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All Journal HAYATI Journal of Biosciences El-Hayah : Jurnal Biologi Jurnal Bioterdidik: Wahana Ekspresi Ilmiah Bioedukasi: Jurnal Pendidikan Biologi QUANTUM: Jurnal Inovasi Pendidikan Sains Jurnal Penelitian Pertanian Terapan DIDAKTIKA BIOLOGI: Jurnal Penelitian Pendidikan Biologi Jurnal Bioedukatika BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Jurnal Lentera Pendidikan Pusat Penelitian LPPM UM Metro Abdi Masyarakat Penbios: Jurnal Pendidikan Biologi dan Sains At-Tajdid : Jurnal Pendidikan dan Pemikiran Islam Jurnal Pengabdian kepada Masyarakat Nusantara Mattawang: Jurnal Pengabdian Masyarakat BIOLOVA Sinar Sang Surya: Jurnal Pusat Pengabdian kepada Masyarakat EDUBIOLOCK Universitas Muhammadiyah Yogyakarta Undergraduate Conference Proceeding Madiun Spoor : Jurnal Pengabdian Masyarakat TRANSFORMASI : JURNAL PENGABDIAN PADA MASYARAKAT Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan Journal Of Human And Education (JAHE) Jurnal Humaniora dan Ilmu Pendidikan Makara Journal of Science Makara Journal of Technology POACE: Jurnal Program Studi Administrasi Pendidikan Jurnal Ilmiah Ilmu Terapan Universitas Jambi Open Community Service Journal PROSIDING SEMINAR NASIONAL PENELITIAN DAN PENGABDIAN KEPADA MASYARAKAT (SNPPM) UNIVERSITAS MUHAMMADIYAH METRO Jurnal Pengabdian Masyarakat dan Riset Pendidikan JISE
Agus Sutanto
Biologi Education Department, University Of Muhammadiyah Metro, Metro Lampung 34111, Indonesia
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Journal : HAYATI Journal of Biosciences

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In Vitro Medium-Term Storage of Banana Cultivar ‘Barangan’ Using Alginate-Encapsulation Technique Indrayanti, Reni; Wulandari, Nadia Fitria; Sutanto, Agus; Asharo, Rizal Koen; Adisyahputra
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.603-612

