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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Microbiology Indonesia Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Jurnal Teknologi Laboratorium Jurnal Medik Veteriner Oto Rhino Laryngologica Indonesiana Journal Orthopaedi and Traumatology Surabaya (JOINTS) Journal of Parasite Science Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Current Biomedicine
Fedik Abdul Rantam
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Isolation and Biological Characterization of Newcastle Disease Virus (NDV) Field Isolate Pigeon (Columba livia) from Live Bird Market, East Java in 2019 Venri Novryantoro; Fedik Abdul Rantam; Suwarno Suwarno; Rahaju Ernawati; Jola Rahmahani
Journal of Applied Veterinary Science And Technology Vol. 4 No. 2 (2023): October 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I2.2023.67-73

Abstract

Background: Avian Paramyxovirus (APMV) type-1 is the leading cause of Newcastle Disease (ND) and taxonomically belongs to the family Paramyxoviridae, genus Avulavirus. Due to its high transmission rate, Newcastle Disease (ND) is included in the A list by the OIE. Purpose: To determine the biological characterize the Newcastle Disease Virus (NDV) field isolate of pigeons (Columba livia) using Mean Death Time (MDT), Intracerebral Pathogenicity Index (ICPI), and Intravenous Pathogenicity Index (IVPI). Methods: Twenty pigeon organ samples were obtained from bird markets in East Java, and one was used as a positive control (LaSota). Organs were isolated from embryonated chicken eggs, identified by the HA test, and then confirmed by the HI test. Furthermore, positive samples were tested for MDT with a 10-1-10-18 dilution (0.1 ml and observed for eight days. The ICPI test used a fresh NDV suspension (0.05 ml and was observed for eight days. The IVPI test used a dose of 0.1 ml and was observed for ten days. Result: The MDT values of isolates MB1/NDV/19, MB2/NDV/19, MB3/NDV/19, and MG1/NDV/19 were 91.2 hours, 112.8 hours, 110.4 hours, and 124,8 hours. The ICPI values of isolate MB1/NDV/19 was 0.2375, MB2/NDV/19 was 0.375, MB3/NDV/19 was 0.5375, and MG1/NDV/19 was 0.3. The IVPI value of isolate MB1/NDV/19 was 0, MB2/NDV/19 was 0, MB3/NDV/19 was 0, and MG1/NDV/19 was 0. Conclusion: All four field samples were positive for NDV as a lentogenic strain based on the MDT, ICPI, and IVPI tests.
Uji sitotoksik ekstrak cabai merah keriting (Capsicum annuum) pada sel WiDr secara in vitro Fadholly, Amaq; Sudjarwo, Sri Agus; Rantam, Fedik Abdul; Mustika, Aulia Andi; Andriyanto, Andriyanto; Pristihadi, Diah Nugrahani; Sutardi, Lina Noviyanti
Current Biomedicine Vol. 1 No. 2 (2023): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.2.70-75

Abstract

Latar belakang: Kanker masih menjadi salah satu penyebab kematian di dunia. Salah satu alternatif metode untuk mengobati kanker adalah dengan obat herbal yang mempunyai khasiat sebagai antikanker. Tujuan: Penelitian ini bertujuan untuk manganalisis efek sitotoksik esktrak Capsium annuum pada sel WiDr. Metode: Kemampuan sitotoksik ekstrak Capsium annuum diuji secara in vitro pada sel WiDr menggunakan metode MTT tetrazolium reduction assay. Hasil: Hasil uji MTT menunjukkan bahwa ekstrak Capsicum annuum menghambat proliferasi sel WiDr seiring dengan peningkatan konsentrasi (400, 800, dan 1600 ppm), dengan persentase penghambatan sel tertinggi diperoleh pada konsentrasi 800 ppm dari setiap kelompok terapi 24, 48, dan 72 jam. Nilai inhibitory concentration 50 (IC50) terendah dihasilkan oleh kelompok waktu terapi 48 jam, yaitu 651,18 ppm. Simpulan: Nilai IC50 ekstrak Capsicum annuum untuk sel WiDr memiliki nilai yang tinggi yang menunjukkan belum efektif sebagai obat antikanker. Penelitian lebih lanjut dibutuhkan untuk mendapatkan dosis terbaik agar dapat menghambat pertumbuhan sel WiDr secara maksimal.
CHARACTERIzATION of CD4 + T LYMPHOCYTE FROM BONE MAROW STEM CELL USING INDIRECT IMMUNOFLUORESENCE FOR HIV & AIDS TREATMENT Purwati, Purwati; Nasronudin, Nasronudin; Rantam, Fedik Abdul
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 3 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2298.797 KB) | DOI: 10.20473/ijtid.v1i3.2195

