorrhizosphere bacteria from spores of arbuscular mycorrhizal fungi and test their antagonistic effects against G. boninense in vitro. All bacterial isolates were identified based on 16S rDNA analysis and it revealed that eleven out of twenty mycorrhizosphere bacteria isolated were related to Bacillus with similarity ranging from 97 to 100%, whereas other isolates were identified as Pseudomonas, Streptomyces, Kocuria, Enterobacter, Brevundimonas, and Alcaligenes with similarities ranging from 96 to 100%. Fourteen out of twenty mycorrhizosphere bacteria showed a varying degree of inhibition towards the growth of G. boninense in vitro. Of these, isolate B10 (closely related to Bacillus subtilis ZJ06) showed the highest inhibitory effect followed by B17 (closely related to Bacillus subtilis N43). Therefore, these bacteria have a potential to be used as biocontrol agents to control basal stem rot disease caused by G. boninense in oil palm.

Isolation and Identification of Endophytic Fungi from Srikaya Plants (Annona squamosa) Having Potential Secondary Metabolites as Anti-Breast Cancer Activity PRASETYAWAN YUNIANTO; SYOFI ROSMALAWATI; INDRA RACHMAWATI; WAHYUDI PRIYONO SUWARSO; WAHONO SUMARYONO
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Annonaceous acetogenin was extracted from Annona squamosa (Srikaya) seeds. It has cytotoxic activity against cancer cells and lower toxicity compared to other cancer drugs. Endophyte from Annonaceae is expected to have similar extracted metabolites to the host, thus increasing the economic value. This research is a preliminary study to obtain active compounds with potential as anti-cancer agents from endophytic fungi of Srikaya plants. Four endophytic fungal strains were isolated from Srikaya plants (Annona squamosa) and identified based on 28S rDNA sequence. The isolates are SKY II.3.1, SKY I.1.2, SKY II.3.2, and SKY III.3.1, and have similarity with Fusarium sp Vega760, Fusarium sp NRRL 22354 NRRL223, Nectria rigidiuscula, and Fusarium sp BOL35, respectively. The identified isolates were fermented in liquid media for three weeks. The liquid and mycelium were extracted using ethyl acetate. Whole extract of each fermented isolate was partitioned and evaporated to obtain ethyl acetate extract. Cytotoxicity assay of ethyl acetate extract was carried out at level 100 ppm by Methyl Thyazole Tetrazolium (MTT) viability test towards MCF-7 (breast cancer cell). The result indicated that each ethyl acetate extract could inhibit the viability of cell MCF-7 with 11.34, 99.78, 91.48, and 96.84%, for SKY II.3.1, SKY I.1.2, SKY II.3.2, and SKY III.3.1, respectively. Based on the results of cytotoxicity assay on MCF-7 breast cancer cells, endophytic fungi isolates SKY I.1.2, SKY II.3.2, and SKY III.3.1 are potential as sources of anti-breast cancer compounds.

Selection of Carbon and Nitrogen Source for 8-Hydroxy 9, 12-Octadecadienoic Acid Production using Endophytic Fungi Curvularia lunata BioMCC FE-00283 ERWAHYUNI ENDANG PRABANDARI; TUN TEDJA IRAWADI; WAHONO SUMARYONO; KHASWAR SYAMSU
Microbiology Indonesia Vol. 5 No. 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Hydroxyoctadecadienoic acid (HODE) is one of hydroxy fatty acids that has anticancer activity. HODE was previously produced by chemical synthesis or bioconversion from linoleic acid. This is the first paper reported production of HODE by Curvularia lunata an endophytic fungi of Cibotium barometz. Various carbon and hydrogen sources have been tested for their effects on the production of HODE by C. lunata. Glucose, lactose, maltose, xylose, and sucrose were used as carbon sources, while yeast extract, monosodium glutamate, urea, and NH4Cl were used as nitrogen sources. Fermentation was done using 100 ml medium in 250 ml Erlenmeyer flask at 150 rpm, 28 oC for 10 days. HODE products were analyzed by high pressure liquid chromatography using C18 coloumn and eluted by gradient system of acetonitril-water from 15% to 100%. Glucose and monosodium glutamate were found to be the best carbon and nitrogen source. The optimum concentration of glucose and monosodium glutamate for the production of HODE were 10 mg L-1 and 12 mg L-1 respectively.