Abstract

In vitro medium-term storage using the alginate-encapsulation technique is essential for conserving plant genetic resources, preserving vegetatively propagated species by controlling the growth and development of explants, and maintaining plant biodiversity. The study aimed to obtain the optimum combination medium for the encapsulation of bananas and evaluate the viability of the alginate-encapsulated explants after storage. In vitro, medium- term storage of nodule-like meristem of banana cultivar ‘Barangan’ was performed using sodium alginate, paclobutrazol (PBZ), and Murashige and Skoog (MS) salt. This research consists of 3 stages: (1) Initiation and multiplication of in vitro shoots and nodule-like meristems; (2) In vitro medium-term storage of banana by encapsulation technique; (3) Regeneration of nodule-like meristem explants after in vitro storage. This research showed that the banana's number of nodule-like meristem was optimum on media supplemented with thidiazuron (TDZ) 0.22 mgL-1 and indole 3-acetic acid (IAA) 1.75 mgL-1. Encapsulation explant of nodules-like meristems using 3% sodium alginate in full and half-strength MS salt medium supplemented with PBZ 2.5 mgL-1 and incubated in liquid MS medium was able to store nodule-like meristem for six months. The color of the explants remains green, and the capsule is not damaged. Sub-culture of nodule-like meristem after storage in MS salt medium containing TDZ 0.22 mgL-1 and IAA 1.75 mgL-1 showed that nodules-like meristems could regenerate to form new shoots and nodule-like meristem three months after sub-cultured. This research concludes that encapsulated nodule-like meristem was recorded until six months of storage. PBZ was a necessary retardant in minimizing the growth during storage.
Co-Authors ., Achyani A.A. Ketut Agung Cahyawan W abrori, zaini Achyani Achyani Achyani, Achyani Ade Gunawan Adha Sabila Kifah Adisyahputra Afiatur Rohmah Agil Lepiyanto Agus Sujarwanta, Agus Ahmad Fatoni Akbar Bayhaqi, Muhammad Fiqih Alam, Tago Aminin, Sudirman Amiyati, Seprida Anak Agung Oka Andi Kurniawan Angelita, Gita Anggit Khendio Utomo Anjarwati, Sulis Aprilia, Anggi Arbasari, Maya Arifah Qurniani Arifin, Faisal Nur Arum Meidawati, Dita Asih Fitriana Dewi Asih Fitriana Dewi, Asih Fitriana Bagus Rachman Satrya Bagus Rachman Satrya Beny Saputra Budi Prasetyo cahyati, novia Cahyono, Heri Dacholfany, Ihsan Deni Kurnia Dio Anggoro Indarsyah, Gilang Dzaki Yaqiyuddin, Ahmad Eka Novita Sari Eka Reza Rifai Eka Setia Budi Eka Setyaningsih Elfrida Ratnawati Eppinga, Reinilde Fajri Arif Wibawa Fakhrurroja, Hanif Faza Rifàti, Eva Fenny Thresia Ferdiyansyah, Ilham Ferdy Sopran Wamala Gita Anggraeni Handoko Santoso Harjoko, Harjoko Harmoko Harmoko, Harmoko Hasminar Rachman Fidiastuti Helga, Anneta Hening Widiowati Hening Widowati Hifni Septina Carolina Hifni Septina Carolina, Hifni Septina Hikmah, Luluil Indriyani Putri Iswati Iswati Iswati Iswati Kartika Sari Kartiko, Yerry Kasmur, Roslinawati Khairunisa, Afiqah Khoirudin, M. Khoriudin, M Kusumasyari, Denti Laksono Trisnantoro Larasati, Anggia Dwi Leli Triana Lovely Son, Lovely M Rustam Rifai M. Ihsan Dacholfany Maharani, Adinda Purnama Mahfudin, Sidik mahmud, zenni Marzuki Noor, Marzuki Mellury, Deane Mia Cholvistaria Mia Cholvistaria, Mia Mufahroyin, Mufahroyin Muhammad Firdaus Muslihat, Muslihat Nani Septiana Nedi Hendri Nedi Hendri, Nedi Ningsih, Riyanti Nita, Wahyu Dian Novia Cahyati Nurhat Alamsyah Nurlaifa, Nadiah Putri Nyoto Suseno Oktavianti, Sinta Pipi Puspita Sari Prayitno, Dwi Purdiyanto, Aris Purwasih Purwasih, Purwasih Puspa, Elvina Maya Putri Aisyah, Putri Putri, Herlisa Hafizah Qurniani, Arifah Rahmawati, Nala Ramalida, Farida Rasuane Noor Rasuane Noor Reni Indrayanti Retnowati Retnowati Rifai, M Rustam - Rista, Paulina Eva Riswanto ., Riswanto riswanto riswanto Riyanto Riyanto Rizal Koen Asharo Rohimatusafiah, Yahya Rohmatul Anwar Rolia, Eva rosidha, nety RR. Ella Evrita Hestiandari Sahrin, Alfin SAPUTRA, BENY Sari, Novia Ambar Satriadi, Bekti Septiana, Nani Setyawan, Angga SHIHAB, M RIZKI Sholihah, Anisa Sinta Oktavianti Subardi, Subardi Bardi Sudaryanti Sudaryanti Sudirman AM Suharno Zen Sunardi Sunardi Suprayitno Suprayitno Supriyanto Supriyanto Susiani Susiani, Susiani Susiwati, Tri Syaferi, Akhmad Tasa Gandini Teguh Santoso Tika, Pujaan Tri Adi Wibowo, Tri Adi Triana Asih Triana Asih, Triana Triana, Leli Tut Wuri Handayani Tut Wuri Handayani, Tut Wuri Utami, Erna Utami, Intan Vironica, Elysa Wahidah, Nina Ikhwati wibowo, Vikto Agus Wicaksono Wibowo, Dimas Widowati, *Hening Widya Sartika Sulistiani, Widya Sartika Wimbawarni, Wiwik Wisca Febriana Wulandari, Nadia Fitria Yaya Rukayadi Yulistiana, Elza - Yuriana, Lely