Article Per Year (5 Year)
p-Index From 2021 - 2026
5.161
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Majalah Kedokteran Bandung Medical Journal of Indonesia Jurnal Biosains Pascasarjana JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) MPI (Media Pharmaceutica Indonesiana) Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Teknologi Laboratorium Berkala Arkeologi SANGKHAKALA Journal Of Vocational Health Studies Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Berkala Arkeologi Indonesian Journal of Chemistry Meditory : The Journal of Medical Laboratory Poltekita : Jurnal Ilmu Kesehatan Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Ilmu Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Cendekia : Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology International Islamic Medical Journal Jurnal Hukum Kesehatan Indonesia Scolae: Journal of Pedagogy Innovative: Journal Of Social Science Research Ranah Research : Journal of Multidisciplinary Research and Development Sriwijaya Journal of Forensic and Medicolegal Jurnal Forensik dan Medikolegal Indonesia Berkala Arkeologi Tasyri' : Jurnal Muamalah dan Ekonomi Syariah Prosiding International Conference on Sustainable Innovation (ICoSI) Berkala Ilmiah Kedokteran dan Kesehatan Masyarakat Jurnal of Syiah Kuala Dentistry Society Folia Medica Indonesiana
Ahmad Yudianto
Department Of Forensic And Medicolegal Medicine, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Transportation Veterinary Other

Published : 70 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 70 Documents
Search

 3   4   5   6   7 
Dna isolation from human urine stain as an alternative material for personal identification examination Yudianto, Ahmad; Sispitasari, Yeti Eka
Folia Medica Indonesiana Vol. 52, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Accurate determination of personal identity is crucial for an investigation since any inaccuracy may lead to fatal consequences in the judicial process. Identification through DNA analysis involves somatic chromosomes and mtDNA. Each part of the human body can be taken as a specimen since every nucleated cell in the body of an individual has identical DNA sequence. To date, samples for identification through DNA analysis are obtained from blood stains, semen stains, bones, vaginal swab, buccal swab etc. In certain cases, urine stains on the clothing have frequently been overlooked. So far, personal identification through DNA analysis by the use of urine stains has not been commonly carried out. The present study detected bands in the loci CSF1PO, THO1, TPOX and 106bp-112bp amelogenin in all samples visualized from the results of Polymerase Chain Reaction (PCR) with Polyacrylamid Agarose Gel Electrophoreses-silver staining for exposure durations of 1, 7 and 14 days. However, for exposure duration of 20 days (the maximum in the study), bands were only detected in the loci THO1 and TPOX in all samples (100%), whereas the loci CSF1PO and 50% amelogenin exhibited obvious bands. This indicated that DNA analysis of urine stains through detection of the locus STR CSF1PO, THO1, TPOX exhibited different detection responses for different exposure durations assigned to the samples of urine stain. Successful detection of these loci was supported by the differences in amplicon product and GC content at each locus. Of the loci studied, the ratio of GC content of the primers, sorted from the lowest, were as follows: locus CSF1PO of 42.6 1%, TPOX of 56.25%, and THO1 of 63.83%. In conclusion, the loci THO1 and TPOX had the same probability of success in the STR examination compared with the locus CSF1PO.
Analysis of earphone swab mitochondrial dna as an alternative material for identification examination Yudianto, Ahmad; Sispitasri, Yeti Eka; Margaret, Nola
Folia Medica Indonesiana Vol. 52, No. 3
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Identification include fingerprint, property, medical, dental, serologic and exclusion methods. In the development, identification methods led to molecular forensics, a new field of science evolving since the 1980s, known as DNA fingerprinting. Specimens widely used in DNA assay for identification are blood spots/bloods, semen spots, vaginal swabs, buccal swabs and bones. In addition to these specimens, the last objects often used by the perpetrators/victims can be used, such as hearing aids (headsets/earphones). In its use, earphones are attached to the outer ear skin; thus, the earwax is suspected to adhere to the device. To date, in Indonesia personal identification is performed through swabs of earphones/headsets using the DNA profiling method. In particular, mitochondrial DNA has not been widely used for identification. The present study was of laboratory experimental. Earphones which have been used for 3 days were placed in room temperature for 1, 7, 14 and 20 days. Results showed that the environmental factor of exposure duration had an effect of a significant decrease in the levels of DNA from day 1 to day 20. Only 126-bp mtDNA (HVS II) was detected on the samples of day 1 and continued with sequencing. Mitochondrial DNA has better durability and relatively higher number of copies than those of nuclear DNA. This leads to greater possibility of success in amplification, given the higher number of mitochondrial DNA copies and the fact that mitochondrial DNA is a single locus that allows recombination.