CYTOTOXIC PROTEIN FROM Trichosanthes cucumerina L. var anguina (L.) Haines Churiyah Zafrollah; WAHONO SUMARYONO
BIOTROPIA Vol. 17 No. 1 (2010)
Publisher : SEAMEO BIOTROP

Three proteins were discovered, they were: TF2 from fruit, TS3 from seed and TR3 from root of Trichosanthes cucumerina L. var anguina (L.) Haines by extraction with Phosphate Buffer Saline (PBS). The proteins were precipitated using 80% saturated ammonium sulphate and then were dialysed using cellophane. Furthermore, the dialysate was fractionated through gel filtration chromatography. The highest protein yield was 1.109%, resulted from TS3, then 0.356% from TR3, and the lowest was 0.014% from TF2. Characterization of the proteins using SDS-PAGE indicated that all of the protein fractions showed more than one sub unit molecular masses (Mr) in the range of 16 - 29 kDa. The toxicity (LC50) of proteins on brine shrimp lethality test were within range of 19 Âµg/ml - 25 Âµg/ml. The cytotoxicities of the TR3 and TS3 proteins on cancer cell line indicated that both of the active proteins inhibit proliferation of HeLa and K-562 cells with IC50 up to 45 Âµg/ml. Keywords : Trichosanthes cucumerina L. var anguina (L.), plant part, protein, yield, toxicity, cytotoxicity, molecular mass.

Mixing Urea and Zeolite for Slow Release Fertilizer using Orbiting Screw Mixer Semuel Pati Senda; Renanto Handogo; Achmad Roesyadi; Wahono Sumaryono
IPTEK The Journal for Technology and Science Vol 20, No 4 (2009)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v20i4.83

Slow release fertilizer (SRF) of urea is prepared by using zeolite as the matrix. Mixing of urea and zeolite is carried out in orbiting screw mixer. The effects of rotation speed and orbital speed of the mixer and particle size on power consumption, homogeneity, mixing time and specific energy consumption are evaluated. The experimental results show that higher orbital speed gives higher power consumption. Power consumption is dominated by mixer rotation motion. Smaller particle size needs higher power for mixing process. Nitrogen mass fraction ranges from 0.45 to 0.49 when mixture reaches homogeneity. The mixing time required is about 5 – 12 minutes for particle size of >60 and >80 mesh and 7 – 14 minutes for particle size of >50 mesh. At constant orbit speed, the higher the screw rotation speed, the shorter time needed to reach mixture homogeneity. Specific energy consumption of mixing process increases with decreasing particle size. For the three particle size groups of >80 mesh, >60 mesh and >50 mesh, the lowest specific energy consumption is given by combination of orbital speed of 5 rpm and rotation speed of 50 rpm; while for particle size of >60 mesh and >80 mesh, it can obtained by combination of orbital speed of 5 rpm and rotation speed of 67,5 rpm and orbital speed 5 rpm and rotation speed 30 rpm, respectively. The lowest specific energy consumptions is gained by combination of orbital and rotation speeds of 5 and 50 rpm with particle size of >50 mesh.

