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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Biotropika Jurnal Al-Tadzkiyyah Journal of Tropical Biodiversity and Biotechnology Indonesian Journal of Chemistry Jurnal Analis Kesehatan Batoboh : Jurnal Pengabdian Pada Masyarakat Surya Abdimas Jurnal Biotek Cendekia Journal of Pharmacy JURNAL ANALIS LABORATORIUM MEDIK Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Ilmu dan Teknologi Kesehatan Indonesian Journal of Global Health research Lumbung Farmasi : Jurnal Ilmu Kefarmasian Sciscitatio : Journal for Biological Science Jurnal Farmasi Bioinformatics and Biomedical Research Journal Mestaka: Jurnal Pengabdian Kepada Masyarakat Journal of Biological Science and Education Indonesian Research Journal on Education Bengawan :Jurnal Pengabdian Masyarakat Journal of Research in Educational Management Rumphius Pattimura Biological Journal Media Kesehatan Politeknik Kesehatan Makassar Jurnal Pengabdian kepada Masyarakat Arumbai International Journal of Social and Education (INJOSEDU) Proceeding Of International Conference On Education, Society And Humanity Jurnal Pengabdian kepada Masyarakat Arumbai
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Hubungan Usia dan Status Menopause dengan Kejadian Bacterial Vaginosis pada Pasien Keputihan Budhi, Dono Setya; Wahyudi, Didik
Jurnal Analis Kesehatan Vol. 12 No. 1 (2023): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v12i1.3823

Abstract

Bacterial Vaginosis (BV) is a condition of imbalance in the vaginal ecosystem, namely decreased number Lactobacillus followed by an increase in numbers Gardnerella vaginalis and other anaerobic bacteria. About 90% of Indonesian women have the potential to experience vaginal discharge because Indonesia has a tropical climate and the incidence continues to increase every year up to 70%. The purpose of this research was to determine the correlation between age and menopausal status with the incidence bacterial vaginosis in vaginal patients. This research method is observational analytic, looking at the correlation between age and menopausal status with incidence bacterial vaginosis in vaginal patients. The number of samples used was 28 patients. Diagnosis bacterial vaginosis was carried out using the Gram staining method then a nugent scoring system was carried out by adding up the three morphological forms of bacteria found (large-sized Gram-positive rods, small-sized Gram-variable rods and bent Gram-negative rods/Gram-variable rods). Data analysis used fisher's exact test. Based on the results of the research, the prevalence of positive BV was 67.9% with the most characteristics of patients aged over 51 years at 90.9%, menopause at 90.9%. The fisher's exact test analysis showed a p-value for age = 0.020 (p
Deteksi Gen Resisten Kloramfenikol (cat) pada Isolat Klinik Pseudomonas aeruginosa dan Escherichia coli dengan Metode Polymerase Chain Reaction Wahyudi, Didik; Silviani, Yusianti; Nirwana, Ardy Prian; Saroh, Dewi
SCISCITATIO Vol. 5 No. 1 (2024): Volume 5 Number 1, January 2024
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2024.51.161

Abstract

Pseudomonas aeruginosa dan Escherichia coli adalah patogen oportunistik dari kelompok bakteri gram negatif yang menyebabkan infeksi pada tubuh manusia. Kedua spesies tersebut sering ditemukan telah resisten terhadap beberapa antibiotik. Kloramfenikol merupakan antibiotik yang sering digunakan untuk mengatasi infeksi bakteri tersebut, salah satu gen pengendali resistensi kloramfenikol adalah gen cat. Penelitian ini bertujuan untuk mendeteksi keberadaan gen resistensi kloramfenikol (cat) pada isolat klinik P. aeruginosa dan Escherichia coli. Bakteri diisolasi dari kasus infeksi di Rumah Sakit. Kemudian dilakukan identifikasi dengan metode uji biokimia, isolasi DNA, kemudian diamplifikasi dengan metode Polymerase Chain Reaction, dengan menggunakan dua pasang primer spesifik untuk kedua bakteri tersebut, dilanjutkan visualisasi hasil PCR dengan menggunakan elektroforesis. Hasil uji menunjukkan bahwa semua isolat P. aeruginosa terdeteksi adanya gen cat (100%), sedangkan E. coli terdeteksi gen cat sebesar 71,4%
Induction of Synthetic Polyploids of Porang (Amorphophallus muelerri Blume) and Assessment of Its Genetic Variability Using Morphological Data and RAPD Molecular Marker Suyono, Suyono; Indivia, Imey Tamara; Resmisari, Ruri Siti; Fitriyah, Fitriyah; Wahyudi, Didik
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.82238

