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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Biotropika Jurnal Al-Tadzkiyyah Journal of Tropical Biodiversity and Biotechnology Indonesian Journal of Chemistry Jurnal Analis Kesehatan Batoboh : Jurnal Pengabdian Pada Masyarakat Surya Abdimas Jurnal Biotek Cendekia Journal of Pharmacy JURNAL ANALIS LABORATORIUM MEDIK Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Ilmu dan Teknologi Kesehatan Indonesian Journal of Global Health research Lumbung Farmasi : Jurnal Ilmu Kefarmasian Sciscitatio : Journal for Biological Science Jurnal Farmasi Bioinformatics and Biomedical Research Journal Mestaka: Jurnal Pengabdian Kepada Masyarakat Journal of Biological Science and Education Indonesian Research Journal on Education Bengawan :Jurnal Pengabdian Masyarakat Journal of Research in Educational Management Rumphius Pattimura Biological Journal Media Kesehatan Politeknik Kesehatan Makassar Jurnal Pengabdian kepada Masyarakat Arumbai International Journal of Social and Education (INJOSEDU) Proceeding Of International Conference On Education, Society And Humanity Jurnal Pengabdian kepada Masyarakat Arumbai
Didik Wahyudi
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Penyuluhan dan Edukasi Senam Ergonomik untuk Mengatasi Masalah Asam Urat pada Lansia di Desa Cemani, Kecamatan Grogol Kabupaten Sukoharjo Wahyudi, Didik; Widyastuti, Endang; Nawang, Pungky; Hidayatullah, Rafi Fuzan; Septiani, Risma Dyah; Purwandani, Safitri; Putri, Sheli Feliyan
Jurnal Pengabdian Arumbai Vol 2 No 2 (2024)
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/arumbai.vol2.iss2.pp213-218

Abstract

High uric acid levels cause pain and swelling in the bones and joints, which are commonly experienced by most elderly individuals. Another factor that can increase uric acid levels is the excessive consumption of foods high in purines. Non-pharmacological treatment can be used to control uric acid levels, one of which is by performing ergonomic exercises. Ergonomic exercises involve muscle movements combined with breathing techniques. The purpose of this community service program is to enhance the elderly's knowledge of managing uric acid levels through ergonomic exercise training. The activities include delivering educational material, conducting ergonomic exercise training, as well as discussions and Q&A sessions. The results of this community service activity indicate that the elderly understood the material presented. Through presentations and ergonomic exercise demonstrations, there was a 22% increase in understanding, and they were able to perform ergonomic exercises independently.
Pengaruh Ekstrak Alpinia galanga L Terhadap Produksi Biofilm pada Escherichia coli Wahyudi, Didik; Wael, Syahran
Jurnal Farmasi (Journal of Pharmacy) Vol. 10 No. 1 (2021): Jurnal Farmasi (Journal of Pharmacy), April 2021
Publisher : Jurnal Farmasi (Journal of Pharmacy)

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Abstract

Escherichia coli is a Gram-negative rod-shaped bacteria, capable of causing infection in several parts of the body, and has been found to be resistant to various antibiotics, one of the contributing factors is its ability to form biofilms in tissues. Alpinia gallanga L has the ability to inhibit biofilm formation against several bacteria. The purpose of this study was to determine the ability of Alpinia galangl L extract in inhibiting the production of Escherichia coli biofilm. This research began with the extraction of galangal rhizome with ethanol using the masersion method, then made the concentrations of 10%, 20%, 30%, 40%, and 50%. Escherichia coli was isolated from cases of Urinary Tract Infection (UTI). then do the physiological characterization and sensitivity test to antibiotics. Inhibition test for Escherichia coli biofilm was carried out using the microtiter plate culture method using a spectrophotometer at a wavelength of 595nm. The measurement results of biofilm production were 0.1% Optical Density crystal violet value, each treatment used 8 replications, the data obtained were analyzed by One Way Anova. . The results showed that the extract of Alpinia galanga L was able to inhibit the production of Escherichia coli biofilm at a concentration of 30%.
Pembentukan Biofilm Pseudomonas aeruginosa pada Beberapa Media Cair Wahyudi, Didik; Sutariningsih Soetarto, Endang
Jurnal Farmasi (Journal of Pharmacy) Vol. 10 No. 2 (2021): Jurnal Farmasi (Journal of Pharmacy), October 2021
Publisher : Jurnal Farmasi (Journal of Pharmacy)

