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All Journal Jurnal Sain Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter JURNAL PETERNAKAN Jurnal Peternakan Indonesia Vitek : Bidang Kedokteran Hewan J-Kesmas: Jurnal Kesehatan Masyarakat (Indonesian Journal of Public Health) Arwana: Jurnal Ilmiah Program Studi Perairan The International Journal of Tropical Veterinary and Biomedical Research Buletin Pengabdian Jurnal Medika Veterinaria
Masda Admi
Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Isolation and Identification of Gram-Negative Bacteria on Cattle Farms Indicated by Mastitis Ayuti, Siti Rani; Pratiwi, Zahwa Amelia; Admi, Masda; Darniati, Darniati; Isa, M; Herrialfian, Herrialfian
The International Journal of Tropical Veterinary and Biomedical Research Vol 7, No 2 (2022): Vol. 7 (2) November 2022
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v7i2.30735

Abstract

Mastitis is oneofthe causesofdiseases that can reduce livestock production.Mastitis can be caused by various types of bacteria that cause a decrease in livestock production that is difficult to cure with antibiotics has been reported resistance. This study aims to determine gram-negative bacteria that can be the cause of mastitis in cows. The study used samples taken from folk farms by means of livestock udder swabs. Bacterial isolation is carried outbyplanting in Mannitol Salt Agar (MSA) mediaandidentifying by Gram stainingandbiochemical tests (maltose and lactose). The data analysis used is descriptive analysis, namely the types of bacteria as the causative agents of mastitis.The results of the study obtained bacteria that cause mastitis ofgram-negative speciesKlebsiellaspandEnterobactersp yang classified as Gram-negativebacteria. It can be concluded that in cows indicated mastitis there are Gram-negative bacteria, these are morphologically identified, namelyKlebsiellaspandEnterobactersp.
Isolasi, Identifikasi dan Uji Sensitivitas Bakteri Streptococcus SP. Penyebab Endometritis Subklinis pada Sapi Aceh Admi, Masda; Kurniawan, Wilda; Dasrul, Dasrul
Jurnal Peternakan Vol 21, No 2 (2024): September 2024
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v21i2.24172

Abstract

ABSTRAK. Penelitian ini bertujuan untuk isolasi, identifikasi dan uji sensitivitas antibiotik eritromisin dan penisilin G terhadap bakteri Streptococcus sp. penyebab endometritis subklinis pada sapi aceh. Penelitian ini dilakukan dengan cara swab dibagian vagina sapi  sebagai sampel, dan dimasukkan ke dalam tabung reaksi berisi Nutrien Broth (NB), kemudian diisolasi menggunakan media Manitol Salt Agar (MSA) dan diidentifikasi koloni bakteri menggunakan pewarnaan Gram, serta dilakukan uji katalase. Hasil isolasi bakteri tersebut diuji sensitivitas antibiotik dengan cara menempelkan disk antibiotik eritromisin dan disk antibiotik penisilin G pada permukaan media Mueller Hinton Agar (MHA) dan setelah inkubasi pada suhu 37 ­­­0C selama 24 jam diamati zona bening yang terbentuk disekitar disk antibiotik tersebut. Hasil penelitian menunjukkan adanya pertumbuhan bakteri pada media NB dan terdapat koloni terpisah pada media MSA yang menunjukkan Gram positif berbentuk kokus dan tidak menimbulkan gas pada uji katalase sebagai reaksi spesifik dari bakteri Streptococcus sp. Hasil isolasi bakteri dari vagina sapi aceh tersebut, secara uji sensitivitas menunjukkan adanya diameter zona bening seluas 23,86 mm pada antibiotik eritromisin dan 8,86 mm pada antibiotik penisilin G. Hasil penelitian dapat disimpulkan bahwa pada vagina sapi aceh ditemukan adanya bakteri Streptococcus sp. yang berpotensi menyebabkan endometritis subklinis dan sensitif terhadap antibiotik eritromisin serta resisten terhadap penisilin G.Kata kunci: antibiotik, endometritis subklinis, sapi aceh, Streptococcus sp.Isolation, Identification and Sensitivity Test to Antibiotics of Bacteria Streptococcus sp. Causes of Subclinical Endometritis in Aceh CattleABSTRACT. This study aims to isolate, identify, and test the sensitivity of erythromycin and penicillin G antibiotics against Streptococcus sp. bacteria causing subclinical endometritis in Aceh cattle. The study was conducted by swabbing the vaginal area of the cattle to collect samples, which were then placed in test tubes containing Nutrient Broth (NB). The bacteria were isolated using Manitol Salt Agar (MSA) media and identified by Gram staining, along with a catalase test. The isolated bacteria were tested for antibiotic sensitivity by applying erythromycin and penicillin G antibiotic disks onto the surface of Mueller Hinton Agar (MHA) media. After incubation at 37°C for 24 hours, the clear zones formed around the antibiotic disks were observed. The results showed bacterial growth on NB media and separate colonies on MSA media, which were Gram-positive cocci bacteria and did not produce gas in the catalase test, indicating a specific reaction of Streptococcus sp. The sensitivity test results showed a clear zone diameter of 23.86 mm for erythromycin and 8.86 mm for penicillin G. This study concluded that Streptococcus sp. bacteria found in the vagina of Aceh cattle have the potential to cause subclinical endometritis and are sensitive to erythromycin but resistant to penicillin G.
Isolation and identification of Pseudomonas sp. on dumbo catfish (Clarias gariepinus) in semi-intensive manner Admi, Masda; Maulida, Rafdhayatul; Helmi, Teuku Zahrial; Winaruddin, Winaruddin; Akmal, Yusrizal
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 6 No 2: November 2024
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v6i2.2956

