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All Journal ACTA VETERINARIA INDONESIANA Jurnal Ilmiah Mahasiswa Veteriner Jurnal Pendidikan Dasar Albacore : Jurnal Penelitian Perikanan Laut Jurnal Medik Veteriner Jurnal Natural Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Jurnal Medika Veterinaria
Sari, Wahyu Eka
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Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Journal : Jurnal Natural

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Multi-drug resistance-1 (MDR-1) gene expression in MCF-7 cells after treated with doxorubicin-deoxyelephantopin combination and prediction of inhibitory activity against Pgp receptors with in silico DANIEL, DANIEL; FRENGKI, FRENGKI; JALALUDDIN, MUHAMMAD; SARI, WAHYU EKA; ROSMAIDAR, ROSMAIDAR; HASRIATI, HASRIATI; ZAKIAH, NONI
Jurnal Natural Volume 24 Number 3, October 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i3.33237

Abstract

Doxorubicin chemotherapy has been a strong focus in breast cancer research. Side effects, toxicity and resistance have been extensively studied. One proposed solution to these issues that its combination with deoxyelephantopin. Deoxyelephantopin is known to be toxic in many cancer cells but safe in normal cells. IC50 of each compound were determined by using a MTT assay, and the MDR-1 gene mRNA expression were determined by using qPCR method, while the interaction of doxorubicin in combination with deoxyelephantonin on Pgp receptor were predicted by using an in silico approach. The IC50 of deoxyelephantopin was found to be 11.2 g/mL, while IC50 of doxorubicin was 448 nM IC50 values showed a deoxyelephantopin-doxorubicin combination was able to reduce MDR-1 expression by 19% compared to doxorubicin and IC50 values indicated that the combination formula reduced the expression by 15% over doxorubicin alone. The affinity of doxorubicin and deoxyelephantopin is -12.16 kcal/mol and -9.51 kcal/mol, respectively, while the affinity of doxorubicin after combine with deoxyelephantopin decreases from -12,16 kcal/mol to -11,25 kcal/mol due to the release of one Leu 221 hydrogen bond. The combination of doxorubicin with deoxyelephantopin is able to reduce expression and suppress the function of the Pgp protein.
Co-Authors . Darniati Abrar, Mahdi Abrar Aftari, Nailul Akram, Saydul Amalia Sutriana Andi Novita Athaillah, Farida Awaluddin Awaluddin Azhari Azhari budianto panjaitan cut nila thasmi Daniel Daniel Darmawi Darmawi Daulay, Dina Khairani Erina Erina Erina Erina erma yusintha ramdhania Erwin Erwin Etriwati E Fadlah, Iga Faisal Jamin Ferdian, Riyan Frengki Frengki, Frengki Ginta Riady Hasanah, Lailatul Hasriati Hasriati, Hasriati Hasyimi, Adyar Hemelikova, Adela Henni Vanda Hennivanda Hennivanda Hilmi, Mulkiah Ismail Ismail ismail ismail ismail Jessica Anggun Safitri Kamaruzaman, Intan Noor Aina Khairunisa, Fauzia M Daud AK M Hasan M Nur Salim M. Hanafiah Maghfirah, Aura Mahdi Abrar Masda Admi Morizka Wianda Muhammad Hambal Muhammad Hambal Muhammad Hasan Muhammad Isa Muhammad Jalaluddin, Muhammad Musni, Nailah Muttaqien Muttaqien, Muttaqien Nanda Yulian Syah Noni Zakiah NURLIANA NURLIANA Nuzul Asmilia Panjaitan, Tasya Adilla Paula, Wita R. Rusli Rahmadhini, Vivi Rahmi Eka Putri Rahmi, Ulfa Rasmaidar Rasmaidar Rastina Rastina Razali Daud5 Riandi, Lian Varis Rizki, Maurina Rosa, Teuku Shaddiq rosalia rosalia Rosmaidar Rosmaidar, Rosmaidar Rumi Sahara Zamzami Sadri, Hidayatus Safika S, Safika Samsul Bahri Siti Aisyah Sugito - Sukardi Sukardi Syah, Nada Yulian T Reza Ferasyi Teuku Reza Ferasyi Teuku Zahrial Helmi Triva Murtina Lubis Triyuliani, Ririn Wardhani, Lailia Dwi Kusuma Waridah Waridah, Waridah Wilyo Hendro Fauzan Yona, Safetri Zakya Ardi Zamzami, Rumi Sahara Zulfayanto, Indri Zulkifli, Baidillah