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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Microbiology Indonesia Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Jurnal Teknologi Laboratorium Jurnal Medik Veteriner Oto Rhino Laryngologica Indonesiana Journal Orthopaedi and Traumatology Surabaya (JOINTS) Journal of Parasite Science Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Current Biomedicine
Fedik Abdul Rantam
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Characterization of Gene Coding Fusion Protein of Newcastle Disease Virus Infected in Native Chicken in Surabaya Kirana, Maha; Ernawati, Rahaju; Rahmahani, Jola; Rantam, Fedik Abdul
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.103-108

Abstract

This study aimed to discover the homology of nucleotide sequence, homology percentage, and those relations phylogenetic of protein Fusion (F) gene coding of Newcastle disease in domestic chicken (Gallus gallus domesticus) in Surabaya using some comparison isolate from GenBank. Samples were scoured of digestive organs from native chicken, that was collected from a traditional market in Wonokromo, Surabaya. Samples were tested using RT-PCR with primer forward and reverse with target 976bp, a positive sample which is continued with sequencing then homology and nucleotide analysis which is done and which is translated into amino acid. The result of homology chc/SBY/2018 sample has a similarity of 88% with references sequence, while with Lasota vaccine has a similarity of 87%, and the highest result of homology showed by the comparison with various isolates in Indonesia 90-95%. Translation results from nucleotide alignment into amino acid showed shifts in amino acid structure, which is amino acid shifts could be affected by many things like nutrition, wheater, environment, etc. The conclusion was chc/SBY/2018 sample has a quite high similarity with Indonesian isolates and undergoes mutation on nucleotide structure on amino acid and phylogenetic analysis. This study related to some isolates of vaccine and some isolates in Indonesia.
Serological Study of Newcastle Disease in Ducks (Anas javanicus) Slaughtered in East Surabaya Traditional Market Ardhanella, Sellianova; Damayanti, Ratna; Suwarno, Suwarno; Rantam, Fedik Abdul; Rachmawati, Kadek; Khairullah, Aswin Rafif; Rahmahani, Jola
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.131-137

Abstract

The purpose of this study was to determine the serological study of Newcastle Disease that infects slaughtered ducks (Anas javanicus) at the East Surabaya Traditional Market. This study was conducted from December 2018 to January 2019. A total of 122 samples were collected from four traditional markets in East Surabaya. The Haemagglutination Inhibition (HI) test was used to determine a positive result with an antibody titer of 4 or more. Samples for the HI test were collected from duck serum that had been treated with chicken red blood cells. The results showed 13 (10.65%) of the 122 samples were positive for ND.
Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method Rahmahani, Jola; Kirana, Maha; Rantam, Fedik Abdul; Suwarno, Suwarno
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.145-148

Abstract

This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B ( 5'-TAGTGATGMCGSGACATCAT-3' ) with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus.
Detection of Foot and Mouth Disease Virus in Cattle in Lamongan and Surabaya, Indonesia Using RT-PCR Method Dinana, Zayyin; Rantam, Fedik Abdul; Suwarno, Suwarno; Mustofa, Imam; Rahmahani, Jola; Kusnoto, Kusnoto
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.191-196

Abstract

Foot and mouth disease (FMD) virus was reported as an outbreak in Indonesia in April 2022 and belonged to serotype O/ME-SA/Ind-2001e is spread in the country. This study aimed to detect the causative agent based on clinical symptoms in cattle that have the vesicle in the mouth and hooves. A total of 25 samples were collected during August 2022 from Lamongan and Surabaya, Indonesia. FMD was identified in 58% (7/12) using reverse transcription polymerase chain reaction (RT-PCR), respectively. The samples were performed using universal primers with 328 bp length for primary diagnosis of FMD. These findings indicate that the spread of FMD viruses is highly contagious, so rapid and accurate diagnosis is needed as an effort to control and monitor FMD viruses.
PENGUJIAN VAKSIN HEPATITIS B FASE SUBKRONIS TERHADAP BERAT ORGAN DAN DIAMETER PULPA PUTIH LIMPA TIKUS PUTIH (Rattus norvegicus) Anindya, Afifatus; Santoso, Kuncoro Puguh; Rantam, Fedik Abdul; Rachmawati, Kadek; Nidom, Chairul Anwar; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20410

