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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian Agroland : Jurnal Ilmu-Ilmu Pertanian Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology International Journal of Renewable Energy Development Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Berkala Ilmiah Pertanian Jurnal Agro Teknologi Tropika Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Sains Tanah AGRIVITA, Journal of Agricultural Science agriTECH Indonesian Journal of Biotechnology Jurnal Tanah dan Air Planta Tropika Pharmacon Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Perlindungan Tanaman Indonesia INDONESIAN JOURNAL OF PHARMACY JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Caraka Tani: Journal of Sustainable Agriculture Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Food and Nutrition Progress OLDI (Oseanologi dan Limnologi di Indonesia) JURNAL PERTANIAN jurnal Ilmu Pertanian (Agricultural Science) Jurnal Tanah dan Air (Soil and Water Journal) Kaunia: Integration and Interconnection of Islam and Science Journal Engagement: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Pharmacology and Therapy

JAKA WIDADA

Departemen Mikrobiologi Pertanian, Fakultas Pertanian, Universitas Gadjah Mada

Author-ID : 414939

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary

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Pengaruh Perbedaan Metode Ekstraksi Metabolit Sekunder Streptomyces sp. GMR22 terhadap Toksisitas pada Sel BHK-21 Mentari, Diani; Naima, Mirtani; Wulansari, Riska; Widada, Jaka; Nuringtyas, Tri Rini; Wibawa, Tri; Wijayanti, Nastiti
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 1 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i1.8032

Streptomyces sp. GMR22 is local isolate from Wanagama 1 Forest in Yogyakarta. They have the potential to be developed to produce active compounds because have PKS and NRPS genes.The active compounds from isolation are strongly influenced by various factors, one of them is extraction techniques. Effect difference of extraction technique will be affected by the quality of secondary metabolites produced.The purpose of this study was to compare the cytotoxicity effects of secondary metabolites of Streptomyces sp. GMR22 which have extracted with different stages from previous studies. The extraction technique was carried out by multilevel separatory funnel extraction methods, which was first extracted using non-polar solvent (n-hexane) and then extracted using semi-polar solvent (ethyl acetate). This research is important because in previous studies (separatory funnel only extracted using ethyl acetate) with the use of the lowest concentration in the dengue virus antiviral test (further test) caused 100% of deaths in BHK-21 cells.This study indicate that multilevel extraction result in lower CC50 value than previous studies. There are 49.160 µl/ml (n-hexane extract) and 284.56 µl/ml (ethyl acetate extract) while water extract is 464,38 µl/ml. FTIR compound analysis show that the three extracts produced have different spectrum patterns, especially in the n-hexane and ethyl acetate extract. Value of CC50 is not too high, it is expected that the secondary metabolites contained in the extracts can be used for further analysis such as antiviral testing because it is safe for normal host cells such as BHK-21 cells

Sebaran Penyakit Hawar Daun Bakteri di Beberapa Sentra Produksi Bawang Merah di Indonesia Asrul, Asrul; Arwiyanto, Triwidodo; Hadisutrisno, Bambang; Widada, Jaka
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 18, No 1 (2013): February 2013
Publisher : Universitas Atma Jaya Yogyakarta