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Abstract

Pseudomonas aeruginosa is a Gram-negative rod-shaped bacterium that is an opportunistic pathogen that is the main cause of nosocomial infections and is able to form biofilms on growth media, biofilms often make the treatment of infectious diseases more difficult. There are several types of bacterial growth media, compositions and brands. The purpose of this study was to determine the ability of P. aeruginosa to form biofilms on several liquid culture media. P. aeruginosa was isolated from clinical samples from hospitals, the liquid media used were nutrient broth, lactose broth, brain-heart infusion (BHI), luria bertani broth, and tripticase soy broth. The biofilm formation test used the microtiter plate culture technique method, the ability to form biofilms was measured based on optical density using a microtiter plate reader at a wavelength of 570nm, with 0.1% crystal violet staining, after 24 hours incubation at 37oC, with 8 replications. The results showed that P. aeruginosa had the ability to form biofilms in nutrient broth 0.926±0.081, lactose broth 0.521±0.041, BHI 1.283±0.031, luria bertani 1,301±0.043, and trypticase soy broth media 1.563±0.032. The highest biofilm formation was in trypticase soy broth, and the lowest was in lactose broth, while in BHI and luria farming media the biofilm formation abilities were equivalent. The conclusion of this study is that P. aeruginosa has different abilities in forming biofilms when grown on different liquid media.
Uji Aktivitas Antibakteri Ekstrak Etanol dan Fraksi Etil Asetat Beras Hitam (Oryza Sativa L.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli: Jurnal Farmasi (Journal of Pharmacy), October 2025 Umi Fathonah, Yuliana; Wahyudi, Didik; Pratimasari, Diah
Jurnal Farmasi (Journal of Pharmacy) Vol. 14 No. 2 (2025): Jurnal Farmasi (Journal of Pharmacy), October 2025
Publisher : Sekolah Tinggi Ilmu Kesehatan Nasional & Pengurus Cabang Ikatan Apoteker Indonesia (IAI) Sukoharjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.373013/adx0af97

Abstract

Beras hitam (Oryza Sativa L.) merupakan beras lokal yang memproduksi antosianin dengan intesitas tinggi pada aleuron dan endospremia sehingga warna beras menjadi ungu pekat menjadi hitam. Beras hitam memiliki kandungan kimia flavonoid, tanin, dan alkoloid. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol dan fraksi etil asetat beras hitam (Oryza Sativa L.) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Serbuk beras hitam dimaserasi menggunakan etanol 96%, kemudian difraksinasi menggunakan pelarut etil asetat. Pengujian aktivitas antibakteri ekstrak etanol dan fraksi etil asetat dilakukan dengan menggunakan metode difusi cakram. Pengujian metode difusi dengan menggunakan konsentrasi 25%,30%, dan 35% menggunakan kontrol positif Ciprofloxacin 5µg terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil pengujian aktivitas antibakteri menunjukkan ekstrak etanol dan fraksi etil asetat beras hitam dapat menghambat pertumbuhan bakteri dengan adanya daya hambat. Fraksi etil asetat konsentrasi 35% merupakan fraksi teraktif dengan rata-rata diameter zona hambat sebesar 17,47 mm pada bakteri Staphylococcus aureus sedangkan pada bakteri Escherichia coli rata-rata diameter zona hambat fraksi etil asetat sebesar 14,52 mm. Berdasarkan uji analisis data one way anova, masing-masing konsentrasi ekstrak etanol dan fraksi etil asetat menunjukkan adanya perbedaan bermakna dalam aktivitas antibakteri dan tidak memiliki kemampuan aktivitas antibakteri setara dengan kontrol positif Ciprofloxacin dengan nilai sig.(P<0.05).
Implementasi Pendidikan Agama: Pembelajaran Pendidikan Agama Bagi Peserta Didik Beda Agama Wahyudi, Didik
Indonesian Research Journal on Education Vol. 4 No. 2 (2024): irje 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/irje.v4i2.562

