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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Majalah Kedokteran Bandung Medical Journal of Indonesia Folia Medica Indonesiana Jurnal Biosains Pascasarjana JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) MPI (Media Pharmaceutica Indonesiana) Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Teknologi Laboratorium Berkala Arkeologi SANGKHAKALA Journal Of Vocational Health Studies Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Berkala Arkeologi Indonesian Journal of Chemistry Meditory : The Journal of Medical Laboratory Poltekita : Jurnal Ilmu Kesehatan Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Ilmu Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Cendekia : Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology International Islamic Medical Journal Jurnal Hukum Kesehatan Indonesia Scolae: Journal of Pedagogy Innovative: Journal Of Social Science Research Ranah Research : Journal of Multidisciplinary Research and Development Sriwijaya Journal of Forensic and Medicolegal Jurnal Forensik dan Medikolegal Indonesia Berkala Arkeologi Tasyri' : Jurnal Muamalah dan Ekonomi Syariah Prosiding International Conference on Sustainable Innovation (ICoSI) Jurnal of Syiah Kuala Dentistry Society
Ahmad Yudianto
Department Of Forensic And Medicolegal Medicine, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Transportation Veterinary Other

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Clinical Forensic Assessment of Victims with Stab Wound Prabowo, Yudha Erik; Suryanegara, I Ketut Heru; Perwira, Satria; Yudianto, Ahmad
Jurnal Medis Islam Internasional Vol 6 No 1 (2024): June
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/iimj.v6i1.5898

Abstract

Introduction: Violent crimes using sharp objects are a common problem in Indonesia because sharp weapons can be easily accessed. When evaluating sharp-force injuries, the correct interpretation of the morphology of individual wounds, as well as the overall pattern of findings, is of paramount importance in the reconstruction aspect of the crime. Any knowledge of the occurrence and distribution of sharp force injuries in knife attack victims and perpetrators can help to verify statements or expose defensive lies. Case: We present, a patient from another hospital came to the emergency room at Doctor Soetomo Hospital with a stab wound to the right chest. The 35-year-old male victim, with the initials SPJ, admitted that he was stabbed by his brother. The victim's vital signs were stable when examined. On the right chest, an open wound with flat edges and sharp and blunt angles was found, measuring three centimeters long and five centimeters wide. The victim underwent radiological and laboratory examinations, installed water-sealed drainage, and was administered anti-pain and antibiotics.  Discussion: The qualification for this patient's wound based on the Indonesian Criminal Code and the scoring carried out is a moderate wound Conclusions: Based on the law and the scoring carried out by the author, the wounds on the patient's body are injuries resulting from a criminal act and are included in the category of moderate abuse, which means that the injuries that occurred to the victim did not cause death or cause permanent disability but caused temporary obstacles in carrying out work.
VALIDATION OF HEADSPACE SOLID-PHASE MICROEXTRACTION WITH GAS CHROMATOGRAPHY-FLAME IONISATION DETECTOR METHOD FOR ALCOHOL ANALYSIS ON GASTRIC FLUID Thaiyibah, Nazaratun; Yuwono, Mochammad; Yudianto, Ahmad
Jurnal Kimia Riset Vol. 8 No. 2 (2023): December
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v8i2.44502

Abstract

Identifying the concentration of alcohol compounds in postmortem analysis of biological fluids can help the investigation, just as postmortem analysis of gastric juices can reveal the concentrations of alcohol consumed. However, an efficient and effective combination of extraction and measurement methods is required when looking at complex postmortem samples. Therefore, a headspace-solid phase microextraction (HS-SPME) method was developed using gas chromatography with a flame ionization detector (GC-FID) to identify postmortem alcohol concentrations. This present study optimizes and validates an effective and efficient method for postmortem alcohol extraction and quantification in gastric fluid. The optimal conditions for HS-SPME extraction using 65μm Polydimethy lsiloxane Divinyl benzene (PDMS/DVB) fiber for analyte isolation were 15 minutes. at 60°C and an exposure time of 1 minute. The validation investigation shows that the suggested approach satisfies the criteria for linearity, precision, accuracy, LOD, and LOQ for postmortem measurement of alcohol in gastric fluid.
Trauma Profile of Homicide Victims at Dr. Soetomo General Academic Hospital, Surabaya, during the COVID-19 Pandemic Syarif, Ichsan; Yudianto, Ahmad; Saleh, Tania Ardiani; Sulistyorini, Nily
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 15 No. 1 (2024): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V15I12024.17-22

