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All Journal Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Jurnal Ilmu Kedokteran (Journal of Medical Science) Jurnal Kesehatan Andalas Berkala Ilmu Kesehatan Kulit dan Kelamin JURNAL PENGABDIAN KEPADA MASYARAKAT The Indonesian Biomedical Journal Collaborative Medical Journal (CMJ) Buletin Ilmiah Nagari Membangun Majalah Kedokteran Andalas Majalah Patologi Indonesia Bioscientia Medicina : Journal of Biomedicine and Translational Research Health and Medical Journal Andalas obstetrics and gynecology journal Bioscientia Medicina : Journal of Biomedicine and Translational Research Jurnal Ilmu Kesehatan Indonesia (JIKSI) Jurnal Otorinolaringologi Kepala dan Leher Indonesia (JOKLI) Jurnal Kesehatan Masyarakat Indonesia (JKMI) Jurnal Riset Ilmiah Seroja Husada: Jurnal Kesehatan Masyarakat Frontiers on Healthcare Research
Tofrizal
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Journal : Collaborative Medical Journal (CMJ)

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PENERAPAN TEKNIK PERIODIC ACID SCHIFF (PAS) PADA DIAGNOSIS HISTOPATOLOGI Fajriza Yona; Salmiah Agus; Tofrizal Tofrizal
Collaborative Medical Journal Vol 5 No 1 (2022): Januari
Publisher : LPPM Universitas Abdurrab

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Abstract

Histopathological tissue examination with routine staining sometimes cannot provide a clear morphology of certain cells or tissues, therefore special staining techniques based on histochemical reactions are needed. Periodic Acid-Schiff's (PAS) is a histochemical staining method specifically used to detect the presence of polysaccharide carbohydrates, neutral mucins and other glycoproteins. This article was written with the aim of increasing knowledge for practitionersor clinicians regarding the PAS technique procedure and how it is used in diagnosis. The method of writing scientific papers is a literature review sourced from various journals, books and scientific articles that are relevant and in accordance with the topics discussed. The PAS technique procedure begins with fixation in 10% neutral buffer formalin, preparation of materials and reagents, PAS staining with counterstain and interpretation. The addition of diastase was performed before PAS stain for PAS/D, and Alcian Blue staining was performed before PAS stain for the AB/PAS technique. Errors at each stage will affect the results of PAS staining. The use of combined PAS and PAS techniques in pathological diagnosis is to exclude the differential diagnosis of various tumors containing glycogen and mucin, examine the thickness of the basement membrane in various diseases, and identify Jamur and amoeba in tissue infections.
Co-Authors Abdiana Abdiana, Abdiana Afdal Afdal Afriani Afriani, Nita Aini, Julpa Nurul Aizah, Havina Nurul Aljassri, Resti Karunia Alvarino Alvarino Amel Yanis Aqilah, Giffary Zahida Ariani, Novita Arnofyan, Budi Pratama Asri, Ennesta Aswiyanti Asri Aswiyanti Asri Athika Rahmawati Avit Suchitra Azmal, Nita Afriani Cimi Ilmiawati, Cimi Daan Khambri Daan, Khambri Deddy Saputra Deddy Satriya Putra Desmawati Desmawati Dessy Arisanty Djong Hon Tjong Dolly Irfandy Dwi Yanti Fioni Putri Dwisari Dillasamola Elli Firdamila Elmatris Sy Endrinaldi Ennesta Asri Erkadius Erkadius Eryati Darwin Eryati Darwin Eti Yerizel Fachry Abda El Rahman Fadhilah, Maisarah Fadil Oenzil Fadila, Srigunda Arisya Fadillah Fadillah Fajriza Yona Fesdia Sari Firdamila, Elli Gardenia Akhyar Gusti Revilla Hafni Bachtiar Handriyani, Fitri Nur Hasmiwati henny Mulyani Henny Mulyani Hera Novianti Hirowati Ali, Hirowati Husna Yetti Husna, Yetti Intan, Shinta Ayu Kamelia, Muthia Khotimah, Rifqoh Kusumardani, Dini Lubis M Mahata, Liganda Endo Maliza, Rita Mayorita, Pamelia Miftah Irramah Muhammad Idris Muhammad Nazri Janra Mustika Sari Naqia, Masyithah Nita Afriani Nofrita Nofrita Novita Ariani Noza Hilbertina Noza Hilbertina, Noza Noza, Hilbertina Nur Afrinin Syah Nuzulia Irawati Perisnawati, Pemi Putra Santoso Putri Amran, Fajriana Anggun Putri, Biomechy Oktomalio Putri, Nabila Priscilla R. Zuryati Nizar Rahmadian, Rizki Rauza Sukma Rita Raymond, Benni Restu Susanti Ria Oktavia Ria Oktavia Rina Gustia Rio Hendra Robby Jannatan Rony Rustam Runky Pebranka Rusnita, Dewi RZ Nizar Safnita, Dewi Salmiah Agus Salmiah Agus Salmiah Agus Salmiah Agus Sari, Rini Purnama Selfi Renita Rusjdi, Selfi Selly Alinta Syukri Sisca Dwi Yarni Siti Nurhajjah Sri Lestari SRI LESTARI Sri Wahyuni Handayani Sri Wahyuni Handayani, Sri Wahyuni Suci Rahmadhani Sukri Rahman Syamel Muhammad Tiffany, Begum Utama, Bobby Indra Yarni, Sisca Dwi Yenita . Yenita Yenita Yenny Raflis Yerizal, Eti Yessy Setiawati Yessy Setiawati Zehan Afifa Yusran Zulfadli Syahrul, Muhammad