Abstract

Penyelenggaraan pendidikan agama pada lembaga pendidikan bagi siswa yang tidak seagama di Kota Metro tidak dapat berjalan sebagaimana mestinya, oleh karena itu perlu kerja sama semua pihak untuk melakukan perbaikan, pengawasan yang dilakukan dari Dinas Pendidikan Kemenag Kota Metro, Pengawas dan Kepala Sekolah, termasuk di dalamnya adalah masyarakat. Undang-Undang Nomor 20 Tahun 2003 Bab V Pasal 12 Peraturan Pemerintah Nomor 55 Tahun 2007, harus dapat diterapkan dengan baik pada semua lembaga khususnya penyelenggara pendidikan. Sebab hingga saat ini belum terlihat sanksi tegas yang diberikan kepada lembaga pendidikan yang tidak menjalankan amanah aturan tersebut. Apalagi sanksi tegas hingga penutupan suatu lembaga pendidikan. Dengan demikian dapat dikatakan bahwa ketentuan yang diatur dalam Undang-Undang Nomor 20 Tahun 2003 tentang Bab V Pasal 12 tidak dilaksanakan dengan baik oleh penyelenggara pendidikan di sekolah, khususnya pada tingkat SMA Kota Metro. Dalam penelitian ini permasalahan yang muncul; Yang pertama : tidak diberikannya pendidikan agama bagi siswa muslim menuai kekhawatiran orang tua wali oleh karena itu meminta Pemerintah Daerah untuk membatalkan kasus tersebut, yang kedua: SMA Kristen Metro tidak menyelenggarakan pendidikan Islam di sekolah, alasannya bagi siswa yang beragama Islam sedikit agar pihak sekolah tidak memperbantukan guru-guru yang mengajarkan agama islam dan hindu, yang ketiga : ada peraturan yang tidak memenuhi standar pendidikan, pengawas disatuan pendidikan harus bisa turun tangan mencari solusi dengan menempatkan guru agama islam dan hindu di lembaga tersebut.
FORMULASI NANOPARTIKEL FRAKSI ETIL ASETAT EKSTRAK ETANOL BUNGA TELANG (Clitoria ternatea L.) dan UJI ANTIBAKTERI Staphylococcus aureus YANG DIISOLASI DARI JERAWAT Fadhila, Zulfa Nur; Andriani, Disa; Wahyudi, Didik
Cendekia Journal of Pharmacy Vol 8, No 2 (2024): Cendekia Journal of Pharmacy
Publisher : Institut Teknologi Kesehatan Cendekia Utama Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31596/cjp.v8i2.295

Abstract

Jerawat merupakan penyakit peradangan kronik kelenjar pilosebasea dengan meningkatnya produksi sebum, peluruhan keratinosit, tumbuh bakteri dan inflamasi. Bahan alam terbukti bermanfaat bagi kesehatan dan efek samping yang jauh lebih rendah jika dibandingkan dengan obat sintesis. Salah satu tanaman yang memiliki aktivitas antibakteri yaitu bunga telang (Clitoria ternatea L.). Efisiensi penggunaan bahan alam dapat ditingkatkan kemampuan fungsi obat jerawat dengan membuat sediaan dalam bentuk nanopartikel. Penelitian ini bertujuan untuk mengetahui pengaruh kitosan terhadap karakteristik fisik nanopartikel fraksi etil asetat ekstrak etanol bunga telang dan aktivitas antibakterinya terhadap Staphylococcus aureus. Metode ekstraksi yang digunakan adalah maserasi dengan menggunakan etanol 70%. Pembuatan nanopartikel menggunakan metode gelasi ionik dengan polimer kitosan dan natrium tripolifosfat dengan variasi konsentrasi kitosan (0,1%, 0,2%, 0,3%). Pengujian aktivitas antibakteri terhadap Staphylococcus aureus dengan metode sumuran. Data dianalisis dengan One Way ANOVA yang dilanjutkan dengan uji Post Hoc. Hasil karakteristik nanopartikel F1, F2 dan F3 menunjukkan ukuran partikel 69,58±6,90 nm, 166,47±37,06 nm, 539,63±105,40 nm dan zeta potensial +46,5±0,5 mV, +44,1±1,1 mV dan +37,7±0,8 mV. Hasil uji aktivitas antibakteri Formula 1, Formula 2 dan Formula 3 menunjukkan diameter zona hambat yaitu 11,38±0,71 mm, 11,82±0,81 mm dan 13,14±0,66 mm. Semua formula memenuhi sifat fisik nanopartikel dan memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus. 
Phytochemical Analysis, FTIR Spectroscopic of Flavonoid and Antibacterial Activity of Crassocephlum Crepidiodes (Benth.) S. Moore Leaf and Herbs Extract Sari, Retno Komala; Susilowati, Susilowati; Wahyudi, Didik
Indonesian Journal of Global Health Research Vol 6 No S5 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6iS5.4540

