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Riskawati Riskawati, Riskawati
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Journal : Jurnal Biologi Tropis

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Storage Stability Analysis of Crude Amylase Extract from Banana Peel by Solid State Fermentation Wakiah, Nurul; Fadillah, Ummu Farah; Sudirman, Sudirman; Riskawati, Riskawati
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9855

Abstract

Banana peel is a carbohydrate-rich by-product with potential as a fermentation substrate for enzyme production. This study aimed to evaluate the amylase production and storage stability of enzymes obtained through solid-state fermentation (SSF) of banana peel flour using Bacillus subtilis and Bacillus licheniformis. Fermentation was conducted for 24 and 48 hours, followed by crude enzyme extraction and storage for 24 hours at room temperature. Proximate analysis revealed banana peel flour contained 78.64% carbohydrates and 46.24% starch, supporting its suitability as a fermentation medium. The highest amylase activities were 50.36 IU/mL/min (B. subtilis) and 56.33 IU/mL/min (B. licheniformis) after 24 hours, with comparable values at 48 hours. However, enzyme activity declined by over 78% after 24 hours of storage, indicating low stability at room temperature. These findings confirm that banana peel flour is an effective, low-cost substrate for amylase production via SSF. Nevertheless, stabilization strategies post-fermentation are crucial to preserve enzyme activity during storage. This study highlights the dual benefit of converting agricultural waste into valuable enzymes and supports further research on improving enzyme shelf-life for industrial applications.
Co-Authors Abdurahman Abdurrahman Aco, Putri Mawa Sari Adindi, Satria Agus, Nurfadillah Ahmad Afif Ainun Jariyah Akhfar, Mariani Amir Lukum, Amir Ana Dhiqfaini Sultan, Ana Dhiqfaini Andriani, Miftakul Anti Kinas, Arni Arief Fitrah Istiyanto Aslim, Muhammad Auliyani, Siti Baharuddin , Hendra Baharuddin, Hendra Baso Akib Budiarti Putri Uleng Damayanti Damayanti Dewi Hikmah Marisda Eli Ermawati Eliyah Acantha Manapa Sampetoding Fadillah, Ummu Farah Fajri, Nofias Fakhrimantho, Agus Fatihanursari, Fatihanursari Fitrah, Andi Nurul Gafur, Muzna Ardin Abdul Hadijah Hadijah Handayani, Yuni Hardianti, Fatimah Harmonika, Sri Hasin, Ardiansah Hasnah Natsir Hendra Hendra Herman, Nurul Muthmainnah Hilmi Najamudin, Muhammad Hudriati, Andi Humaira MS, A. Musfira Dwi Idriani, Reski Idris, Ihfa Indira Nurnaifah Iin, Iin Jafar, Agustina Jalil, Erniwati Juliana, Ana Junianti, Fitri Kaso Sanusi, Dirgah Khaeruddin Khaeruddin Kurnia, Fira Lestari, Rianti Indah Lisalohit, Sulaiman Lubis, Deli Anggraeni Made Diah Ratna Dewi Mahmudah, Zuhrotul Malibela, Yulia Katrina Mappeasse, Muh Yusuf Mardiana, Lina Marhani Marhani MARIA VERONIKA ROESMININGSIH Maruapey, Ajang Maswati Baharuddin Mayor, Felia Imelda Mega, Bayu Lesta Melati, Amelia Puspita Mohammad Arief Nur Wahyudien Muh. Khalifah Mustami MUHAMMAD HASBI Muhammad Ikhsan Muhammad Irham Muhammad Kasran Muliana, Nur Munawarah Munawarah, Munawarah Mutahharah Hasyim, Mutahharah Ni Luh Saras Santi Nock M, Jesika Noviyanti Noviyanti, Noviyanti Nur Khaerani Busri Nur Samsi, Andi Nurazmi, Nurazmi Nurbaedah, Nurbaedah Nurlina Nurlina Nursam Nursam NURUL ANNISA Nurwijayanti Padliani, Iin Pani, Rahmayatul Putri, Astrina Wulan Raba Natahanie Rahmawati, Rahmawati Ratnawati Ratnawati Rawi, Rais Dera Pua Rezki Hamzah, Ria Rezki natsir, Andi Dian Sry Rizki Rinaldi, Ade Rombe, Wanti Rosalina, Febrianti Salwa Rufaida Sangadji, Zulkarnain Satriani Satriani Seniwati Dali Sindi, Sindi Sri Agustini Sri Rosita Sri Wulan, Sri Sudirman Sudirman Sukmawati Sukmawati Sulaiman, Lisaholit SULFIANA, SULFIANA Suparni Sampetan, Suparni Susi Ekawati Taqwin, Muhammad Thahir, Nur Fadillah Tjandi, Yunus Tri Handayani Amaliah Wakiah, Nurul Wardan, Kharisma Dewi Sukma Wardana, Anita Watie, Ritha Hima Widiasih, Widiasih Winarti, Widya Yulianti Yusal, Yulianti Yusri Handayani Yusri Yusri Zaenab, Syarifah Zakariah, Mohd Ihwan Bin