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All Journal Journal of Business Strategy and Execution Jurnal Biosains Pascasarjana Indonesian Journal of Pharmaceutical Science and Technology Jurnal Biologi Tropis Jurnal Riset Akuakultur Jurnal PROTEKSI (Proyeksi Teknik Sipil) Jurnal Pengabdian Magister Pendidikan IPA Jurnal Gema Ngabdi IJAF Bioscientist : Jurnal Ilmiah Biologi Tsarwah : Jurnal Ekonomi dan Bisnis Islam Iqtishaduna : Jurnal Ilmiah Mahasiswa Hukum Ekonomi Syariah Journal of Science and Science Education (JoSSEd) Journal of Agritechnology and Food Processing Journal of Economics Review (JOER) Development Policy and Management Review Journal of Entrepreneurship and Community Innovations Indonesian Proceedings and Annual Conference of Islamic Law And Sharia Economic (IPACILSE) Media Hukum Indonesia (MHI) Journal of Fisheries & Marine HYPOTHESIS : MULTIDISCIPLINARY JOURNAL OF SOCIAL SCIENCES Jurnal Agripet Al-Ahkam: Jurnal Ilmu Syari'ah dan Hukum International Journal of Science, Technology and Applications Fundamental : Jurnal Ilmiah Hukum Smart Humanity: Jurnal Pengabdian Masyarakat Interdisciplinary Explorations in Research Journal (IERJ)
Muhamad Amin
Alumni of BINUS BUSINESS SCHOOL, BINUS UNIVERSITY, JWC Campus, Jl. Hang Lekir I No. 6, Kebayoran Baru, South Jakarta 12120
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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EFFECT OF DIFFERENCES BROMELAIN ENZYME CONSENTRATION ON PROTEIN HYDROLYSATE FROM WASTE OF TILAPIA VISCERA (Oreochromis sp.) ON ANTIOXIDANT ACTIVITY Muhammad Athoillah Sholahuddin; Lastuti, Nunuk Dyah Retno; Amin, Muhamad
Jurnal Biosains Pascasarjana Vol. 26 No. 1 (2024): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v26i1.2024.15-22

Abstract

Tilapia is a commodity that is widely consumed by the public, especially in Indonesia. Fresh tilapia offal has a protein content composition of 55.55%, so to increase its selling value it is often made into protein hydrolysate. Protein hydrolysis using enzymes is an effective way, because it can make protein hydrolysates avoid damage to certain amino acids. Proteolytic enzymes have many types and one that can be used as a hydrolyzer, in producing protein hydrolysates is bromelain enzyme. The purpose of this study was to determine the effect of proteolytic enzyme type and hydrolysis time on antioxidant activity of protein hydrolysate from Oreochromis sp. offal waste. This study used RAL 2 factors and each was repeated 3 times. The first factor was the concentration of bromelain enzyme used, namely control concentration, 1%, 2% and 3%. The second factor was the hydrolysis time, which was 4 hours, 6 hours and 8 hours. The data obtained were analyzed and continued with antioxidant activity test, hydrolysis degree test and protein content test. The best concentration of bromelain enzyme as antioxidant was 3% concentration for 6 hours hydrolysis with IC50 value of 82.53µg/mL, the best DH of 82.25%±4.03 and the highest protein content of 54.55%. Keywords: Antioxidant, bromelain enzyme, tilapia offal, protein hydrolysate
Application of Cellulase Enzyme in Feed on the Growth of Siamese Catfish (Pangasius hypophthalamus) Anjasmara, L. Dede Arta Satya; Amin, Muhamad; Mukti, Akhmad Taufiq; Mahasari, Gunanti; Agniny, Siswi Fililmi; Ali, Muhamad
Journal of Science and Science Education Vol. 6 No. 2 (2025): October
Publisher : Pascasarjana, Mataram University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jossed.v6i2.11894