Abstract

Staphylococcus aureus is a bacteria that causes various skin diseases. C. crepidiodes contains flavonoid compounds which are thought to be responsible for antibacterial activity. This research aims to identify flavonoid compounds and antibacterial activity in the leaves (LC) and herbs (HC) of C. crepidiodes. LC and HC were extracted with 70% ethanol using the maceration method. The secondary metabolites were evaluated by phytochemical analysis and flavonoid compound identification using the Spectrophotometer Fourier Transform Infra-Red (FTIR) test and the antibacterial activity was conducted by the Well method against S. Aureus. The results showed that the secondary metabolite compounds of LC were different from HC and the differences FTIR spectra in transmittance peaks in the identified functional group hydroxyl stretching vibrations correlate with the antibacterial activity of S. Aureus. The antibacterial activity test of C. crepidiodes leaf extract had an average inhibitory zone concentration of 8.35mm to 15.09mm. In comparison, herbal extracts had an average inhibitory zone of 13.61mm to 18.11mm. HC (average inhibitory zone of 13.61mm to 18.11mm) has greater antibacterial activity than LC (average inhibitory zone concentration of 8.35mm to 15.09mm). The herbs parts of C. crepidiodes can be recommended as active antibacterial natural ingredients. This understanding is important to improve the quality of traditional medicine raw materials.
Karakterisasi Hidrolisat Keratin Bulu Ayam Menggunakan Ekstraksi Enzim Kasar Keratinase Dari Bakteri Bacillus thuringiensis Wahyudi, Didik; Saputra, Dedech Muhammad; Sari, Dian Puspita
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 5, No 1 (2024): Januari
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v5i1.17498

Abstract

Keratin merupakan produk pengerasan jaringan epidermal dari tubuh dan merupakan protein fibrous yang kaya akan sulfur dan merupakan polimer ketiga yang paling melimpah di lingkungan setelah selulosa dan kitin. Bulu ayam dapat digunakan sebagaai bahan alternatif keratin karena belum terolah optimal, namun memiliki kandungan utamanya berupa protein keratin ± 90%. Enzim keratinase mampu menghidrolisis berbagai protein larut dan protein tidak larut seperti protein keratin. Penelitian ini untuk mengetahui hasil hidrolisat keratin dari enzim kasar keratinase dari bakteri Bacillus thuringiensis dan uji fisika kimia hidrolisat keratin. Metode pembuatan enzim kasar keratinase dilakukan fermentasi bulu ayam menggunakan Bacillus thuringiensis. Hidrolisat keratin bulu ayam diperoleh dari enzim kasar keratinase menggunakan Bacillus thuringiensis yang dilakukan karakterisasi uji fisika dan kimia. Hasil aktivitas enzim keratinase sebesar 3,926 unit/ml yang menghasilkan rendemen 27,2 % Perlakukan ekstraksi dengan enzim keratinase menggunakan Bacillus thuringiens menunjukan hasil yang memenuhi syarat yaitu uji ninhidrin dengan warna kebiruan, uji organoleptis, uji kadar air, dan uji pH sedangkan pada uji rendemen, uji kadar abu, dan uji kadar protein tidak memenuhi. Uji FTIR diperoleh hasil pada bilangan serapan 923,94 cm-1, 1232,57 cm-1, 1527,69 cm-1, 1656,92 cm-1, 3296,49 cm-1
Phenotypic Variation and RAPD Polymorphism of Pisang Kepok Local Cultivars (Musa acuminata x Musa balbisiana, ABB, Saba Subgroup) Wahyudi, Didik; Ilmi, Zahrobatul Lil; Hapsari, Lia
Biotropika: Journal of Tropical Biology Vol. 10 No. 3 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.03.03