Abstract

This study aims to determine the effect of hepatitis B vaccine of spleen weight and white pulp diameter in rat (Rattus norvegicus). This study used 20 male wistar rats aged 8 weeks with an average weight of 200-250 grams, which was divided into four groups. Control group (P0) was given PBS solution, treatment group 1 (P1) was given type 1 hepatitis B vaccine, treatment group 2 was given type 2 hepatitis B vaccine, and treatment group 3 (P3) was given type 3 hepatitis B vaccine. Dose administered 0.4 ml and given repeatedly at days 0,30, and 60. Animals model to euthanize on day 75 for sampling. The spleen organ is weighed after the necropsing process and then the spleen is stored for the preparation of histopathologic with HE staining (Hematoxylin Eosin). The data obtained are weight data of spleen organ and spleen white pulp diameter. The results showed the highest weight of spleen organ 0.92 gram in group P0 and the largest white pulp diameter 34,34 μm in group P1. The results of data analyzed using one-way ANOVA (Analysis of Variance) and continued with Duncan alpha test. One Way Anova and Duncan alpha test showed that there were non significantly different between groups (p>0.05). The conclusion is we know that hepatitis B vaccine type 1,2, and 3 can't give the effect for spleen weight and white pulp diameter in rat (Rattus norvegicus).
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Evaluation and Its Impact of SARS-CoV-2 Inactivated Vaccine Candidate in K18-hACE2 Mice A'la, Rofiqul; Rantam, Fedik Abdul; Wijaya, Andi Yasmin; Susilowati, Helen; Kuncorojakti, Suryo; Diyantoro, Diyantoro; Aswin, Ahmad; Rahmahani, Jola; Suwanti, Lucia Tri; Lukiswanto, Bambang Sektiari; Yudaniayanti, Ira Sari; Setiawan, Boedi
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.54-62

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 requires effective vaccines to be developed. This study aimed to assess the impact of a SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice by monitoring their body weight, immune activation, and inflammatory cytokines including IL-4, IL-6, TNF-α, and IFN-γ. The study utilized k18-hACE2 mice expressing the human angiotensin-converting enzyme 2 (hACE2) receptor. The mice were administered the inactivated vaccine candidate compared with sham and vehicle. Body weight was monitored, and serum samples were collected to measure IL-4, IL-6, TNF-α, and IFN-γ levels using ELISA. Data were evaluated using SPSS statistical analysis software. The administration of the SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice did not result in significant changes in body weight compared to the control group. Furthermore, the levels of IL-4, IL-6, TNF-α, and IFN-γ were significantly reduced in the vaccinated mice compared to the control group, suggesting a dampening effect on the inflammatory response. This study demonstrates that the SARS-CoV-2 inactivated vaccine candidate has a minimal impact on the body weight of k18-hACE2 mice. Nevertheless, it successfully regulates the levels of IL-4, IL-6, TNF-α, and IFN-γ, suggesting its safety and beneficial impact. These findings contribute to understanding the vaccine's efficacy and safety profile in vaccine development.
Cytotoxicity of Kembang Bulan (Tithonia diversifolia ) Ethanolic Leaf Extract on Rabbit Limbal Mesenchymal Stem Cells Plumeriastuti, Hani; Zahro, Vida Seanita; Triakoso, Nusdianto; Rantam, Fedik Abdul; Hamid, Iwan Sahrial; Effendi, Mustofa Helmi; Proboningrat, Annise
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.233-239

Abstract

Kembang Bulan (Tithonia diversifolia ) is known as an herbal plant that has many useful properties to cure disease, such as diabetes, malaria, and other infections. Instead of much usefulness, T. diversifolia has main biological properties that may induce toxicity in this plant known as sesquiterpene lactones. The aim of this study was to identify the cytotoxicity effect of T. diversifolia leaf extract on limbal mesenchymal stem cells of rabbits using the MTT assay. T. diversifolia leaf extract was divided into 4 concentrations (0.5%, 0.25%, 0.125%, and 0.0625%) and given to limbal mesenchymal stem cells of rabbits in 96-well microplates, which would be incubated for 24 hours. The cytotoxicity result was obtained using the MTT assay method. Every well would be observed to see the cell life level of each concentration. The optical density absorbance was calculated using a microplate reader. T. diversifolia leaf extract with a concentration of 0.0625% has the biggest viability with a level of existence of limbal mesenchymal stem cells of 50%, and the other T. diversifolia leaf extract concentrations of 0.5%, 0.25%, and 0.125% have less than 50% level of existence of limbal mesenchymal stem cells.
Inhibition of Neurogenesis and Induction of Glial Scar Formation by Neuroinflammation Following Ischemic Stroke: Evaluation of BDNF, GFAP, HMGB1 and TNF-α Expressions Aris Widayati; Fedik Abdul Rantam; Abdulloh Machin; Wibi Riawan
The Indonesian Biomedical Journal Vol 17, No 1 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i1.3439