Abstract

Siamese catfish (Pangasius hypophthalamus) is one of the most economically importantfreshwater commodities in Indonesian aquaculture. A major challenge during the growth-out phase, is the limited ability to digest plant-based diets due to high cellulose and crude fiber content, which reduce feed digestibility. Supplementation of cellulase in feed has been proposed as a strategy to improve nutrient utilization. Therefore the present study investigated the effect of dietary celulase supplementation on feed digestibility and growth performance in Seamese catfish. Juvenile catfish with an average initial length of 6cm were reared for 30 days in floating net in a cement pond. A complete reandomized design was employed with four treatments, two control, and four replciates. Treatments included cellulase derived from mivrobes sources T1 = Plant feed + WT, T2 = Plant feed + (WT + SKIK), T3 = Plant feed + Mutant, T4 = Plant feed + (mutant + SKIK). The control consisted of C1 = Commercial feed and C2 = Plant-based feed without enzyme supplementation. The results showed that C1 showed the best results for the growth of fish. However, T2, T3 and T4 showed the best results when compared to C2 and T1 which used 100% vegetable protein feed. Therefore, it can be concluded that the addition of cellulase enzymes to the feed has an effect on increasing the growth of Siamese catfish.
Production, Diversity, and Distribution of Aquaculture Commodities in Tuban, Indonesia Samara, Syifania Hanifah; Amin, Muhamad; Isroni, Wahyu; Hasan, Veryl; Santanumurti, Muhammad Browijoyo; Nugraha, Muhammad Ar Rozzaaq; Abualreesh, Muyassar H.
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.54608

Abstract

Graphical Abstract Highlight Research Tuban (Indonesia) aquaculture commodities are surveyed. Aquaculture diversity in Tuban are consisted of 5 fields, namely rice field ponds, floating net cages, freshwater ponds, and brackish aquaculture ponds Aquaculture commodities in Tuban consist of 11 families and 14 species of the main aquatic production. Cyprinus carpio or common carp is one of the commodities cultivated in four different aquacultures. Abstract Fishery is a sector expected to overcome the food crisis. Indonesia is one of the leading countries in high volumes of fishery production in most of its areas, including the Regency of Tuban in East Java Province. The area of Tuban Regency is 1,839.94 km2 with the coastline covers 65 km and a sea area of 22,608 km2. This study aims to provide information regarding aquaculture production, diversity, and distribution in Tuban.  This study used a survey method in collecting fishery commodity data directly from aquaculture areas. The results of this study showed that the highest production came from the brackish water with a value of 32.46% (13,561.79 tons), followed by rice field pond (27.79% or 11,612.45 tons), freshwater pond (25.76% or 10,764.62 tons), floating net cage (13.97% or 5,836.59 tons), and marine (0.1% or 5.67 tons). Aquaculture biodiversity in Tuban consisted of 11 families and 14 species of the main aquatic produces, such as Cyprinidae (Cyprinus carpio, Barbonymus gonionotus, Parastromateus niger), Clariidae (Clarias bathracus), Pangasiidae (Pangasianodon hypophthalmus), Penaeidae (Penaeus monodon, Litopenaeus vannamei), Channidae (Channa striata), Cichlidae (Oreochromis niloticus, Oreochromis mosammbicus), Osphronemidae (Osphronemus gourami), Serranidae (Epinephelus), Lutjanidae (Lutjanus), and Chanidae (Chanos chanos). Cyprinus carpio or common carp is fish cultivated in four different aquacultures, namely rice field pond, floating net cages, freshwater ponds, and brackish aquaculture ponds. Unfortunately, the diversity and production of marine aquaculture has not been improved even though Tuban has large marine areas.
Analisis Molekuler Tilapia Lake Virus (TiLV) di Sentra Budidaya Benih Ikan Gurami (Osphronemus goramy) Jawa Timur Jumria, Andi; Amin, Muhamad; Mufasirin, Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9515

Abstract

Tilapia Lake Virus (TiLV) is a New Emerging Disease that has been defined and included in the WOAH disease list (WOAH, 2022). This virus can infect both freshwater and marine fish, causing high mortality in Ttilapia up to 90%. Reverse transcyptase method - Semi Nested Polymerase Chain Reaction (RT-snPCR) for detection of TiLV in Osphronemus goramy. TiLV was discovered for the first time in Thailand and was also found in Jogjakarta in 2021. Further research is needed to see the damage and genetic variation caused by TiLV in Osphronemus goramy. This study aims to study the genetic variability and homology of TiLV isolates in Osphronemus goramy as definitive hosts in East Java. The results showed clinical symptoms of lethargic fish, decreased appetite, abnormal behavior, reduced reflexes, sluggish movements, darker body color, congestion, thinning fins and exopthalmia. The PCR results showed that the Osphronemus goramy showing clinical symptoms of TiLV infection were all positive. The sequencing results were further identified using the BLAST program, showing the highest similarity of the genetic identity of the sample at 96.8% with TiLV from Israel (Genebank Accession Number NC 029927.1).
Antisipasi Kekerasan Dalam Rumah Tangga (KDRT) di Desa Pandai, Kabupaten Bima, NTB: Peningkatan Kesadaran dan Pemberdayaan Masyarakat Amin, Muhamad; Murdiono, Murdiono
Journal of Entrepreneurship and Community Innovations Vol 2 No 2 (2024): FEBRUARI 2024
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jeci.v2i2.157