Abstract

This study uses morphological characteristics and RAPD markers to evaluate the polyploidization of synthetic porang. Seeds of triploid porang (2n=2x=26) were soaked in the different colchicine concentrations for 24 hours. After colchicine treatment, the porang seeds were planted to an MS medium that contained 2.2 µM of 6-benzylaminopurine (BAP), then, 40 days after planting in the MS media, the morphology and molecular of synthetic polyploid porang were characterized. For DNA extraction, a total of 100 mg of young leaves of porang plantlet was collected. One way Anova followed by the Duncan test (95%) was performed for phenotypic characterization. The number of different alleles, number of effective alleles, Shannon's information index, diversity, and unbiased diversity were assessed for genetic diversity. Synthetic polyploid porang has a higher total shoot, root, and wider leaves than normal porang. Polyploidy induction also successfully increased the genetic diversity of porang, and the genetic diversity will increase porang adaptability and sustainability of porang cultivation. 
Variasi Genetik dan Filogeografi Pisang Raja (Musa spp.) di Pulau Jawa Berdasarkan Sekuen Internal Transcribed Spacer Wahyudi, Didik; Gusmiati, Lutfiana Hasanah; Hapsari, Lia
Jurnal Biotek Vol 11 No 2 (2023): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v11i2.40807

Abstract

Pisang Raja is an important banana cultivar for the Javanese people since ancient times, with many morphological variations and local names. This study aims to determine the genetic variation and phylogeography of Pisang Raja based on Internal Transcribed Spacer (ITS) sequences. The results of the research can be used as a reference for developing superior Pisang Raja cultivars that are resistant to disease and make farmers easier to choose Pisang Raja cultivars that suit with geographical conditions on Java Island. This study used 9 samples of Pisang Raja (in-group) and 2 samples of wild bananas (out-group). The research method started with DNA isolation, qualitative and quantitative tests, ITSL and ITS4 primer amplification, and sequencing. Data analysis performed included polymorphism, phylogenetics (MP, ML, NJ and BI) and haplotype. The results showed that the ITS sequences in Pisang Raja was amplified with a length of 600-700 bp and GC content of 61.70%. Polymorphism analysis showed that the genetic variation of Pisang Raja in Java was very high. Phylogenetic analysis resulted in 4 phylogenetic trees with relatively same topologies, where the in-groups were divided into 2 clades, namely clade I (AAA and AAB) and clade II (AAA and ABB). The highest genetic similarity was showed by Raja Brentel and Raja Bali (89.43%) and the least was Raja Gareng and Raja Seribu (56.82%). Haplotype analysis formed 11 haplotypes which grouped into 4 groups. Pisang Raja evolution comes from two ancestors, i.e. M. acuminata and M. balbisiana. The phylogeography of ITS gene flow in Pisang Raja originates from Central Java then spread to East Java and Jakarta. This is presumably due to the high utilization followed by the planting of Pisang Raja by the people of Central Java for ritual and ceremonial events in the past which influenced the domestication process. The results of this study can served as the reference for genetic conservation strategy of Pisang Raja.
Clitoria ternatea Increases Milk Production in Dairy Cows by Inhibiting Dopamine Receptor D2: A Computational Study Leondro, Henny; Hadiani, Dimas Pratidina Puriastuti; Krisnaningsih, Aju Tjatur Nugroho; Lestari, Oke Anandika; Wahyudi, Didik; Gusmalawati, Dwi; Tribudi, Yuli Arif; Prihandini, Peni Wahyu
Indonesian Journal of Chemistry Vol 24, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.92920