Penelitian ini bertujuan mengetahui daerah sebaran penyakit hawar daun bakteri di beberapa sentra pertanaman bawang merah di Indonesia dan kultivar bawang merah yang dapat diinfeksi, serta mengidentifikasi patogen penyebabnya. Penentuan lokasi pengamatan dan pengambilan sampel dilakukan secara stratified purpossive random sampling. Survei dilakukan dengan cara wawancara dan pengamatan di lapangan (observasi) terhadap kultivar bawang dan gejala penyakit yang terinfeksi oleh bakteri patogen. Sampel diidentifikasi melalui pengamatan morfologi koloni, uji postulat Koch, uji reaksi hipersensitif dan pengujian sifat-sifat biokimia dan fisiologi. Hasil penelitian menunjukkan bahwa penyakit hawar daun bakteri telah tersebar secara merata di seluruh daerah pertanaman bawang merah di Indonesia, yang meliputi Kabupaten Cirebon, Tegal, Nganjuk, Bantul, dan Sigi, dengan tingkat serangan mencapai 62,5â€“100%. Penyakit ini menginfeksi bawang merah kultivar Bima curut, Bauji, Biru-sawah, dan Palasa. Gejala hawar daun bakteri yang dijumpai berupa water soaking, terjadi lekukan daun, pengerutan daun,Â klorosis, nekrosis, mati pucuk, pertumbuhan kerdil, dan kematian. Isolat bakteri yang ditemukan mempunyai bentuk koloni bulat, cembung, berlendir, dan berwarna kuning. Ciri morfologi koloni, gejala dan karakteristik isolat bakteri mirip dengan sifat-sifat bakteri Xanthomonas axonopodis pv. allii penyebab penyakit hawar daun pada bawang bombay.Kata kunci: Sebaran, bawang merah hawar daun bakteri, Xanthomonas axonopodis pv. allii

FITOREMEDIASI KANDUNGAN KROMIUM PADA LIMBAH CAIR MENGGUNAKAN TANAMAN AIR Safarrida, Anna; ., Ngadiman; Widada, Jaka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Existence of heavy metals in industrial waste is gaining global attention since their negative impact to environment. One of the efforts to solve the problem was to use plant to absorb metal in liquid medium, known as rhizofiltration. This research was aimed to select aquatic plantsÃ‚Â which showed relative resistantce and susceptibility to chromium. Four species of aquatic plants (Pistia stratiotes, Eichhornia crassipes, Lemna minor and Salvinia sp.) were grown in artificial medium (Hoagland) supplemented with 0, 1, 2, 4 and 8 ppm chromium. The plants resistance and absorption toward chromium was observed based on the morphology and chromium content in their biomass. Based on their resistance to and absorption of chromium, the selected plants were tested further in liquid wasteÃ‚Â of tanning industry. In Hoagland medium, Salvinia sp. demonstrated 67.2% higher resistance and absorption toward chromium while that of P. stratiotes 20.3% lower compared to other plants which were tested. This result could be applicable in reducing such environmental pollutant as the heavy metal chromium from industrial waste. Keywords: Phytoremediation, chromium, Hoagland medium, aquatic plants, liquid wasteÃ‚Â ABSTRAKLogam berat dalam limbah industri merupakan bahan pencemar lingkungan yang mendapatkan perhatian global. Salah satu upaya untuk mengatasi masalah tersebut adalah memanfaatkan tanaman untuk menyerap logam dalam medium cair atau dikenal sebagai fitoremediasi. Penelitian ini bertujuan untuk mengetahui tanaman airÃ‚Â lokal yang tahan dan peka secara relatif terhadap kromium. Empat spesies tanaman air (Pistia stratiotes, Eichhornia crassipes, Lemna minor, dan Salvinia sp.) ditumbuhkan pada medium buatan (Hoagland) yang dipasok kromium 0, 1, 2, 4, dan 8 ppm. Pengujian toleransi tanaman dan serapan terhadap kromium dilakukan berdasarkan pengamatan morfologis serta analisis kadar kromium dalam biomasa. Berdasarkan daya tahan dan serapan kromium, tanaman terseleksi diujikan lebih lanjut dalam limbah cairÃ‚Â industri penyamakan kulit. Dalam medium Hoagland, Salvinia sp. mempunyai ketahanan dan serapan kromium lebih tinggi sebesar 67,2% sedangkan P. stratiotes mempunyai ketahanan dan serapan kromium lebih rendah sebesar 20,3% dibandingkan tanaman lain yang diujikan. Hasil penelitian ini dapat diterapkan untuk mengurangi bahan pencemar lingkungan berupa logam berat kromium dari limbah industri.Kata Kunci: Fitoremediasi, kromium, medium Hoagland, tanaman air, limbah cair

Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste Amekan, Yumechris; Wangi, Dyah Sekar A P; Cahyanto, Muhammad Nur; Sarto, Sarto; Widada, Jaka
International Journal of Renewable Energy Development Vol 7, No 2 (2018): July 2018
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.7.2.101-109

The natural microbial consortium from many sources widely used for hydrogen production. Type of substrate and operating conditions applied on the biodigesters of the natural consortium used as inoculum impact the variation of species and number of microbes that induce biogas formation, so this study examined the effect of different inoculum source and its combination of biohydrogen production performance. The hydrogen producing bacteria from fruit waste digester (FW), cow dung digester (CD), and tofu waste digester (TW) enriched under strictly anaerobic conditions at 37OC. Inoculums from 3 different digesters (FW, CD, and TW) and its combination (FW-CD, CD-TW, FW-TW, and FW-CD-TW) were used to test the hydrogen production from melon waste with volatile solids (VS) concentration of 9.65 g/L, 37Â°C and initial pH 7.05 Â± 0.05. The results showed that individual and combined inoculum produced the gas comprising hydrogen and carbon dioxide without any detectable methane. The highest cumulative hydrogen production of 743 mL (yield 207.56 mL/gVS) and 1,132 mL (yield 231.02 mL/gVS) was shown by FW and FW-CD-TW, respectively. Butyric, acetate, formic and propionic were the primary soluble metabolites produced by all the cultures, and the result proves that higher production of propionic acid can decrease hydrogen yield. Clostridium perfringens and Clostridium baratii prominently seen in all single and combination inoculum. Experimental evidence suggests that the inoculum from different biodigesters able to adapt well to the environmental conditions and the new substrate after a combination process as a result of metabolic flexibility derived from the microbial diversity in the community to produce hydrogen. Therefore, inoculum combination could be used as a strategy to improve systems for on-farm energy recovery from animal and plant waste to processing of food and municipal waste.Article History: Received February 5th 2018; Received in revised form May 7th 2018; Accepted June 2nd 2018; Available onlineHow to Cite This Article: Amekan, Y., Wangi, D.S.A.P., Cahyanto, M.N., Sarto and Widada, J. (2018) Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste. Int. Journal of Renewable Energy Development, 7(2), 101-109.https://doi.org/10.14710/ijred.7.2.101-10

The Effects of Types of Manure and Mycorrhizal Applications on Sandy Soils on the Growth and Yield of Curly Red Chili (Capsicum annum L.) Putra, Sukmana Siswandana; Putra, Eka Tarwaca Susila; Widada, Jaka
Caraka Tani: Journal of Sustainable Agriculture Vol 35, No 2 (2020): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v35i2.34971

Market demand for chilies continues to increase, so efforts are needed to increase the productivity of the chili plants. One of the efforts is by intensifying production factors such as applying fertilizers on beach sand which contain low nutrients and organic matter so that manure input is needed and also utilizes soil microbial technology in the form of arbuscular mycorrhizal fungi which are relatively cheap economically and environmentally friendly. The purpose of this research was to study the effect of types of manure and mycorrhizal applications on the growth and yield of curly red chili. The research was arranged in a strip-plot Randomized Completely Block Design (RCBD) consisting of two factors with three replications. The first factor is the type of fertilizer (cow manure, chicken manure, guano fertilizer, quail manure and urea fertilizer) and the second factor is the application of mycorrhizae (with and without). This study concludes that quail manure increases plant height. The addition of chicken manure, guano fertilizer and quail manure multiplies the total fruit production. The application of mycorrhizae gives the same results on the growth and yield of chili.