Abstract

BACKGROUND: Ischemic stroke remains as a major health problem and one important process in Ischemic Stroke is neuroinflammation which has a principal role to maintain the balance of neurogenesis and neurodegeneration process in the brain. Neuroinflammation can lead to glial scar and inhibit neurogenesis processes which is needed for recovery neuron function. This study was conducted to observe the role of high mobility group box 1 (HMGB1) and tumor necrosis factor-α (TNF-α) as neuroinflammation markers to glial fibrillary acidic protein (GFAP) as glial scar marker and to brain-derived neurotrophic factor (BDNF) as neurogenesis marker in brain tissue following ischemic stroke.METHODS: Fifteen male Wistar rats were randomized to three groups; sham group, rats receiving occlusion and terminated 180 minutes later (group A), and rats receiving occlusion and terminated after 7 days (group B). Expressions of BDNF and BDNF mRNA were examined using immunohistochemistry (IHC) and Reverse Transcription Polymerase Chain Reaction (RT-PCR), respectively. While GFAP, HMGB1, TNF-α were assessed using IHC.RESULTS: Expression of BDNF was found lower in group A and group B than in sham group (5.20±1.924, 5.00±1.581, and 7.80±1.304, respectively; p=0.032). Expression of BNDF mRNA was found lower in group A and B than in sham group as well. While expression of GFAP was found higher in group A and B than in sham group (9.60±1.517, 11.40±2.074, and 5.20±1.48, respectively; p=0.000). Higher level of HMGB1 and TNF-α expressions were also found to in group A and group B than in sham group (9.3±1.528, 11.67±1.528, and 2.00±1.000, respectively; p=0.000 for HMGB1 and 6.33±1.155, 9.33±1.528, and 3.00±1.000, respectively; p=0.002 for TNF-α).CONCLUSION: The presence of low BDNF levels and high levels of GFAP, HMGB1 and TNF-α markers, possibly reflects inhibition of the neurogenesis process by neuroinflammation, and induced glial scar formation in ischemic stroke conditions after than 180 hours until 7 days. KEYWORDS: ischemic stroke, BDNF, GFAP, TNF-α, HMGB1
Co-Authors . Helen . Mulyani . Purwati . PURWATI . Suwarno A'la, Rofiqul Abdurachman Abdurachman Agnes Theresia Soelih Estoepangestie Ahmad Chusnu Romdhoni Aksono HP., Eduardus Bimo Amaq Fadholly Ami Ashariati Amrullah, Amirul Muslim Andi Yasmin Wijaya Andriyanto Andriyanto Anindya, Afifatus Annise Proboningrat Ardhanella, Sellianova Arif Nur Muhammad Ansori Aris Widayati Aswin, Ahmad Aucky Hinting Aulia Andi Mustika Azahro, Aisyah Bambang Sektiari Lukiswanto Boedi Setiawan Candra Bumi Cita Rosita Sigit Prakoeswa Dadik Raharjo Damayanti Tinduh Desi Sandra Sari Diah Nugrahani Pristihadi Didik Handijatno Diyantoro Diyantoro, Diyantoro Dwikora Novembri Utomo Emmanuel Djoko Poetranto ENDANG RETNOWATI KUSUMOWIDAGDO Endang Suprihati ERMA SAFITRI Ernawati, Rahayu Ernie Maduratna Ferdiansyah Ferdiansyah Mahyudin Gilang Maulana Putra HAK HOTTA Hani Plumeriastuti Hari Basuki Notobroto Helen Susilowati, Helen Henry Dominica Heri Suroto HERNOMO ONTOSENO KUSUMOBROTO I'tishom, Reny Ika WAHYUNI IMAM MUSTOFA Imam Mustofa Indah Rahmawati Ira Sari Yudaniayanti Iwan Sahrial Hamid Juniastuti Juniastuti Kadek Rachmawati Khairullah, Aswin Rafif Kharisma, Viol Dhea Kirana, Maha Kuncorojakti, Suryo Kusnoto Kusnoto, Kusnoto Laba Mahaputra Lina Noviyanti Sutardi Lucia Tri Suwanti, Lucia Tri Lukman Hakim Machin, Abdulloh MARIA INGE LUSIDA Mas’ud Hariadi Mouli Edward Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nasronudin Nasronudin Nenny Harijani Ni Ketut Suwiti Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nusdianto Triakoso Purwati Purwati Putih, Nurvita R. Heru Prasetyo Rahaju Ernawati Rahmahani, Jola Ratih Ratnasari Ratna Damayanti Retno Pudji Rahayu Ria Hanistya Rina Mutya Suzliana Romaniyanto Romaniyanto Romaniyanto Romy Muhammad Dary Mufa S.Pd. M Kes I Ketut Sudiana . Santoso, Kuncoro Puguh SOETJIPTO . Sri Hernawati Sri Pantja Madyawati Sri Wigati Mardi Steven Sheng Looi Suwarno . Suwarno Suwarno Suzanita Utama Tacharina, Martia Rani TAKAKO UTSUMI Tristiana Erawati Munandar Tyasningsih, Wiwiek Venri Novryantoro Wibi Riawan Widiyatno, Thomas Valentinus Widji Soeratri Widjiati Widjiati, Widjiati Widodo Ario Kentjono Wijaya, Andi Yasmin Winona May Hendrata Wulandari, Innah Yoes Priyatna Dachlan YOSHIHIKO YANO YOSHITAKE HAYASHI Yulanda Antonius Yusuf, Muhtarum Zahro, Vida Seanita Zayyin Dinana