Abstract

Tujuan dari pengabdian ini adalah untuk meningkatkan kemandirian anak-anak di Panti Asuhan Ulil Abshar Dau Sengkaling Malang melalui program kegiatan mandiri dengan pendekatan pendidikan dan pembinaan. Metode yang digunakan meliputi penyusunan program kegiatan mandiri yang mencakup berbagai keterampilan, pelatihan, serta pembinaan secara individu dan kelompok. Hasil dari pengabdian ini menunjukkan bahwa pendekatan pendidikan dan pembinaan telah memberikan dampak positif dalam meningkatkan kemandirian anak-anak di panti asuhan tersebut. Anak-anak berhasil mengembangkan keterampilan teknis, kewirausahaan, dan keterampilan sosial melalui program kegiatan mandiri. Selain itu, keterlibatan keluarga dalam program juga menciptakan lingkungan dukungan yang lebih luas bagi anak-anak, meningkatkan rasa percaya diri dan kemandirian mereka. Kesimpulannya, program "Peningkatan Kemandirian Anak Panti Asuhan Ulil Abshar Dau Sengkaling Malang melalui Program Kegiatan Mandiri: Pendekatan Pendidikan dan Pembinaan" berhasil memberikan kontribusi yang signifikan dalam membantu anak-anak panti asuhan untuk menjadi lebih mandiri dan siap menghadapi tantangan di masa depan. Diperlukan komitmen dan dukungan yang berkelanjutan untuk memastikan kelangsungan program ini dan manfaatnya bagi anak-anak panti asuhan.
Pelatihan Pembuatan Permen Jilat Sapi Sebagai Pakan Suplemen Pada Kelompok Pemuda Tani Ternak Maju Bersama Di Desa Penyaring Kecamatan Moyo Kabupaten Sumbawa Noersidiq, Azhary; Dilaga, Syamsul Hidayat; Amin, Muhamad; Yanuarianto, Oscar; Suhubdy, Suhubdy; Dahlanuddin, Dahlanuddin; Fahrullah, Fahrullah; Maslami, Vebera
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.4783

Abstract

The partner involved in community service activities is the Maju Bersama Livestock Farmer Youth Group in Desa Penyaring Kecamatan Moyo Utara Kabupaten Sumbawa. The problems faced are that farmers are very dependent on the availability of forage feed but its availability is limited during the dry season and the lack of knowledge of farmers regarding the adequacy of feed nutrition to the needs of livestock and the lack of innovation of farmers to utilize or process local feed ingredients and agricultural waste that can be made as alternative feed and feed supplements. The purpose of this activity is to help farmers to increase cattle productivity by utilizing local feed ingredients from available agricultural waste and processed in the form of supplementary feed is "Permen Jilat Sapi". The activity method includes counseling on making cow lick candy and direct training for farmers. The results of the activity were that the cow lick candy produced was classified as good with a firm texture and not soft and fragrant with the distinctive smell of molasses and corn. It was concluded that overall, the farmers' understanding of Permen Jilat Sapi after the training ranged from 80%-90%. This can be used as an indication that the activity has been successful and received a good response from farmers and as many as 75% of participants are willing to apply this feed supplement for their livestock.
Quality Improvement of the Ammoniated Corn Husk through the Use of MA-11 Inoculant at Different Doses Amin, Muhamad; Putra, Ryan Aryadin; Yanuarianto, Oscar; Noersidiq, Azhary; Suhubdy, Suhubdy; Dilaga, Syamsul Hidayat
Jurnal Agripet Vol 25, No 2 (2025): Volume 25, No. 2 October 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i2.45634