Abstract

Pisang Kepok is a major local banana cultivar in Indonesia with high economic, social and cultural value. Particularly on the island of Java, there are several variations of Pisang Kepok recognised with their own local names, which makes difficulties in taxonomic identification and grouping. Morphological features are used in conventional banana cultivar classification, but they are deemed less precise due to their subjectivity, thus, it is supposed to be complemented with a molecular approach. This study aims to identify the phenotypic variation of Pisang Kepok local cultivars also their genetic polymorphism using Random Amplified Polymorphic DNA marker. Phenotypic variation was observed using 35 morphological characters. Six RAPD primers were used, i.e. OPA2, OPA3, OPA4, OPA11, OPA12, and OPA18. Clustering analysis, both phenotypic and genetic were performed using PAST v4.02. The morphological characterisation identified four variants of Pisang Kepok i.e. Kepok Abang, Kepok Putih, Kepok Manurun, and Kepok Australi; which all confirmed as ABB genome group. Phenotypic clustering showed that Pisang Kepok cultivars were separated into 3 clusters based on their local name, with a high similarity value of >90%. PCA biplot showed that the fruit flesh colour was the most important character contributed to the cultivar variation. RAPD marker also showed that each specimen was grouped according to its local name and source, with a similarity value of >80%. Both morphology and molecular (RAPD) markers resulted in the branching of Pisang Kepok which was closer to Pisang Klutuk than Pisang Barlin.
Identifikasi dan Uji Sensitivitas Staphylococcus sp. Terhadap Beberapa Antibiotik pada Ulkus Diabetikum Septiana, Septiana; Anjarani, Ajeng Viona Putri; Wahyudi, Didik
Media Kesehatan Politeknik Kesehatan Makassar Vol 19 No 1 (2024): Media Kesehatan
Publisher : Direktorat Politeknik Kesehatan Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/medkes.v19i1.571

Abstract

Staphylococcus sp bacteria can cause infection in diabetic ulcers. Treatment of infections can use antibiotics, but the use of antibiotics can have negative impacts such as bacterial resistance to antibiotics. The aim of this research is to determine the results of identifying the bacteria Staphylococcus sp. (Staphylococcus aureus and Staphylococcus epidermidis) in diabetic ulcers and bacterial sensitivity to the antibiotics gentamicin, clindamycin, chloramphenicol and ciprofloxacin. The research design in this study used descriptive observational. The samples in this study were taken by accidental sampling in the period November to December 2023, resulting in 10 samples. Bacterial identification was carried out using biochemical test methods and sensitivity tests using the Kirby-Bauer method. The results of the bacterial identification research found Staphylococcus aureus bacteria in 7 out of 10 samples and Staphylococcus epidermidis bacteria in 3 out of 10 samples. The results of the sensitivity test concluded that the bacteria Staphylococcus sp. sensitive to the antibiotics gentamicin by 70%, clindamycin by 50%, chloramphenicol by 70% and ciprofloxacin by 70%.
Co-Authors Afianti, Linda Dwi Afiati, Lely Agung, Rendyansah ahmad yani Aju Tjatur Nugroho Krisnaningsih Alfaruqi, Nuri Thobibatus Shofia Anak Agung Istri Sri Wiadnyani Andriani, Disa Anjarani, Ajeng Viona Putri Annisa, Nenden Nur Anwar, Muhamad Syaeful Ardani, Edvi Gracia Ardy Prian Nirwana, Ardy Prian Auliantika, Dinda Helsa Bayyinatul Muchtaromah Budhi, Dono Setya Chairul Amriyah Dian Puspita Sari Dwi Gusmalawati Endang Sutariningsih Soetarto Endang Widyastuti Ervin Medianti Fadhila, Zulfa Nur Fitriyah Fitriyah Gusmiati, Lutfiana Hasanah Hadiani, Dimas Pratidina Puriastuti Hasan, M. Nur Hidayatullah, Rafi Fuzan Ilmi, Zahrobatul Lil Indivia, Imey Tamara Isna Arofatun Nikmah, Isna Arofatun Istiqomah, Safitri Nur Juwanita, Agnes Kharisma, Viol Dhea Kusuma, Eka Wisnu Leondro, Henny Lia Hapsari M. Dimas Yudi Witjaksono Manshur, umar Mauludyah, Rohmatul Mimi Salmawati Mose, Windi Muhammad Saefi Mujahidin Ahmad Munandar, Hanozanor Fadli Nawang, Pungky Oke Anandika Lestari Pratimasari, Diah Prihandini, Peni Wahyu PUDJI ASTUTI Purwandani, Safitri Putri, Sheli Feliyan Rahmawati, Valentina Indah Rodiyati Azrianingsih Ruri Siti Resmisari Rusdi, Nurhidaya Saputra, Dedech Muhammad Sari, Retno Komala sari, Sonya Permata Saroh, Dewi Septiadi, Luhur SEPTIANA SEPTIANA Septiani, Risma Dyah Setya, Adhi Kumoro Setyazi, Gama Stefanus Khrismasagung Trikusumaadi, Stefanus Khrismasagung Susilowati Susilowati SUYONO Suyono Suyono Syafe’I, Imam Syafrudin Syafrudin Syahran Wael Umi Fathonah, Yuliana Valent, Febriana Verawati, Liesta Wasehudin, Wasehudin Widyastuti, Dinda Yuli Arif Tribudi Yusianti Silviani