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Titik Nuryastuti
Departemen Mikrobiologi, Fakultas Kedokteran, Kesehatan Masyarakat Dan Keperawatan (FKKMK), Universitas Gadjah Mada
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Journal : indonesian journal of laboratory

 1 
GAMICBANK: Pendekatan Inovatif dalam Pelestarian Isolat Mikroba Mulyani, Mulyani; Awaludin, Kamla; Rahayu, Ayu; Nuryastuti, Titik
Indonesian Journal of Laboratory Vol 9, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijl.v9i1.112540

Abstract

ABSTRAK. Pelestarian isolat mikroba sangat penting untuk penelitian, diagnostik, dan bioteknologi. Metode konvensional seperti stok gliserol atau liofilisasi efektif namun seringkali mahal dan rentan terhadap risiko kontaminasi. Strategi alternatif penyimpanan berbasis butiran, memungkinkan pengambilan mikroorganisme yang masih hidup dengan mudah sambil meminimalkan siklus pembekuan-pencairan berulang. Studi ini bertujuan untuk mengevaluasi kinerja awal sistem GAMICBANK, metode penyimpanan mikroba berbasis butiran menggunakan butiran mikrobank plastik yang dimasukkan dalam larutan susu skim atau Skim Milk–Tryptone–Glucose–Glycerol (STGG), untuk menyimpan bakteri dan ragi pada suhu –80 °C. Isolat Gram-positif, Gram-negatif, dan ragi yang representatif (Staphylococcus aureus, Streptococcus spp., Listeria monocytogenes, Salmonella typhi, Shigella flexneri, Escherichia coli, Neisseria gonorrhoeae, Chromobacterium violaceum, dan Candida albicans) disimpan dalam butiran dengan susu skim atau STGG. Butiran tersebut diambil kembali setelah 1 dan 3 bulan, ditanam pada media selektif, dan dievaluasi pertumbuhan koloni dan viabilitasnya. Setelah 1 bulan, semua isolat tetap memiliki viabilitas yang sangat baik di kedua media. Pada 3 bulan, sebagian besar spesies tetap sangat viabel (>99%) di kedua media. Namun, Listeria monocytogenes dan Neisseria gonorrhoeae menunjukkan penurunan viabilitas yang signifikan dalam susu skim (30%) tetapi tetap stabil dalam STGG (99%). Demikian pula, Candida albicans menunjukkan penurunan viabilitas dalam susu skim (50%) tetapi mempertahankan 99% kelangsungan hidup dalam STGG. Sistem bead GAMICBANK secara efektif melestarikan mikroorganisme yang tangguh dan sensitif pada suhu –80 °C. STGG memberikan perlindungan beku yang lebih unggul dibandingkan susu skim, terutama untuk bakteri sensitif dan ragi. Kesimpulannya bahwa sistem GAMICBANK dapat dijadikan sebagai metode alternatif untuk penyimpanan isolat bakteri dan ragi.ABSTRACK.The preservation of microbial isolates is crucial for research, diagnostics, and biotechnology. Conventional methods, such as glycerol stocks or lyophilization, are effective but frequently expensive and susceptible to contamination risks. An alternative strategy, bead-based storage, facilitates the easy retrieval of viable microorganisms while minimizing repeated freeze-thaw cycles. This study aims to evaluate the initial performance of the GAMICBANK system, a bead-based microbial storage method utilizing plastic microbank beads submerged in either skim milk or Skim Milk–Tryptone–Glucose–Glycerol (STGG) solutions, for the cryopreservation of bacteria and yeast at –80 °C. Representative Gram-positive, Gram-negative, and yeast isolates (Staphylococcus aureus, Streptococcus spp., Listeria monocytogenes, Salmonella typhi, Shigella flexneri, Escherichia coli, Neisseria gonorrhoeae, Chromobacterium violaceum, and Candida albicans) were preserved in beads using either skim milk or STGG. The beads were retrieved after 1 and 3 months of storage, cultured on selective media, and evaluated for colony growth and viability. After 1 month, all isolates maintained excellent viability in both preservation media. At 3 months, the majority of the species remained highly viable (>99%) in both media. However, Listeria monocytogenes and Neisseria gonorrhoeae exhibited a significant decline in viability when stored in skim milk (30%) but remained stable in STGG (99%). Similarly, Candida albicans demonstrated a reduction in viability in skim milk (50%) while maintaining a 99% survival rate in STGG. The GAMICBANK bead system effectively preserves both robust and sensitive microorganisms at –80 °C. STGG provides superior cryoprotection compared to skim milk, particularly for sensitive bacteria and yeast. In conclusion, the GAMICBANK system serves as a viable alternative method for the preservation of bacterial and yeast isolates. 