Abstract

The research objective was to study the effect of different doses of MA-11 inoculants on improving the quality of ammoniated corn husks. The materials used were corn husks, urea, and the MA-11 inoculant. The research was conducted using Completely Randomized Design (CRD) with four treatments and three replications. The four treatments were T0: Ammoniated corn husks + 0% MA-11 (control), T1: Ammoniated corn husks + 0.5% MA-11, T2: Ammoniated corn husks + 1% MA-11, and T3: Ammoniated corn husks + 1.5% MA-11. The observed variables were chemical composition (dry matter, organic matter, crude protein, crude fiber, ether extract, and TDN), fiber components (NDF, ADF, cellulose, hemicellulose, and lignin), and in-vitro digestibility (dry matter and organic matter digestibility). The data obtained were analyzed by analysis of variance and further tested by Duncan's multiple range test. The results showed that the treatments had significantly different effects (P0.05) on chemical composition, fiber fractions, and digestibility. The 0.5% (T1) dosage rate yielded the best results for CP (7.25%), and TDN (56.88%); and achieved the greatest reduction in CF (28.57%) and ADF (42.90%) content. Conversely, the 1.5% (T3) dosage rate was optimal for EE (1.16%), DMD (43.23%), and OMD (43.90%). While all inoculation levels (0.5% to 1.5%) were also effective in reducing the NDF, cellulose, hemicellulose, and lignin fractions, whereas the highest (P0.05) Dry Matter and Organic Matter content was observed in the control (T0). It was concluded that different doses of the MA-11 inoculant had varied and specific effects on the nutritional parameters of ammoniated corn husk.
Sosialisasi Terkait Larangan Praktik Penipuan Dan Iklan Menyesatkan Oleh Pelaku Usaha Dalam Perlindngan Konsumen Kepada Mahasiswa UIN Antasari Banjarmasin Rahmaniah, Amelia; Mahmudah, Mahmudah; Annisa, Nia; Nazla, Siti; Hafizah, Silva Nor; Jannah, Nadyatul; Amin, Muhamad; Ikhlas, Muhammad; Rafe’i, Muhammad Khairur; Hakim, Muhammad Azizul; Syaupi, Muhammad; Rahman, Aulia
Interdisciplinary Explorations in Research Journal Vol. 3 No. 3 (2025)
Publisher : PT. Sharia Journal and Education Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62976/ierj.v3i3.1553

Abstract

Perkembangan media digital meningkatkan praktik penipuan dan iklan menyesatkan yang merugikan konsumen. Kurangnya pemahaman masyarakat, khususnya mahasiswa, terhadap hak konsumen menyebabkan perlindungan konsumen belum berjalan optimal sesuai Undang-Undang Nomor 8 Tahun 1999. Kegiatan pengabdian masyarakat ini bertujuan memberikan pemahaman kepada mahasiswa mengenai praktik penipuan dan iklan menyesatkan serta hak konsumen. Metode yang digunakan adalah sosialisasi melalui pemaparan materi dan sesi tanya jawab kepada mahasiswa UIN Antasari Banjarmasin. Hasil kegiatan menunjukkan adanya peningkatan pemahaman mahasiswa tentang ciri iklan menyesatkan dan sikap lebih hati-hati sebagai konsumen.
PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF TENGKAWANG BUKIT (Shorea beccariana) STEM EXTRACT AGAINST Vibrio parahaemolyticus Anisa, Hosia; Mukti, Akhmad Taufiq; Mahasri, Gunanti; Amin, Muhamad; Aminah, Nanik Siti
Jurnal Riset Akuakultur Vol 20, No 3 (2025): September (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.3.2025.245-255

