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Journal : journal of Basic Medical Veterinary

PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL SERTOLI MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Berlina, Cyrcilia Relita; Eliyani, Hana; Sami, Abdul; Widjiati, Widjiati; Mulyati, Sri; Anwar, Chairul
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19991

Abstract

This study  was aimed  to know  the effect of Kebar grass  extract  on amount of mice  Sertoli  cell exposed  by TCDD.  This study was  experimental   study  with completely   randomized  design.  Thirty adult  male  mice  of Mus  musculus strain Balb/C,  age 11 weeks  and  weight   25 - 30 ~were    used.  Mice were divided   into five groups,   that  were:  K- was  control  (aquadest   0)   mL); K+ was  injected  with TCDD  at  dose  of 7 µg/kg   BW;  Pl  was  injected  with TCCD  and  administrated with   Kebar   extract   (0,045 mg/  g  BW/day);   P2 was   injected   with   TCCD  and administrated  with  Kebar  extract  (0,080 mg/ g BW/day);   P3 was  injected  with TCDD and  administrated with  Kebar extract  (0,135 mg/  g BW/day).   Kebar grass extract  was  administrated in 53 days.  Mice were sacrificed  and  right  testis organs were  taken.  Then, histology   preparat  with  HE  staining   were  made  and  Sertoli cells  were  counted.    Data were   analyzed  by  One  Way  ANOV A  followed   by Duncan test  (a  =  0,05).   The result  of this  study   showed that  administration  of Kebar  grass  extract  affected  amount  of mice sertoli  cells.  K+ (2,92a±0,109) showed significance difference  (p<0,05) compared   to Pl (5,00b±0,374), P2 (7,64c±0,409)  and P3 (9,68ct±0,363).  Meanwhile, amount   of Sertoli cells of P3 at the highest  dose  did not  show  significance difference   (p>0,05)  with  K-  (10,16<l±0,829).  The conclusion of this study was  administration of Kebar  grass  extract  per  oral in 53 days  could maintain amount  of mice Sertoli cell exposed  by TCDD. 
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST HISTOPATHOLOGICAL CHANGES OF SMALL INTESTINE ON MALE WHITE RAT (Rattus norvegicus) WISTAR STRAIN Amaris, Aulia Puspa; Yustinasari, Lita Rakhma; Legowo, Djoko; Plumeriastuti, Hani; Anwar, Chairul; Hidajati, Nove
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20409

Abstract

This study was conducted to investigate the effect of polypropylene residue on heated palm oil against histopathological changes of small intestine on male white rat (Rattus norvegicus) wistar strain. Eighteen male white rats divided into three groups and were administered via peroral gavage with different treatments for five weeks. The treatment were Control (84 grams palm oil), P1 (84 grams palm oil with 2.3 grams plastic), and P2 (84 grams palm oil with 6.81 grams plastic). The histopathological changes of small intestine were examined by using Barthel Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed that were significant difference (p<0.05) between treatment groups. The worse damage of polypropylene plastic residue was 6.81 grams.
HISTOPATHOLOGICAL CHANGES OF KIDNEY OF BROILER CHICKEN EXPOSED TO CHRONIC HEAT STRESS Xin, Irene Teh Kai; Plumeriastuti, Hani; Anwar, Chairul; Rachmawati, Kadek; Utama, Suzanita; Legowo, Djoko
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20411

Abstract

The aim of this research was to know histopathological changes of kidney of broiler chicken exposed to chronic heat stress. Twenty broilers were divided randomly into 2 groups, 10 broilers each are being exposed to chronic heat stress for 21 days. The first 21 days were the adaptation period in a chamber with temperature 24-28o C and humidity 40-55%. After 21 days, continue with exposure to heat stress in a chamber with temperature 36-40o C and humidity 50-65% in 8 hours per day. After exposed to chronic heat stress, kidney tissues were processed, and kidney tissue histopathological changes were evaluated by using the Klopfleisch modified scoring method. The data was analyzed by Mann – Whitney Test. The result of this research showed that chronic heat stress exposure causing the presence of degeneration of tubular epithelial cell, necrosis of tubular epithelial cell, necrosis of glomerular and interstitial infiltration. From the analysis data the overviewof multiparametric showed that when control group compared with the treatment group showed significantly difference (p<0.05). 
KEMAMPUAN MALARISIDAL NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI TERAPI AJUVAN PADA HISTOPATOLOGI HATI MENCIT (Mus musculus) PENDERITA MALARIA Pujiyanto, Mahendra; Maslachah, Lilik; Triakoso, Nusdianto; Lazuardi, Mochamad; Anwar, Chairul; Legowo, Djoko
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21094