Forensic Identification of Blood Types in Pear (Pyrus bretschneideri) Fruit Bitemark Aliviameita, Andika; MAR, Mieke Sylvia; Yudianto, Ahmad
Folia Medica Indonesiana Vol. 54, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Blood type in saliva can be examined through bitemarks on an object left at the crime scene in a crime case as a screening test of suspected perpetrators. Saliva deposited at the bite contains glycoproteins which can carry blood type ABH antigens expressed in the salivary glands and excreted in saliva. Salivary examination is influenced by various external factors that damage saliva, one of which is the duration of exposure to room temperature in a certain period of time. This study aimed to determine differences in protein levels and blood group agglutination titers in (Pyrus bretschneideri) pear bitemark saliva during room temperature exposure within 30, 60 and 90 minutes. This type of study was experimental laboratories with time series design. The samples used were 18 pear bitemarks from 6 individuals who had blood groups A, B and AB then incubated for 30, 60 and 90 minutes. Analysis of protein content using trizol reagent was determined spectrophotometrically, while blood group examination used inhibition absorption method. Anova test showed significant difference between groups of 30 minutes exposure duration with 60 minutes and 90 minutes exposure group (P<0.05; 95% confidence interval). The Pearson correlation obtained the results of -0.739, indicating correlation that the longer exposure to room temperature, the lower the protein content. The results showed that there was a decrease in salivary protein levels and blood group agglutination titers. Salivary protein levels were still detected in the time range of 30, 60 and 90 minutes, so that blood type can still be examined from saliva in bitemarks on pear fruit.
Codis str loci (csf1po, thoi, tpox, vwa) genetic variation analysis in madurese Yudianto, Ahmad; Sosiawan, Agung; Margaret, Nola
Folia Medica Indonesiana Vol. 52, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Endogamy continues to occur among the Madurese people in rural areas of the island of Madura, especially those areas of the smallest islands around the mainland of Madura. Endogamy as seen from a genetic standpoint will increase the frequency of homozygote genotypes. With regard to genetic variations, STRs of nuclear DNA and polymorphisms in mtDNA are frequently examined. Genetic variations in human undergo an evolutionary process through the accumulation of changes in DNA sequence, i.e. through the process of nucleotide substitutions that evolves in number with the directional development of lineage. So far, the genetic variations among the populations in Madura Island have not been known. The present study was an observational analytical research with the purpose of determining the genetic variations in STR CODIS in the populations of Madura Island. Results indicated that, based on loci alelle: CSF1PO, THOI, TPOX, and vWA, there was homozygote genotypes. The allele variations is not specific for Madurese ethnic but this variations may represent married model in Madurese ethnic. According to Mustama (2007), a gene pool is not only a collection of genes but a dynamic system organized and containing the past history of a population. Any genetic information has certain historical, anthropological and statistical aspects necessitating an interdisciplinary coordination and collaboration.
Effect of room temperature on the quality of dna from earphone swab by observing mitochondrial dna [mtdna] D-loop region of 126 bp (hvs ii, nt 34-159) and 143 bp (hvs I, nt 16268-16410) Yudianto, Ahmad; Margaret, Nola
Folia Medica Indonesiana Vol. 53, No. 2
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objects contained in crime scene and its surroundings have significance in the examination of forensic identification. The most commonly used specimens in the examination for identification are blood/blood spots, semen patches, vaginal swabs, buccal swabs and bones, including items often used by the perpetrator/victim the last time. For example, a mobile phone hearing aid (headset/earphone). In the use of earphones attached to the outer ear skin so it is suspected there is a serumen attached to the tool. One factor that can affect the quality of DNA is the prolonged exposure. Until now in Indonesia the effect of long exposure to room temperature on the quality of DNA on the DNA material of earphone swabs through DNA analysis has not been widely known. The type of study was laboratory experimental. Used earphones are exposed at room temperature within 1, 7, 14 and 20 days. The results of this study indicate that the environmental effect, ie the duration of exposure, affects the decrease in DNA content significantly from day 1 to 20. Detection of PCR mtDNA D-Loop HVS I visualization results 143 bp nt: 16268 16410 shows positive detection results (+) Only at day 1 exposure to room temperature [4 sample/66,67%] and day 7 [3 sample/50%]. Visualization of PCR results mtDNA D-Loop HVS II 126bp nt: 34 -159 was performed on day 1 day room exposure [2 sample/33,37%] and day 7 [6 sample/100%]. Conclusion, the duration of exposure to room temperature affect the quality of DNA from earpiece swab material. Decreased levels of earphones DNA swabs showed a significance value (p <0.005) against the effect of long time exposure to room temperature.