Abstract

Vibrio parahaemolyticus infection poses a significant challenge in intensive shrimp culture, leading to substantial economic losses. Its common treatments using synthetic antibiotics have been linked to increased risks of antibiotic resistance and residual effects. Therefore, finding environmentally safe and effective natural alternatives is deemed essential. Tengkawang Bukit (Shorea beccariana) stem extract contains antibacterial compounds, including asiatic acid, oleanolic acid, and lupanone, all classified as terpenoids. This study aims to evaluate the antibacterial potential of S. beccariana stem extract through phytochemical screening and a disc diffusion test against V. parahaemolyticus. The screening results confirmed the presence of bioactive compounds, including alkaloids, flavonoids, saponins, tannins, and steroids. The disc diffusion test showed an increase in the inhibition zone with increasing extract concentration, with inhibition diameters ranging from 9.04 ± 0.48 mm to 10.75 ± 0.26 mm. The 17% extract yield indicates a high availability of active compounds. These findings suggest that tengkawang bukit stem extract has potential as a natural antibacterial alternative for controlling V. parahaemolyticus in shrimp culture. This approach could help reduce reliance on synthetic antibiotics and promote sustainable fisheries. Infeksi Vibrio parahaemolyticus merupakan salah satu kendala utama dalam budidaya udang yang menyebabkan kerugian ekonomi signifikan. Penggunaan antibiotik sintetis sebagai pengobatan telah menimbulkan kekhawatiran terkait resistensi dan residu, sehingga diperlukan alternatif alami yang aman dan efektif. Batang tengkawang bukit (Shorea beccariana) diketahui mengandung senyawa antibakteri seperti asam asiatik, asam oleanolik, dan lupanon yang tergolong ke dalam senyawa terpenoid. Studi ini bertujuan untuk mengevaluasi potensi antibakteri ekstrak batang S. beccariana melalui skrining fitokimia dan uji difusi cakram terhadap V. parahaemolyticus. Hasil skrining menunjukkan adanya kandungan senyawa bioaktif berupa alkaloid, flavonoid, saponin, tanin, dan steroid. Uji difusi cakram memperlihatkan zona hambat yang meningkat seiring dengan peningkatan konsentrasi ekstrak, dengan diameter hambatan berkisar 9,04 ± 0,48 mm hingga 10,75 ± 0,26 mm. Rendemen ekstrak sebesar 17% menunjukkan ketersediaan senyawa aktif yang melimpah. Hasil riset ini mengindikasikan bahwa ekstrak batang tengkawang bukit berpotensi sebagai alternatif antibakteri alami dalam pengendalian V. parahaemolyticus pada budidaya udang, sehingga dapat mengurangi ketergantungan terhadap antibiotik sintetis dan mendukung keberlanjutan perikanan.
PENINGKATAN AKTIVITAS DAN EKSPRESI PROTEIN TOTAL ENZIM SELULASE REKOMBINAN SERTA POTENSI APLIKASINYA PADA PAKAN IKAN Agniny, Siswi Fililmi; Mahasri, Gunanti; Mukti, Akhmad Taufiq; Amin, Muhamad
Jurnal Riset Akuakultur Vol 20, No 3 (2025): September (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.3.2025.257-267