Abstract

Highlights: Most homicide victims were predominantly in the productive age range of 21-40 years old. Males were more likely to be homicide victims than females.   Abstract Introduction: The injuries of homicide victims are clues in forensic medicine to uncover a possible crime. In Indonesia, there are not many studies on trauma profiles, specifically in homicide cases. This study aimed to provide an overview of the trauma profile of homicide victims at Dr. Soetomo General Academic Hospital, Surabaya, from January 2020 until December 2021. Methods: This was a descriptive study. Secondary data was collected from 34 forensic examination reports at Dr. Soetomo General Academic Hospital, Surabaya. Data such as age, sex, type of injury, sites of injury, number of injuries, cause of death, and mechanism of death were analyzed statistically. Results: Most victims were between 21 and 30 years old and 31 and 40 years old (26.47%). Sex was dominated by men (76.47%). The most common type of injury was abrasion (25.71%). The most common injury sites were head-neck (34.34%) and chest-upper back (34.34%). The most common number of injuries was ≥10 (44.12%). The most common cause of death was sharp-force trauma (54.54%), and the most common mechanism of death was exsanguination (54.54%). Conclusion: Males predominated in the age ranges of 21 to 30 years old and 31 to 40 years old. The most common type of injury was abrasion. The most common sites of injury were the head, neck, and chest upper back. The most common number of injuries was ≥10. Most victims died of sharp-force trauma. Exsanguination was the most common mechanism of death.
Limit Detection of Short Tandem Repeats (STR) Analysis on Touch DNA Samples Saamia, Vira; Yudianto, Ahmad; Nurjayadi, Muktiningsih; Novitasari, Novitasari; Furqoni, Abdul Hadi
Indonesian Journal of Chemistry Vol 24, No 5 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.94081

Abstract

Forensic short tandem repeats (STR) profiling on touch DNA samples has emerged as a primary method for human identification. The stability and uniqueness of STR combination from the targeted locus in each individual make it a precision marker for human identification. Touch DNA samples can be found in traces of biological material shed from a person. This work aimed to identify the lowest concentration limit required for generating an interpretable DNA profile and the sensitivity of the STR loci applied. Touch DNA samples were collected from donors who were asked to hold a rope for 5 min. A double swab technique was used to lift the touch samples from the rope. These samples are subjected to DNA extraction and quantification. Two STR amplification cycles, 29 and 34 cycles, were used. DNA concentration greatly influences the success of amplifying the target allele at each STR locus to be interpreted into a complete DNA profile, shown by its allele peak. Touch DNA concentration >0.25 ng can produce a complete DNA profile. LCN method successfully amplified touch DNA with a concentration 0.0625–0.25 ng/µL. Limit detection of touch DNA analysis is 0.25 ng/µL. Low-copy DNA can still be analyzed within 0.0625–0.25 ng/µL.
EFFECT OF POST MORTEM INTERVAL TOWARD Î’-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS Susilo, Imam; Putra, Bilqisthi Ari; Yudianto, Ahmad
Journal of Vocational Health Studies Vol. 7 No. 2 (2023): November 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V7.I2.2023.107-114

