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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Majalah Kedokteran Bandung Medical Journal of Indonesia Folia Medica Indonesiana Jurnal Biosains Pascasarjana JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) MPI (Media Pharmaceutica Indonesiana) Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Teknologi Laboratorium Berkala Arkeologi SANGKHAKALA Journal Of Vocational Health Studies Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Berkala Arkeologi Indonesian Journal of Chemistry Meditory : The Journal of Medical Laboratory Poltekita : Jurnal Ilmu Kesehatan Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Ilmu Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Cendekia : Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology International Islamic Medical Journal Jurnal Hukum Kesehatan Indonesia Scolae: Journal of Pedagogy Innovative: Journal Of Social Science Research Ranah Research : Journal of Multidisciplinary Research and Development Sriwijaya Journal of Forensic and Medicolegal Jurnal Forensik dan Medikolegal Indonesia Berkala Arkeologi Tasyri' : Jurnal Muamalah dan Ekonomi Syariah Prosiding International Conference on Sustainable Innovation (ICoSI) Jurnal of Syiah Kuala Dentistry Society
Ahmad Yudianto
Department Of Forensic And Medicolegal Medicine, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Transportation Veterinary Other

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DNA TOUCH STR CODIS AS LEGITIMATE EVIDENCE IN UNCOVERING CRIMINAL ACTS Nurdianto, Arif Rahman; Setiawan, Fery; Yudianto, Ahmad; Nurdianto, Rizal Fauzi; Sunariani, Jenny
Jurnal Biosains Pascasarjana Vol. 26 No. 1 (2024): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v26i1.2024.77-86

Abstract

The identification process at the crime scene (TKP) is one of the essential tasks carried out by police investigators. One critical aspect is the collection and analysis of evidence found at the crime scene, referred to as biological evidence (BB). This evidence serves as a crucial source for determining whether an incident occurred and, if so, whether it was a suicide or another criminal act (such as murder, etc.). The biological evidence found can be analyzed using molecular serobiology approaches, which are rapidly advancing in the fields of forensic medicine and medicolegal practice. One such approach involves utilizing DNA traces, although a common challenge is that often the DNA recovered at crime scenes is degraded. Therefore, a DNA amplification method utilizing the Polymerase Chain Reaction (PCR) technique, along with the short tandem repeat (STR) – CODIS identification approach, is necessary. The analysis of DNA traces using the STR-CODIS approach enables investigators to determine whether the incident was purely criminal, a suicide, or another type of accident involving the victim. Furthermore, the DNA analysis using the STR-CODIS method can also aid in identifying the victims discovered at the crime scene.
PENERAPAN MODEL PEMBELAJARAN ACTIVE LEARNING DALAM MENINGKATKAN HASIL BELAJAR PESERTA DIDIK PADA MATA PELAJARAN AQIDAH AKHLAK DI MTs. ALKHAIRAAT MADINATUL ILMI DOLO Yudianto, Ahmad
Scolae: Journal of Pedagogy Vol 6 No 1 (2023): Volume 6 (1) Juni 2023
Publisher : Sekolah Tinggi Keguruan dan Ilmu Pendidikan (STKIP) Dampal Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56488/scolae.v6i1.116

Abstract

This research discusses the implementation of the Active Learning model in improving students' learningoutcomes in the subject of Islamic faith and morals at MTs Alkhairaat Madinatul Ilmi, Dolo District, Sigi Regency. Themain issues addressed in this thesis are: How is the implementation of the Active Learning model in the subject ofIslamic faith and morals? What steps are used in implementing the Active Learning model in the subject of Islamic faithand morals? What are the obstacles and solutions to the Active Learning model in the subject of Islamic faith andmorals?The research approach is qualitative, employing data collection techniques such as observation, interviews,documentation, followed by data reduction, data presentation, and verification through triangulation methods.The results of this thesis research indicate that the implementation of the Active Learning model greatlyassists educators and students in the teaching and learning process. Students are encouraged to create an atmospherewhere they actively ask questions, inquire, and develop ideas, thereby becoming the center of learning activities.Educators act as facilitators, motivators, and inspirators, controlling, supervising, and providing opportunities forstudents to think critically, develop inspiration, and creativity. The implementation involves designing learningactivities, motivating students, organizing them, and presenting the material. Challenges in implementing the ActiveLearning model include students' lack of confidence and limited time allocation, while solutions involve motivatingstudents and training them to articulate thoughts and manage time effectively.
IDENTIFICATION OF HUMAN HAIR USING DIRECT PCR TARGETING THE CYTOCHROME B GENE Tri Widodo, Wimbuh; Masjkur, Indah Nuraini; Huda, Qurrota A’yunil; Yudianto, Ahmad; Kristianto, Sonny; Putri, Rury Erina
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3784