Abstract

Meskipun enzim selulase rekombinan banyak dikembangkan untuk meningkatkan efisiensi pemecahan serat, informasi mengenai bagaimana modifikasi seperti mutasi dan penambahan tag SKIK (Ser-Lys-Ile-Lys) dapat memengaruhi aktivitas enzim dan produksi protein total masih terbatas. Oleh karena itu, penelitian ini bertujuan mengevaluasi pengaruh mutasi dan penambahan tag SKIK terhadap aktivitas dan protein total enzim selulase rekombinan yang diproduksi oleh Escherichia coli BL21 (DE3). Penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan, yaitu P1= wild type (WT), P2 = WT + SKIK, P3 = mutan, dan P4 = mutan + SKIK, masing-masing dengan empat ulangan. Produksi enzim dilakukan melalui kultur E. coli rekombinan yang membawa plasmid pET-22b-CellE dengan induksi laktosa monohidrat, kemudian dianalisis menggunakan uji aktivitas selulase metode 3,5-dinitrosalisilat (DNS) dan pengukuran protein total dengan metode Bradford. Data dianalisis menggunakan ANOVA satu arah dan uji lanjut Duncan pada tingkat signifikansi 5% (p < 0,05). Hasil penelitian menunjukkan bahwa perlakuan mutan + SKIK menghasilkan nilai tertinggi baik pada aktivitas enzim (10,66 U mL-1) maupun protein total (0,85 mg mL-1). Penambahan SKIK meningkatkan ekspresi dan kelarutan protein, sedangkan mutasi memperbaiki efisiensi katalitik enzim. Kombinasi keduanya memberikan efek sinergis dalam meningkatkan perfoma enzim selulase rekombinan. Penelitian ini merupakan yang pertama mengombinasikan penambahan tag SKIK dengan mutagenesis untuk meningkatkan ekspresi dan aktivitas katalitik enzim selulase pada E. coli. Temuan ini tidak hanya relevan untuk peningkatan produksi enzim pada industri bioteknologi, tetapi juga berpotensi diaplikasikan dalam formulasi pakan ikan. Although recombinant cellulase enzymes have been widely developed to enhance fiber-degradation efficiency, information regarding how modifications such as mutagenesis and the addition of the SKIK (Ser-Lys-Ile-Lys) tag influence enzymatic activity and total protein production remains limited. Therefore, this study aimed to evaluate the effects of mutagenesis and SKIK tagging on the activity and total protein yield of recombinant cellulase expressed in Escherichia coli BL21 (DE3). The research was conducted experimentally using a completely randomized design (CRD) with four treatments: P1 = wild type (WT), P2 = WT + SKIK, P3 = mutant, and P4 = mutant + SKIK, each consisting of four replications. Enzyme production was carried out using recombinant E. coli harboring the pET-22b-CellE plasmid induced with lactose monohydrate, and the resulting enzyme was analyzed for cellulase activity using the 3,5-dinitrosalicylic acid (DNS) method and total protein concentration using the Bradford assay. Data were analyzed using one-way ANOVA followed by Duncan’s post-hoc test at a 5% significance level (p < 0.05). The results showed that the mutant + SKIK treatment produced the highest values for both enzymatic activity (10.66 U mL-1) and total protein (0.85 mg mL-1). The addition of the SKIK tag enhanced protein expression and solubility, whereas mutagenesis improved catalytic efficiency. The combination of both modifications produced a synergistic effect, resulting in superior performance of the recombinant cellulase enzyme. This study is the first to combine SKIK tagging with mutagenesis to enhance the expression and catalytic activity of cellulase in E. coli. These findings are not only relevant for improving enzyme production in biotechnology industries but also hold potential applications in fish feed formulation.
Co-Authors Abdul Malik Abualreesh, Muyassar H. Agniny, Siswi Fililmi Agus Gunawan Ahmad A Ahmad Taufik S Akbar, Fauzan Akhmad Taufiq Mukti Akhyar Alawiyah, Geby Almatini, Soviliana Amelia Rahmaniah Amrullah, Shafwan Anisa, Hosia Anjasmara, L. Dede Arta Satya Annisa, Nia Aulia Rahman Azhary Noersidiq Cahyawati, Triyana Dyah Dahlanuddin Dahlanuddin, Dahlanuddin Elvina, Ina Fahrullah Fahrullah Febriarsita Eka Sasmita GOMIES, STEVANUS JOHAN Gufran Gunanti Mahasri Hafizah, Silva Nor Hakim, Muhammad Azizul Handayana, I Gusti Ngurah Yudi Hasnah Hasnah Hatuwe, Rahma Satya Masna Haudh Al Maa'uun Husnawati Husnawati Iksan Imaduddin, M Asad Imam Bukhari Imran, Tya Isroni, Wahyu J, Jufrin Jannah, Nadyatul Jayadi, Edi M Jumria, Andi Juwaidin, Juwaidin Kurniawidi, Dian W Lilianti, Mita Ayu Liliyanti, Mita Ayu Mabruroh, Ahda Inayati Mahasari, Gunanti Mahmud Syukri Mahmudah Mahmudah Matakita, Muhajir Mufasirin Muhamad Ali Muhammad Athoillah Sholahuddin Muhammad Ikhlas Munir Murdiono Murdiono, Murdiono Musa, Muhammad Nur Denny Musa, Muhammad Nur Deny Muspiha, Muspiha Musthofa, Abdul Halim N, Nasrullah Nadila, Nadila Nanik Siti Aminah Nasution, Adnan Nazla, Siti Nugraha, Muhammad Ar Rozzaaq Nunuk Dyah Retno Lastuti Oktosuyono, Oktosuyono Oscar Yanuarianto, Oscar Purnaning, Dyah Putra, Ryan Aryadin Rafe’i, Muhammad Khairur Richard Kumaradjaja Robby, Robby Sagaf, Umar Salonten, Salonten Samara, Syifania Hanifah Santanumurti, Muhammad Browijoyo Saragi, Robson Sarimov, Renat Siti Zahra Khoirunnisa Siwabessy, Dominggus J Siwabessy, Dominggus Jacobus Ssonko Muhammedi Suhubdy Suhubdy Sukirman Sukirman Sulaeman Jajuli, Sulaeman Surijadi, Herman Susi Rahayu Syaeful Bahri Syamsul Hidayat Dilaga, Syamsul Hidayat Syaupi, Muhammad Tousalwa, Carla Carolien Vebera Maslami Veryl Hasan Wahyudinsyah Wattiheluw, Siti Yayu Wattimena, Muhamad Amin Widodo, Dwi Candra Yuli Kusuma Dewi