Soil Bacterial Diversity and Productivity of Coffee - Shade Tree Agro-ecosystems Evizal, Rusdi; Tohari, .; Prijambada, Irfan Dwidja; Widada, Jaka; Widianto, Donny
JOURNAL OF TROPICAL SOILS Vol 17, No 2: May 2012
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2012.v17i2.181-187

Coffee productions should have environmental value such as providing high soil microbial diversity while producing high yield. To examine that purposes, two experimental plots were constucted at benchmark site of Conservation and Sustainable Management of Below-Ground Biodiversity (CSM-BGBD), in Sumberjaya Subdistrict, West Lampung, Indonesia, during 2007-2010. Types of coffee agro-ecosystem to be examined were Coffea canephora with shade trees of Gliricidia sepium, Erythrina sububrams, Michelia champaca, and no shade. Two plots were constructed at 5-years-coffee and 15-years-coffee. Diversity of soil bacteria was determined based on DNA finger printing of total soil bacteria using Ribosomal Intergenic Spacer Analysis (RISA) method. The results showed that: (1) For mature coffee (15 years old), shade-grown coffee agro-ecosystems had higher soil bacterial diversity than those of no shade coffee agro-ecosystem, (2) Shaded coffee agro-ecosystems were able to conserve soil bacterial diversity better than no-shade coffee agro-ecosystem. Soil organic C and total litter biomass had positive effect on soil bacterial diversity, (3) Types of agro-ecosystem significantly affect the bean yield of 15 years coffee. Coffee agro-ecosystems shaded by legume trees had higher yield than those of non-legume shade and no shade coffee agro-ecosystem, (4) Shannon-Weaver indices of soil bacterial diversity together with weed biomass and N content of coffee leaf had positive effect on coffee bean yield.

The Role of Indigenous Mycorrhizae of Corn Plants in Various Soil Types in Gunung Kidul, Indonesia Diannastiti, Fani Aulia; Utami, Sri Nuryani Hidayah; Widada, Jaka
PLANTA TROPIKA Vol 10, No 1 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i1.11428

Indigenous Vesicular-Arbuscular Mycorrhizae (VAM) are natural mycorrhizae from specific areas that have good environmental adaptability. This study, conducted from January to November 2020 at the Faculty of Agriculture, Universitas Gadjah Mada, aimed to isolate the vesicular-arbuscular mycorrhizal fungus so that it can be used as information on the type and role of VAM on Gunung Kidul soil. The research was arranged in a Completely Randomized Design (CRD) with three factors. The first factor was soil type from Gunung Kidul Regency (Inceptisol, Mollisol, and Alfisol), the second factor was sterilization (sterilized soil and unsterilized soil), and the third factor was corn variety (local and hybrid). Analysis of soil and plant growth was performed by using Analysis of Variance (ANOVA) and Tukey's Honestly Significant Difference (Tukey's HSD) Test. Genetic detection of root infecting VAM was performed by using Terminal Restriction Fragment Length Polymorphism (T-RFLP) method with FAM AML1-AML2 labeled primers. The VAM detected in the roots of hybrid variety included Acaulospora sp., Gigaspora sp., and Septoglomus sp., and those in the roots of local variety were Acaulospora sp., Gigaspora sp., and Funelisformis sp. The results showed that the role of VAM could be seen through unsterilized soil so that there was no VAM elimination in the soil. Unsterilized soil showed the best results of root infection, leaf fresh and dry weight, leaf phosphor (P) content, and leaf P uptake. Meanwhile, Alfisol showed the best result of root infection, fresh weight, dry weight, leaf P content, and leaf P uptake. The treatment of plant varieties showed that the varieties did not significantly affect the root infection, fresh weight, dry weight, leaf P content, and leaf P uptake.