Abstract

Background: Hair is one of the human parts that is easily scattered and easy to obtain for testing. Direct PCR which is PCR without going through the DNA extraction process has been widely used in various fields. The cytochrome b gene is one of the genes located in mitochondrial DNA. The gene is unique in that it has a small variation within one species organism but has a large variation between species. Aims: c. Methods: Hair was heated at 600C for 10 minutes then the sample was used for the PCR template. PCR was performed using the cytochrome b gene. The results: The analysis showed that human hair could still be detected on day 30 using direct PCR using the cytochrome b gene. Conclusions: This result will simplify and save time in analysis in various fields related to hair shafts.
PEMERIKSAAN TOKSIKOLOGI FORENSIK DALAM KASUS KERACUNAN GAS HIDROGEN SULFIDA ; STUDI PUSTAKA Tambunan, Edwin; Yudianto, Ahmad
Jurnal Forensik dan Medikolegal Indonesia Vol 3 No 1 (2021): Jurnal Forensik dan Medikolegal Indonesia
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Toksikologi merupakan cabang ilmu yang mempelajari tentang racun, yang semakin maju seiring dengan perkembangan ilmu pengetahuan dan teknologi. Kasus keracunan memang jarang terjadi, namun perlu mendapat perhatian khususnya oleh dokter forensik. Keracunan yang jarang terjadi dalam praktek forensik diantaranya adalah keracunan gas hidrogen sulfida (H2S). H2S adalah gas yang tidak berwarna, bau menyengat seperti telur busuk, mudah terbakar dan bersifat eksplosif. H2S banyak ditemukan di peternakan dan pabrik. Pernah dilaporkan peningkatan kasus bunuh diri menggunakan gas H2S terjadi di Amerika Serikat. Pada tingkat rendah gas H2S hanya mengiritasi konjuntiva, sklera dan saluran nafas bagian atas, sedangkan pada tingkat yang lebih tinggi (1000-2000 ppm) dapat menyebabkan kematian yang cepat. Studi tinjauan pustaka ini menunjukkan temuan yang tidak spesifik dari otopsi kasus keracunan H2S, diantaranya perubahan warna menjadi kehijauan pada gray matter otak. Dari hasil uji toksikologi pada keracunan gas H2S, didapatkan kadar tiosulfat yang tinggi dalam darah.
Characterization of DNA Quality, TPOX Locus, TH01 Locus in Seawater Soaked Nails in Forensic Identification Utomo, Ratno Tri; Endarini, Lully Hanni; Yudianto, Ahmad; Christyaningsih, Juliana
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 3 (2023): November
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i3.3028

Abstract

Forensic science is multiplying. One of them is the identification of events at sea. Of course, the victim’s body can be submerged in seawater for days so that components in seawater can affect the victim’s body. This research aimed to see the effect of storage time on the level and purity of DNA in nail samples. Identification of nail DNA using the Polymerase Chain Reaction (PCR) method. The DNA purity test examines nail samples using a spectrophotometer read at 260 and 280 nm wavelengths. Measurement of DNA levels using the PCR instrument on the STR TPOX locus, the result was 32.02 ug/uL on positive control, 36.73 ug/uL in seawater immersion on the 2nd day, 35.35 ug/uL in seawater immersion on the 7th day, and 34.5 ug/uL in seawater immersion on the 20th day. At the STR TH01 locus, the result was 32.155 ug/uL on positive control, 38.05 ug/uL on the 2nd day of immersion in seawater, 35.18 ug/uL on the 7th day, and 33.88 ug/uL on the 20th day. Results Examination of the Total Plate Number of seawater on the 2nd day, 7th day, and 20th day of immersion found no colonies of germ colony growth. This study obtained sufficient DNA purity from the 2nd day, 7th day and 20th day. So that the samples could be analyzed for DNA levels, the result obtained from the study showed a decrease in DNA levels at the TPOX Locus and TH01 Locus from the 2nd day, 7th day, and 20th day.
Revitalisasi Pendidikan Karakter Berbasis Pancasila Dalam Pembinaan Remaja Putus Sekolah Meli, Meli; Nurdin, Erni; Yudianto, Ahmad
Innovative: Journal Of Social Science Research Vol. 5 No. 3 (2025): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v5i3.19883