Abstract

The aquaculture system in the maintenance of dumbo catfish is widely practiced by the community due to several advantages it offers. However, in terms of fish health, there is a risk of infection by Pseudomonas bacteria present in the environment. This study aims to isolate and identify Pseudomonas sp. bacteria in dumbo catfish (Clarias gariepinus) raised in a semi-intensive manner. A total of three samples of dumbo catfish were taken from ponds in Beurangong Village, Kuta Baro District, and nine samples from ponds in Dham Pulo Village, Ingin Jaya District, Aceh Besar Regency. The criteria for the samples were fish that clinically showed symptoms of disease caused by Pseudomonas sp. Each catfish had samples taken from the skin, liver, spleen, intestines, and gills for subsequent bacterial isolation and identification using the Carter method. Bacterial isolation was conducted using Nutrient Broth (NB) media. Identification utilized Plate Count Agar (PCA) media, Gram staining, and biochemical tests. The results showed bacterial growth in the NB media, and Pseudomonas bacteria were successfully isolated. The identification results on PCA media indicated Pseudomonas sp., and the biochemical tests identified it as Pseudomonas aeruginosa. This study concludes that in dumbo catfish raised semi-intensively, Pseudomonas sp. bacteria were isolated and identified in Beurangong Village, while in Dham Pulo Village, the identified species was Pseudomonas aeruginosa.
Aktivitas Ekstrak Etanol Daun Bandotan Terhadap Pertumbuhan Bakteri Vibrio sp. Isolat Ikan Lele Dumbo (Clarias gariepinus) Admi, Masda; Fitri, Khairani; Hennivanda, Hennivanda; Jamin, Faisal; Rosmaidar, Rosmaidar; Syafruddin, Syafruddin; Winaruddin, Winaruddin; Azhari, Azhari
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.96568

Abstract

     Daun bandotan merupakan salah satu tanaman obat yang banyak dimanfaatkan  dalam mengobati berbagai jenis penyakit karena mengandung senyawa fitokimia yang dapat menghambat pertumbuhan bakteri seperti alkaloid, flavonoid, tanin, saponin dan steroid. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol daun bandotan terhadap pertumbuhan bakteri Vibrio sp. isolat ikan lele dumbo. Pelaksanaan penelitian ini menggunakan metode Kirby Bauer disk diffusion yang diawali dengan peremajaan stok isolat bakteri pada media Nutrient Broth, reidentifikasi dengan media Thiosulfate Citrate Bile Salt Sucrose Agar dan pewarnaan Gram. Selanjutnya dilakukan uji daya hambat menggunakan media Mueller Hinton Agar. Studi ini terdiri dari 5 perlakuan yaitu ekstrak etanol daun bandotan konsentrasi 25% (P1), 50% (P2), dan 75% (P3), siprofloksasin sebagai kontrol positif dan CMC 1% sebagai kontrol negatif. Hasil penelitian menunjukkan diameter zona hambat yang terbentuk pada kontrol positif (45,13 ± 0,04mm) dua kali lebih besar dibandingkan daya hambat yang terbentuk pada ekstrak daun bandotan konsentrasi 25% (23,30 ± 0,15mm), konsentrasi 50% (24,29 ± 0,17 mm), dan konsentrasi 75% (25,29 ± 0,16mm), kontrol negatif tidak menunjukkan terbentuknya zona hambat. Kesimpulan penelitian adalah ekstrak etanol daun bandotan berpotensi dapat menghambat pertumbuhan bakteri Vibrio sp. hasil isolat lele dumbo.      
The EFEKTIVITAS EKSTRAK ETANOL DAUN BANDOTAN (Ageratum conyzoides) TERHADAP Salmonella enterica serovar typhimurium SECARA IN VITRO: : daniel, daniel daniel; admi, masda; putri, anggun bellia; helmi, teuku zahrial; wardhani, lailia dwi Kusuma
VITEK : Bidang Kedokteran Hewan Vol. 14 No. 1 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i1.281

