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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF ENDOPHYTIC FUNGI EXTRACTS FROM THE FLOWER OF PURUN TIKUS (Eleocharis dulcis (Burm.f.) Trin. Ex Hensch.) Aini, Kurratul; Syarifah; Balkia, Siti Elisya; Febrianti, Sonia Wiji; Febrianti, Elizah
Berita Biologi Vol 25 No 1 (2026): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2026.13526

Abstract

Purun Tikus (Eleocharis dulcis (Burm.f.) Trin. Ex Hensch.) is a wetland plant native to Southeast Asia known to contain various bioactive secondary metabolites. However, studies on endophytic fungi associated with the flowers of this plant remain limited, even though endophytes are often capable of producing compounds similar to or even more potent than those of their host plants. This study aimed to identify and evaluate the antioxidant and antibacterial activities of endophytic fungi isolated from the flowers of E. dulcis. Isolation was carried out using surface sterilization and cultivation on Potato Dextrose Agar (PDA), followed by identification based on macroscopic and microscopic characteristics. Three isolates were successfully obtained: EDF1 (Neopestalotiopsis sp.), EDF2 (Lasiodiplodia theobromae), and EDF3 (Rhizopus sp.). Secondary metabolites were extracted using ethyl acetate, while antioxidant and antibacterial activities were evaluated using the DPPH method and agar disc diffusion method, respectively, against Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The results showed that each isolate exhibited different levels of bioactivity. Among them, EDF2 (L. theobromae) demonstrated the highest activity, with an IC₅₀ value of 19.149 µg/mL (categorized as very strong) and antibacterial inhibition exceeding 80% against all tested bacteria. These findings indicate that L. theobromae has strong potential as a natural source of bioactive compounds that can be further developed as candidates for phytopharmaceuticals, antimicrobial agents, and natural antioxidants through subsequent in vivo studies.
Development and Performance Evaluation of a Micro-Scale RDF Briquette–Fueled Steam Power Prototype Syarifah; Latricia Aina Hidayat; Rini Yunita Sari; Maratis, Jerry; Andri Krisna Hidayat; Pawenary; Hendri
Advance Sustainable Science Engineering and Technology Vol. 8 No. 2 (2026): February-April
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v8i2.3007

Abstract

Plastic waste is a major environmental problem in Indonesia due to its non-biodegradable nature. One innovative solution is converting waste into energy using Refuse-Derived Fuel (RDF) briquettes for small-scale power generation. This research designed and tested an RDF-based micro power plant prototype using briquettes composed of 80% dry organic biomass and 20% plastic for safe and stable combustion. The prototype consists of a combustion chamber, heat exchanger, impulse-type micro steam turbine driven by low-pressure steam, and a 12V low-speed DC generator. Performance was evaluated through temperature, voltage, current, power output, and efficiency measurements. The highest performance was achieved using 500 g of RDF, producing 0.02 A, 0.7 V, and 0.014 W at 135°C over 20 minutes, with an efficiency of 2.69×10⁻⁶%. Although efficiency was very low, the study demonstrates proof-of-concept feasibility and provides a baseline for future optimization of thermal and energy conversion efficiency.
Co-Authors A. Suharman Abdillah Afni Rosalina Al Ayyusuf, Muhammad Syufi Alam Bakti Almas Aqilah Amanda, Dhea Amin Nurokhman Andri Krisna Hidayat Aprilyani, Lutfia Arif Yachya Arif, Ali Arlina Nurbaity Lubis Asmar Balkia, Siti Elisya Bonaraja Purba Chairunny, Cici Claudia Marpaung DARMAN Defrin Deni Irawan, Deni Desinora Natalia Hutauruk Dhea Amanda Diancrisy Situmeang Dimas Ramdhan Dini Afriansyah Dwi Wahyuningsih Ebet Kogeya Een Mufaricha ENDRI ENDRI Erna Mutiara Eva Syahfitri Nasution, Eva Syahfitri Evawany Aritonang Fairuz Andalusia Faiza Muzaki Fariiz, Abdurrahman Fauzi, Andika Putra Pratama Febrianti, Elizah Febrianti, Sonia Wiji Firdaus, Aristophan Halinda Halinda Sari Lubis Hani, Kusumawardhani Fildzah Hariyadi, Ade Puji Herrian, Aulia Dwi Putri Hidayat Homsa, Anisa Nuraini Huaida, Siti Maria Ika Wulandari Ikhsan Darmawan ISMAIL HASAN Ismail Sukardi, Ismail Jefri kurniawan Jimmie Juanita4 Julio, Muhammad Jumiani Sarianti Kartika Keliat, Aisyah Br. Khairani Alawiyah Matondang Kurratul Aini, Kurratul laela Sari, laela Latricia Aina Hidayat Lilis Ernawati Lobo Sattu, Yuspin Lubis, Nasrullah Hidayat Lubis, Sri Novita M, Hasriani M. Zuhriadi Mahwiyah Maratis, Jerry Maria Innest Mayetti Mifta Ammaria Wulandari MUHAMMAD ALI Muhammad Bagas Ardhana Mutia Hafiza Nabila, Salsa Nazwah Fatimah Padli Padli Pawenary, Pawenary Pohan, Indah Zakia Pujiati Puput Maryati Purwandari, Vivi R Kintoko Rochadi R.Kintoko Rochadi Rahman, Imas Kania Ramadani Ramadani, Ayu Restu Febriyanti, Rachmanita Rini Yunita Sari Riri Novita Sunarti, Riri Rossa, Nia Rosulinawati Rudy Kurniawan Saragih, Gimelliya Sari, Disya Asjaumi Sari, Imas Hermala Seffina Irnanta Selfitrida A. Yani Sherina Silva Shodiq, Barli Silitonga, Nelson Siregar, Hairani Siska Afriany Siti Alifah Handayani Siti Maesaroh Siti Nurhidayah Al-Anshari Siti Zulaikha Soltan Takdir Sori Muda Sarumpaet Supriono, Slamet Syahrul Arya Ramadhan Syarifah Syarifnur Syarnubi, Syarnubi Tiara Posma Udur Hutapea Tukiman Turiyem Tutut Handayani Wahyuni Nurindah Sulistiowati Wahyuni, Arlinda Sari Wakerkwa, Ason Wiguna, Satria Yasmin, Noor Aini Yulia Tri Samiha Zaky Nuron Rizky Zuhriadi, M. Zulhaida Lubis Zulhijra, Zulhijra