Abstract

This study aims to examine the revitalization of Pancasila-based character education in the development of dropout adolescents at the Palu Children's Social Institution UPT. Dropout adolescents face the risk of identity crisis, behavioral deviation, and weak value orientation. Character education is an important strategy to form an independent and moral person. The approach of this research is qualitative descriptive with a case study strategy. Data collection techniques include in-depth interviews, participatory observation, and documentation. The results of the study indicate that character education is implemented through habituation and role models, with the integration of Pancasila values ​​in development activities, such as worship, group discussions, social cooperation, and division of tasks. Supporting factors include the commitment of the mentor, cross-institutional cooperation, and the religious environment of the institution. The obstacles include the social background of adolescents, limited facilities, and lack of mentor training. Pancasila-based character education that is implemented contextually and consistently can strengthen the moral and social identity of dropout adolescents.
VARIASI GENETIK LOCI STR CODIS (THO1,TPOX) MANUSIA GILIMANUK (PULAU BALI) Koesbardiati, Toetik; Yudianto, Ahmad; Murti, Delta Bayu; Suriyanto, Rusyad Adi
Berkala Arkeologi Vol. 33 No. 2 (2013)
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30883/jba.v33i2.11

Abstract

It is assumed that Mongoloid’s migration came from western and northern part of Indonesia in various waves of migration. The migrant population then mixed with initial inhabitants, which are Australomelanesoid. The wave of migration moved further to the eastern Indonesia and mixed with migrant that entered from east (Papua). Some researches show that the concentration of mixture (hybridization) of migration was around Wallace’s line. Gilimanuk is one of prehistoric site that yields Neolithic human remains. It is assumed that Gilimanuk can give worthy information about human variation at that time. The aim of the research is to describe the human genetic variation at site of Gilimanuk. The material is DNA (deoxyribonucleic acid) has been extracted from many piece of bone of Gilimanuk’s human remains. We used STR (short tandem repeat) two loci (THO1 and TPOX) to gain human genetic variation. The result show all of sample yields band with different allele. This evidence confirms that they have a genetic affinity is not the same, or their genes from several population.
DNA quality and quantity in adipose tissue: a comparison of the effects of bomb explosion Leonardo; Sugiharto, Ade Firmansyah; Indriatmi, Wresti; Atmadja, Djaja Surya; Yudianto, Ahmad; Herkutanto; Widodo, Wahyu
Medical Journal of Indonesia Vol. 32 No. 4 (2023): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247206

Abstract

BACKGROUND Adipose tissue is often overlooked in DNA testing due to misconceptions about its DNA content. However, its shock-absorbing qualities may be useful for high-pressure scenarios like bomb blasts. This study aimed to evaluate DNA quality and quantity in adipose tissue affected by blasts compared to that in unaffected tissue. METHODS 10 adipose tissue samples were taken from regions near and far from the blast, representing the blast-exposed and non-blast-exposed groups. The adipose tissue was stored at a low temperature for 5 days, after which an organic extraction method was applied. The purity of the DNA extract was assessed using a NanoDrop spectrophotometer, and its integrity was evaluated using 0.8% concentration gel electrophoresis at 60 V for 90 min. DNA typing was conducted using the GlobalFiler™ kit, and DNA quantity was determined with the Quantifiler™ Trio DNA Quantification kit. RESULTS Of 20 DNA extracts from adipose tissue, all samples demonstrated purity, integrity, and complete typing results. Adequate integrity was found in 90% of samples in both groups. A 50% incidence of allele shifting was observed at the D7S820 locus within the blast-exposed group. CONCLUSIONS DNA from blast-exposed adipose tissue exhibited no significant quality or quantity differences from non-blast-exposed tissue. This suggested adipose tissue’s potential as an alternative DNA source in a bomb explosion.
Bioactivity of Sengkubak (Pycnarrhena cauliflora Diels.) Leaf Ethanolic Extract as Antioxidant and Antibacterial Streptococcus Sanguinis Cicilia, Cindy Oktavi; Yudianto, Ahmad; Putri, Rury Eryna
Ranah Research : Journal of Multidisciplinary Research and Development Vol. 8 No. 1 (2025): Ranah Research : Journal Of Multidisciplinary Research and Development
Publisher : Dinasti Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/rrj.v8i1.1922

