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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Microbiology Indonesia Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Jurnal Teknologi Laboratorium Jurnal Medik Veteriner Oto Rhino Laryngologica Indonesiana Journal Orthopaedi and Traumatology Surabaya (JOINTS) Journal of Parasite Science Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Current Biomedicine
Fedik Abdul Rantam
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Evaluation and Its Impact of SARS-CoV-2 Inactivated Vaccine Candidate in K18-hACE2 Mice A'la, Rofiqul; Rantam, Fedik Abdul; Wijaya, Andi Yasmin; Susilowati, Helen; Kuncorojakti, Suryo; Diyantoro, Diyantoro; Aswin, Ahmad; Rahmahani, Jola; Suwanti, Lucia Tri; Lukiswanto, Bambang Sektiari; Yudaniayanti, Ira Sari; Setiawan, Boedi
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.54-62

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 requires effective vaccines to be developed. This study aimed to assess the impact of a SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice by monitoring their body weight, immune activation, and inflammatory cytokines including IL-4, IL-6, TNF-α, and IFN-γ. The study utilized k18-hACE2 mice expressing the human angiotensin-converting enzyme 2 (hACE2) receptor. The mice were administered the inactivated vaccine candidate compared with sham and vehicle. Body weight was monitored, and serum samples were collected to measure IL-4, IL-6, TNF-α, and IFN-γ levels using ELISA. Data were evaluated using SPSS statistical analysis software. The administration of the SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice did not result in significant changes in body weight compared to the control group. Furthermore, the levels of IL-4, IL-6, TNF-α, and IFN-γ were significantly reduced in the vaccinated mice compared to the control group, suggesting a dampening effect on the inflammatory response. This study demonstrates that the SARS-CoV-2 inactivated vaccine candidate has a minimal impact on the body weight of k18-hACE2 mice. Nevertheless, it successfully regulates the levels of IL-4, IL-6, TNF-α, and IFN-γ, suggesting its safety and beneficial impact. These findings contribute to understanding the vaccine's efficacy and safety profile in vaccine development.
Cytotoxicity of Kembang Bulan (Tithonia diversifolia ) Ethanolic Leaf Extract on Rabbit Limbal Mesenchymal Stem Cells Plumeriastuti, Hani; Zahro, Vida Seanita; Triakoso, Nusdianto; Rantam, Fedik Abdul; Hamid, Iwan Sahrial; Effendi, Mustofa Helmi; Proboningrat, Annise
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.233-239

Abstract

Kembang Bulan (Tithonia diversifolia ) is known as an herbal plant that has many useful properties to cure disease, such as diabetes, malaria, and other infections. Instead of much usefulness, T. diversifolia has main biological properties that may induce toxicity in this plant known as sesquiterpene lactones. The aim of this study was to identify the cytotoxicity effect of T. diversifolia leaf extract on limbal mesenchymal stem cells of rabbits using the MTT assay. T. diversifolia leaf extract was divided into 4 concentrations (0.5%, 0.25%, 0.125%, and 0.0625%) and given to limbal mesenchymal stem cells of rabbits in 96-well microplates, which would be incubated for 24 hours. The cytotoxicity result was obtained using the MTT assay method. Every well would be observed to see the cell life level of each concentration. The optical density absorbance was calculated using a microplate reader. T. diversifolia leaf extract with a concentration of 0.0625% has the biggest viability with a level of existence of limbal mesenchymal stem cells of 50%, and the other T. diversifolia leaf extract concentrations of 0.5%, 0.25%, and 0.125% have less than 50% level of existence of limbal mesenchymal stem cells.
Inhibition of Neurogenesis and Induction of Glial Scar Formation by Neuroinflammation Following Ischemic Stroke: Evaluation of BDNF, GFAP, HMGB1 and TNF-α Expressions Widayati, Aris; Rantam, Fedik Abdul; Machin, Abdulloh; Riawan, Wibi
The Indonesian Biomedical Journal Vol 17, No 1 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i1.3439