Expressions of β-Tryptase and Chymase in Lung Mast Cells due to Anaphylactic Shock through Histopathological Appearance at Different Post-Mortem Intervals Putra, Biqisthi Ari; Susilo, Imam; Yudianto, Ahmad
Folia Medica Indonesiana Vol. 59, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights: • The post-mortem interval is related to tryptase and chymase expressions in anaphylactic shock incidence • Forensic experts can utilize tryptase and chymase as markers of anaphylactic (non-anaphylactoid) shock that occurs in the lungs. Abstract: Anaphylactic shock is a hypersensitivity response, a commonly type I hypersensitivity involving immunoglobulin E (IgE). It is caused by an antigen-antibody reaction that occurs immediately after a sensitive antigen enters the circulation. Anaphylactic shock is a clinical manifestation of anaphylaxis that is distributive shock, characterized by hypotension due to sudden blood vessel vasodilation and accompanied by a collapse in blood circulation that can result in death. β-tryptase and mast cell chymase expressions in the lungs of histopathological specimens that had experienced anaphylactic shock were examined at different post-mortem intervals in this study. A completely randomized design (CRD) method was employed by collecting lung samples every three hours within 24 hours of death, and then preparing histopathological and immunohistochemical preparations. The mast cell tryptase and chymase expressions were counted and summed up in each field of view, and the average was calculated to represent each field of view. The univariate analysis yielded p-values of 0.008 at the 15-hour post-mortem interval, and 0.002 at the 12-hour post-mortem interval. It was concluded that tryptase and chymase can be utilized as markers of anaphylactic (non-anaphylactoid) shock in the lungs.
Local Mapping Profile of Mitochondrial DNA (MtDNA)-Loop in Forensic Identification Yudianto, Ahmad; Margaret, Nola
Folia Medica Indonesiana Vol. 54, No. 3
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

To prove that mitochondrial DNA damage is not total or partial, as has been found in the preliminary study, studies need to be done to determine the opportunity of successful use of the mitochondrial DNA mini-primer set in an amplicon product below 250 bp. This is important because it can overcome quality problems in degraded DNA, which will complicate the process of DNA forensic identification. This was an observational analytic study with cross sectional design. The study material was DNA from blood and sweat stains taken from abandoned bodies. Samples consisted of 24 pieces of blood and sweat spots. The measurements of mean DNA levels and sample purity used UV-Visible Spectrophotometer, revealing mean DNA in blood samples of 152.89 ± 85.71 µg/ml and sweat samples of 89.19 ± 5.58 µg/ml, and sample purity of DNA and sweat were 1.89 ± 0.71 and 1.69 ± 0.76. Whereas, the result of D-Loop mtDNA: D-Loop I 143bp nt: 16268 -16410 and D-Loop HVS II 126bp nt: 34 -159, indicating blood spots were detected positively >95% and sweat was detected positively in 5%-20%. Results of DNA sequencing from mtDNA of blood spots and sweat spots in 126 bp and 143 bp amplicon revealed nucleotide damage marked with the letter 'N'. In conclusion, mini-primers of mitochondrial DNA in the amplification product mtDNA D-Loop HVS II 126 bp (nt 59-134) and D-Loop HVS I 143 bp (nt 16268- 16410) were effectively used as support for DNA profiling in forensic medicine.