Abstract

Pycnarrhena cauliflora Diels. known as Sengkubak, is a natural-based potential antioxidant and antibacterial from the Menispermaceae family. Streptococcus sanguinis is a primary colonizer bacteria that is consistent in biofilm formation. This study attempted to identify S. sanguinis inhibition from antibacterial and antioxidant bioactivity of sengkubak leaf extract. Sengkubak leaves samples were extracted with 70% ethanol through 72 hours (3x24) maceration. Phytochemicals were identified qualitatively and quantitatively through analysis of total quercetin and phenolic content. Antioxidants were tested using the DPPH method. S. sanguinis sensitivity was tested through liquid microdilution method. The extract of sengkubak leaves has a yield of 16.30% (w/w). Qualitative screening showed positive results for flavonoids, phenols, alkaloids, saponins, terpenoids, tannins, and steroids. TFC (quercetin) in extract was 5.22±0.969 QE/g and TPC was 4.08±1.005 mg GAE/g. The IC50 antioxidant value of extract was 40.136 µg/mL. The minimum inhibitory concentration value is shown at 5×10⁴ µg/mL. This research study proves that ethanol extract of sengkubak leaves holds various phytochemical compounds which are likely utilized as potent antioxidants and have inhibitory effect against S.sanguinis.
Co-Authors A’yun, Qurrota Achmad Basori, Achmad Acrivida Mega Charisma Agung Sosiawan Amalia Rozaiza Ightikhoma Anak Agung Putu Santiasa Putra Andika Aliviameita Apriliani, Herlina Arfianti, Evy Arif Rahman Nurdianto Arif Rahman Nurdianto Ariyanto Wibowo Ariyanto Wibowo Ariyanto Wibowo, Ariyanto Aung, Htet Htet Azizatul Haq Larasati Biqisthi Ari Putra Cicilia, Cindy Oktavi Deka Bagus Binarsa Delita Bayu Murti Delta Bayu Murti Desy Martha Panjaitan Djaja Surya Atmadja Endarini, Lully Hanni Evan Boedi Dewanto Eveline Yulia Darmadi Fery Setiawan Fery Setiawan Furqoni, Abdul Hadi Heni Puspitasari Heribertus Agustinus B Tena Herkutanto Huda, Qurrota A’yunil Hutagalung, Magda Rosalina Ida Bagus Narmada Imam Susilo Indah Nuraini Indah Nuraini Indah Nuraini Indah Nuraini Masjkur Indah Nuraini Maskjur Indah Nuraini, Indah Januar Alif Jenny Sunariani Jenny Sunariani Jenny Sunariani, Jenny Juliana Christyaningsih Kristianto, Sonny Latief Mooduto, Latief Leonardo Margaret, Nola Masniari Novita Meli Meli, Meli Mely Purnadianti Mieke Sylvia Mochammad Yuwono Muhammad Afiful Jauhani Muhammad Kholil Ikhsan Muktiningsih Nurjayadi Nazaratun Thaiyibah Nazaratun Thaiyibah, Nazaratun Nily Sulistyorini Nola Margaret Nola Margaret NOVITASARI Novitasari Novitasari Nurdianto, Arif Rahman Nurdin, Erni Perwira, Satria Prabowo, Yudha Erik Prasilia Ramadhani Puspa Wardhani Puspa Wardhani Puspa Wardhani Putra, Bilqisthi Ari Putri, Dwi Fitrianti Arieza Putri, Rury Erina Qurrota A'yunil Huda Rahma Diyan Martha Reni Sumino Rizal Fauzi Nurdianto Rosalinda Avia Eryatma Rury Eryna Putri Rusyad Adi Suriyanto Rusyad Adi Suryanto RWulandari, Septiayu Saamia, Vira Saleh, Tania Ardiani Setiawati, Rosy Simon Martin Manyanza Nzilibili Sispitasri, Yeti Eka Sri Puji Astuti W Sugiharto, Ade Firmansyah Sulistyorini, Nilly Suryanegara, I Ketut Heru Syarif, Ichsan Tambunan, Edwin M.B. Tiara Mayang Pratiwi Lio Titik Erliyah Toetik Koesbardiati Utomo, Ratno Tri Wahyu Widodo Wimbuh Tri Widodo Wresti Indriatmi B. Makes wulandari, septiayu Yessy Andriani Fauziah Yeti Eka Sispita Sari Yeti Eka Sispitasri