Abstract

Dairy cow's milk is a primary commodity in various countries and increasing milk production in dairy cows is crucial. Clitoria ternatea has the potential to enhance milk production in dairy cows. This research aims to analyze C. ternatea's ability to induce milk production in dairy cows by targeting the DRD2 protein. The compounds within C. ternatea were screened for drug-likeness, toxicity, physicochemical properties, and membrane permeability parameters. The DRD2 protein in dairy cattle was modeled using homology modeling. The interaction stability between C. ternatea compounds and DRD2 was analyzed through molecular docking and dynamic using AutoDock Vina and Webgro. The study results revealed that among the 18 compounds, 5 passed the drug-likeness screening: citronellal, alpha-terpinolene, 15-methyxypaysine, allyl-crotyl-zinc, and 9,12-octadecadiynoic. These 5 compounds exhibited low toxicity and demonstrated easy penetration of lipid membranes. Molecular docking results indicated that citronellal and alpha-terpinolene had the lowest binding energy values and were bound to the inhibitor's side. Molecular dynamic simulations also confirmed the stability of the interaction between citronellal and alpha-terpinolene with DRD2. In conclusion, this research suggests that C. ternatea can potentially increase milk production in dairy cows by inhibiting the DRD2 protein, primarily through citronellal and alpha-terpinolene.
ANALYSIS OF THE INFLUENCE OF FINANCIAL LITERACY ON PERSONAL FINANCIAL MANAGEMENT BEHAVIOR IN THE MILLENNIAL GENERATION Bambang Hermawan; Wahyudi, Didik
INTERNATIONAL JOURNAL OF SOCIAL AND EDUCATION Vol. 1 No. 6 (2024): September
Publisher : Pondok Pesantren Baitul Quran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research aims to analyze the influence of financial literacy on personal financial management behavior in the millennial generation. Using literature research methods, this study collects and analyzes various scientific sources to explain how financial knowledge and understanding contribute to the financial decisions taken by individuals in this age group. The research results show that financial literacy has a significant role in shaping millennial financial behavior. Millennials who have a high level of financial literacy are more likely to manage their income, expenses, savings and investments more effectively. They are also better able to avoid and manage debt wisely and plan long-term finances, including preparing emergency funds and retirement. In addition, good financial literacy can reduce the level of stress or anxiety related to finances among millennials, because they feel more confident in making smart financial decisions. This research underlines the importance of financial education programs that are enhanced and supported by various parties, including educational institutions, government and non-profit organizations, to increase financial literacy among the millennial generation. In conclusion, increasing financial literacy is a key strategy to improve personal financial management among millennials, which in turn can contribute to their financial stability and well-being. These findings are relevant for policy makers and practitioners working to support the development of the financial capacity of the millennial generation.
Pembuatan Video Profil Desa Sri Tanjung Kabupaten Mesuji Lampung Sebagai Media Informasi dan Promosi Amriyah, Chairul; Witjaksono, M Dimas Yudi; sari, Sonya Permata; Wahyudi, Didik
Batoboh Vol 9, No 2 (2024): BATOBOH: JURNAL PENGABDIAN PADA MASYARAKAT
Publisher : Institut Seni Indonesia Padangpanjang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26887/bt.v9i2.4782