Distribution of Ammonium-Oxidizing Bacteria in Sediment with Relation to Water Quality at the Musi River, Indonesia Melki Melki; Alim Isnansetyo; Jaka Widada; Murwantoko Murwantoko
HAYATI Journal of Biosciences Vol. 25 No. 4 (2018): October 2018
Publisher : Bogor Agricultural University, Indonesia

The Musi River is located in the southern Sumatra, Indonesia. Most of activities, i.e. agricultural, industrial, and urban activities are considered as being major sources of chemicals and nutrients with their waste products effluent into the river. Nitrification, the microbial oxidation of ammonia to nitrite and nitrate, occurs in a wide variety of environments and naturally remove anthropogenic N pollution. The purpose of this research was to determine of distribution of ammonium-oxidizing bacteria (AOB) in sediment with relation to water quality at the Musi river area. This study was conducted on rainy and dry season 2016 at five sampling sites from the freshwater to seawater at high and low tide conditions, the sampling sites are station St1 (Gandus), station St2 (Palembang city), station St3 (Upang), station St4 (Sungsang), and station St5 (Sea). Sediment samples were collected from the surface layer by using an Ekman grab. Some water quality such as salinity, temperature, pH, and dissolved oxygen (DO) were directly analyzed in the field, while other water quality such as NH4-N, NO2-N, and NO3-N were analyzed in the laboratory. The Density of AOB was determined by the most probable number of (MPN) method. The PCA was used to correlate variations of the AOB with physicochemical properties using software Xlstat. The results showed that the physicochemical properties had a range of salinity of 0 to 20 ppt, temperature of 29.21 to 31.82oC, pH of 4.88 to 7.93, DO of 3.44 to 11.33 mg/l, NH4-N in sediment of 0.04 to 0.87 mg/l, NO2-N in sediment of 0.01 to 1.77 mg/l, NO3-N in sediment of 0.09 to 2.08 mg/l. The density of AOB ranged from 7.2 x 102 to 6.1 x 103 cells/g sediment. Principal component analyses showed that temperature, pH, DO, and concentrations of nutrient contributed to density of AOB.

FITOREMEDIASI KANDUNGAN KROMIUM PADA LIMBAH CAIR MENGGUNAKAN TANAMAN AIR Anna Safarrida; Ngadiman .; Jaka Widada
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Existence of heavy metals in industrial waste is gaining global attention since their negative impact to environment. One of the efforts to solve the problem was to use plant to absorb metal in liquid medium, known as rhizofiltration. This research was aimed to select aquatic plantsÂ which showed relative resistantce and susceptibility to chromium. Four species of aquatic plants (Pistia stratiotes, Eichhornia crassipes, Lemna minor and Salvinia sp.) were grown in artificial medium (Hoagland) supplemented with 0, 1, 2, 4 and 8 ppm chromium. The plants resistance and absorption toward chromium was observed based on the morphology and chromium content in their biomass. Based on their resistance to and absorption of chromium, the selected plants were tested further in liquid wasteÂ of tanning industry. In Hoagland medium, Salvinia sp. demonstrated 67.2% higher resistance and absorption toward chromium while that of P. stratiotes 20.3% lower compared to other plants which were tested. This result could be applicable in reducing such environmental pollutant as the heavy metal chromium from industrial waste. Keywords: Phytoremediation, chromium, Hoagland medium, aquatic plants, liquid wasteÂ ABSTRAKLogam berat dalam limbah industri merupakan bahan pencemar lingkungan yang mendapatkan perhatian global. Salah satu upaya untuk mengatasi masalah tersebut adalah memanfaatkan tanaman untuk menyerap logam dalam medium cair atau dikenal sebagai fitoremediasi. Penelitian ini bertujuan untuk mengetahui tanaman airÂ lokal yang tahan dan peka secara relatif terhadap kromium. Empat spesies tanaman air (Pistia stratiotes, Eichhornia crassipes, Lemna minor, dan Salvinia sp.) ditumbuhkan pada medium buatan (Hoagland) yang dipasok kromium 0, 1, 2, 4, dan 8 ppm. Pengujian toleransi tanaman dan serapan terhadap kromium dilakukan berdasarkan pengamatan morfologis serta analisis kadar kromium dalam biomasa. Berdasarkan daya tahan dan serapan kromium, tanaman terseleksi diujikan lebih lanjut dalam limbah cairÂ industri penyamakan kulit. Dalam medium Hoagland, Salvinia sp. mempunyai ketahanan dan serapan kromium lebih tinggi sebesar 67,2% sedangkan P. stratiotes mempunyai ketahanan dan serapan kromium lebih rendah sebesar 20,3% dibandingkan tanaman lain yang diujikan. Hasil penelitian ini dapat diterapkan untuk mengurangi bahan pencemar lingkungan berupa logam berat kromium dari limbah industri.Kata Kunci: Fitoremediasi, kromium, medium Hoagland, tanaman air, limbah cair

