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Kadek Karang Agustina
Department Of Veterinary Public Health, Faculty Of Veterinary Medicine, Udayana University, Denpasar, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Veteriner

 1 
Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.329 KB)

Abstract

This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Protective Immune Response of Post Rabies Vaccinated Dogs in Buduk Village, Mengwi, Badung, Bali (RESPONS IMUN PROTEKTIF PADA ANJING PASCAVAKSINASI RABIES DI DESA BUDUK, MENGWI, BADUNG, BALI) Ida Bagus Kade Suardana; Ida Bagus Oka Winaya; Desak Wiga Puspita Dewi; I Wayan Sudarmayasa; Kadek Karang Agustina
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.654 KB) | DOI: 10.19087/jveteriner.2019.20.2.264

Abstract

In efforts to eradicate rabies disease, vaccinations have been carried out targeting so-called rabies carrier animal such as dog andcat. Mass rabies vaccination has been done by the government of Bali annualy, in every sub-village at vaccination post or at every household. Minimum vaccination coverage to protect the threatened dog population from rabies is 70%. To determine whether the vaccinated dogs have protective antibody against rabies, this study have been done to find out the immune response of dogs after rabies vaccination in Buduk village. Sera were collected using random sampling in each subvillage, a total of 30 serum sample were examined, and the antibody was tested using kit ELISA Rabies®. Elisa test resulted in 90% of dogs have protective antibody titers (OD>0.5 EU). It concluded that dogs in Buduk village have a good immune response against rabies vaccine.
Dog Demography And Level Of Knowledge Against Rabies In Positive And Negative Case Of Rabies Areas In Mendoyo-Jembrana, Bali, Indonesia (DEMOGRAFI ANJING DAN TINGKAT PENGETAHUAN MASYARAKAT TERHADAP RABIES DI DAERAH DENGAN KASUS RABIES POSITIF DAN NEGATIF Kadek Karang Agustina; Putu Sasmitha Devi Cahyani; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.024 KB) | DOI: 10.19087/jveteriner.2017.18.4.642

Abstract

This research aims were to compare the demography of dogs and knowledge profile of dog owners against rabies in Mendoyo Sub-District, Jembarana Bali. This research used observational method, conducted from May to June 2016 in Mendoyo sub-district of Jembrana. Two Banjars were selected by the rabies case report in dogs (YehEmbangKangin village) and two other without case (MendoyoDanginTukad village). The data collected by interviewing the community and direct assessment of free-roaming dogs in the areas. A total of 200 household samples were taken, 50 respondents of each sampling area. This research resulted that the ratio of man-dog in Mendoyo was 4.5:1 and the ratio of male and female dogs was 1.8:1. As many as 64.8% of dog’s age were more than 12 month old, 28% were 4 to 12 month old and 8% were less than 4 month old. In the sampling areas, 19.4% of dogs were sterilized, which were 91.18% males and 8.82% females. Coverage of rabies vaccination in owned dogs was 82.3%. Dog’s petting method by people in Mendoyo was 69.71% free-roaming while 30.29% tied or caged. On free-roaming dog assessment, this study found 52 free-roaming dogs on the streets, 65.38% males and 34.62% females, only 30.8% of those used vaccination collar marker, Based on the body condition score, 76.9% of free-roaming dogs have good conditions. Of the total 200 households surveyed, there was a high level of knowledge about the danger of rabies and the importance of vaccination, but only a few people (<35%) received consulting about rabies, understood how to prevent rabies infection and the regulations about dog petting management. In positive area, we found there are still many people who have the habit of disposing dog. We conclude that there is needed to improve knowledge and attitude of the community about the prevention and risk factor of rabies.
Prevalence of Intestinal Worm in Free Ranging Domestic Cats in Bali (PREVALENSI CACING USUS PADA KUCING PELIHARAAN YANG BEBAS BERKELIARAN DI BALI) I Made Subrata; Ida Bagus Made Oka; Kadek Karang Agustina
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.504 KB) | DOI: 10.19087/jveteriner.2017.18.3.441

Abstract

The aims of this study were to identify and to measure the prevalence of intestinal worm infections in free-ranging domestic cats in Bali. As many as 133 cat fecal samples were collected from Bali and preserved in sodium acetic formaldehyde solution. Coproscopy method (sedimentation concentration and flotation techniques) was used to identify the eggs of helminthes. Based on fecal examination, eggs of four helminthes species : Toxocara, Ancylostoma, Cestoda and Capillaria were identified. This result indicates the prevalence of intestinal worms in free ranging domestic cats were high, consisting of Toxocara sp (71.43%), Ancylostoma sp (37.59%), Cestoda (19.55%) and Capillaria sp (0.75%). Therefore, it is needed to conduct programs to reduce and eradicate that helminthes. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi jenis cacing dan mengukur prevalensi infeksi kecacingan pada kucing yang diliarkan di Bali. Sebanyak 133 sampel feses kucing yang berasal dari seluruh Bali dikumpulkan dan disimpan dalam larutan sodium acetic formaldehide. Seluruh sampel diperiksa secara koproskopi dengan dua metode berbeda yaitu metode konsentrasi sedimentasi dan metode pengapungan untuk mengidentifikasi telur cacing yang terdapat pada feses kucing. Pada penelitian ini teridentifikasi empat jenis cacing yang menginfeksi kucing yang diliarkan di Bali yaitu Toxocara, Ancylostoma, Cestoda dan Capillaria. Hasil penelitian ini menunjukkan bahwa prevealensi infeksi kecacingan pada kucing yang diliarkan di Bali masih tinggi, yaitu Toxocara sp (71,43%), Ancylostoma sp (37,59%), Cestoda (19,55%) dan Capillaria sp (0,75%). Untuk itu diperlukan program pemberantasan dan pencegahan terhadap infeksi kecacingan pada kucing di Bali.
Kejadian Streptococcus suis pada Babi yang Dipotong di Rumah Pemotongan Hewan untuk Babi di Denpasar I Nengah Kerta Besung; Kadek Karang Agustina; I Gusti Ketut Suarjana; Ni Ketut Suwiti; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.525

