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Sensitivity Test Of Ethanol Extract Of Senggani Leaves (Melastoma Candidum) On The Growth Shigella Atcc 25931 In Vitro Rosmaidar, Rosmaidar; Admi, Masda; Riady, Ginta; Isa, M.; Hamzah, Abdullah; Sabri, Mustafa; Nurzalika, Siti
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.35157

Abstract

Senggani leaves are herbal plants that have antibacterial properties and contain phytochemical compounds in the form of flavonoids, saponins, tannins, steroids, and glycosides. The purpose of this study was to determine the sensitivity of Senggani (Melastoma candidum) leaf extract to the inhibition of the growth of Shigella ATCC 25931 in vitro. Reidentification of bacteria was carried out by Gram staining, and the sensitivity test was conducted on Mueller Hinton Agar media (MHA). Chloramphenicol was used as a positive control, and 1% CMC was used as a negative control. The results of the Gram staining showed Shigella ATCC 25931 bacteria, and the inhibition zone formed at each concentration was 25% (8.26 0.096 mm), 50% (9.02 0.158 mm), and 75% (9.83 0.100 mm). It can be concluded that all concentrations of the ethanol extract of senggani leaves have inhibitory sensitivity to the growth of Shigella ATCC 25931 in the weak category because the inhibition zone formed was 12 mm."
The Isolation of Staphylococcus Epidermidis Bacteria in White Snapper Salted Fish (Lates Calcalifer) of Sibolga City, North Sumatera Province Lestari, Rafika; Admi, Masda; Rastina, Rastina; Dewi, Maryulia; Nurliana, Nurliana; Harris, Abdul; Riady, Ginta
J-Kesmas: Jurnal Fakultas Kesehatan Masyarakat (The Indonesian Journal of Public Health) Vol 7, No 1 (2020): April 2020
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/j-kesmas.v7i1.1918

Abstract

Salted fish is vulnerable to contamination by microbial. Staphylococcus epidermidis is one of the bacteria that can contaminate the salted fish. This research aims to isolate the Staphylococcus epidermidis bacteria contamination in salted white snapper fish sold in Sibolga City, North Sumatra Province. The sample used was white snapper salted fish, amounting to 10 samples from 10 traders. The isolation of Staphylococcus epidermidis was carried out using the Carter method. White snapper salted fish are mashed using a blender, then planted on Nutrient Broth (NB) as a bacterial growth media. Furthermore, identification of bacterial colonies grew using gram staining, Manitol Salt Agar (MSA) media, Blood Agar Plate (BAP) media, catalase test and confectionery media (Manitol and Glucose). The data obtained were analyzed descriptively. The results of this study suggest that Staphylococcus epidermidis contains bacterial contamination. Based on the data collected, it can be concluded that 7 out of 10 samples of white snapper salted fish sold in Sibolga City, North Sumatra Province, are contaminated with 70 percent Staphylococcus epidermidis bacteria.
Herbal flavonoids in preserving testicular physiology under oxidative stress: A bibliometric analysis of systematic reviews Zulkifli, Baidillah; Fakri, Fajar; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Sabri, Mustafa; Lubis, Triva Murtina; Zainuddin, Zainuddin; Zulfitri, Zulfitri; Admi, Masda; Syah, Nanda Yulian; Usrina, Nora; Fajri, Fauzan
The International Journal of Tropical Veterinary and Biomedical Research Vol 8, No 2 (2023): Vol. 8 (2) November 2023
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v8i2.36616

Abstract

Herbal antioxidants have gained attention recently as possible treatments for disorders of male reproductive function linked to oxidative stress because of their ability to neutralize free radicals.The investigation into herbal flavonoids for preserving oxidative stress-impaired testicular physiology has been effectively conducted through the visualization of bibliometric analysis. Bibliometric data of published papers collected from the PubMed and Scopus databases in 2018-2023. Network visualization was performed using VOSviewer software on the set keywords that occur (n=15). The literature review was conducted according to the research trends identified by the clusters that appeared in the network visualization. The result revealed that a total of 266 records have been published based on the specified search set keywords since 2018. Through the analysis of keywords, inclusion and exclusion criteria, and the relevant content of papers, 11 articles were identified as suitable for conducting a literature review. Network visualization revealed that the research falls into two general categories: (1) toxicity to the testicles caused by oxidative stress and (2) prevention of oxidative stress impact on tissue through herbal medicine. Examination of the paper's content revealed that certain antioxidant compounds from plants can stabilize free radicals in the testicles. These antioxidants include vitexin, diosmin, hesperidin, phenolic acids, naphthoquinones, fisetin, pachypodol, sciadopitysin, carvacrol, and morin. However, further research is needed to compare active antioxidant content in different herbal plants and identify optimal concentrations for therapeutic effects.
A one health perspective on antimicrobial susceptibility testing and multidrug resistance patterns of Escherichia coli from exotic pets in Banda Aceh, Indonesia Ferasyi, Teuku Reza; Abrar, Mahdi Abrar; Ismail, Ismail; Admi, Masda; Sari, Wahyu Eka; Awaluddin, Awaluddin; Rosalia, Rosalia; Wardhani, Lailia Dwi Kusuma; Zulkifli, Baidillah; Rosa, Teuku Shaddiq; Kamaruzaman, Intan Noor Aina; Hemelikova, Adela
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.272-290