PENGARUH INOKULASI JAMUR MIKORIZA ARBUSKULA TERHADAP GLOMALIN, PERTUMBUHAN DAN HASIL PADI Jauhari Syamsiyah; Bambang Hendro Sunarminto; Eko Hanudin; Jaka Widada
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 11, No 1 (2014)
Publisher : Faculty of Agriculture, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v11i1.214

TITLE : EFFECT OF ARBUSCULAR MYCORRIZHAL FUNGI INOCULATION ON GLOMALIN, GROWTH AND RICE YIELD. The aim of this study was to measure the glomalin production, growth and yield of rice in upland rice fields. The research used Completely Randomized Design with four treatments: two sterilization levels (no sterilisatio and with sterilization) and two levels of mycorrhizae inoculation ( no mycorrhizae and with mycorrhizae). Mycorrhizae (5 g/pot) was inoculated before planting rice seeds. The results showed that Glomalin Total (GT) and Glomalin easily extracted (GEE) were higher in mycorrhizae inoculation, increased by 16% and 20% in non-sterile soil and 25% and 11% in sterile soil compared to non-mycorrhizae inoculation. The content of the GT ranged from 4.95 to 9.74 mg/g soil and GEE 0.99 to 2.78 mg/g soil. Plant height, tillers number and the grain yield was increased by mycorrhizae inoculation significantly. Soil sterilization could reduce the effectiveness of mycorrhizae inoculated.