Abstract

Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HIV). At the beginning of infection, gp120 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gp120 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell. Therefore, CCR5 and CXCR4 also determine the fate of the target cell. It is the performance of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HIV infection. Coding gene CCR5 may mutate to become protective or resistant against HIV infection. In homozygote individuals, it tends to be resistant against infection, while in heterozygote individuals it tends to be susceptible to HIV infection. Objective: To characterize TCD4 lymphocyte in the next that is resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HIV and comparison to control. Result: This study was performed in several steps, such as mononucleated cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification. Conclusion: Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment.
Molecular Characterization of Hemagglutinin-Neuraminidase Protein Virus Newcastle disease (ND) in Surabaya during 2003 and 2016 Wulandari, Innah; Rahmahani, Jola; Rahmawati, Indah; Putih, Nurvita; Azahro, Aisyah; Kusnoto, Kusnoto; Ernawati, Rahayu; Rantam, Fedik Abdul
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.231-242

Abstract

This study aimed to determine the mutation of amino acid, nucleotide homology, phylogenetic tree, epitope prediction of Hemagglutinin-Neuraminidase protein Newcastle Disease (ND) Virus isolated from traditional market around Surabaya. Samples were from 37 chicken with cloacal swab and one positive samples for control (LaSota). Samples were inoculated on embyonate chicken eggs and identified with HA test confirmed with HI test. Positive samples processed by PCR using forward and reverse primer with 503 bp RNA target. The PCR result then analyzed with sequencing. Result of sequencing analysis showed that theres similiarity between samples amino acid and vaccine isolate its effect the percentage of nucleotide homology and phylogenetic relation between isolate. Epitope NGAANNSGWGAPIHDPDYIGG have high immunogenic value at all of isolate which good as vaccine candidate.
Characterization of Gene Coding Fusion Protein of Newcastle Disease Virus Infected in Native Chicken in Surabaya Kirana, Maha; Ernawati, Rahaju; Rahmahani, Jola; Rantam, Fedik Abdul
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.103-108

Abstract

This study aimed to discover the homology of nucleotide sequence, homology percentage, and those relations phylogenetic of protein Fusion (F) gene coding of Newcastle disease in domestic chicken (Gallus gallus domesticus) in Surabaya using some comparison isolate from GenBank. Samples were scoured of digestive organs from native chicken, that was collected from a traditional market in Wonokromo, Surabaya. Samples were tested using RT-PCR with primer forward and reverse with target 976bp, a positive sample which is continued with sequencing then homology and nucleotide analysis which is done and which is translated into amino acid. The result of homology chc/SBY/2018 sample has a similarity of 88% with references sequence, while with Lasota vaccine has a similarity of 87%, and the highest result of homology showed by the comparison with various isolates in Indonesia 90-95%. Translation results from nucleotide alignment into amino acid showed shifts in amino acid structure, which is amino acid shifts could be affected by many things like nutrition, wheater, environment, etc. The conclusion was chc/SBY/2018 sample has a quite high similarity with Indonesian isolates and undergoes mutation on nucleotide structure on amino acid and phylogenetic analysis. This study related to some isolates of vaccine and some isolates in Indonesia.
Serological Study of Newcastle Disease in Ducks (Anas javanicus) Slaughtered in East Surabaya Traditional Market Ardhanella, Sellianova; Damayanti, Ratna; Suwarno, Suwarno; Rantam, Fedik Abdul; Rachmawati, Kadek; Khairullah, Aswin Rafif; Rahmahani, Jola
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.131-137

Abstract

The purpose of this study was to determine the serological study of Newcastle Disease that infects slaughtered ducks (Anas javanicus) at the East Surabaya Traditional Market. This study was conducted from December 2018 to January 2019. A total of 122 samples were collected from four traditional markets in East Surabaya. The Haemagglutination Inhibition (HI) test was used to determine a positive result with an antibody titer of 4 or more. Samples for the HI test were collected from duck serum that had been treated with chicken red blood cells. The results showed 13 (10.65%) of the 122 samples were positive for ND.
Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method Rahmahani, Jola; Kirana, Maha; Rantam, Fedik Abdul; Suwarno, Suwarno
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.145-148

Abstract

This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B ( 5'-TAGTGATGMCGSGACATCAT-3' ) with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus.
Detection of Foot and Mouth Disease Virus in Cattle in Lamongan and Surabaya, Indonesia Using RT-PCR Method Dinana, Zayyin; Rantam, Fedik Abdul; Suwarno, Suwarno; Mustofa, Imam; Rahmahani, Jola; Kusnoto, Kusnoto
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.191-196

Abstract

Foot and mouth disease (FMD) virus was reported as an outbreak in Indonesia in April 2022 and belonged to serotype O/ME-SA/Ind-2001e is spread in the country. This study aimed to detect the causative agent based on clinical symptoms in cattle that have the vesicle in the mouth and hooves. A total of 25 samples were collected during August 2022 from Lamongan and Surabaya, Indonesia. FMD was identified in 58% (7/12) using reverse transcription polymerase chain reaction (RT-PCR), respectively. The samples were performed using universal primers with 328 bp length for primary diagnosis of FMD. These findings indicate that the spread of FMD viruses is highly contagious, so rapid and accurate diagnosis is needed as an effort to control and monitor FMD viruses.
PENGUJIAN VAKSIN HEPATITIS B FASE SUBKRONIS TERHADAP BERAT ORGAN DAN DIAMETER PULPA PUTIH LIMPA TIKUS PUTIH (Rattus norvegicus) Anindya, Afifatus; Santoso, Kuncoro Puguh; Rantam, Fedik Abdul; Rachmawati, Kadek; Nidom, Chairul Anwar; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20410