Abstract

Antimicrobial-resistant Escherichia coli is widely used as an indicator organism in antimicrobial resistance (AMR) surveillance, with most studies focusing on livestock. However, AMR in companion animals, particularly exotic pet species in Indonesia, remains poorly explored. This preliminary study aimed to presumptively detect resistant E. coli in pigeons, civets, and iguanas housed in pet shops located on Seutui’s main road in the city of Banda Aceh, Indonesia. A descriptive cross-sectional study was conducted to collect 15 fecal samples from pigeons (n = 9), civets (n = 3), and iguanas (n = 3) kept in three pet shops from June to July 2025. E. coli isolates were confirmed using Eosin Methylene Blue agar (EMBA). Antibiotic susceptibility testing (AST) was performed using the Kirby–Bauer method against five antibiotics: cephalothin (CEP, 30 μg), sulfamethoxazole/trimethoprim (SXT, 25 μg), ciprofloxacin (CIP, 5 μg), tetracycline (TE, 30 μg), and chloramphenicol (CHL, 30 μg). Presumptive E. coli positivity was confirmed in 46.67% (7/15) of the fecal samples. Species-specific positivity rates were 44.44% (4/9), 33.33% (1/3), and 66.67% (2/3) in pigeons, civets, and iguanas, respectively. The multidrug resistance (MDR) profile across all isolates was 85.71% (6/7). Species-specific MDR was observed in 75.00% (3/4) of pigeon-derived isolates and 100% of both civet-derived (n = 1) and iguana-derived isolates (n = 2). In conclusion, multidrug-resistant E. coli was detected in pigeons, civets, and iguanas housed in pet shops. A One Health approach and mitigation framework should be implemented to control AMR in pet shops and other relevant veterinary settings.
ISOLASI DAN IDENTIFIKASI BAKTERI Enterobacter sp. PADA BURUNG KUNTUL BESAR (Egretta alba) DI KECAMATAN BAITUSSALAM Mutia Sukma Langkana; Masda Admi; Azhari; Fakhrurrazi; Razali Daud
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 12 No. 1 (2026): Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v12i1.1029

Abstract

Burung kuntul besar (Egretta alba) merupakan burung air yang hidup di ekosistem lahan basah dan berpotensi menjadi reservoir bakteri enterik, salah satunya Enterobacter sp. Bakteri ini bersifat oportunistik dan dapat menimbulkan resiko kesehatan bagi hewan, manusia serta lingkungan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Enterobacter sp. pada burung kuntul besar (Egretta alba) yang hidup di ekosistem hutan mangrove Kecamatan Baitussalam, Aceh Besar. Penelitian ini dilakukan secara observasi lapangan dan eksplorasi laboratorium menggunakan metode Carter. Sebanyak 10 sampel swab kloaka burung kuntul besar diinokulasikan ke dalam media Nutrient Broth (NB), kemudian diisolasi menggunakan media MacConkey Agar (MCA). Koloni yang tumbuh selanjutnya diamati morfologinya, dilakukan pewarnaan Gram, serta diuji secara biokimia meliputi uji IMViC, TSIA, SIM, dan fermentasi gula-gula. Hasil penelitian menunjukkan bahwa seluruh sampel memperlihatkan koloni berwarna merah muda pada media MCA. Secara pewarnaan Gram menunjukkan bakteri yang terisolasi merupakan Gram negatif berbentuk basil. Uji biokimia menunjukkan hasil indol negatif, motil, MR positif, VP negatif, SCA positif, serta mampu memfermentasi berbagai gula. Berdasarkan hasil identifikasi, bakteri yang ditemukan merupakan Enterobacter sp
Efektifitas ekstrak daun nenas (Ananas cosmosus) terhadap penetasan telur ikan bandeng (Chanos chanos Forskall) Muhammad Walidin; Masda Admi
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 2 No 2: November 2020
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v2i2.387