Abstract

Bandotan leaves (Ageratum conyzoides) are one of the traditional medicinal plants that are trusted empirically by the community to treat various diseases. Several studies report the potential of bandotan leaves as an antibacterial because they contain the chemical compounds trepanoids, flavonoids, alkaloids, essential oils, and tannins. This study aims to determine the effectiveness of bandotan leaves on the growth of Salmonella enterica serovar typhimurium bacteria in vitro. This research used the Kirby Bauer disk diffusion method with the antibiotic chloramphenicol as a positive control, CMC 1% as a negative control, and an ethanol extract of bandotan leaves with concentrations of 25%, 50%, and 75%. Re-identification of bacteria was carried out using Gram staining, and effectiveness testing was carried out on Mueller Hinton Agar (MHA) media. The results of the study on Gram staining showed uniform morphological characteristics of Salmonella enterica serovar Typhimurium bacteria and the diameter of the inhibition zone formed at concentrations of bandotan leaf ethanol extract of 25% (8.02 mm ± 0.41), 50% (8.37mm ± 0.69) and 75% (8.78mm ± 0.78). It can be concluded that the ethanol extract of bandotan leaves at concentrations of 25%, 50%, and 75% has a weak category of inhibitory activity against the growth of Salmonella enterica serovar Typhimurium bacteria in vitro.
Sensitivity Test Of Ethanol Extract Of Senggani Leaves (Melastoma Candidum) On The Growth Shigella Atcc 25931 In Vitro Rosmaidar, Rosmaidar; Admi, Masda; Riady, Ginta; Isa, M.; Hamzah, Abdullah; Sabri, Mustafa; Nurzalika, Siti
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.35157

Abstract

Senggani leaves are herbal plants that have antibacterial properties and contain phytochemical compounds in the form of flavonoids, saponins, tannins, steroids, and glycosides. The purpose of this study was to determine the sensitivity of Senggani (Melastoma candidum) leaf extract to the inhibition of the growth of Shigella ATCC 25931 in vitro. Reidentification of bacteria was carried out by Gram staining, and the sensitivity test was conducted on Mueller Hinton Agar media (MHA). Chloramphenicol was used as a positive control, and 1% CMC was used as a negative control. The results of the Gram staining showed Shigella ATCC 25931 bacteria, and the inhibition zone formed at each concentration was 25% (8.26 0.096 mm), 50% (9.02 0.158 mm), and 75% (9.83 0.100 mm). It can be concluded that all concentrations of the ethanol extract of senggani leaves have inhibitory sensitivity to the growth of Shigella ATCC 25931 in the weak category because the inhibition zone formed was 12 mm."
The Isolation of Staphylococcus Epidermidis Bacteria in White Snapper Salted Fish (Lates Calcalifer) of Sibolga City, North Sumatera Province Lestari, Rafika; Admi, Masda; Rastina, Rastina; Dewi, Maryulia; Nurliana, Nurliana; Harris, Abdul; Riady, Ginta
J-Kesmas: Jurnal Fakultas Kesehatan Masyarakat (The Indonesian Journal of Public Health) Vol 7, No 1 (2020): April 2020
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/j-kesmas.v7i1.1918