Activity of Trigonella foenum-graecum on Some Cell Lines Kurnia Agustini; Wahono Sumaryono; R. Micho Widyanto
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss2pp234-241

Trigonella foenum-graecum (TFG) is one of medicinal plants contains some steroidal sapogenin such as diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside, also alkaloid trigonellin, which is have many activity as antidiabetic, estrogenic and also as anti cancer. This experiment was done to explore the activity of some extract of TFG on some cell lines such as MCF7 (Human Breast Cancer Cell-line), T47D (Human Breast Cancer Cell-line), PC3 (Human Prostate Cell-line) and SKOV (Human Ovarian Carcinoma Cell-line). This assay was done using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium) methods. Results showed that ethyl acetate fraction gives the lowest IC50 than another extracts. IC50 for PC3 is 66.24 ppm, IC50 for MCF7 is 41.81 ppm, IC50 for T47D is 58.63 ppm. These datas can be used for further research to isolate the active compound from TFG.Keywords : Trigonella foenum-graecum, MCF-7, T47D, SKOV, PC3

Peranan rizobakteri dan fungi mikoriza arbuskular dalam proses fotosintesis dan produksi gula sorgum manis (Sorghum bicolor L. Moench) Role of rhizobacteria and arbuscular mycorrhizal fungi in increasing photosynthesis process and sugar production of sweet sorghum (Sorghum bicolor L. Moench) Bedah RUPAEDAH; Iswandi ANAS; Dwi Andreas SANTOSA; Wahono SUMARYONO; Sri Wilarso BUDI
E-Journal Menara Perkebunan Vol 83, No 1: Juni 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractDual inoculation effects of rhizobacteria andAMF as well as the addition of chemical fertilizerson photosynthesis were studied by analyzingprocess of CO2 gas exchange, chlorophyll content,nutrient uptake, sugar content and growth of sweetsorghum. AMF inoculation as a single cultureincreased carbon assimilation, stomatal conductance,intercellular CO2 concentration, phosphoruscontent, chlorophyll content, sugar content, plantheight, shoot weight and mycorrhizal colonization.Interaction of AMF and rhizobacteria increasedsugar content of sweet sorghum stems andpotassium content of sweet sorghum leaves,whereas its interaction with chemical fertilizerssignificantly increased chlorophyll content, sugarcontent and mycorrhizal colonization of sorghumplant roots. Interaction of AMF, rhizobacteria andchemical fertilizers increased sugar content, rootweight and mycorrhizal colonization. The use ofAMF solely or its interaction with rhizobacteriaand chemical fertilizers had a great potential inimproving photosynthesis process of sweetsorghum. The process is associated with increasingcrop productivity, such as sugar content ofsorghum which is potential as a source ofrenewable energy.AbstrakPengaruh inokulasi ganda rizobakteri danFMA dengan penambahan pupuk kimia dipelajaridengan cara menganalisis proses pertukaran gas CO2, kandungan klorofil, kandungan hara dan gula,pertumbuhan dan produktivitas sorgum manis.Inokulasi FMA sebagai kultur tunggal dapatmeningkatkan asimilasi karbon, konduktansistomata, konsentrasi CO2 interselular, kandunganfosfor, kandungan klorofil, kandungan gula, tinggitanaman, berat batang dan derajat kolonisasimikoriza. Interaksi antara rizobakteri dan FMAdapat meningkatkan kandungan gula batang dankalium daun sorgum manis, sedangkan interaksinyadengan pupuk kimia dapat meningkatkan kandunganklorofil dan gula serta derajat kolonisasimikoriza pada perakaran sorgum manis. Sementaraitu, interaksi FMA, rizobakteri dan pupuk kimiadapat meningkatkan kandungan gula batang, beratakar dan derajat kolonisasi mikoriza pada perakaransorgum manis. Penggunaan FMA baik sendirimaupun interaksinya dengan rizobakteri dan pupukkimia memiliki potensi besar dalam meningkatkanproses fotosintesis sorgum manis. Proses tersebutberkaitan dengan peningkatan produktivitas sorgummanis dalam hal ini kandungan gula sorgum manisyang berpotensi sebagai sumber energi terbarukan.