The Effect of Time on Blood Types of ABO Systems on Saliva Spots of Cigarette Butts for Forensic Identification Purnadianti, Mely; MAR, Mieke Sylvia; Yudianto, Ahmad
Folia Medica Indonesiana Vol. 54, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The identification process is not only carried out on the body of a victim of a crime, but identification can also be carried out on the evidence evidence found at the crime scene. The timing of a crime case and the time interval for collecting evidence of a crime is an obstacle in the process of identifying blood saliva on cigarette butts. Saliva will dry within an hour and forty minutes at room temperature and with the influence of various other factors saliva will dry in less than three hours. The purpose of this study was to determine the effect of the duration of temperature exposure on the protein levels of cigarette saliva in order to help the identification process of forensic blood groups. The time series design was used in this study where 18 filter cigarette butts were collected from 6 individuals who were subjected to research with blood types A, B and AB then incubated 1.3 and 6 hours. Examination of protein content was carried out using trizol reagent with UV spectrophotometer reading. The data was processed using non-parametric T-test statistics. There was a decrease in salivary levels in a predetermined time of 1, 3 and 6 hours. Cigarette butt saliva protein levels can still be detected within 1, 3 and 6 hours so that they can be used to help identify the forensic blood group from cigarette butt saliva.
Acid Phosphatase and Zinc Tests Are Effective for Semen Examination and Identification to Prove Intercourse Yudianto, Ahmad; Wibowo, Ariyanto; Nuraini, Indah; Aung, Htet Htet
Folia Medica Indonesiana Vol. 56, No. 3
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The presence of spermatozoa in vagina is a definite sign of sexual intercourse. However, sometimes microscopic examination does not find spermatozoa or reveals a false negative result. This is influenced by many factors, including the absence of ejaculate in the vaginal canal. In addition, there are other factors such as oligo/azoospermia, vasectomy, degeneration of sperm due to time, incorrect sampling, and improper storage. Therefore, examination of the other components of the ejaculate, ie. the enzyme acid phosphatase, choline and spermin, is important. Compared with spermatozoa, the enzyme phosphatase, choline and spermin have lower evidentiary value because these three components are less specific. However, the level of phosphatase enzyme found in the vagina is much lower than phosphatase enzyme that comes from prostate gland. In this study, as many as 192 samples in the form of patches with sperm/semen stains were tested with acid phosphatase test and zinc test through direct and indirect examination. In the first method, washing was carried out on day 1, day 7, and month 3, and testing was carried out after each washing. In the second method, washing was carried out simultaneously and testing was carried out on day 1, day 7 and month 3. Washing was carried out using 7 types of detergent for each 4 patch samples, and washing using water as control. The results showed very low sensitivity (0.186) and very high specificity (100%). This showed that both tests had high specificity values. Acid phosphatase test specifically showed the presence of the enzyme phosphatase, while zinc test specifically showed the presence of zinc in semen. This phosphohydrolase-phosphatase enzyme is easily degraded due to external factors, including temperature, humidity, and chemicals, ie. the element SDS (Sodium Dodecyl Sulfate) that has the ability to cut enzymes. The weakness of the acid phosphatase test is that this enzyme is easily degraded, either partially or completely, due to external factors, such as temperature, humidity, heat, and the presence of chemicals.
Analisis Temuan Autopsi pada Kasus Kematian Akibat Cedera Otak Traumatik karena Kekerasan Tumpul: Sebuah Laporan Kasus Priyatna, Setya Aji; Purnamaningsih, Sari Nur Indahty; Yudianto, Ahmad
Berkala Ilmiah Kedokteran dan Kesehatan Masyarakat Vol. 4 No. 1 (2026)
Publisher : Fakultas Kedokteran, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar Belakang: Cedera Otak Traumatik (COT) merupakan masalah kesehatan global yang signifikan, khususnya di negara berkembang dengan angka mortalitas yang tinggi pada populasi dewasa muda. Trauma tumpul pada kepala dapat diklasifikasikan berdasarkan tingkat keparahannya dan sering kali berujung pada kematian akibat kerusakan organ vital, perdarahan intrakranial, serta komplikasi sistemik. Identifikasi patologi forensik yang presisi sangat diperlukan untuk menentukan mekanisme kematian pada kasus kekerasan tumpul (blunt force trauma). Deskripsi Kasus: Kami melaporkan kasus kematian seorang laki-laki yang diduga akibat penganiayaan massa. Jenazah dievakuasi ke Instalasi Kedokteran Forensik dan Medikolegal RSUD Dr. Soetomo Surabaya untuk pemeriksaan berdasarkan surat permintaan visum et repertum. Pemeriksaan luar menunjukkan tanda kekerasan tumpul berupa luka lecet pada regio frontal, labial, dan ekstremitas atas, serta luka memar pada area periorbital dan mukosa bibir. Pemeriksaan dalam (autopsi) mengungkapkan adanya resapan darah pada kulit kepala bagian dalam, perdarahan subaraknoid, perdarahan intraventrikular, serta petekie pada parenkim otak. Pemeriksaan histopatologi mengonfirmasi adanya kongesti pembuluh darah yang signifikan pada serebrum dan batang otak. Simpulan: Berdasarkan temuan autopsi dan pemeriksaan penunjang, penyebab kematian disimpulkan sebagai akibat kekerasan tumpul pada kepala yang menyebabkan perdarahan intrakranial masif dan memicu mekanisme asfiksia (mati lemas). Kasus ini menegaskan krusialnya pemeriksaan forensik menyeluruh dalam pembuktian kausalitas kematian pada kasus trauma tumpul. Kata kunci: cedera otak traumatik; kekerasan tumpul; autopsi forensik; perdarahan intrakranial; penganiayaan; asfiksia.
