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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Majalah Kedokteran Bandung Medical Journal of Indonesia Jurnal Biosains Pascasarjana JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) MPI (Media Pharmaceutica Indonesiana) Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Teknologi Laboratorium Berkala Arkeologi SANGKHAKALA Journal Of Vocational Health Studies Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Berkala Arkeologi Indonesian Journal of Chemistry Meditory : The Journal of Medical Laboratory Poltekita : Jurnal Ilmu Kesehatan Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Ilmu Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Cendekia : Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology International Islamic Medical Journal Jurnal Hukum Kesehatan Indonesia Scolae: Journal of Pedagogy Innovative: Journal Of Social Science Research Ranah Research : Journal of Multidisciplinary Research and Development Sriwijaya Journal of Forensic and Medicolegal Jurnal Forensik dan Medikolegal Indonesia Berkala Arkeologi Tasyri' : Jurnal Muamalah dan Ekonomi Syariah Prosiding International Conference on Sustainable Innovation (ICoSI) Berkala Ilmiah Kedokteran dan Kesehatan Masyarakat Jurnal of Syiah Kuala Dentistry Society Folia Medica Indonesiana
Ahmad Yudianto
Department Of Forensic And Medicolegal Medicine, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia
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VARIASI GENETIK LOCI STR CODIS (THO1,TPOX) MANUSIA GILIMANUK (PULAU BALI) Toetik Koesbardiati; Ahmad Yudianto; Delta Bayu Murti; Rusyad Adi Suriyanto
Berkala Arkeologi Vol. 33 No. 2 (2013)
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30883/jba.v33i2.11

Abstract

It is assumed that Mongoloid’s migration came from western and northern part of Indonesia in various waves of migration. The migrant population then mixed with initial inhabitants, which are Australomelanesoid. The wave of migration moved further to the eastern Indonesia and mixed with migrant that entered from east (Papua). Some researches show that the concentration of mixture (hybridization) of migration was around Wallace’s line. Gilimanuk is one of prehistoric site that yields Neolithic human remains. It is assumed that Gilimanuk can give worthy information about human variation at that time. The aim of the research is to describe the human genetic variation at site of Gilimanuk. The material is DNA (deoxyribonucleic acid) has been extracted from many piece of bone of Gilimanuk’s human remains. We used STR (short tandem repeat) two loci (THO1 and TPOX) to gain human genetic variation. The result show all of sample yields band with different allele. This evidence confirms that they have a genetic affinity is not the same, or their genes from several population.
Determination of ABO blood group based on secretors or non-secretors analysis in body fluids Fery Setiawan; Ahmad Yudianto; Arif Rahman Nurdianto; Jenny Sunariani; Latief Mooduto
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 8 No 02 (2024): Qanun Medika Vol 08 No 02 2024
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v8i02.20561

Abstract

Criminal acts often leave traces analyzed by law enforcement, especially by the Indonesian National Police (POLRI). Sometimes, various items were found and analyzed to link the victim and the perpetrator. Blood, the most abundant body fluid, found in crime scenes, consists of cellular and liquid elements. The forensic focuses on red blood, which can be wet or dried at the crime scene. Sometimes, there was no blood or bloodstains found, but objects related to other body fluids, such as: condoms, toothbrushes, the mouth of glass cups, straws, etc, could be found. Blood group analysis could still be carried out based on secretor gene (Se) considering that 80% of the population was of the secretor group. There are two chromosomes related to blood regulation, namely: chromosomes no. 9 and 19. The former relates to the ABO blood group and the latter relates to the secretory phenotype. The basic principle of secretors was antigens found on the surface of red blood cells and all body fluids, except cerebrospinal fluid. The pattern of inheritance on chromosomes 9 and 19 is based on the probability law by Mendell. It stated that all of genotypes (homozygous dominant-recessive or heterozygous) would be passed on to their offspring.
Forensic Autopsy of Multiple Stab Wound Perwira, Satria; Prasilia Ramadhani; Edwin Tambunan; Ahmad Yudianto
Sriwijaya Journal of Forensic and Medicolegal Vol. 2 No. 1 (2024): Sriwijaya Journal of Forensic and Medicolegal
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/sjfm.v2i1.103