Abstract

Background: The effects of post mortem interval on tryptase levels are unclear and have been challenging to quantify due to limited and at times contradictory research. Purpose: Analyze the effects of Post Mortem Interval (PMI) due to anaphylactic shock on the expression of β-tryptase and mast cell chymase in the heart organ. Method: This type of research was an experimental study with a randomized block design (RBD) with the same subjects and a time series for 24 hours, using 5 (five) rabbits. The measurement of β-tryptase and chymase expression was based on immunohistochemical IRS (immunoreactive score) in the heart organs of rabbits experiencing anaphylactic shock sensitization and induction of anaphylactic shock using ovalbumin. Result: Based on the results of statistical tests using univariate analysis, there was a relationship between the length of death (post mortem interval) and the expression of mast cells β-Tryptase and cardiac chymase (p-value = 0.006) and chymase (p-value = 0.002) with (p-value <0.05). The effective test time with β-tryptase markers using cardiac organs was prone to long death time of 0 - 15 hours. The effective check-up time with chymase markers using the heart organ was at a long time of death of 9 hours. Conclusion: The results of the study showed that tryptase and chymase could be used as markers of anaphylactic shock.
Refleksi Kritis Hukum Pajak dalam Perspektif Ekonomi Syariah: Refleksi Kritis Hukum Pajak wulandari, septiayu; Ahmad Yudianto
Tasyri' : Jurnal Muamalah dan Ekonomi Syariah Vol. 5 No. 1 (2023): Jurnal Tasyri: Jurnal Muamalah dan Ekonomi Syariah
Publisher : Fakultas Hukum dan Ekonomi Syariah Institut Agama Islam Pangeran Dharma Kusuma Indramayu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55656/tjmes.v5i1.216

Abstract

A critical reflection on tax law from the perspective of Islamic economics is crucial in the context of sustainable economic development based on Islamic principles. In this abstract, we analyze the challenges and opportunities in integrating Islamic economic principles into the tax system, and its implications for justice, efficiency, and economic sustainability. We highlight the need for in-depth studies on aspects of tax law that are inconsistent with Islamic economic principles, and efforts to develop alternatives that align with Islamic values. Based on this analysis, we conclude that critical reflection on tax law from the perspective of Islamic economics can significantly contribute to creating a fairer, more efficient, and sustainable tax system.
Touch DNA viability on various substrates from different shedder levels Saamia, Vira; Yudianto, Ahmad; Nurjayadi, Muktiningsih; Novitasari
Medical Journal of Indonesia Vol. 33 No. 3 (2024): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247398

Abstract

BACKGROUND Touch DNA samples are frequently discovered at crime scenes, including those found at the scene, on the victim, with the suspect, or on objects related to the incident. This study aimed to investigate 3 key factors affecting touch DNA samples: the characteristics person that shed the DNA, surfaces variants where the DNA was deposited, and different sampling methods effectiveness that influence DNA quantity, quality, and detection. METHODS 9 participants grouped into high, intermediate, and low shedder levels simultaneously tied 2 types of ropes, non-porous and porous. The first person will hold a rope for 5 min then pass it to the second person to hold on the same spot for another 5 min. DNA was collected from each rope using the double swab and tape-lift method, extracted, and quantified using real-time polymerase chain reaction (PCR). Touch DNA profile at 20 short tandem repeat loci was amplified in PCR system and detected on capillary electrophoresis. RESULTS Type of substrate (p = 0.97) or sampling method (p = 0.053) used for touch DNA collection did not significantly impact the DNA yield or profiling outcomes. A notable difference (p<0.001) was found in DNA quantity between high, intermediate, and low shedders, regardless of the substrate or method used. CONCLUSIONS Individual shedder level has a greater influence on the results of touch DNA analysis regarding the DNA quantity and profiling quality than substrate type and sample procedure.
CODIS STR LOCI (CSF1PO, THOI, TPOX, vWA) GENETIC VARIATION ANALYSIS IN MADURESE Yudianto, Ahmad; Sosiawan, Agung; Margaret, Nola
Folia Medica Indonesiana Vol. 52 No. 1 (2016): JANUARY - MARCH 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.763 KB) | DOI: 10.20473/fmi.v52i1.5196