Co-Authors A’yun, Qurrota Achmad Basori, Achmad Acrivida Mega Charisma Agung Sosiawan Amalia Rozaiza Ightikhoma Anak Agung Putu Santiasa Putra Andika Aliviameita Apriliani, Herlina Arfianti, Evy Arif Rahman Nurdianto Arif Rahman Nurdianto Ariyanto Wibowo Ariyanto Wibowo Ariyanto Wibowo, Ariyanto Aung, Htet Htet Azizatul Haq Larasati Biqisthi Ari Putra Cicilia, Cindy Oktavi Deka Bagus Binarsa Delita Bayu Murti Delta Bayu Murti Desy Martha Panjaitan Djaja Surya Atmadja Endarini, Lully Hanni Evan Boedi Dewanto Eveline Yulia Darmadi Fery Setiawan Fery Setiawan Furqoni, Abdul Hadi Heni Puspitasari Heribertus Agustinus B Tena Herkutanto Huda, Qurrota A’yunil Hutagalung, Magda Rosalina Ida Bagus Narmada Imam Susilo Indah Nuraini Indah Nuraini Indah Nuraini Indah Nuraini Masjkur Indah Nuraini Maskjur Indah Nuraini, Indah Januar Alif Jenny Sunariani Jenny Sunariani Jenny Sunariani, Jenny Juliana Christyaningsih Kristianto, Sonny Latief Mooduto, Latief Leonardo Margaret, Nola Masniari Novita Meli Meli, Meli Mely Purnadianti Mieke Sylvia Mochammad Yuwono Muhammad Afiful Jauhani Muhammad Kholil Ikhsan Muktiningsih Nurjayadi Nazaratun Thaiyibah Nazaratun Thaiyibah, Nazaratun Nily Sulistyorini Nola Margaret Nola Margaret NOVITASARI Novitasari Novitasari Nurdianto, Arif Rahman Nurdin, Erni Perwira, Satria Prabowo, Yudha Erik Prasilia Ramadhani Priyatna, Setya Aji Purnamaningsih, Sari Nur Indahty Puspa Wardhani Puspa Wardhani Puspa Wardhani Putra, Bilqisthi Ari Putra, Biqisthi Ari Putri, Dwi Fitrianti Arieza Putri, Rury Erina Qurrota A'yunil Huda Rahma Diyan Martha Reni Sumino Rizal Fauzi Nurdianto Rosalinda Avia Eryatma Rury Eryna Putri Rusyad Adi Suriyanto Rusyad Adi Suryanto RWulandari, Septiayu Saamia, Vira Saleh, Tania Ardiani Setiawati, Rosy Simon Martin Manyanza Nzilibili Sispitasri, Yeti Eka Sri Puji Astuti W Sugiharto, Ade Firmansyah Sulistyorini, Nilly Suryanegara, I Ketut Heru Syarif, Ichsan Tambunan, Edwin M.B. Tiara Mayang Pratiwi Lio Titik Erliyah Toetik Koesbardiati Utomo, Ratno Tri Wahyu Widodo Wahyuningsih, Astuti Wimbuh Tri Widodo Wresti Indriatmi B. Makes wulandari, septiayu Yessy Andriani Fauziah Yeti Eka Sispita Sari Yeti Eka Sispitasri