Abstract

The aimed of this study is to know malaricidal ability of nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy on liver histopathological of mice (Mus musculus) which infected by Plasmodium berghei. This research uses 30 mice which are divided into six treatment groups namely K0 (not infected without treated), K+ (infected without treated), K1 (infected and treated with chloroquin), K2 (infected and treated with juwet leaf extract), P1 (infected and treated with juwet leaf extract nanoparticle) and P2 (infected and combination treated with chloroquine and juwet leaf extract nanoparticle) which infected by 1x105 in 0.2 ml of Plasmodium berghei. The data were analyzed by Kruskal Wallis using SPSS and followed with Mann-Whitney test. This research result showed there was significant difference (p < 0.05) between K1 and P2, but did not showed significant difference (p > 0.05) with K+, K2 and P1. Based on this research, it could be concluded that nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy estimated can increase malaricidal ability in degeneration, necrosis, portal inflammation, Kupffer cell hyperplasia, sinusoidal congestion, and hemosiderosis in mice liver which infected by Plasmodium berghei.
Co-Authors Aat Ruchiat Nugraha Achmad Asrori Achmad Ruslan Afendi Adinda, Catur Putriyani Charisma Adyt Anugrah Agung Herubawa, Dio Agus Jatmiko Agus Pahrudin Ahmad Ridani Ahmad Yani Ahyani, Edi Aisyah, Ai Al-Bawazir, Syifa Alfusanah, Fadilatul Amaris, Aulia Puspa Andriansah, Zjulpi Ani Cahyadi Anis Masykhur Anjarsari, Elly Antomi Saregar Apriliano, Amanda Arbain Tata Aziz, Muhammad Ja'far Abdul Aznem, Ahmad Bachrie Tanrere, Syamsul Baharudin Baharudin Berlina, Cyrcilia Relita Boukhedimi, Chems Eddine BUNGA, SRI Burhan, Nurmala Citra Dewi, Desy Daga, Agustinus Tanggu Deden Makbuloh Desiana Desiana, Desiana Dewi, Desy Citra Effendy A, Sahrul Eka Puspita Sari Elfrida Ratnawati Erlangga, Ade Fachry Abda El Rahman Fahrur Rozi Faisal Faisal Fathurrohman, Asep Fatimah, Aulia Fikri Maulana, Fikri Fitro Darwis Fuja Siti Fujiawati, Fuja Siti Gaus, Abdul Ghifari, Dimas Al Gunawan, Reno Guntur Cahaya Kesuma Gymnastiar, Alfarisi Halimatusakdiah, Halimatusakdiah Hana Eliyani Hani Plumeriastuti Harahap, Amin Hasanah, Rina Hasbar, Hasbar Herdiansyah, Gilang Heru Juabdin Sada Hidajati, Nove Ibnu Austrindanney Sina Azhar Idha Silviyati Irwandani Irwandani Ishaki, Subhi Nur Iwan Sahrial Hamid Jagat, Lalu Surya Jamal Fakhri Jodian Saputra, Shadam Kadek Rachmawati Karmilah, Karmilah Kasturi, Rima Kausari, Ahmad Kesuma, Agus Heri Kesuma, Muhammad Ilham Jaya Kusumaningrum, Putri Anggraheni Laili Komariyah, Laili Larasati, Zu’ama Anggun Legowo, Djoko Lilik Maslachah Linaci, Ahmad Novta Sebad Listiyani Siti Romlah Luqman, Epy Muhammad M. Ihsan Dacholfany Maemunah, Atik Malintang, Jimmy Mardiani Diani, Mardiani Maria Ulfah Marikena, Nita Marlius, Farizal Mawadda, Novziah Mochamad Lazuardi Mufti Amir Sultan, Mufti Amir Muh. Darwis Muhamad Arif Muhammad Mustofa Muhtadin, Sabilal Mujib Mujib, Mujib Mukarohmah, Ayun Hafifatul Nabila, Rifa Jihan Nani Nagu Nasril, Nasril Netriwati Nopia, Eni Nurhidayat, Asep Endih Nuryanto Nuryanto Nusdianto Triakoso Octia, Aniesya Payung, Lidyawati Tandi Perangin-Angin, Halimatusakdiah Pujiyanto, Mahendra Ramada, Edo Ramli, Akhmad Repal, Joni Rina Budi Satiyarti, Rina Budi Rizka Febriani Rizki Ananda Rohmat Mulyana Sapdi Rusyawardani, Aldis Ingrid Saeful Bahri Saifuddin, Ismuddin B Saiful Bahri Salsabella Sutomo, Mudiah Sami, Abdul Santoso, Kuncoro Puguh Saputra, Rikky Triolin Sari, Anissa Rahma Sari, Indah Nur Bella Sari, Misalia SATRIYAS ILYAS Setyani, Jesika Sholihah, Nur Arifatus Shunhaji, Akhmad Siskandar Siskandar, Siskandar Siti Julaiha Siti Masitoh Siti Zulaikhah Sri Mulyati Suci Wulan Pawhestri Sujiyo Miranto, Sujiyo Sunardi, Dendi Suzanita Utama Syafitri, Qurnia Syaiful Anwar Syamsul Anwar, Syamsul Taufik Akbar Parluhutan Uswatun Hasanah Utami, Mulya Wahyuniar Wahyuniar Wasehudin, Wasehudin Wawan Wawan Widayanti Widayanti Widjiati Widjiati, Widjiati Xin, Irene Teh Kai Yama, Arifeen Yansyah, M. Eko Juli Yanti, Ipri Yudha Natakusuma, Bintang Yuniawati, Rizqy Aiddha Yustinasari, Lita Rakhma Zakia Al Erza, Rahma Zulfiani Zulfiani