Abstract

Streptococcosis cause by Streptococcus suis is an important bacterial disease because it has zoonotic potential and is capable of causing serious outbreaks in both pigs and humans. The main symptoms are meningitis, deafness, inflammation of the eyes to blindness. This study aims to find the presence of S. suis bacteria infection on slaughtered pigs in Denpasar. A total of 200 pig tonsils were taken as samples with 20 sampling times. Each sampling was taken 10 samples. The samples were grown on 5% sheep blood agar then followed by Gram staining, oxidase, coagulase, and catalase test. Suspected positive results in each collection were continued with the Polymerase Chain Reaction primers SSRecN-F and SSRecN-R tests. The results of testing on 200 samples of pig tonsils showed that 2.5% of samples were positive for S. suis. Based on the results of the study, it can be concluded that the sample of pig tonsils from the pig slaughterhouse in Denpasar was infected with S. suis.
Co-Authors Adhiputra, I Ketut Agus Indra Adiwinata, Putu Diva Ady Fendriyanto, Ady Aida Lousie Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Dharmayudha Anak Agung Komang Suardana Andriawino Berdionis Sanam Anne Dawdoya Cesarina Pascalia dos Santos Alegria Desak Wiga Puspita Dewi Desy Ariani Ferdianti, Desy Ariani Dewa Made Dwi Parwata Dimas Indra Dwi Purnama DWI SURYANTO Elis Mandari Emerensia Patryconsitha Aman Gunawan, I Wayan Nico Fajar Gusti Ayu Yuniati Kencana Gusti Made Widyantara Handayani, Ni Made Harjana, Ngakan Putu Anom Hendra Irawan I Gede Mahardika I Gede Raka Mas Tanaya I Gusti Agung Ayu Suartini I GUSTI AYU FITRI DIASTARI I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Kadek Swastika I Ketut Berata I Ketut Suada I Ketut Suada I Made Damriyasa I Made Dwinata I Made Gede Wijaya Kusuma I Made Kardena I Made Ricky Dwi Cahya I Made Subrata I Made Subrata I Made Subrata I Made Sukada I Made Sukada I Nengah Kerta Besung I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Sampurna I Wayan Batan I Wayan Masa Tenaya, I Wayan Masa I Wayan Polos I Wayan Suardana I Wayan Sudarmayasa I Wayan Wahyudi, I Wayan I Wayan Wirata I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Ayu Putu Aselya Mardyawati Ida Bagus Dimas Kusumadarma Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Krisna Pradnyadana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Ngurah Swacita Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Indraningrat, Anak Agung Gede Janice Girardi Janice Girardi Julita Dewitri Merthayasa Kate Druhan Luh Made Sudimartini Luh Putu Pradnya Swari M.D. Rudyanto Made Damriyasa, Made Made Suma Anthara MAS DJOKO RUDYANTO MURNI SARI Natalia Natalia Ngakan Putu Anom Harjana Ngakan Putu Anom Harjana Ni Ketut Ayu Juliasih Ni Ketut Suwiti Ni Luh Gede Sudaryati Ni Made Ayudiningsih Astiti Sudewi Ni Nyoman Sri Budayanti Ni Putu Tessa Arsaning Rahayu Ni Wayan Arya Utami Ni Wayan Arya Utami Ni Wayan Arya Utami, Ni Wayan Arya Nyoman Sadra Dharmawan Nyoman Tigeh Suryadi Pande Putu Januraga Prabarini Hanum Sari Putra, I Made Maha Putu Agus Trisna Kusuma Antara Putu Arya Suryanditha Putu Ayu Sisyawati Putriningsih Putu Erma Pradnyani Putu Henrywaesa Sudipa Putu Sasmitha Devi Cahyani Putu Velyniawati Ribka Listia Br Tarigan, Ribka Listia Sabelina Fitriani sang gede purnama Sang Gede Purnama Sang Gede Purnama Setiabudy, Marta Sonia Dewi Citra Sembiring Sosiawan, I Gede Arya Mas Sri Kayati Widyastuti Sudiartawan, I Putu Sukardika, Ketut Elok Sumarya, I Made Suputra, Gusde Wahyu Krisna Suryadi, Nyoman Tigeh Suwartama, Beny T. Sari Nindia Thea Sarassati, Thea Tjokorda Sari Nindhia TRI KOMALA SARI Uli Rehlitna Sembiring, Uli Rehlitna Vinensia Ghona Gani Widayantari, Anak Agung Ayu Sauca Sunia Widowati, I Gusti Ayu Rai - Wilson Lois, Wilson Yanti, Ni Komang Semara