Co-Authors , Tamad . Tohari A. Alimuddin Achmad Dinoto Adi Laksono Ahmad Romdhon Akira Hosoyama Akira Hosoyama Ali Ikhwan Alim Isnansetyo Amekan, Yumechris Angga Prasetya Anna Safarrida Anna Safarrida, Anna Annisa Yusuf, Wahida Ariani Hatmanti Arif Muliawan Arifah Khusnuryani Arifah Khusnuryani Ariyanti, Nur Fitriana Asrul Asrul Asrul Asrul Atsushi Yamazoe Atsushi Yamazoe Aziz Purwantoro Azwar Maas Bambang Hadisutrisno Bambang Hadisutrisno Bambang Hadisutrisno Bambang HADISUTRISNO Bambang Hariwiyanto Bambang Hariwiyanto Bambang Hendro Sunarminto Bambang Hendro sunarminto Bostang Radjagukguk Camelia Herdini Christanti Sumardiyono Denny Irawati Dewi Seswita Zilda DEWI SESWITA ZILDA Dhani Suryawan Dhani Suryawan, Dhani Diani Mentari Diannastiti, Fani Aulia Didik Indradewa Didik Indradewa Didik Indradewa Dinar Mindrati Fardhani Dionysius Andang Arif Wibawa Dionysius Andang Arif Wibawa, Dionysius Andang Dody Kastono Dolly Iriani Damarjaya Dolly Iriani Damarjaya, Dolly Iriani Donny Widianto Donny Widianto Donny Widianto Donny Widianto Dyah Weny Respatie Edy Meiyanto Eka Tarwaca Susila Putra Eko Hanudin Eko Hanudin EKO IRIANTO Ema Damayanti Ema Damayanti Endang Semiarti Endang Sutriswati Rahayu Eni Harmayani ENI HARMAYANI Erni Martani Erni Martani Eti Nurwening Sholikhah Fatturahman Ridwan, Nur Febriansah , Rifki Galang Indra Jaya Ganis Lukmandaru Gintung Patantis Gintung Patantis GINTUNG PATANTIS Hadi, Ismanurrahman Hari Eko Irianto Hartono Hartono H, Hartono Hera Nirwati Hideaki Nojiri Hideaki Nojiri Indun Dewi Puspita IRFAN D. PRIJAMBADA Irfan Dwidya Prijambada Irwan Suluk Padang Jauhari Syamsiyah Joko Sulistyo Kana Ninomiya Keishi Senoo Keishi Senoo, Keishi Khoirun Nisa Lucia Dhiantika Witasari Lucia Dhiantika Witasari, Lucia Dhiantika M. Mustofa Maria Gratias Mariyatun Mariyatun, Mariyatun Masagus Muhammad Prima Putra Masaya Nishiyama Masaya Nishiyama, Masaya Ma’as, Azwar Melki Melki Mirtani Naima Mohamad Aji Ikhrami Muhammad Dylan Lawrie Muhammad Nur Cahyanto Muhammad Saifur Rohman Mujiyo Mujiyo Mukhlissul Faatih Mukhlissul Faatih1, Mukhlissul Mulyadi Mulyadi Mulyadi Mulyadi Mulyono Mulyono Murwantoko . Mustofa M, Mustofa Mustofa Mustofa Mustofa Mustofa N. Ngadiman Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ngadiman Ngadiman . Ngadiman ., Ngadiman Ngadiman N, Ngadiman Noviyanto, Amir Nunuk Supriyatno Nur Edy Nur Prihatiningsih Nurfiani, Sri Ocky Karna Radjasa Oedjijono Oedjijono, Oedjijono Pintaka Kusumaningtyas Prijambada, Irfan Dwidja PUSPITA LISDIYANTI Putra, Sukmana Siswandana Putu Sudira R. Riyanti Rahayu, Endang Sutriswati Reni Nurjasmi Reni Nurjasmi, Reni Riska Wulansari Ristiarini, Susana Riyanti Riyanti Rusdi Evizal Saipul Abbas Sarto SATRIYAS ILYAS Shigeto Otsuka Shigeto Otsuka, Shigeto Shinta Hartanto Shogo Matsumoto Sigit Sunarta, Sigit SITI KABIRUN Siti Kabirun Siti Kabirun Siti Subandiyah Sofia Mubarika Haryana Sri Hastuti Sri Nopitasari Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami Sri Suryanti Sri Suryanti Sri Wedhastri Stalis Norma Ethica Subagus Wahyuono Sudadi Sudadi Suhartiningsih Dwi Nurcahyanti Sumarno Sumarno Sumarno Sumarno Suryanti Suryanti Suryanti Suryanti Susila Herlambang TOHARI TOHARI Tohari Tohari Tomy Listyanto Tomy Listyanto Tri Harjaka Tri Joko Raharjo Tri Rini Nuringtyas Tri Wibawa Tri Wibawa Triwibowo Yuwono Triwidodo Arwiyanto Wangi, Dyah Sekar A P Widya Asmara WIDYA ASMARA Wulansari, Riska Yani Lestari Nuraini Yani Lestari Nuraini, Yani Lestari Yasushi Yoshioka Yenny Sariasih Yose Rizal Yuli Setiawati Yuliana Prahastiwi Yuliana Yuliana Prahastiwi, Yuliana Yusro Nuri Fawzya Yusro Nuri Fawzya YUSRO NURI FAWZYA Yuuki Asano Yuyun Farida Yuyun Farida, Yuyun Zilda, Dewi Zeswita

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