Kadek Karang Agustina
Department Of Public Health, Faculty Of Veterinary Medicine, Udayana University, Denpasar, Indonesia

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Journal : Jurnal Medik Veteriner

Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Co-Authors Adhiputra, I Ketut Agus Indra Adiwinata, Putu Diva Ady Fendriyanto, Ady Ahmad Harits Fitra Mauladi Aida Lousie Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Dharmayudha Anak Agung Komang Suardana Andriawino Berdionis Sanam Anne Dawdoya Arinata, I Ketut Teguh Astrid Laboure Parahita Cesarina Pascalia dos Santos Alegria Daniella Everetta Desak Wiga Puspita Dewi Desy Ariani Ferdianti, Desy Ariani Dewa Akbar Maulana Dewa Made Dwi Parwata Dewi Titis Angganingrum Dimas Indra Dwi Purnama Dina Elviana DWI SURYANTO Elis Mandari Emerensia Patryconsitha Aman Emia Pepa Yosa Br Ginting Gede Widiantara Gunawan, I Wayan Nico Fajar Gusti Ayu Yuniati Kencana Gusti Made Widyantara Handayani, Ni Made Harjana, Ngakan Putu Anom Hendra Irawan I Gede Mahardika I Gede Raka Mas Tanaya I Gusti Agung Ayu Suartini I GUSTI AYU FITRI DIASTARI I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Kadek Swastika I Ketut Berata I Ketut Suada I Ketut Suada I Ketut Suada I Ketut Tomy Caesar Ramanda I Made Damriyasa I Made Dwinata I Made Gede Asta Ginawan I Made Gede Wijaya Kusuma I Made Kardena I Made Ricky Dwi Cahya I Made Subrata I Made Subrata I Made Subrata I MAde Sukada I Made Sukada I Made Sukada I Made Sukada I Made Sukada I Nengah Kerta Besung I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I Nyoman Bagus Tri Aribawa I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Agus Kertawirawan, I Putu Agus I Putu Ardiawan I Putu Sampurna I Wayan Batan I Wayan Masa Tenaya I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Polos I Wayan Suardana I Wayan Suardana I Wayan Sudarmayasa I Wayan Sudira I Wayan Wahyudi, I Wayan I Wayan Wirata I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Ayu Putu Aselya Mardyawati Ida Bagus Dimas Kusumadarma Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Krisna Pradnyadana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Ngurah Swacita Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Indraningrat, Anak Agung Gede Janice Girardi Janice Girardi Joanita Auxilia de Sa Pereira Julita Dewitri Merthayasa Kate Druhan Ketut Tono Pasek Gelgel Luh Made Sudimartini Luh Putu Pradnya Swari M.D. Rudyanto Made Damriyasa, Made Made Suma Anthara MAS DJOKO RUDYANTO MURNI SARI Natalia Natalia Ngakan Nyoman Cahaya Adi Ngakan Putu Anom Harjana Ngakan Putu Anom Harjana Ni Ketut Ayu Juliasih Ni Ketut Suwiti Ni Komang Dyah Purnamasari Ni Luh Gede Sudaryati Ni Made Ayudiningsih Astiti Sudewi Ni Nyoman Sri Budayanti Ni Putu Juni Ratna Dewi Ni Putu Tessa Arsaning Rahayu Ni Wayan Arya Utami Ni Wayan Arya Utami Ni Wayan Arya Utami, Ni Wayan Arya Nyoman Sadra Dharmawan Nyoman Sadra Dharmawan Nyoman Tigeh Suryadi Pande Putu Januraga Prabarini Hanum Sari Putra, I Made Maha Putri Destarani Putu Agus Trisna Kusuma Antara Putu Arya Suryanditha Putu Ayu Sisyawati Putriningsih Putu Erma Pradnyani Putu Henrywaesa Sudipa Putu Sasmitha Devi Cahyani Putu Velyniawati Ribka Listia Br Tarigan, Ribka Listia Romy Muhammad Dary Mufa Romy Muhammad Dary Mufa Sabelina Fitriani Samuel Mura Sang Gede Purnama Sang Gede Purnama sang gede purnama Segantara, Luh Gede Victoria Setiabudy, Marta Sonia Dewi Citra Sembiring Sosiawan, I Gede Arya Mas Sri Kayati Widyastuti Sudiartawan, I Putu Sukardika, Ketut Elok Sumarya, I Made Suputra, Gusde Wahyu Krisna Suryadi, Nyoman Tigeh Suwartama, Beny T. Sari Nindia Thea Sarassati, Thea Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjokorda Sari Nindhia TRI KOMALA SARI Uli Rehlitna Sembiring, Uli Rehlitna Vinensia Ghona Gani Widayantari, Anak Agung Ayu Sauca Sunia Widowati, I Gusti Ayu Rai - Wilson Lois, Wilson Yanti, Ni Komang Semara