Abstract

Salted fish is vulnerable to contamination by microbial. Staphylococcus epidermidis is one of the bacteria that can contaminate the salted fish. This research aims to isolate the Staphylococcus epidermidis bacteria contamination in salted white snapper fish sold in Sibolga City, North Sumatra Province. The sample used was white snapper salted fish, amounting to 10 samples from 10 traders. The isolation of Staphylococcus epidermidis was carried out using the Carter method. White snapper salted fish are mashed using a blender, then planted on Nutrient Broth (NB) as a bacterial growth media. Furthermore, identification of bacterial colonies grew using gram staining, Manitol Salt Agar (MSA) media, Blood Agar Plate (BAP) media, catalase test and confectionery media (Manitol and Glucose). The data obtained were analyzed descriptively. The results of this study suggest that Staphylococcus epidermidis contains bacterial contamination. Based on the data collected, it can be concluded that 7 out of 10 samples of white snapper salted fish sold in Sibolga City, North Sumatra Province, are contaminated with 70 percent Staphylococcus epidermidis bacteria.
Herbal flavonoids in preserving testicular physiology under oxidative stress: A bibliometric analysis of systematic reviews Zulkifli, Baidillah; Fakri, Fajar; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Sabri, Mustafa; Lubis, Triva Murtina; Zainuddin, Zainuddin; Zulfitri, Zulfitri; Admi, Masda; Syah, Nanda Yulian; Usrina, Nora; Fajri, Fauzan
The International Journal of Tropical Veterinary and Biomedical Research Vol 8, No 2 (2023): Vol. 8 (2) November 2023
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v8i2.36616

Abstract

Herbal antioxidants have gained attention recently as possible treatments for disorders of male reproductive function linked to oxidative stress because of their ability to neutralize free radicals.The investigation into herbal flavonoids for preserving oxidative stress-impaired testicular physiology has been effectively conducted through the visualization of bibliometric analysis. Bibliometric data of published papers collected from the PubMed and Scopus databases in 2018-2023. Network visualization was performed using VOSviewer software on the set keywords that occur (n=15). The literature review was conducted according to the research trends identified by the clusters that appeared in the network visualization. The result revealed that a total of 266 records have been published based on the specified search set keywords since 2018. Through the analysis of keywords, inclusion and exclusion criteria, and the relevant content of papers, 11 articles were identified as suitable for conducting a literature review. Network visualization revealed that the research falls into two general categories: (1) toxicity to the testicles caused by oxidative stress and (2) prevention of oxidative stress impact on tissue through herbal medicine. Examination of the paper's content revealed that certain antioxidant compounds from plants can stabilize free radicals in the testicles. These antioxidants include vitexin, diosmin, hesperidin, phenolic acids, naphthoquinones, fisetin, pachypodol, sciadopitysin, carvacrol, and morin. However, further research is needed to compare active antioxidant content in different herbal plants and identify optimal concentrations for therapeutic effects.
Co-Authors - Fakhrurrazi . Darniati . Nurliana Abd. Rasyid Syamsuri Abdul Harris Abdullah Hamzah, Abdullah Ali Makmur Amalia Sutriana Amiruddin Amiruddin Annisa Anwar Sitorus Ayuti, Siti Rani Azhari Azhari Azhari Azhari Azhari, A Daniel, Daniel Daniel Darmawi Darmawi Darmawi Darmawi Darniati Darniati Dasrul Dasrul Erina Erina Erina Erina Erina Erina Faisal Jamin Faisal Mustafa Fajar Fakri Fajri, Fauzan Fakhrurrazi Fakhrurrazi Fitri, Khairani Gholib Gholib, Gholib Ginta Riady Helmi, T. Zahrial Hendri Syah Hennivanda Hennivanda Hennivanda, Hennivanda Herrialfian Herrialfian Herrialfian, Herrialfian Isa, M Kurniawan, Wilda Lestari, Rafika M Daud AK M Isa M Isa M.iSA - Mahdi Abrar Maryulia Dewi Maryulia Dewi Maulida, Rafdhayatul Muhammad Hambal Muhammad Walidin Mulyadi Adam, Mulyadi Muslim Akmal Nanda Yulian Syah Nora Usrina NURLIANA NURLIANA Nurzalika, Siti Pratiwi, Zahwa Amelia putri, anggun bellia Rasmaidar Rasmaidar Rastina, Rastina Rinidar Rinidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Siti Rani Ayuti Siti Rani Ayuti Sugito Sugito Syafruddin Syafruddin T Zahrial Helmi T Zahrial Helmi T. Fadrial Karmil T. Fadrial Karmil Teuku Zahrial Helmi Triva Murtina Lubis Ummu Balqis Wardhani, Lailia Dwi Kusuma Widya Nur Hidayati Winaruddin Winaruddin Yuni Sari Yusrizal Akmal Zahwa Amelia Pratiwi Zainuddin Zainuddin Zainuddin, Zainuddin Zulfitri Zulfitri, Zulfitri Zulkifli, Baidillah