Abstract

Introduction: Stabbing is the predominant form of homicidal violence globally, resulting in both physical impairment and death. A stab wound is the result of a forceful push from a sharp or blunt pointed object, resulting in a wound that is deeper than it is length. Case presentation: We present a case of a man who was found dead after being stabbed with a sharp weapon by his partner in a public area, with an open wound on the left chest. A forensic expert identified the body at Dr. Soetomo General Hospital, Surabaya, Indonesia. On external examination, we found 23 open wounds on several anatomical regions. Vital injuries include an open wound on the right side of the neck and the back of both sides of the chest. In an internal examination, we found both lungs looked shrunken, and blood was found in the right and left chest cavities. The stab wound to the right back penetrated the right lower lung and right liver lobe. The stab wound to the left back penetrated the lower left lung. Conclusion: A complete autopsy is vital to determine the cause, mechanism, and manner of death and to reconstruct the events before death.
THE USE OF KINSHIP ANALYSIS ON PATERNITY TESTING THROUGH CODIS STR LOCI ‘CSF1PO' AND ‘THO1' Putri, Dwi Fitrianti Arieza; Yudianto, Ahmad
Biomolecular and Health Science Journal Vol. 2 No. 2 (2019): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.68 KB) | DOI: 10.20473/bhsj.v2i2.15793

Abstract

Introduction: Paternity tests compare a child's DNA pattern with the possible father to examine the DNA heritage in ensuring kinship. If there is no information from the father and mother or the child that can be used as a comparison in the forensic DNA examination process (paternity test), there must be a comparison from a close relative as an alternative to obtain the forensic DNA examination. This experiment's purpose is to analyze the use of kinship analysis in forensic identification especially in a paternity test. Methods: This is a descriptive experiment using a cross-sectional design through locus analysis of DNA forensic examination in paternity test using the kinship analysis through STR CODIS loci: CSF1PO and THO1. The variables of this experiment were ‘locus' as the independent variable and ‘allele number' as the dependent variable. This experiment conducted a paternity tests to 8 samples in which the subjects were siblings; the test was administered on the CSF1PO and TH01 loci. Results: This experiment displayed similar allele numbers on the same locus, both in 50% and 100% allele numbers; the research showed similarity in allele numbers of both siblings of which ¼ were inherited  from the parents (for 50% allele number similarity), and ½ were inherited  from the parents (for 100% allele number similarity). Conclusion: This proves that a paternity test using siblings as the closest kin (kinship analysis) can be used as an alternative if no comparison is obtained from both the parents.
Clinical Forensic Examination on Mass Violence’s Victim With Head and Ocular Injury (A Case Report) Syarifah, Mustika Chasanatusy; Yudianto, Ahmad
Jurnal Medis Islam Internasional Vol 1 No 1 (2019): December
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/iimj.v1i1.1389

Abstract

Introduction: Clinical forensic medicine is a branch of medicine that applies medicine to uphold justice in the case of living victims. In accordance with this understanding, the handling of cases of living victims, especially victims of crime, must involve doctors in the field of forensics, in addition to doctors in other fields according to their competence. With so many cases of violent crime, a doctor must be able to provide a report on the results of the examination to the legal apparatus to assist in the judicial process. Case: A man, aged 44 years, found bruises on right eyelid, left eye, left cheek, left and right scalp; blisters on the left and right eye membranes; a cut wound on the right eye membrane and the left eyelid; bleeding under the thick membrane of the brain and swelling of the brain. Discussion: Injuries can occur as a result of violence perpetrated by criminal offenses, such as cases of theft by violence, murder, traffic accidents or ill-treatment. The doctor must be able to mention that the wound examined is caused by sharp violence, blunt force, electrical trauma, thermal trauma, chemical trauma, or other causes of trauma. Determination of the magnitude of the effect of injury to the victim, related to criminal sanctions that will be imposed on the perpetrators of criminal acts. The head is an organ that is often the target of violent blunt crimes. The brain and the surrounding layers are easily susceptible to trauma, resulting in death compared to other body parts. The wound is in accordance with Criminal Code article 90 concerning serious injuries, which results in injuries that do not give any hope of healing at all.
Clinical Forensic Assessment of Victims with Stab Wound Prabowo, Yudha Erik; Suryanegara, I Ketut Heru; Perwira, Satria; Yudianto, Ahmad
Jurnal Medis Islam Internasional Vol 6 No 1 (2024): June
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/iimj.v6i1.5898

Abstract

Introduction: Violent crimes using sharp objects are a common problem in Indonesia because sharp weapons can be easily accessed. When evaluating sharp-force injuries, the correct interpretation of the morphology of individual wounds, as well as the overall pattern of findings, is of paramount importance in the reconstruction aspect of the crime. Any knowledge of the occurrence and distribution of sharp force injuries in knife attack victims and perpetrators can help to verify statements or expose defensive lies. Case: We present, a patient from another hospital came to the emergency room at Doctor Soetomo Hospital with a stab wound to the right chest. The 35-year-old male victim, with the initials SPJ, admitted that he was stabbed by his brother. The victim's vital signs were stable when examined. On the right chest, an open wound with flat edges and sharp and blunt angles was found, measuring three centimeters long and five centimeters wide. The victim underwent radiological and laboratory examinations, installed water-sealed drainage, and was administered anti-pain and antibiotics.  Discussion: The qualification for this patient's wound based on the Indonesian Criminal Code and the scoring carried out is a moderate wound Conclusions: Based on the law and the scoring carried out by the author, the wounds on the patient's body are injuries resulting from a criminal act and are included in the category of moderate abuse, which means that the injuries that occurred to the victim did not cause death or cause permanent disability but caused temporary obstacles in carrying out work.
VALIDATION OF HEADSPACE SOLID-PHASE MICROEXTRACTION WITH GAS CHROMATOGRAPHY-FLAME IONISATION DETECTOR METHOD FOR ALCOHOL ANALYSIS ON GASTRIC FLUID Thaiyibah, Nazaratun; Yuwono, Mochammad; Yudianto, Ahmad
Jurnal Kimia Riset Vol. 8 No. 2 (2023): December
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v8i2.44502