Abstract

BACKGROUND: Ischemic stroke remains as a major health problem and one important process in Ischemic Stroke is neuroinflammation which has a principal role to maintain the balance of neurogenesis and neurodegeneration process in the brain. Neuroinflammation can lead to glial scar and inhibit neurogenesis processes which is needed for recovery neuron function. This study was conducted to observe the role of high mobility group box 1 (HMGB1) and tumor necrosis factor-α (TNF-α) as neuroinflammation markers to glial fibrillary acidic protein (GFAP) as glial scar marker and to brain-derived neurotrophic factor (BDNF) as neurogenesis marker in brain tissue following ischemic stroke.METHODS: Fifteen male Wistar rats were randomized to three groups; sham group, rats receiving occlusion and terminated 180 minutes later (group A), and rats receiving occlusion and terminated after 7 days (group B). Expressions of BDNF and BDNF mRNA were examined using immunohistochemistry (IHC) and Reverse Transcription Polymerase Chain Reaction (RT-PCR), respectively. While GFAP, HMGB1, TNF-α were assessed using IHC.RESULTS: Expression of BDNF was found lower in group A and group B than in sham group (5.20±1.924, 5.00±1.581, and 7.80±1.304, respectively; p=0.032). Expression of BNDF mRNA was found lower in group A and B than in sham group as well. While expression of GFAP was found higher in group A and B than in sham group (9.60±1.517, 11.40±2.074, and 5.20±1.48, respectively; p=0.000). Higher level of HMGB1 and TNF-α expressions were also found to in group A and group B than in sham group (9.3±1.528, 11.67±1.528, and 2.00±1.000, respectively; p=0.000 for HMGB1 and 6.33±1.155, 9.33±1.528, and 3.00±1.000, respectively; p=0.002 for TNF-α).CONCLUSION: The presence of low BDNF levels and high levels of GFAP, HMGB1 and TNF-α markers, possibly reflects inhibition of the neurogenesis process by neuroinflammation, and induced glial scar formation in ischemic stroke conditions after than 180 hours until 7 days. KEYWORDS: ischemic stroke, BDNF, GFAP, TNF-α, HMGB1
Co-Authors . Helen . Mulyani . Purwati . PURWATI . Suwarno A'la, Rofiqul Abdurachman Abdurachman Ahmad Chusnu Romdhoni Aksono HP., Eduardus Bimo Amaq Fadholly Ami Ashariati Amrullah, Amirul Muslim Andi Yasmin Wijaya Andriyanto Andriyanto Anindya, Afifatus Annise Proboningrat Ardhanella, Sellianova Arif Nur Muhammad Ansori Aris Widayati Aswin, Ahmad Aucky Hinting Aulia Andi Mustika Azahro, Aisyah Bambang Sektiari Lukiswanto Boedi Setiawan Candra Bumi Cita Rosita Sigit Prakoeswa Damayanti Tinduh Desi Sandra Sari Diah Nugrahani Pristihadi Didik Handijatno Diyantoro Diyantoro, Diyantoro Dwikora Novembri Utomo Emmanuel Djoko Poetranto ENDANG RETNOWATI KUSUMOWIDAGDO Endang Suprihati ERMA SAFITRI Ernawati, Rahaju Ernawati, Rahayu Ernie Maduratna Estoepangestie, Agnes Theresia Soelih Ferdiansyah Ferdiansyah Mahyudin Gilang Maulana Putra HAK HOTTA Hani Plumeriastuti Hari Basuki Notobroto Harijani, Nenny Helen Susilowati, Helen Henry Dominica Heri Suroto HERNOMO ONTOSENO KUSUMOBROTO I'tishom, Reny Ika WAHYUNI IMAM MUSTOFA Imam Mustofa Indah Rahmawati Ira Sari Yudaniayanti Iwan Sahrial Hamid Juniastuti Juniastuti Kadek Rachmawati Khairullah, Aswin Rafif Kharisma, Viol Dhea Kirana, Maha Kuncorojakti, Suryo Kusnoto Kusnoto, Kusnoto Laba Mahaputra Lina Noviyanti Sutardi Lucia Tri Suwanti, Lucia Tri Lukman Hakim Machin, Abdulloh MARIA INGE LUSIDA Mas’ud Hariadi Mouli Edward Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nasronudin Nasronudin Ni Ketut Suwiti Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nusdianto Triakoso Purwati Purwati Putih, Nurvita R. Heru Prasetyo Rahaju Ernawati Raharjo, Dadik Rahmahani, Jola Ratih Ratnasari Ratna Damayanti Retno Pudji Rahayu Ria Hanistya Rina Mutya Suzliana Romaniyanto Romaniyanto Romaniyanto Romy Muhammad Dary Mufa S.Pd. M Kes I Ketut Sudiana . Santoso, Kuncoro Puguh SOETJIPTO . Sri Hernawati Sri Pantja Madyawati Sri Wigati Mardi Steven Sheng Looi Suwarno . Suwarno Suwarno Suwarno Suwarno Suzanita Utama Tacharina, Martia Rani TAKAKO UTSUMI Tristiana Erawati Tyasningsih, Wiwiek Venri Novryantoro Wibi Riawan Widiyatno, Thomas Valentinus Widji Soeratri Widjiati . Widjiati Widjiati Widodo Ario Kentjono Wijaya, Andi Yasmin Winona May Hendrata Wulandari, Innah Yoes Priyatna Dachlan YOSHIHIKO YANO YOSHITAKE HAYASHI Yulanda Antonius Yusuf, Muhtarum Zahro, Vida Seanita Zayyin Dinana