Abstract

Tujuan pengabdian ini adalah untuk mengenalkan informasi dan promosi dari potensi-potensi desa Sri Tanjung melalui pembuatan video profil desa, dengan memanfaatkan teknologi digital. Kegiatan pengabdian masyarakat ini berada di desa Sri Tanjung, Kecamatan Tanjung Raya, Kabupaten Mesuji, Lampung, desa yang berbatasan langsung dengan Provinsi Sumatera Selatan. Desa Sri Tanjung memiliki beragam potensi yang dapat dimanfaatkan dan dikembangkan, beberapa diantaranya, perikanan, sentra kerupuk ikan, kemplang, dan kue semprong. Metode pengabdian ini menggunakan Community Based Research (CBR), metode ini relevan karena mahasiswa dapat mengembangkan ide dan bekerja sama dengan masyarakat untuk mengatasi masalah yang di hadapi. Hasil dari pembuatan video ini dapat memberikan dampak kesadaran publik terhadap potensi desa, dengan manampilkan produk unggulan dan budaya lokal desa Sri Tanjung melalui penyampaian visual yang dikemas dengan narasi menarik.
Penyuluhan dan Edukasi Senam Ergonomik untuk Mengatasi Masalah Asam Urat pada Lansia di Desa Cemani, Kecamatan Grogol Kabupaten Sukoharjo Wahyudi, Didik; Widyastuti, Endang; Nawang, Pungky; Hidayatullah, Rafi Fuzan; Septiani, Risma Dyah; Purwandani, Safitri; Putri, Sheli Feliyan
Jurnal Pengabdian Arumbai Vol 2 No 2 (2024)
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/arumbai.vol2.iss2.pp213-218

Abstract

High uric acid levels cause pain and swelling in the bones and joints, which are commonly experienced by most elderly individuals. Another factor that can increase uric acid levels is the excessive consumption of foods high in purines. Non-pharmacological treatment can be used to control uric acid levels, one of which is by performing ergonomic exercises. Ergonomic exercises involve muscle movements combined with breathing techniques. The purpose of this community service program is to enhance the elderly's knowledge of managing uric acid levels through ergonomic exercise training. The activities include delivering educational material, conducting ergonomic exercise training, as well as discussions and Q&A sessions. The results of this community service activity indicate that the elderly understood the material presented. Through presentations and ergonomic exercise demonstrations, there was a 22% increase in understanding, and they were able to perform ergonomic exercises independently.
Penyuluhan Tentang Bahaya Merokok dan Sulitnya Berhenti Merokok Dengan Pendekatan Teori Kognitif Trikusumaadi, Stefanus Khrismasagung; Wahyudi, Didik
Bengawan : Jurnal Pengabdian Masyarakat Vol 4 No 2 (2024): Desember
Publisher : Politeknik Indonusa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46808/jurnal_bengawan.v4i2.157

Abstract

Issue seputar merokok menjadi salah satu topik yang diperdebatkan dari waktu ke waktu. Upaya menurunkan angka konsumsi rokok menjadi salah satu agenda pemerintah setiap tahunnya. Meskipun telah menggunakan banyak peringatan melalui berbagai pihak, namun masih tetap berhadapan pada permasalahan sulitnya berhenti merokok. Hal itu tidak terlepas dari berbagai faktor yang mempengaruhi perilaku merokok, seperti faktor sosial dan budaya. Untuk itu penyampaian tentang teori disonansi kognitif pada perilaku merokok patut dipaparkan sebagai bagian dari penyuluhan kesehatan tentang bahaya rokok. Kegiatan penyuluhan dilakukan terhadap oleh karangtaruna desa Kingkang wonosari, klaten. Hasil penyampaian materi mampu meningkatkan nilai rata-rata pengetahuan peserta sebesar 3,92 dan nilai rata-rata sikap peserta sebesar 0,32. Pengetahuan yang relatif baru bagi peserta adalah tentang disonansi kognitif. The issue of smoking is one of most debatable topic from time to time. Efforts to reduce cigarette consumption become one of the government's agenda every year. Although they have used many warnings through various parties, they are still faced with the problem of the difficulty of quitting smoking. This is inseparable from various factors that influence smoking behavior, such as social and cultural factors. For this reason, the presentation of cognitive dissonance theory on smoking behavior should be presented as part of health education about the dangers of smoking.Counseling activities were carried out by the youth organization in Kingkang village, Wonosari, Klaten. The results of the delivery of the material were able to increase the average value of participants' knowledge by 3.92 and the average value of participants' attitudes by 0.32. Knowledge that was relatively new to participants was about cognitive dissonance.
Deteksi Gen SLC30A8 pada Sampel Darah Penderita Diabetes Melitus Tipe 2 Dengan Metode Polymerase Chain Reaction Auliantika, Dinda Helsa; Wahyudi, Didik
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 9 No 2 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol9issue2page255-262