Abstract

Identifying the concentration of alcohol compounds in postmortem analysis of biological fluids can help the investigation, just as postmortem analysis of gastric juices can reveal the concentrations of alcohol consumed. However, an efficient and effective combination of extraction and measurement methods is required when looking at complex postmortem samples. Therefore, a headspace-solid phase microextraction (HS-SPME) method was developed using gas chromatography with a flame ionization detector (GC-FID) to identify postmortem alcohol concentrations. This present study optimizes and validates an effective and efficient method for postmortem alcohol extraction and quantification in gastric fluid. The optimal conditions for HS-SPME extraction using 65μm Polydimethy lsiloxane Divinyl benzene (PDMS/DVB) fiber for analyte isolation were 15 minutes. at 60°C and an exposure time of 1 minute. The validation investigation shows that the suggested approach satisfies the criteria for linearity, precision, accuracy, LOD, and LOQ for postmortem measurement of alcohol in gastric fluid.
Trauma Profile of Homicide Victims at Dr. Soetomo General Academic Hospital, Surabaya, during the COVID-19 Pandemic Syarif, Ichsan; Yudianto, Ahmad; Saleh, Tania Ardiani; Sulistyorini, Nily
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 15 No. 1 (2024): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V15I12024.17-22

Abstract

Highlights: Most homicide victims were predominantly in the productive age range of 21-40 years old. Males were more likely to be homicide victims than females.   Abstract Introduction: The injuries of homicide victims are clues in forensic medicine to uncover a possible crime. In Indonesia, there are not many studies on trauma profiles, specifically in homicide cases. This study aimed to provide an overview of the trauma profile of homicide victims at Dr. Soetomo General Academic Hospital, Surabaya, from January 2020 until December 2021. Methods: This was a descriptive study. Secondary data was collected from 34 forensic examination reports at Dr. Soetomo General Academic Hospital, Surabaya. Data such as age, sex, type of injury, sites of injury, number of injuries, cause of death, and mechanism of death were analyzed statistically. Results: Most victims were between 21 and 30 years old and 31 and 40 years old (26.47%). Sex was dominated by men (76.47%). The most common type of injury was abrasion (25.71%). The most common injury sites were head-neck (34.34%) and chest-upper back (34.34%). The most common number of injuries was ≥10 (44.12%). The most common cause of death was sharp-force trauma (54.54%), and the most common mechanism of death was exsanguination (54.54%). Conclusion: Males predominated in the age ranges of 21 to 30 years old and 31 to 40 years old. The most common type of injury was abrasion. The most common sites of injury were the head, neck, and chest upper back. The most common number of injuries was ≥10. Most victims died of sharp-force trauma. Exsanguination was the most common mechanism of death.
Limit Detection of Short Tandem Repeats (STR) Analysis on Touch DNA Samples Saamia, Vira; Yudianto, Ahmad; Nurjayadi, Muktiningsih; Novitasari, Novitasari; Furqoni, Abdul Hadi
Indonesian Journal of Chemistry Vol 24, No 5 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.94081

Abstract

Forensic short tandem repeats (STR) profiling on touch DNA samples has emerged as a primary method for human identification. The stability and uniqueness of STR combination from the targeted locus in each individual make it a precision marker for human identification. Touch DNA samples can be found in traces of biological material shed from a person. This work aimed to identify the lowest concentration limit required for generating an interpretable DNA profile and the sensitivity of the STR loci applied. Touch DNA samples were collected from donors who were asked to hold a rope for 5 min. A double swab technique was used to lift the touch samples from the rope. These samples are subjected to DNA extraction and quantification. Two STR amplification cycles, 29 and 34 cycles, were used. DNA concentration greatly influences the success of amplifying the target allele at each STR locus to be interpreted into a complete DNA profile, shown by its allele peak. Touch DNA concentration >0.25 ng can produce a complete DNA profile. LCN method successfully amplified touch DNA with a concentration 0.0625–0.25 ng/µL. Limit detection of touch DNA analysis is 0.25 ng/µL. Low-copy DNA can still be analyzed within 0.0625–0.25 ng/µL.
EFFECT OF POST MORTEM INTERVAL TOWARD Î’-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS Susilo, Imam; Putra, Bilqisthi Ari; Yudianto, Ahmad
Journal of Vocational Health Studies Vol. 7 No. 2 (2023): November 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V7.I2.2023.107-114