Abstract

Endogamy continues to occur among the Madurese people in rural areas of the island of Madura, especially those areas of the smallest islands around the mainland of Madura. Endogamy as seen from a genetic standpoint will increase the frequency of homozygote genotypes. With regard to genetic variations, STRs of nuclear DNA and polymorphisms in mtDNA are frequently examined. Genetic variations in human undergo an evolutionary process through the accumulation of changes in DNA sequence, i.e. through the process of nucleotide substitutions that evolves in number with the directional development of lineage. So far, the genetic variations among the populations in Madura Island have not been known. The present study was an observational analytical research with the purpose of determining the genetic variations in STR CODIS in the populations of Madura Island. Results indicated that, based on loci alelle: CSF1PO, THOI, TPOX, and vWA, there was homozygote genotypes. The allele variations is not specific for Madurese ethnic but this variations may represent married model in Madurese ethnic. According to Mustama (2007), a gene pool is not only a collection of genes but a dynamic system organized and containing the past history of a population. Any genetic information has certain historical, anthropological and statistical aspects necessitating an interdisciplinary coordination and collaboration.
NUCLEOTIDE VARIANCE OF MITOCHONDRIAL DNA D-Loop 126 bp (nt: 34-159) REGION IN MADURESE Sulistyorini, Nilly; Yudianto, Ahmad; Margaret, Nola
Folia Medica Indonesiana Vol. 52 No. 2 (2016): APRIL - JUNE 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.976 KB) | DOI: 10.20473/fmi.v52i2.5215

Abstract

Endogamy continues to occur among the Madurese people in rural areas of the island of Madura, especially those areas of the smallest islands around the mainland of Madura. Endogamy as seen from a genetic standpoint will increase the frequency of homozygous genotypes. With regard to genetic variations, STRs of nuclear DNA and polymorphisms in mtDNA are frequently examined. Mitochondrial variations in the human undergo an evolutionary process through the accumulation of changes in DNA sequence, i.e. through the process of nucleotide substitutions that evolves in number with the directional development of lineage. So far, the genetic variations among the populations in Madura Island have not been known. The present study was an observational analytical research with the purpose of determining the genetic variations in the polymorphisms of 126-bp mtDNA D-Loop HV2 (nt: 34-159) in the populations of Madura Island. Results indicated that, based on the homology analysis with rCRS sequence, there were 9 variants consisting of two transition mutations, 6 transversion mutations, and one insertion mutation. This indicates that a transversion mutation had a higher probability than transition and insertion mutations. According to Mustama (2007), a gene pool is not only a collection of genes but a dynamic system organized and containing the past history of a population. Any genetic information has certain historical, anthropological and statistical aspects necessitating an interdisciplinary coordination and collaboration.
ANALYSIS OF EARPHONE SWAB MITOCHONDRIAL DNA AS AN ALTERNATIVE MATERIAL FOR IDENTIFICATION EXAMINATION Yudianto, Ahmad; Sispitasri, Yeti Eka; Margaret, Nola
Folia Medica Indonesiana Vol. 52 No. 3 (2016): JULY - SEPTEMBER 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.34 KB) | DOI: 10.20473/fmi.v52i3.5446