Co-Authors . Mursiti Abu Tholib Aman Ade Christanti Putri Sidabutar Afdina Melya Ganes Febiyanti Aflifia Birruni Sabila Aisyah Nur Sapriati Altaufik Ngani Amaia, Devika N. Amalia Setyati Aman, Alia Hanifa Aminy, Sayyidah A. Andi Ameilia Sari Riandika Anggraeni, Nuniek Annisa Aulia Savitri Annisa Somaningtyas Anton Pratama Apriyanto, Muchammad Arief Nurrochmad Arta Farmawati As, Musdalifa Asysyifa, Azzahra Atikana, Akhirta Atthobari Avanilla Fany Septyasari Awaludin, Kamla Ayu Rahayu Bastiaan P. Krom, Bastiaan Bastian P. Krom Cicilia Widhi Astuti Cindy Elvionita Daniwijaya, Edwin Widyanto Daru Estiningsih Dessy Kurnia Sari Devi Artami Susetiati Dewi Anggraini Dewi Purbaningsih Dhannia Fitratiara Diah Susanti Dicky Moch Rizal Dicky Moch Rizal Diyan Ajeng Rossetyowati Diyan Ajeng Rossetyowati Djoko Wahyono Djoko Wahyono Domas Fitria Widyasari Dwi Aris Agung Nugrahaningsih Dwi Cahyani Ratna Sari Dwi Utami Anjarwati Dwikisworo Setyowireni E. Elsa Herdiana Murhandarwati Edwin W. Daniwijaya Eggi Arguni Elya Antariksana Bachmida Emi Rusdiyati Endang Estriningsih Ervina Damayanti Estiningsih, Daru Estriningsih, Endang Eti Nurwening Sholikhah Firdhani Satia Primasari Fita Rahmawati Fita Rahmawati Galang Ridha Allatief Hakim, Mohamad Saifudin Hamzah, Hasyrul Hananun Zharfa Hanifah Handi Virawan Hanggoro Tri Rinonce Hardyanto Soebono Henk J. Busscher Henk J. Busscher, Henk J. Henny C. van der Mei Henny C. van der Mei, Henny C. Hera Nirwati Ika Puspita Sari Ika Puspita Sari IKA PUSPITA SARI Ika Puspita Sari Ika Puspita Sari Ika Puspita Sari Ika Puspita Sari Ika Puspitasari Ika Puspitasari Ika Puspitasari Ika Puspitasari Ilma Tazkiya Iman Iman Ishak SKM., MPH IWAN DWIPRAHASTO Jaya, Sudi Indra Kholidah, Siti Nurhayati Kris Kurniawan Kuwat Triyana LINDA SUKMARINI Linda, Vitia Ajeng Nur Lucky Herawati Luthvia Annisa Mansyar, Putri Purnama Sari Marselinus Edwin Widyanto Daniwijaya Maulidia, Faiqoh Nur Maulidiah, Rizka Mawarti, Yuli Meilia Nhadia Amalia Mubasysyir Hasanbasri Mujahidah, Mujahidah Mukhriani, Mukhriani Mulyani Mulyani Mursiti, Mustofa, Handry Darussalam, Titik Nuryastuti, Mursiti, Mustofa Mustofa Nanang Munif Yasin Ni Luh Putu Vidya Paramita Niken Puspitasari Ning Rintiswati Nunung Yuniarti Nunung Yuniarti Nurkhalika, Rachmi Nurpagino, Bombong Nurrahma, Bira Arumndari Prameshwara, Almarissa Ajeng Praseno Praseno Pratama, Anton Prawarni, Vidyadhari Puspa Prayoga, Muhammad Bagas Primasari, Firdhani Satia Pujilestari, Dwi Purnomo, Galih Santo PUSPITA LISDIYANTI Qonita Imma Irfani Rachma Dewi Isnaini Putri Rahmawati, Fita Rakhmat Ari Wibowo Rebriarina Hapsari Retno Wahyuningrum Rina Susilowati Rina Triasih Ritmaleni, Ritmaleni Rizal Fauzi Rizka Humardewayanti Asdie Rizka Humardewayanti Asdie Rizqi Nurul Khasanah Roel Kuijer Roel Kuijer, Roel SAMUEL BUDI Sapriati, Aisyah Nur Setiawati, Setiawati SHANTI RATNAKOMALA Subagus Wahyuono Supriyati Susi Iravati Syarifa, Cut Alima Sylvia Utami Tunjung Pratiwi Takushi Kaneko Tatang Irianti Teguh Triyono Tina Amnah Ningsih Titi Ira Pangestuti Tri Murti Andayani Tri Murti Andayani Tri Ratnaningsih Tri Wibawa Triana Hertiani Tristina Devi Azzahra Untari, Febriana Vera Olfiana Wahyuddin, Munifah Widodo, Irianiwati Wihda Yanuar Wihda Yanuar Yanuar, Wihda Yeli Trimayanti Yolanda Pitra Kusumadewi Yusrizal Djam’an Saleh ZULLIES IKAWATI