Co-Authors . Sriningsih ABDUL RAHEM Achmad Roesyadi Achmad Roesyadi AGUN ERU WIBOWO AGUNG ERU WIBOWO Agung Eru Wibowo Agus Supriyono AHMAD WIBISANA AMARILA MALIK Amarila Malik AMIR MUSADAD Amir Musadad Andika Kristian Astutiati Nurhasanah ASTUTIATI NURHASANAH Bedah Rupaedah Chaidir ., Chaidir CHAIDIR CHAIDIR Churiyah Zafrollah Defirst Ramadanty Deriawan Deriawan Derriawan Derriawan Derriawan Derriawan, Derriawan Dian Ratih Laksmitawati Diky Mudhakir Djoharsjah Djoharsjah DjumaÃ¢Â€Â™ali DjumaÃ¢Â€Â™ali, DjumaÃ¢Â€Â™ali Djuma’ali Djuma’ali Djuma’ali, Djuma’ali DUDI HARDIANTO Dudi Hardianto, Dudi DWI ANDREAS SANTOSA Dyah Primarini Dyah Primarini, Dyah Eko Sri Wahyuningsih ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI Faiq Bahfen Faizah FARIDAH FARIDAH Fitriani, Johana FITRIANIAR AMRI FRANS D. SUYATNA FRANS SUYATNA Frans Suyatna gusnidar, tutus HENDIG WINARNO Henky Lesmana Henky Lesmana IDAH ROSIDAH Idah Rosidah IDAH ROSIDAH Iha Haryani Hatta INDRA RACHMAWATI Irvan Bastian Iswandi Anas Izul Ariesta Khaswar Syamsu Kosasih Kosasih Kurnia Agustini kurnia agustini KURNIA AGUSTINI KURNIA AGUSTINI Lestari Rahayu MARLIA SINGGIH Marlia Singgih Maryam Muddin T MEITY SURADJI SINAGA MEITY SURADJI SINAGA Mirawati Sudiro Mohamad Sadikin Mohamad Sadikin Nathanael Priltius Nonot Soewarno Nonot Soewarno Novi Henriyati Rahmi Noviyantih, Noviyantih Nurjati Chairani Siregar Nurjati Chairani Siregar NURYATI CHAERANI SIREGAR Nuryati Chaerani Siregar NURYATI CHAIRANI SIREGAR Partomuan Simanjuntak Pertamawati Kartakusumah PRASETYAWAN YUNIANTO Pratiwi Pujilestari Sudarmono Prihatini, Indri R Micho Widyanto R. Micho Widyanto Rahmat Widiyanto Rahmi, Novi Henriyati Ratna, Oktaviani Renanto Handogo Renanto Handogo Rianto Setiabudy Rianto Setiabudy Rifatul Widjhati Rizki Riyadi Triwibowo ROS SUMARNY Ruth Debora Sampurno Sampurno Sampurno Sampurno Semuel Pati Senda Semuel Pati Senda Semuel Pati Senda Silvia Surini Silvia Surini Siti Hafilah Siti Kadarsih Siti Umroh Noor SRI NINGSIH Sri Ningsih Sri Widiastuti, Sri Sri Widyastuti Sri Widyastuti Sri Wilarso Budi Sudirman Yahya SUDIRMAN YAHYA Sumarno . Sumarno Sumarno SYOFI ROSMALAWATI Teuku Tajuddin Trismilah Margono TRISMILAH TRISMILAH Trismilah Trismilah Trismilah, TUN TEDJA IRAWADI Tun Tedja Irawadi TUTUS GUSDINAR Umi Athiyah VANNINA AGUSTINI VINDYATRI WIDAYANTI Wahyu Utami Wahyudi Priyono Suwarso Welfin Dysyandi Wida Rahayu Wiejayanti, Syelvi YAHDIANA HARAHAP Yahdiana Harahap Yazid Bindar Yazid Bindar YENNI BAKHTIAR YENNI BAKHTIAR Yudi Riyanto Yulius Evan Christian

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