Abstract

Background: The effects of post mortem interval on tryptase levels are unclear and have been challenging to quantify due to limited and at times contradictory research. Purpose: Analyze the effects of Post Mortem Interval (PMI) due to anaphylactic shock on the expression of β-tryptase and mast cell chymase in the heart organ. Method: This type of research was an experimental study with a randomized block design (RBD) with the same subjects and a time series for 24 hours, using 5 (five) rabbits. The measurement of β-tryptase and chymase expression was based on immunohistochemical IRS (immunoreactive score) in the heart organs of rabbits experiencing anaphylactic shock sensitization and induction of anaphylactic shock using ovalbumin. Result: Based on the results of statistical tests using univariate analysis, there was a relationship between the length of death (post mortem interval) and the expression of mast cells β-Tryptase and cardiac chymase (p-value = 0.006) and chymase (p-value = 0.002) with (p-value <0.05). The effective test time with β-tryptase markers using cardiac organs was prone to long death time of 0 - 15 hours. The effective check-up time with chymase markers using the heart organ was at a long time of death of 9 hours. Conclusion: The results of the study showed that tryptase and chymase could be used as markers of anaphylactic shock.
Co-Authors A’yun, Qurrota Achmad Basori, Achmad Acrivida Mega Charisma Agung Sosiawan Amalia Rozaiza Ightikhoma Anak Agung Putu Santiasa Putra Andika Aliviameita Apriliani, Herlina Arfianti, Evy Arif Rahman Nurdianto Arif Rahman Nurdianto Ariyanto Wibowo Ariyanto Wibowo Ariyanto Wibowo, Ariyanto Aung, Htet Htet Azizatul Haq Larasati Biqisthi Ari Putra Cicilia, Cindy Oktavi Deka Bagus Binarsa Delita Bayu Murti Delta Bayu Murti Desy Martha Panjaitan Djaja Surya Atmadja Endarini, Lully Hanni Evan Boedi Dewanto Eveline Yulia Darmadi Fery Setiawan Fery Setiawan Furqoni, Abdul Hadi Heni Puspitasari Heribertus Agustinus B Tena Herkutanto Huda, Qurrota A’yunil Hutagalung, Magda Rosalina Ida Bagus Narmada Imam Susilo Indah Nuraini Indah Nuraini Indah Nuraini Indah Nuraini Masjkur Indah Nuraini Maskjur Indah Nuraini, Indah Januar Alif Jenny Sunariani Jenny Sunariani Jenny Sunariani, Jenny Juliana Christyaningsih Kristianto, Sonny Latief Mooduto, Latief Leonardo Margaret, Nola Masniari Novita Meli Meli, Meli Mely Purnadianti Mieke Sylvia Mochammad Yuwono Muhammad Afiful Jauhani Muhammad Kholil Ikhsan Muktiningsih Nurjayadi Nazaratun Thaiyibah Nazaratun Thaiyibah, Nazaratun Nily Sulistyorini Nola Margaret Nola Margaret NOVITASARI Novitasari Novitasari Nurdianto, Arif Rahman Nurdin, Erni Perwira, Satria Prabowo, Yudha Erik Prasilia Ramadhani Priyatna, Setya Aji Purnamaningsih, Sari Nur Indahty Puspa Wardhani Puspa Wardhani Puspa Wardhani Putra, Bilqisthi Ari Putra, Biqisthi Ari Putri, Dwi Fitrianti Arieza Putri, Rury Erina Qurrota A'yunil Huda Rahma Diyan Martha Reni Sumino Rizal Fauzi Nurdianto Rosalinda Avia Eryatma Rury Eryna Putri Rusyad Adi Suriyanto Rusyad Adi Suryanto RWulandari, Septiayu Saamia, Vira Saleh, Tania Ardiani Setiawati, Rosy Simon Martin Manyanza Nzilibili Sispitasri, Yeti Eka Sri Puji Astuti W Sugiharto, Ade Firmansyah Sulistyorini, Nilly Suryanegara, I Ketut Heru Syarif, Ichsan Tambunan, Edwin M.B. Tiara Mayang Pratiwi Lio Titik Erliyah Toetik Koesbardiati Utomo, Ratno Tri Wahyu Widodo Wahyuningsih, Astuti Wimbuh Tri Widodo Wresti Indriatmi B. Makes wulandari, septiayu Yessy Andriani Fauziah Yeti Eka Sispita Sari Yeti Eka Sispitasri