Abstract

Pemijahan mengasilkan telur-telur ikan yang kemudian ditetaskan untuk menghasilkan benih. Jamur sering dijumpai menyerang telur ikan, jamur akan membalut telur ikan dengan ciri-ciri seperti benang dan telur tidak akan menetas dan kemudian membusuk. Daun nenas memiliki senyawa yang sama dengan kulit buah nenas yaitu flavonoid, folifenol, alkaloid dan fenol yang berfungsi sebagai antibakteri dan antimikroba tetapi dengan jumlah berbeda. Penggunaan ekstrak daun nenas dapat mencegah dan membunuh mikroba yang menyerang telur ikan. Penelitian telah dilakukan pada bulan Januari 2016 dan Bulan Februari 2017 menggunakan metode eksperimental sebanyak 3 perlakuaan dosis ekstraksi daun nenas dengan masing-masing 3 ulangan. Perlakuan dosis yaang diberikan adalah 0 ml/ 5 liter air, 20 ml/ 5 liter air dan 30 ml/ 5 liter air. Hasil penelitian menunjukkan penggunaan ekstrak daun Nenas dengan konsentrasi 30 ml/ 5 liter meningkatkan persentase daya tetas sebesar 77%, menurunkan tingkat abnormalitas menjadi 8,95% dan meningkatkan tingkat kelangsungan hidup larva ikan bandeng sebesar 87,87%. Hasil terbaik terdapat pda perlakuan 30 ml/ 5 liter air.
Molecular analysis of the 16S rRNA Gene for the identification of Shigella sp. in freshwater snail (Faunus ater) Masda Admi; Veronika Puja Koswari; Winaruddin Winaruddin; Wahyu Eka Sari; Teuku Zahrial Helmi; Azhari Azhari; Faisal Jamin
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 7 No 1: May 2025
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v7i1.3328

Abstract

Freshwater snails (Faunus ater) live in shallow, muddy, and sandy waters and obtain their food through a filter-feeding mechanism. These conditions make freshwater snails potentially exposed to various contaminants within their bodies, such as heavy metals and bacteria. This study aims to identify the presence of Shigella sp. in Faunus ater using 16S rRNA gene analysis. The research method was conducted through observational and laboratory exploration using a modified Carter method and 16S rRNA gene analysis. Phenotypic observations showed bacterial growth in Brain Heart Infusion Broth (BHIB) media, producing clear, round-shaped colonies on Salmonella Shigella Agar (SSA) media, and were classified as Gram-negative, short rod-shaped bacteria. The 16S rRNA gene analysis results showed that the isolate had a 98.35% similarity to Shigella flexneri strain FDAARGOS_692. It can be concluded that the molecular analysis of the 16S rRNA gene of the bacterial isolate from the freshwater snail indicates that the bacteria belong to the genus Shigella and are similar to Shigella flexneri strain FDAARGOS_692.
Co-Authors . Nurliana Abdul Harris Abdullah Hamzah, Abdullah Abrar, Mahdi Abrar Ali Makmur Amalia Sutriana Amiruddin Amiruddin Annisa Anwar Sitorus Awaluddin Awaluddin Azhari Azhari Azhari Azhari Azhari Daniel, Daniel Daniel Darmawi Darmawi Darmawi Darmawi Darniati Darniati Dasrul Dasrul Erina Erina Erina Erina Faisal Jamin Faisal Jamin Faisal Mustafa Fajar Fakri Fajri, Fauzan Fakhrurrazi Fakhrurrazi Fakhrurrazi Fitri, Khairani Gholib Gholib, Gholib Ginta Riady Hemelikova, Adela Hennivanda Hennivanda Hennivanda, Hennivanda Herrialfian Herrialfian Ismail Ismail Kamaruzaman, Intan Noor Aina Kurniawan, Wilda Lestari, Rafika M Daud AK M Isa M Isa M.iSA - Mahdi Abrar Maryulia Dewi Maryulia Dewi Maulida, Rafdhayatul Muhammad Hambal Muhammad Walidin Mulyadi Adam, Mulyadi Muslim Akmal Mutia Sukma Langkana Nanda Yulian Syah Nora Usrina NURLIANA NURLIANA Nurzalika, Siti putri, anggun bellia Rasmaidar Rasmaidar Rastina, Rastina Razali Daud Rinidar Rinidar Rosa, Teuku Shaddiq rosalia rosalia Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Sari, Wahyu Eka Siti Rani Ayuti Siti Rani Ayuti Sugito Sugito Syafruddin Syafruddin T Zahrial Helmi T Zahrial Helmi T. Fadrial Karmil Teuku Reza Ferasyi Teuku Zahrial Helmi Teuku Zahrial Helmi Triva Murtina Lubis Ummu Balqis Veronika Puja Koswari wahyu eka sari Wardhani, Lailia Dwi Kusuma Widya Nur Hidayati Winaruddin Winaruddin Yuni Sari Yusrizal Akmal Zahwa Amelia Pratiwi Zainuddin Zainuddin Zainuddin, Zainuddin Zulfitri Zulfitri, Zulfitri Zulkifli, Baidillah