Abstract

Background: The SLC30A8 gene is a zinc efflux transporter associated with insulin secretion expressed by the pancreas. The SLC30A8 gene causes abnormalities in insulin synthesis, maturation & secretion as well as abnormalities in the body's effectiveness in responding to glucose metabolism. Polymerase Chain Reaction (PCR) is a DNA amplification method that can be used to detect the presence of the SLC30A8 gene in type 2 diabetes mellitus patients. Methods: This study used a descriptive research method conducted at the Molecular Biology Laboratory of Sekolah Tinggi Ilmu Kesehatan Nasional. The sampling technique in this study used a total sampling technique on 25 type 2 diabetes mellitus patients who were members of the Prolanis Diabetes Mellitus Puskesmas Bulu Sukoharjo, Central Java. The detection of the SLC30A8 gene went through several stages, namely DNA isolation, qualitative testing of DNA isolates, quantitative testing of DNA isolates and PCR Results: The results showed the presence of the SLC30A8 gene in all blood samples from type 2 diabetes mellitus patients using the PCR method. The primer used with forward primer 5'-GGACGAAAGAGTTCCCATAGCG-3' and reverse primer 5'- ATAGCAGCATGTTTTGAAGGTGGC-3' attaches specifically to chromosome 8 with a product length of 429 bp. Conclusion: All blood samples of type 2 diabetes mellitus patients detected the SLC30A8 gene
Co-Authors Afianti, Linda Dwi Afiati, Lely Agung, Rendyansah ahmad yani Aju Tjatur Nugroho Krisnaningsih Alfaruqi, Nuri Thobibatus Shofia Anak Agung Istri Sri Wiadnyani Andriani, Disa Anjarani, Ajeng Viona Putri Annisa, Nenden Nur Anwar, Muhamad Syaeful Ardani, Edvi Gracia Ardy Prian Nirwana, Ardy Prian Auliantika, Dinda Helsa Bambang Hermawan Bayyinatul Muchtaromah Budhi, Dono Setya Chairul Amriyah Dian Puspita Sari Dwi Gusmalawati Endang Sutariningsih Soetarto Endang Widyastuti Ervin Medianti Fadhila, Zulfa Nur Fitriyah Fitriyah Gusmiati, Lutfiana Hasanah Hadiani, Dimas Pratidina Puriastuti Hasan, M. Nur Hidayatullah, Rafi Fuzan Ilmi, Zahrobatul Lil Indivia, Imey Tamara Isna Arofatun Nikmah, Isna Arofatun Istiqomah, Safitri Nur Juwanita, Agnes Kharisma, Viol Dhea Kusuma, Eka Wisnu Leondro, Henny Lia Hapsari M. Dimas Yudi Witjaksono Manshur, umar Mauludyah, Rohmatul Mimi Salmawati Mose, Windi Muhammad Saefi Mujahidin Ahmad Munandar, Hanozanor Fadli Nawang, Pungky Oke Anandika Lestari Pratimasari, Diah Prihandini, Peni Wahyu PUDJI ASTUTI Purwandani, Safitri Putri, Sheli Feliyan Rahmawati, Valentina Indah Rodiyati Azrianingsih Ruri Siti Resmisari Rusdi, Nurhidaya Saputra, Dedech Muhammad Sari, Retno Komala sari, Sonya Permata Saroh, Dewi Septiadi, Luhur SEPTIANA SEPTIANA Septiani, Risma Dyah Setya, Adhi Kumoro Setyazi, Gama Stefanus Khrismasagung Trikusumaadi, Stefanus Khrismasagung Susilowati Susilowati SUYONO Suyono Suyono Syafe’I, Imam Syafrudin Syafrudin Syahran Wael Umi Fathonah, Yuliana Valent, Febriana Verawati, Liesta Wasehudin, Wasehudin Widyastuti, Dinda Yuli Arif Tribudi Yusianti Silviani