Abstract

Identification include fingerprint, property, medical, dental, serologic and exclusion methods. In the development, identification methods led to molecular forensics, a new field of science evolving since the 1980s, known as DNA fingerprinting. Specimens widely used in DNA assay for identification are blood spots/bloods, semen spots, vaginal swabs, buccal swabs and bones. In addition to these specimens, the last objects often used by the perpetrators/victims can be used, such as hearing aids (headsets/earphones). In its use, earphones are attached to the outer ear skin; thus, the earwax is suspected to adhere to the device. To date, in Indonesia personal identification is performed through swabs of earphones/headsets using the DNA profiling method. In particular, mitochondrial DNA has not been widely used for identification. The present study was of laboratory experimental. Earphones which have been used for 3 days were placed in room temperature for 1, 7, 14 and 20 days. Results showed that the environmental factor of exposure duration had an effect of a significant decrease in the levels of DNA from day 1 to day 20. Only 126-bp mtDNA (HVS II) was detected on the samples of day 1 and continued with sequencing. Mitochondrial DNA has better durability and relatively higher number of copies than those of nuclear DNA. This leads to greater possibility of success in amplification, given the higher number of mitochondrial DNA copies and the fact that mitochondrial DNA is a single locus that allows recombination.
Co-Authors A’yun, Qurrota Achmad Basori, Achmad Acrivida Mega Charisma Agung Sosiawan Amalia Rozaiza Ightikhoma Anak Agung Putu Santiasa Putra Andika Aliviameita Apriliani, Herlina Arfianti, Evy Arif Rahman Nurdianto Arif Rahman Nurdianto Ariyanto Wibowo Ariyanto Wibowo Ariyanto Wibowo, Ariyanto Aung, Htet Htet Azizatul Haq Larasati Biqisthi Ari Putra Cicilia, Cindy Oktavi Deka Bagus Binarsa Delita Bayu Murti Delta Bayu Murti Desy Martha Panjaitan Djaja Surya Atmadja Endarini, Lully Hanni Evan Boedi Dewanto Eveline Yulia Darmadi Fery Setiawan Fery Setiawan Furqoni, Abdul Hadi Heni Puspitasari Heribertus Agustinus B Tena Herkutanto Huda, Qurrota A’yunil Hutagalung, Magda Rosalina Ida Bagus Narmada Imam Susilo Indah Nuraini Indah Nuraini Indah Nuraini Indah Nuraini Masjkur Indah Nuraini Maskjur Indah Nuraini, Indah Januar Alif Jenny Sunariani Jenny Sunariani Jenny Sunariani, Jenny Juliana Christyaningsih Kristianto, Sonny Latief Mooduto, Latief Leonardo Margaret, Nola Masniari Novita Meli Meli, Meli Mely Purnadianti Mieke Sylvia Mochammad Yuwono Muhammad Afiful Jauhani Muhammad Kholil Ikhsan Muktiningsih Nurjayadi Nazaratun Thaiyibah Nazaratun Thaiyibah, Nazaratun Nily Sulistyorini Nola Margaret Nola Margaret NOVITASARI Novitasari Novitasari Nurdianto, Arif Rahman Nurdin, Erni Perwira, Satria Prabowo, Yudha Erik Prasilia Ramadhani Puspa Wardhani Puspa Wardhani Puspa Wardhani Putra, Bilqisthi Ari Putri, Dwi Fitrianti Arieza Putri, Rury Erina Qurrota A'yunil Huda Rahma Diyan Martha Reni Sumino Rizal Fauzi Nurdianto Rosalinda Avia Eryatma Rury Eryna Putri Rusyad Adi Suriyanto Rusyad Adi Suryanto RWulandari, Septiayu Saamia, Vira Saleh, Tania Ardiani Setiawati, Rosy Simon Martin Manyanza Nzilibili Sispitasri, Yeti Eka Sri Puji Astuti W Sugiharto, Ade Firmansyah Sulistyorini, Nilly Suryanegara, I Ketut Heru Syarif, Ichsan Tambunan, Edwin M.B. Tiara Mayang Pratiwi Lio Titik Erliyah Toetik Koesbardiati Utomo, Ratno Tri Wahyu Widodo Wimbuh Tri Widodo Wresti Indriatmi B. Makes wulandari, septiayu Yessy Andriani Fauziah Yeti Eka Sispita Sari Yeti Eka Sispitasri