Abstract

This study aims to determine the effect of hepatitis B vaccine of spleen weight and white pulp diameter in rat (Rattus norvegicus). This study used 20 male wistar rats aged 8 weeks with an average weight of 200-250 grams, which was divided into four groups. Control group (P0) was given PBS solution, treatment group 1 (P1) was given type 1 hepatitis B vaccine, treatment group 2 was given type 2 hepatitis B vaccine, and treatment group 3 (P3) was given type 3 hepatitis B vaccine. Dose administered 0.4 ml and given repeatedly at days 0,30, and 60. Animals model to euthanize on day 75 for sampling. The spleen organ is weighed after the necropsing process and then the spleen is stored for the preparation of histopathologic with HE staining (Hematoxylin Eosin). The data obtained are weight data of spleen organ and spleen white pulp diameter. The results showed the highest weight of spleen organ 0.92 gram in group P0 and the largest white pulp diameter 34,34 μm in group P1. The results of data analyzed using one-way ANOVA (Analysis of Variance) and continued with Duncan alpha test. One Way Anova and Duncan alpha test showed that there were non significantly different between groups (p>0.05). The conclusion is we know that hepatitis B vaccine type 1,2, and 3 can't give the effect for spleen weight and white pulp diameter in rat (Rattus norvegicus).
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Co-Authors . Helen . Mulyani . Purwati . PURWATI . Suwarno A'la, Rofiqul Abdurachman Abdurachman Ahmad Chusnu Romdhoni Aksono HP., Eduardus Bimo Amaq Fadholly Ami Ashariati Amrullah, Amirul Muslim Andi Yasmin Wijaya Andriyanto Andriyanto Anindya, Afifatus Annise Proboningrat Ardhanella, Sellianova Arif Nur Muhammad Ansori Aris Widayati Aswin, Ahmad Aucky Hinting Aulia Andi Mustika Azahro, Aisyah Bambang Sektiari Lukiswanto Boedi Setiawan Candra Bumi Cita Rosita Sigit Prakoeswa Damayanti Tinduh Desi Sandra Sari Diah Nugrahani Pristihadi Didik Handijatno Diyantoro Diyantoro, Diyantoro Dwikora Novembri Utomo Emmanuel Djoko Poetranto ENDANG RETNOWATI KUSUMOWIDAGDO Endang Suprihati ERMA SAFITRI Ernawati, Rahaju Ernawati, Rahayu Ernie Maduratna Estoepangestie, Agnes Theresia Soelih Ferdiansyah Ferdiansyah Mahyudin Gilang Maulana Putra HAK HOTTA Hani Plumeriastuti Hari Basuki Notobroto Harijani, Nenny Helen Susilowati, Helen Henry Dominica Heri Suroto HERNOMO ONTOSENO KUSUMOBROTO I'tishom, Reny Ika WAHYUNI IMAM MUSTOFA Imam Mustofa Indah Rahmawati Ira Sari Yudaniayanti Iwan Sahrial Hamid Juniastuti Juniastuti Kadek Rachmawati Khairullah, Aswin Rafif Kharisma, Viol Dhea Kirana, Maha Kuncorojakti, Suryo Kusnoto Kusnoto, Kusnoto Laba Mahaputra Lina Noviyanti Sutardi Lucia Tri Suwanti, Lucia Tri Lukman Hakim Machin, Abdulloh MARIA INGE LUSIDA Mas’ud Hariadi Mouli Edward Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nasronudin Nasronudin Ni Ketut Suwiti Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nusdianto Triakoso Purwati Purwati Putih, Nurvita R. Heru Prasetyo Rahaju Ernawati Raharjo, Dadik Rahmahani, Jola Ratih Ratnasari Ratna Damayanti Retno Pudji Rahayu Ria Hanistya Rina Mutya Suzliana Romaniyanto Romaniyanto Romaniyanto Romy Muhammad Dary Mufa S.Pd. M Kes I Ketut Sudiana . Santoso, Kuncoro Puguh SOETJIPTO . Sri Hernawati Sri Pantja Madyawati Sri Wigati Mardi Steven Sheng Looi Suwarno . Suwarno Suwarno Suwarno Suwarno Suzanita Utama Tacharina, Martia Rani TAKAKO UTSUMI Tristiana Erawati Tyasningsih, Wiwiek Venri Novryantoro Wibi Riawan Widiyatno, Thomas Valentinus Widji Soeratri Widjiati . Widjiati Widjiati Widodo Ario Kentjono Wijaya, Andi Yasmin Winona May Hendrata Wulandari, Innah Yoes Priyatna Dachlan YOSHIHIKO YANO YOSHITAKE HAYASHI Yulanda Antonius Yusuf, Muhtarum Zahro, Vida Seanita Zayyin Dinana